Standard name
Human Ortholog
Description Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.29 0.32 0.81 0.64 0.59 0.54 0.46 – 0.41 0.43 0.41 0.41 0.64 0.78 0.73 0.89 0.83 0.77 0.18 0.22 0.46 0.24 0.33 0.27
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0 0.08 0 0 0 0 0 0
Endosome 0.13 0 0 0.11 0.08 0 0.08 – 0 0 0 0 0.1 0 0.09 0 0 0 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.19 0 0.06 0.06 0.49 0.24 – 0.47 0.36 0.48 0.46 0 0 0 0 0 0 0 0.05 0.08 0.05 0.06 0.07
Nucleus 0.29 0.29 0 0.09 0.09 0.06 0.15 – 0.15 0.16 0.11 0.12 0.09 0.05 0 0 0 0 0.35 0.34 0.12 0.27 0.14 0.16
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.06 – 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.06 0.09 0.1 0.1
Vac/Vac Membrane 0.3 0.27 0.1 0.19 0.25 0 0.14 – 0.11 0.11 0.1 0.1 0.27 0.17 0.31 0.16 0.26 0.22 0.2 0.23 0.18 0.2 0.19 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 2 1 3 0 6 2
Bud 0 0 0 0 0 4 0 – 4 4 6 8 0 0 0 0 0 2 6 3 3 4 12 18
Bud Neck 0 0 5 0 3 2 6 – 5 2 4 2 1 1 0 0 0 0 2 1 1 2 6 12
Bud Site 1 0 0 0 0 3 0 – 3 1 5 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 – 0 0 0 1 1 0 1 1 0 1 0 0 0 0 0 0
Cytoplasm 86 84 155 141 157 157 151 – 189 159 188 185 192 301 249 92 72 80 72 82 131 53 130 87
Endoplasmic Reticulum 1 2 2 0 0 0 0 – 1 3 1 0 1 0 0 7 1 8 0 0 2 2 4 3
Endosome 38 9 7 25 22 2 27 – 6 8 12 10 31 14 32 4 0 3 23 9 4 7 16 12
Golgi 1 0 0 0 0 0 0 – 0 0 0 0 1 1 1 0 0 0 5 1 1 2 3 1
Mitochondria 33 49 1 14 15 144 78 – 217 133 219 210 4 6 1 1 1 3 12 17 22 10 22 22
Nucleus 88 76 7 19 24 17 51 – 70 59 52 57 27 18 12 1 0 1 139 125 34 60 54 52
Nuclear Periphery 0 0 0 0 0 1 2 – 0 0 0 2 0 0 0 1 0 0 1 0 0 0 2 1
Nucleolus 1 0 0 0 0 2 0 – 0 1 1 0 0 0 0 1 0 0 7 6 1 4 5 8
Peroxisomes 0 0 0 0 0 0 1 – 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 3 5 2 5 7 3 20 – 6 7 8 13 0 2 1 0 0 0 37 24 15 21 41 30
Vac/Vac Membrane 90 69 19 42 67 12 46 – 52 41 44 46 81 64 105 16 23 23 79 85 50 45 75 53
Unique Cell Count 299 259 191 222 265 291 330 461 366 456 456 299 384 340 103 87 104 404 371 287 225 397 321
Labelled Cell Count 342 294 199 246 295 347 382 553 419 540 535 339 408 402 124 97 121 404 371 287 225 397 321


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 4.0 4.9 4.8 5.1 3.6 4.3 – 3.6 3.9 3.7 3.9 5.1 4.6 5.6 5.7 6.0 5.5 4.4 4.7 5.1
Std Deviation (1e-4) 0.8 0.6 0.7 1.3 1.3 1.5 1.2 – 0.6 0.8 0.7 0.7 1.3 1.3 2.3 1.5 0.9 1.3 1.1 1.6 2.0
Intensity Change (Log2) – – – -0.02 0.06 -0.45 -0.21 – -0.46 -0.32 -0.4 -0.34 0.05 -0.1 0.2 0.22 0.29 0.17 -0.16 -0.05 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.4 3.6 2.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9144 -0.5731 -0.8279 -0.8957 -0.9633 -0.6042 0.6404 0.5375 0.7686 -0.2443 0.4014 1.2574 2.8801 4.0503 3.5203 2.8474 4.343 3.6657
Actin 0.005 0.0004 0.0005 0.0023 0.008 0.001 0.0368 0.0002 0.0055 0.0003 0.001 0.0109 0.0096 0.0003 0.0101 0.0001 0.0006 0.0004
Bud 0.0031 0.0076 0.0028 0.0026 0.0065 0.0049 0.0005 0.0003 0.001 0.0001 0.0001 0.0005 0.0004 0.0008 0.0003 0.0001 0.0001 0.0002
Bud Neck 0.0055 0.0009 0.0009 0.0008 0.0037 0.0061 0.0008 0.0003 0.0009 0.0006 0.0025 0.0025 0.0009 0.0005 0.0007 0.0003 0.0003 0.0042
Bud Periphery 0.0013 0.0017 0.001 0.0018 0.0056 0.0018 0.0009 0.0003 0.0009 0.0001 0.0002 0.0008 0.0004 0.0009 0.0004 0 0.0001 0.0001
Bud Site 0.0061 0.0117 0.0051 0.0006 0.0035 0.0006 0.0028 0.0012 0.0068 0.0003 0.0006 0.0007 0.0012 0.0011 0.0009 0.0002 0.0002 0.0004
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0.0003 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.1511 0.2133 0.1637 0.1818 0.0851 0.1238 0.1962 0.2409 0.2559 0.2561 0.1913 0.1197 0.1861 0.2841 0.2605 0.3664 0.155 0.193
Cytoplasmic Foci 0.0362 0.0074 0.0174 0.0095 0.0259 0.0183 0.0158 0.0044 0.0116 0.0022 0.0057 0.0046 0.0074 0.0042 0.0065 0.0038 0.0033 0.0055
Eisosomes 0.0002 0.0001 0.0002 0 0.0004 0 0.0003 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0004 0 0.0001 0
Endoplasmic Reticulum 0.0063 0.0041 0.0049 0.0024 0.0032 0.0018 0.0059 0.0043 0.0062 0.0035 0.0062 0.0047 0.0055 0.0049 0.0045 0.0043 0.0032 0.0028
Endosome 0.018 0.0044 0.0056 0.0048 0.0115 0.0035 0.032 0.0029 0.0073 0.0018 0.0092 0.0197 0.0078 0.0035 0.0072 0.0051 0.0057 0.0035
Golgi 0.0021 0.0001 0.0002 0.0002 0.0019 0.0001 0.0126 0.0001 0.0009 0 0.0004 0.0102 0.0018 0.0001 0.0019 0 0.0001 0.0001
Lipid Particles 0.0062 0.0006 0.0013 0.0004 0.0072 0.0005 0.0085 0.0002 0.0006 0.0001 0.0012 0.0045 0.0058 0.0002 0.0025 0.0001 0.0001 0.0003
Mitochondria 0.0026 0.0014 0.0016 0.0018 0.0055 0.0005 0.0135 0.0005 0.0008 0.0005 0.0012 0.0176 0.0018 0.0009 0.001 0.0003 0.0006 0.0006
None 0.2238 0.3174 0.2686 0.2206 0.1364 0.1627 0.387 0.4783 0.4558 0.1799 0.3956 0.2295 0.3475 0.4301 0.4626 0.1296 0.5715 0.2896
Nuclear Periphery 0.0254 0.0199 0.0206 0.0251 0.0319 0.011 0.011 0.0081 0.0114 0.01 0.0353 0.0187 0.0224 0.0114 0.0068 0.013 0.0059 0.0083
Nucleolus 0.0121 0.0055 0.0066 0.0055 0.0192 0.0084 0.0022 0.0015 0.0016 0.002 0.0106 0.0058 0.0031 0.0022 0.0013 0.0012 0.0013 0.007
Nucleus 0.4052 0.3249 0.3904 0.4015 0.4195 0.5369 0.2313 0.2421 0.208 0.5168 0.2853 0.5293 0.3478 0.223 0.2112 0.4606 0.2446 0.4277
Peroxisomes 0.0062 0.0002 0.0003 0.0006 0.0015 0.0002 0.0036 0.0001 0.0004 0.0001 0.0003 0.0012 0.0036 0.0001 0.0011 0 0.0001 0.0001
Punctate Nuclear 0.0775 0.0747 0.1046 0.1358 0.2201 0.1163 0.028 0.0113 0.0195 0.0234 0.0484 0.0155 0.0389 0.0265 0.0161 0.012 0.0025 0.054
Vacuole 0.0048 0.0033 0.0027 0.0011 0.0016 0.0014 0.0073 0.0025 0.004 0.0019 0.0041 0.0022 0.0063 0.0043 0.0032 0.0026 0.0042 0.0019
Vacuole Periphery 0.0011 0.0005 0.0007 0.0006 0.0015 0.0003 0.0028 0.0003 0.0007 0.0002 0.0008 0.0013 0.0012 0.0006 0.0006 0.0004 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.1534 5.951 13.2477 22.6521 10.7889 6.6534 13.1623 23.8589 21.7285 13.9799
Translational Efficiency 0.7949 1.5778 1.1715 1.4908 0.9594 1.2729 1.0822 1.2145 1.5307 1.1962

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose
Localization
Cell Percentages nucleus (69%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Nrg2

Nrg2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nrg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available