Standard name
Human Ortholog
Description Putative glycoside hydrolase of the mitochondrial intermembrane space
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Cytoplasm 0.93 0.95 0.95 0.98 0.97 0.91 0.85 0.86 0.81 0.76 0.68 0.77 0.99 0.99 1.0 0.97 0.97 0.93 0.8 0.87 0.81 0.83 0.81 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.09 0.05 0.1 0.05 0.22 0.27 0.23 0.32 0.36 0.49 0.42 0 0 0 0 0 0 0.07 0.07 0.09 0.06 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 1 3 0 0 0 0 0 0 0 2 0 4 2 4 5
Bud 1 0 4 1 2 2 3 8 5 3 10 12 1 1 0 0 0 0 0 0 0 8 11 19
Bud Neck 0 0 1 0 4 1 1 6 0 0 0 2 0 0 0 0 0 0 1 1 1 1 2 4
Bud Site 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 0 1 2 5 2 2 8 4 8 1 1 0 17 4 8 0 0 2 1 3 6
Cytoplasm 173 227 179 157 251 225 356 374 244 316 203 280 356 622 691 277 204 139 150 205 160 292 405 341
Endoplasmic Reticulum 1 1 2 0 1 2 3 0 3 4 6 1 2 1 0 18 10 4 0 1 0 2 1 2
Endosome 1 0 0 0 0 0 0 2 0 0 0 0 0 1 1 1 2 1 4 1 1 5 3 5
Golgi 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 4 0 0 0 4 0
Mitochondria 24 22 9 16 12 53 112 100 95 152 145 155 4 2 1 12 5 2 12 16 18 20 33 29
Nucleus 0 0 0 0 0 1 0 2 1 2 4 2 2 2 1 3 1 5 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 1 1 0 4 1 0 0 0 0 0 0 1 0 0 0 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 1 0 1 0 0 0 4 3 0 1 1 1 1 0 0 0 0 0 1 0 0 5 10 7
Vac/Vac Membrane 0 0 0 0 0 0 8 20 10 11 5 10 1 1 1 9 2 4 2 2 3 3 6 17
Unique Cell Count 186 239 188 161 258 246 418 437 300 418 298 366 361 628 694 286 210 149 188 238 197 352 500 453
Labelled Cell Count 205 251 198 174 272 286 493 519 361 502 383 472 368 631 695 337 228 164 188 238 197 352 500 453


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.2 3.3 3.3 3.4 3.4 3.0 3.4 3.7 3.7 4.1 4.1 4.1 4.0 4.0 5.9 6.9 7.3 4.1 4.0 4.5
Std Deviation (1e-4) 1.0 0.7 0.7 1.2 1.0 1.4 1.1 1.1 1.3 1.5 1.5 1.6 1.4 1.4 1.3 1.7 1.8 1.4 1.7 1.6 1.8
Intensity Change (Log2) – – – 0.02 0.07 0.04 -0.13 0.05 0.17 0.18 0.34 0.32 0.33 0.29 0.3 0.86 1.07 1.17 0.32 0.28 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7331 3.7528 3.4143 3.2264 2.8546 3.2938 -0.2545 -0.1812 -0.3949 -0.2031 -0.109 -0.3506 0.463 0.1754 0.3683 0.6139 0.2912 0.2883
Actin 0.0007 0.0022 0.001 0.0008 0.0009 0.0009 0.0805 0.0003 0.0116 0.0505 0.0476 0.0137 0.0597 0.0052 0.0097 0.0368 0.111 0.0003
Bud 0.0004 0.0054 0.0013 0.0004 0.0001 0.0001 0.0028 0.0002 0.0005 0.001 0.0006 0.0008 0.0013 0.0005 0.0025 0.0007 0.0014 0.0064
Bud Neck 0.0011 0.0005 0.0051 0.0004 0.0005 0.0023 0.0174 0.0003 0.0013 0.0005 0.0006 0.0008 0.0011 0.0015 0.0001 0.0016 0.0007 0.0002
Bud Periphery 0.0007 0.0049 0.0013 0.0004 0.0002 0.0003 0.0048 0.0001 0.0014 0.0025 0.0008 0.0023 0.0029 0.0008 0.0024 0.0008 0.0046 0.0041
Bud Site 0.008 0.0161 0.0049 0.0009 0.0001 0.0005 0.0184 0.0222 0.0008 0.002 0.0034 0.0004 0.0125 0.0017 0.0021 0.0074 0.0009 0.0002
Cell Periphery 0.0005 0.0007 0.0004 0.0001 0.0001 0.0001 0.0008 0.0002 0.0002 0.0003 0.0003 0.0001 0.0009 0.0005 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.2036 0.4062 0.3283 0.3575 0.3792 0.34 0.047 0.0854 0.1258 0.0809 0.0691 0.1031 0.1526 0.2587 0.1581 0.2241 0.1347 0.2371
Cytoplasmic Foci 0.0053 0.0066 0.0067 0.0091 0.0067 0.0083 0.0801 0.0159 0.0055 0.0061 0.0297 0.011 0.0548 0.0136 0.0052 0.0287 0.0216 0.018
Eisosomes 0.0002 0.0002 0.0002 0 0.0001 0.0001 0.0005 0.0003 0.0004 0.0004 0.0008 0.0002 0.0006 0.0002 0.0002 0.0003 0.0004 0
Endoplasmic Reticulum 0.0028 0.0022 0.0018 0.0013 0.002 0.0013 0.0009 0.0012 0.0018 0.0017 0.0019 0.0004 0.008 0.0023 0.0025 0.0008 0.0018 0.0009
Endosome 0.0016 0.0019 0.0016 0.0088 0.0012 0.0017 0.0075 0.0009 0.0016 0.0069 0.017 0.006 0.0206 0.0199 0.0034 0.0059 0.0518 0.0088
Golgi 0.0002 0.0002 0.0002 0.0006 0.0002 0.0002 0.0054 0.0002 0.0008 0.0142 0.011 0.0051 0.0196 0.0015 0.0006 0.0106 0.0162 0.0004
Lipid Particles 0.0005 0.0003 0.0002 0.0011 0.0003 0.0003 0.0102 0.0009 0.0013 0.0018 0.0163 0.0056 0.0557 0.0122 0.0005 0.0062 0.0121 0.001
Mitochondria 0.0003 0.0004 0.0004 0.0004 0.0002 0.0002 0.0018 0.0005 0.0008 0.0042 0.0038 0.0064 0.0088 0.0017 0.0009 0.0085 0.0042 0.0008
None 0.7679 0.5462 0.6424 0.6112 0.6043 0.6407 0.6555 0.8679 0.8394 0.8238 0.733 0.8374 0.5511 0.6433 0.7874 0.6148 0.5753 0.716
Nuclear Periphery 0.0006 0.0006 0.0004 0.0009 0.0005 0.0004 0.0003 0.0005 0.0012 0.0006 0.0013 0.0005 0.0021 0.0006 0.0051 0.0005 0.0018 0.0003
Nucleolus 0.0004 0.0003 0.0003 0.0003 0.0002 0.0001 0.0009 0.0002 0.0004 0.0001 0.0021 0.0001 0.0039 0.0003 0.0004 0.0002 0.0001 0.0002
Nucleus 0.0016 0.0021 0.0013 0.0014 0.0008 0.0007 0.0007 0.0003 0.0005 0.0003 0.0015 0.0002 0.001 0.0008 0.0047 0.0003 0.0004 0.001
Peroxisomes 0.0002 0.0002 0.0002 0.0004 0.0007 0.0002 0.0507 0.0008 0.0017 0.0008 0.0506 0.0044 0.0268 0.0249 0.0099 0.0365 0.0477 0.0006
Punctate Nuclear 0.0015 0.0011 0.0006 0.0023 0.0013 0.0009 0.0027 0.0008 0.0018 0.0005 0.0063 0.0013 0.005 0.0007 0.0023 0.0144 0.0015 0.0025
Vacuole 0.0019 0.0014 0.0013 0.0013 0.0004 0.0005 0.0107 0.0007 0.0009 0.0007 0.0019 0.0002 0.0093 0.0087 0.0013 0.0006 0.0097 0.0009
Vacuole Periphery 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001 0.0002 0.0002 0.0004 0.0001 0.0018 0.0003 0.0003 0.0002 0.002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 41.396 11.1344 18.4684 39.692 25.6484 26.3616 29.4694 34.1927 46.7789 36.5375
Translational Efficiency 1.23 2.2118 1.5422 1.2194 1.404 2.1272 1.1436 1.093 1.1905 1.2438

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative glycoside hydrolase of the mitochondrial intermembrane space
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Mrx18

Mrx18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available