Standard name
Human Ortholog
Description Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0.06 0.07 0.06 0.07 0.06 0.07 0.06 0.12 0.18 0.23 0.06 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0 0 0.17 0.26 0.13 0.37 0.35 0.62 0.43 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.92 0.9 0.92 0.89 0.83 0.81 0.73 0.82 0.69 0.73 0.63 0.68 0.87 0.76 0.73 0.83 0.81 0.79 0.81 0.82 0.82 0.79 0.74 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0.05 0.06 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.05 0.06 0 0.05 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.05 0.05 0.06 0 0.08 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 2
Bud 1 0 0 0 1 0 4 8 6 2 2 5 0 0 0 2 0 0 3 2 0 0 0 1
Bud Neck 0 0 1 0 3 3 5 9 2 5 2 2 1 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 3 3 6 2 6 12 11 7 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 16 15 10 23 24 35 32 32 25 29 20 29 43 61 25 26 24 2 2 2 1 3 4
Endoplasmic Reticulum 2 1 0 0 1 0 1 1 0 0 0 0 0 0 0 9 14 6 0 1 1 0 0 0
Endosome 0 3 0 4 8 6 5 2 3 4 0 1 0 6 7 8 6 11 2 8 3 1 3 11
Golgi 1 2 1 0 0 1 0 0 1 0 0 0 1 0 0 2 2 1 5 3 1 0 0 0
Mitochondria 62 6 3 10 7 63 132 74 162 146 259 141 0 0 1 18 9 7 5 4 2 2 2 7
Nucleus 366 379 345 279 316 306 366 455 303 304 262 222 208 183 196 328 315 276 350 377 323 63 128 126
Nuclear Periphery 0 2 0 1 2 0 5 1 2 1 1 2 0 0 2 2 5 0 0 1 0 0 0 2
Nucleolus 13 5 0 6 7 7 4 7 4 5 4 3 2 6 3 4 11 4 23 24 23 4 10 28
Peroxisomes 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0
SpindlePole 2 2 17 16 24 12 23 28 21 25 5 6 10 4 5 10 6 6 8 6 5 0 3 5
Vac/Vac Membrane 4 15 10 3 13 10 12 9 16 13 8 13 0 7 6 17 29 35 19 25 22 3 14 32
Unique Cell Count 397 423 377 314 380 377 502 556 437 417 415 327 240 240 269 395 388 351 431 461 395 81 175 230
Labelled Cell Count 459 431 392 330 410 435 598 628 559 542 584 422 251 249 282 425 423 370 431 461 395 81 175 230


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.3 6.3 5.8 5.7 5.0 5.0 5.1 4.4 4.4 3.9 4.1 6.3 6.7 6.3 7.1 7.6 7.2 5.5 6.8 7.0
Std Deviation (1e-4) 0.8 1.0 1.1 1.1 1.3 1.3 1.4 1.2 1.3 0.7 1.2 0.8 1.3 1.7 1.6 1.3 1.6 1.6 1.0 1.4 1.4
Intensity Change (Log2) – – – -0.11 -0.14 -0.34 -0.34 -0.3 -0.51 -0.54 -0.68 -0.61 0.01 0.08 -0.01 0.18 0.27 0.19 -0.2 0.11 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.6 1.3 1.5 1.9 1.2 2.0 1.3 1.8 1.3 3.8 5.8 7.3 1.5 1.7 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.7 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.3 0 7.7 10.4 6.8 0 0 0 0 0 0 0 3.2 1.7 0
Nucleus -1.2 -3.5 -4.1 -6.9 -4.1 -7.8 -6.8 -9.4 -7.9 -1.9 -5.3 -6.3 -3.5 -4.1 -4.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.4 1.1 -0.9 0.1 0.4 0 0 0 0 -0.2 -1.9 -1.8 -1.5 -2.4 -2.2
Vacuole -1.6 0.6 0 -0.2 -1.1 0.8 0.4 -0.7 1.0 0 0.2 -0.3 1.2 3.0 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2665 3.1769 2.6876 2.5954 2.766 2.8158 1.2422 1.3611 1.1731 1.0805 1.1889 1.1795 2.4271 2.0554 2.0615 2.501 2.3524 1.8508
Actin 0.0215 0 0.0004 0 0.0015 0.0028 0.0086 0.0001 0.0048 0.0002 0.0025 0.0033 0.0345 0 0.0055 0.001 0.0007 0.0057
Bud 0.0004 0 0 0 0.0001 0.0003 0.0004 0 0.0001 0.0001 0.0005 0.0001 0.0002 0 0 0.0002 0.0001 0.0002
Bud Neck 0.004 0.0001 0.0003 0.0001 0.0008 0.002 0.0013 0.0003 0.0003 0.0003 0.0009 0.0012 0.0025 0.0002 0.0002 0.0008 0.0016 0.0028
Bud Periphery 0.0013 0 0.0001 0 0.0001 0.0011 0.001 0.0001 0.0001 0.0001 0.0016 0.0003 0.0005 0 0.0001 0.0015 0.0004 0.0003
Bud Site 0.0024 0.0001 0.0001 0 0.0004 0.0003 0.001 0.0003 0.0012 0.0008 0.0004 0.0002 0.001 0.0002 0.0002 0.0006 0.0005 0.0007
Cell Periphery 0.0002 0 0.0001 0 0 0.0001 0.0003 0 0 0 0.0001 0 0.0001 0 0 0.0008 0.0001 0
Cytoplasm 0.0019 0.0003 0.0004 0 0.0039 0.0005 0.0037 0.0015 0.0005 0.0113 0.0006 0.0003 0.003 0.0031 0.0008 0.0005 0.0014 0.0023
Cytoplasmic Foci 0.003 0 0.0001 0 0.0009 0.0014 0.023 0.0001 0.0084 0.0053 0.0007 0.0012 0.0066 0.0001 0.0024 0.0002 0.0002 0.0021
Eisosomes 0.0002 0 0 0 0.0001 0.0001 0.0002 0 0 0 0 0 0.0003 0 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.0033 0 0.0014 0 0.0013 0.0005 0.0086 0.0005 0.0003 0.0005 0.0021 0.0004 0.0019 0.0013 0.0005 0.0014 0.0007 0.0003
Endosome 0.0096 0 0.0025 0 0.0087 0.0025 0.0209 0.0007 0.0094 0.0183 0.0154 0.0063 0.0083 0.0008 0.0019 0.0028 0.0014 0.0021
Golgi 0.0045 0 0.0002 0 0.0021 0.0016 0.0208 0 0.0067 0.0005 0.0049 0.0022 0.0022 0 0.0009 0.0007 0.0001 0.0022
Lipid Particles 0.008 0 0.0003 0 0.0104 0.0011 0.0245 0.0002 0.0128 0.0004 0.0023 0.003 0.0042 0.0002 0.0016 0.0061 0.0013 0.0056
Mitochondria 0.0038 0.0001 0.0014 0.0001 0.0147 0.0017 0.0064 0.0003 0.0112 0.0011 0.0045 0.0016 0.002 0.0002 0.0004 0.0109 0.0023 0.0007
None 0.0012 0 0.0003 0 0.0041 0.0013 0.0052 0.001 0.0003 0.0019 0.0003 0.0001 0.0028 0.0028 0.0012 0.0007 0.0011 0.0056
Nuclear Periphery 0.0273 0.0011 0.0083 0.0005 0.008 0.0045 0.017 0.0197 0.002 0.0133 0.0265 0.0011 0.0098 0.0073 0.0047 0.0553 0.0324 0.0022
Nucleolus 0.0302 0.0174 0.0144 0.0105 0.0726 0.07 0.0157 0.0208 0.0147 0.0184 0.0538 0.0549 0.0183 0.0258 0.014 0.0196 0.0583 0.0513
Nucleus 0.8615 0.9725 0.9656 0.988 0.8475 0.8941 0.8166 0.947 0.9188 0.9153 0.8643 0.9166 0.8701 0.9517 0.9591 0.8885 0.8821 0.8908
Peroxisomes 0.0039 0 0 0 0.0009 0.0014 0.0134 0 0.0035 0.0002 0.0002 0.0034 0.0112 0 0.0033 0.0004 0.0003 0.0026
Punctate Nuclear 0.0087 0.0082 0.0018 0.0008 0.0188 0.0112 0.0076 0.0069 0.0041 0.0078 0.0121 0.0031 0.0189 0.005 0.0028 0.0021 0.0135 0.0222
Vacuole 0.0016 0 0.0017 0 0.002 0.0009 0.0017 0.0002 0.0004 0.0035 0.0032 0.0003 0.001 0.0011 0.0002 0.0031 0.0008 0.0002
Vacuole Periphery 0.0017 0 0.0007 0 0.0009 0.0005 0.002 0.0003 0.0003 0.0008 0.0031 0.0002 0.0005 0.0002 0.0001 0.0025 0.0007 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8411 15.3542 14.9294 17.9319 16.51 15.9825 19.6735 19.6916 15.3711 21.8213
Translational Efficiency 0.8464 0.8539 0.8209 0.6607 0.8437 0.9021 0.6501 0.6776 0.8798 0.6932

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
Localization
Cell Percentages nucleus (69%), spindle pole (27%), mixed (2%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Orc2

Orc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Orc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available