Standard name
Human Ortholog
Description Splicing factor; component of the U4/U6-U5 snRNP complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0.11 0.08 0.17 0.11 0.11 0.17 0.06 0.05 0.08 0.06 0.09 0.14 0.14 0.12 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.17 0.07 0 0 0.14 0.35 0.27 0.33 0.65 0.53 0.34 0.07 0 0 0.09 0.05 0.13 0 0 0 0 0 0
Nucleus 0.93 0.92 0.9 0.87 0.85 0.73 0.86 0.76 0.75 0.68 0.73 0.85 0.92 0.89 0.84 0.81 0.78 0.8 0.87 0.88 0.86 0.83 0.76 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 0 0 0.07 0 0 0 0 0 0 0.05 0.07 0.06 0.05 0.07 0.08 0.1 0.15 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1
Bud 0 0 0 0 1 0 0 1 0 0 3 0 1 0 2 2 1 0 2 3 0 3 2 6
Bud Neck 0 0 0 0 0 0 0 1 0 0 1 2 0 0 0 0 0 1 0 0 0 0 1 2
Bud Site 0 0 0 0 1 2 5 3 2 1 7 0 1 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 1 0 3 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 0 0
Cytoplasm 12 5 7 10 8 15 12 11 10 5 6 6 21 39 54 57 66 54 1 0 1 0 1 1
Endoplasmic Reticulum 1 0 0 0 0 0 1 1 0 0 0 1 1 1 0 16 26 28 0 0 0 0 1 1
Endosome 0 0 0 0 1 2 0 2 0 0 0 0 0 1 1 2 9 4 4 0 0 0 1 5
Golgi 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 1 2
Mitochondria 35 34 7 3 3 12 37 27 20 51 62 24 26 13 6 38 29 58 0 1 0 1 1 4
Nucleus 262 187 91 78 83 64 92 76 45 53 85 60 325 382 316 337 421 369 251 181 83 263 240 236
Nuclear Periphery 0 0 0 0 1 2 0 1 0 0 2 0 0 5 2 0 0 3 0 0 0 0 0 1
Nucleolus 1 0 1 0 0 0 3 2 0 1 4 2 1 6 6 0 3 0 2 0 0 5 8 20
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 1 4 2 3 5 1 1 0 2 0 1 2 5 8 4 5 0 0 2 3 4
Vac/Vac Membrane 2 4 6 0 3 1 0 0 4 0 5 2 3 2 3 22 37 26 14 14 7 31 48 44
Unique Cell Count 282 203 101 90 98 88 107 100 60 78 116 71 355 428 374 418 538 461 288 207 97 317 316 337
Labelled Cell Count 314 230 113 93 106 104 154 130 82 112 175 99 380 450 392 481 602 550 288 207 97 317 316 337


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.2 7.8 6.8 6.1 8.9 4.5 4.8 5.3 3.9 4.5 4.1 5.5 5.6 5.7 6.5 6.6 6.1 5.3 5.5 6.0
Std Deviation (1e-4) 1.1 0.9 5.7 3.8 2.0 9.4 1.4 1.8 4.5 1.5 3.0 1.0 1.1 1.4 1.6 1.3 1.3 1.3 1.3 1.3 1.9
Intensity Change (Log2) – – – -0.19 -0.34 0.19 -0.8 -0.71 -0.56 -1.0 -0.8 -0.94 -0.5 -0.49 -0.46 -0.27 -0.24 -0.35 -0.54 -0.49 -0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.3 2.2 1.1 1.0 1.9 -0.1 -0.5 0.4 -0.4 0.7 2.0 1.8 1.5 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.5 0 0 4.3 0 0 0 0.1 0 0 0.7 -0.6 1.6
Nucleus -0.7 -1.2 -3.1 -0.9 -2.7 -2.6 -3.7 -3.2 -1.1 0.5 -0.2 -1.4 -2.2 -2.7 -2.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 -0.5 0 0 0 0 -0.3 0.3 -0.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0822 2.8267 2.6138 2.6299 1.9534 2.6408 1.542 1.428 1.0152 0.8202 0.4036 0.8465 1.1943 1.2141 1.1311 1.5153 0.8654 1.066
Actin 0.0046 0 0.0045 0.0002 0.0257 0.0001 0.0194 0 0.0051 0.0011 0 0 0.0064 0 0.0059 0.0009 0 0.0004
Bud 0.0001 0 0.001 0.0003 0.0001 0 0.0019 0.0001 0.0002 0.0002 0.0001 0 0.0007 0 0.0006 0.0007 0 0.0002
Bud Neck 0.0008 0.0086 0.0007 0.0001 0.0016 0.0015 0.0032 0.0002 0.0048 0.0001 0.0007 0.0008 0.0052 0.0001 0.0013 0.0003 0.0003 0.001
Bud Periphery 0.0003 0.0001 0.0029 0.0004 0.0002 0 0.0065 0.0001 0.0003 0.0003 0.0001 0 0.0011 0 0.0005 0.0022 0 0.0009
Bud Site 0.0009 0.0095 0.0025 0.001 0.0006 0 0.0155 0.0004 0.0004 0.0007 0.0001 0.0001 0.0026 0 0.0016 0.0008 0 0.0001
Cell Periphery 0.0002 0.0003 0.0001 0.0001 0.0002 0 0.0009 0 0.0001 0 0 0 0.0003 0 0.0001 0.0004 0 0
Cytoplasm 0.0067 0.0001 0.0012 0.0002 0.0007 0.0044 0.0011 0.0011 0.0025 0.0001 0.001 0.0002 0.0132 0.0001 0.013 0.0412 0.0002 0.0019
Cytoplasmic Foci 0.0044 0 0.0034 0.0021 0.0082 0.0016 0.0108 0.0017 0.0009 0.001 0 0.0003 0.0161 0.0001 0.007 0.0045 0 0.0023
Eisosomes 0.0006 0 0.0001 0 0.0019 0.0001 0.0001 0 0 0.0001 0 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0016 0 0.0006 0.0001 0.0017 0.0004 0.0028 0.0001 0.0006 0 0.0001 0 0.0109 0 0.0023 0.0011 0 0.0003
Endosome 0.0119 0 0.0054 0.0107 0.0094 0.0007 0.0259 0.004 0.0033 0.0011 0.0001 0.0057 0.0191 0 0.0096 0.0101 0 0.0043
Golgi 0.0043 0 0.003 0.0045 0.0062 0.0001 0.0158 0.0004 0.0044 0.0012 0 0.0017 0.0036 0 0.0036 0.0011 0 0.0003
Lipid Particles 0.0141 0 0.0056 0.0022 0.0064 0.006 0.0299 0.0001 0.0016 0.0029 0 0.0001 0.0196 0 0.0021 0.0052 0 0.0002
Mitochondria 0.0103 0.0001 0.0054 0.0378 0.0188 0.0002 0.0127 0.0003 0.0144 0.0259 0.0003 0.0004 0.0069 0.0001 0.0053 0.0349 0 0.0026
None 0.0319 0 0.0223 0.0003 0.0038 0.018 0.0005 0.0001 0.0155 0.0001 0.0001 0.0001 0.0095 0 0.0054 0.0011 0 0.0005
Nuclear Periphery 0.0106 0.0007 0.0031 0.0011 0.0104 0.0013 0.0105 0.0037 0.002 0.0006 0.005 0.0009 0.026 0.0008 0.0057 0.0025 0.0014 0.0035
Nucleolus 0.0097 0.0069 0.0073 0.0025 0.01 0.0191 0.012 0.0068 0.0052 0.004 0.0232 0.0181 0.0114 0.008 0.0059 0.0087 0.0271 0.0136
Nucleus 0.867 0.9721 0.9234 0.9291 0.8724 0.9354 0.8039 0.9767 0.9343 0.9554 0.9617 0.9697 0.7717 0.9092 0.8748 0.8186 0.9245 0.9278
Peroxisomes 0.0069 0 0.0043 0.0028 0.016 0.0007 0.0063 0.0001 0.0006 0.0046 0 0 0.0019 0 0.0014 0.001 0 0.0002
Punctate Nuclear 0.0109 0.0015 0.0018 0.0007 0.0046 0.0098 0.0074 0.0026 0.0024 0.0002 0.0065 0.0015 0.0651 0.0815 0.052 0.0482 0.0463 0.0383
Vacuole 0.0013 0.0001 0.0008 0.0026 0.0004 0.0004 0.0097 0.0012 0.0009 0.0002 0.0006 0.0002 0.0052 0 0.0008 0.0086 0 0.0006
Vacuole Periphery 0.0009 0 0.0004 0.0011 0.0007 0 0.0032 0.0002 0.0003 0.0002 0.0002 0.0002 0.0031 0 0.0008 0.0079 0 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.3467 11.3251 13.8254 16.2098 13.2464 12.2533 14.5905 15.8309 14.5752 18.3375
Translational Efficiency 0.7078 0.6922 0.7084 0.6657 0.8847 0.8107 0.5979 0.5503 0.7093 0.6227

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Splicing factor; component of the U4/U6-U5 snRNP complex
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Prp6

Prp6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available