Standard name
Human Ortholog
Description Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.24 0.14 0.13 0.09 0.17 0.34 0.33 – 0.32 0.37 0.42 0.35 0.09 0.11 0.05 0.12 0.07 0 0.08 0.06 0 0.06 0 0
Bud 0 0 0 0 0 0.09 0 – 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.76 0.82 0.85 0.78 0.75 0.49 0.69 – 0.51 0.63 0.63 0.61 0.93 0.89 0.93 0.89 0.89 0.87 0.8 0.77 0.91 0.83 0.88 0.9
Cytoplasm 0.26 0.35 0.15 0.43 0.18 0.27 0.16 – 0.18 0.2 0.19 0.19 0.21 0.15 0.08 0.09 0.15 0.11 0 0.1 0 0 0 0
Endoplasmic Reticulum 0.11 0 0 0.05 0.09 0 0.1 – 0 0.07 0.07 0.06 0.07 0.05 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.07 – 0 0 0 0.07 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.33 0 – 0.33 0.2 0.32 0.27 0 0 0 0.12 0.11 0.16 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 59 22 11 14 28 40 100 – 24 77 113 68 24 46 23 12 9 6 22 11 1 10 16 14
Bud 9 7 2 1 5 10 6 – 2 6 15 8 4 8 6 1 0 2 0 0 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 4 2 0 2 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 187 131 72 118 123 57 208 – 39 132 170 120 236 372 411 91 110 118 219 139 99 158 373 340
Cytoplasm 63 56 13 65 30 31 47 – 14 42 52 37 54 61 36 9 18 15 11 18 4 4 10 2
Endoplasmic Reticulum 28 2 3 8 14 4 30 – 3 15 18 11 19 20 9 6 1 5 12 2 0 3 10 6
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 2
Golgi 0 0 0 0 2 1 22 – 0 3 4 14 1 1 0 1 6 1 1 0 0 0 0 0
Mitochondria 1 1 0 0 0 38 11 – 25 41 86 52 0 0 1 12 14 22 1 0 0 7 2 1
Nucleus 0 0 0 0 0 2 2 – 3 1 4 1 0 0 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 – 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 2 1 – 0 0 1 1 0 1 0 1 0 1 0 0 0 0 0 0
SpindlePole 0 1 2 0 0 2 2 – 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 1 7 – 0 1 1 7 0 0 0 2 2 0 0 1 0 1 0 0
Unique Cell Count 246 160 85 151 163 116 302 76 208 268 196 254 419 441 102 124 135 276 181 109 192 423 376
Labelled Cell Count 348 221 103 207 202 188 436 110 318 465 321 338 509 487 136 160 170 276 181 109 192 423 376


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.0 13.7 14.5 13.7 16.2 10.9 15.1 – 12.0 13.4 12.8 13.9 18.7 21.1 21.5 22.1 23.4 22.6 13.4 14.9 16.0
Std Deviation (1e-4) 2.2 2.2 2.0 2.2 2.8 2.6 2.4 – 2.8 3.0 2.7 4.0 2.9 3.7 4.2 4.8 9.6 4.8 3.0 2.8 3.1
Intensity Change (Log2) – – – -0.08 0.16 -0.41 0.06 – -0.27 -0.11 -0.18 -0.06 0.36 0.54 0.56 0.61 0.69 0.64 -0.12 0.04 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 0.9 3.5 3.6 – 2.9 4.1 4.9 3.7 -0.9 -0.5 -2.7 -0.2 -1.4 -2.3
Bud 0 0 1.9 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.2 -1.7 -5.2 -2.9 – -4.6 -3.6 -3.7 -3.9 2.3 1.1 2.6 0.9 0.8 0.6
Cytoplasm 4.4 0.6 1.9 0.1 – 0.5 1.0 0.9 0.7 1.2 -0.2 -2.1 -1.4 -0.2 -0.9
Endoplasmic Reticulum 0 1.5 0 1.9 – 0 1.2 1.1 0 1.3 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 0 – 5.8 4.4 6.0 5.3 0 0 0 3.3 3.2 3.9
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.5854 14.1796 11.5235 9.8763 10.5633 11.6054 14.4703 19.3522 16.7638 15.3815 14.686 16.8912 18.0905 21.6781 19.5211 17.9984 20.2378 19.291
Actin 0.0353 0.0108 0.0122 0.0017 0.0054 0.0036 0.0377 0.0005 0.0061 0.0088 0.0125 0.0131 0.0052 0.0014 0.0075 0.0007 0.0027 0.0021
Bud 0.0014 0.0003 0.0004 0.0001 0.0006 0.0002 0.0007 0.0001 0.0005 0.0004 0.0006 0.0005 0.0006 0.0002 0.0002 0.0003 0.0003 0.0003
Bud Neck 0.0019 0.0007 0.0021 0.0006 0.0036 0.0018 0.0016 0.001 0.0023 0.0044 0.0013 0.0023 0.0024 0.0017 0.0039 0.0072 0.003 0.0032
Bud Periphery 0.009 0.0012 0.0025 0.0017 0.0041 0.0033 0.0042 0.0007 0.0017 0.0069 0.0059 0.005 0.0037 0.001 0.0019 0.0049 0.0032 0.0053
Bud Site 0.0095 0.0379 0.0089 0.0004 0.0051 0.0004 0.0063 0.0108 0.0138 0.0013 0.0024 0.0011 0.0076 0.0371 0.0076 0.001 0.0015 0.0008
Cell Periphery 0.7132 0.8318 0.8257 0.8328 0.7714 0.8619 0.6997 0.779 0.7695 0.7733 0.7822 0.8051 0.8039 0.7744 0.8452 0.8007 0.7483 0.8302
Cytoplasm 0.0814 0.0513 0.0679 0.0264 0.1116 0.0442 0.087 0.1601 0.1231 0.0824 0.0556 0.0911 0.0986 0.1486 0.084 0.1176 0.1901 0.1004
Cytoplasmic Foci 0.0102 0.0013 0.0047 0.0031 0.0012 0.0006 0.0035 0.0001 0.0022 0.0184 0.0008 0.0004 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001
Eisosomes 0.0604 0.0322 0.0323 0.0061 0.0439 0.04 0.0774 0.0252 0.0232 0.0168 0.0885 0.0252 0.0247 0.0241 0.0216 0.0062 0.0312 0.0253
Endoplasmic Reticulum 0.025 0.0235 0.0131 0.0243 0.0187 0.0186 0.0315 0.0187 0.0211 0.0508 0.0315 0.0326 0.0372 0.008 0.021 0.0532 0.0153 0.0273
Endosome 0.0064 0.0003 0.0019 0.0059 0.0004 0.0023 0.0045 0 0.0017 0.001 0.0008 0.0013 0.0019 0 0.0002 0 0 0
Golgi 0.0044 0.0015 0.0013 0.0013 0.0006 0.0019 0.0089 0.0002 0.0012 0.0015 0.0015 0.0021 0.0005 0.0003 0.0004 0.0003 0.0003 0.0004
Lipid Particles 0.0067 0.0026 0.004 0.0113 0.0042 0.0014 0.0037 0.0004 0.005 0.0026 0.0014 0.0012 0.0012 0.0004 0.0008 0.0009 0.0004 0.0008
Mitochondria 0.0168 0 0.0126 0.0376 0.0175 0.0118 0.0123 0 0.017 0.0227 0.0083 0.0149 0.002 0 0.0003 0 0 0
None 0.0025 0.0004 0.0039 0.0016 0.0008 0.0005 0.0013 0.0003 0.0018 0.0007 0.0015 0.0003 0.0008 0.0003 0.0004 0.0002 0.0003 0.0002
Nuclear Periphery 0.0038 0.0017 0.0014 0.0058 0.001 0.0046 0.0099 0.0016 0.0015 0.0024 0.0019 0.0015 0.0046 0.0012 0.0023 0.0034 0.0018 0.0014
Nucleolus 0.0013 0.0001 0.0002 0.0265 0.0014 0.0001 0.0003 0 0.001 0.0001 0.0001 0 0.0001 0 0 0 0 0
Nucleus 0.0013 0.0002 0.0002 0.0017 0.0006 0.0003 0.0028 0.0001 0.0007 0.0002 0.0002 0.0001 0.0005 0.0001 0.0002 0.0002 0.0001 0.0001
Peroxisomes 0.0024 0 0.0024 0.0037 0.0055 0.0002 0.0015 0 0.0042 0.0017 0.0005 0.0003 0 0 0.0002 0 0 0
Punctate Nuclear 0.0019 0.0001 0.0004 0.0037 0.0005 0.0003 0.0004 0 0.0004 0.0011 0 0.0001 0.0002 0 0.0001 0 0 0
Vacuole 0.0046 0.002 0.0019 0.0028 0.0018 0.0016 0.004 0.0013 0.0019 0.0023 0.0024 0.0019 0.0036 0.0011 0.0019 0.0029 0.0012 0.0019
Vacuole Periphery 0.0007 0 0.0002 0.0008 0.0002 0.0003 0.0007 0 0.0002 0.0001 0.0003 0.0002 0.0004 0 0.0001 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.8813 42.0729 75.1591 84.5669 72.3216 97.5615 63.8098 95.7052 125.1709 73.1196
Translational Efficiency 1.698 1.7757 1.4472 2.2059 1.5431 1.287 1.6091 1.4924 1.325 1.4639

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (71%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-2

Rfs1

Rfs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available