Standard name
Human Ortholog
Description Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0.08 0.14 0.11 0.09 0.09 0.12 0.06 0.12 0.15 0.14 0.16 0.11 0.19 0.23 0.09 0.17 0.3 0 0 0 0 0 0
Cytoplasm 0.33 0.48 0.35 0.33 0.31 0.38 0.27 0.3 0.38 0.37 0.42 0.41 0.38 0.44 0.55 0.78 0.76 0.72 0.08 0.15 0.12 0.05 0.06 0.1
Endoplasmic Reticulum 0.67 0.52 0.58 0.54 0.57 0.44 0.53 0.57 0.52 0.49 0.47 0.43 0.63 0.54 0.46 0 0 0 0.79 0.69 0.75 0.82 0.79 0.76
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.07 0.12 0.18 0.15 0.17 0.14 0.06 0.09 0 0.07 0 0 0 0.12 0.12 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 2 5 3 12 6 6 1 6 4 9 4 4 3 2 4 2 8 6 5 17 24 22
Bud 3 1 1 4 6 20 9 15 12 14 14 24 0 3 5 1 2 3 13 15 4 20 23 23
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 1 2 6 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 15 17 26 26 37 29 70 40 51 79 68 98 44 127 155 26 47 79 0 0 3 7 20 22
Cytoplasm 82 97 66 76 127 125 153 208 161 201 201 255 146 296 375 213 213 192 22 31 24 24 39 69
Endoplasmic Reticulum 169 105 109 122 239 146 304 391 221 264 228 267 246 363 317 2 6 3 222 147 147 404 526 517
Endosome 0 0 0 1 0 0 1 0 0 0 0 0 1 0 1 4 1 1 0 1 1 1 0 2
Golgi 1 1 1 9 17 2 14 1 2 1 3 6 1 2 1 6 1 1 1 0 0 0 0 1
Mitochondria 0 2 0 0 0 6 0 0 2 2 1 2 1 1 0 5 5 0 0 3 1 2 5 3
Nucleus 0 0 0 0 0 0 0 4 1 0 0 3 0 0 0 2 1 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 1 5 3 1
Nucleolus 0 0 0 0 0 0 0 3 0 3 1 1 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 1 0 0 0
Vac/Vac Membrane 13 6 13 27 73 48 97 99 24 49 14 42 12 17 12 33 34 22 1 2 2 3 4 6
Unique Cell Count 251 202 189 228 416 330 575 691 424 538 482 616 388 671 685 274 280 267 282 215 198 495 664 684
Labelled Cell Count 283 232 219 271 502 388 654 767 475 619 534 707 456 815 870 295 314 304 282 215 198 495 664 684


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 42.0 24.6 20.8 33.5 35.7 30.2 41.7 44.7 45.2 49.3 51.5 52.9 38.1 35.6 33.1 22.3 22.6 28.4 35.7 37.5 36.9
Std Deviation (1e-4) 19.7 12.6 8.7 18.3 18.5 16.3 17.4 19.9 21.0 23.7 24.3 22.8 21.0 19.5 16.2 10.4 9.8 12.5 16.2 16.0 16.8
Intensity Change (Log2) – – – 0.69 0.78 0.54 1.0 1.1 1.12 1.25 1.31 1.35 0.87 0.78 0.67 0.1 0.12 0.45 0.78 0.85 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 1.7 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 3.1 0 0 0 0 0 2.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -1.8 -1.8 -0.6 -3.7 -0.6 0.3 0.1 0.7 -0.8 1.6 2.7 -1.4 0.9 4.0
Cytoplasm -0.3 -1.1 0.7 -2.2 -1.3 0.7 0.6 1.6 1.6 0.6 2.3 4.8 9.3 8.9 7.9
Endoplasmic Reticulum -0.9 -0.1 -2.9 -1.1 -0.3 -1.3 -2.0 -2.4 -3.5 1.3 -0.9 -2.8 -14.1 -13.7 -13.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.7 3.5 2.6 3.4 2.7 -0.6 0.9 -2.4 0 -2.1 -2.9 -3.7 1.8 1.9 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 37.479 36.3426 36.5898 30.5262 27.4998 37.7336 33.5028 48.6329 38.9323 30.4035 37.6606 37.9652 38.2081 38.4512 36.5155 37.5617 33.2436 36.8119
Actin 0.0107 0.0086 0.004 0.0012 0.0103 0.0077 0.0121 0.003 0.0041 0.0213 0.0031 0.0049 0.0082 0.0094 0.008 0.0049 0.027 0.005
Bud 0.0006 0.0042 0.0005 0.0004 0.0003 0.0013 0.0019 0.0059 0.0018 0.0057 0.0054 0.0004 0.0011 0.0033 0.004 0.0009 0.0108 0.0012
Bud Neck 0.0016 0.0012 0.0015 0.0013 0.0013 0.0038 0.0089 0.0011 0.0016 0.0049 0.0048 0.0063 0.0006 0.0007 0.0011 0.0019 0.0072 0.0114
Bud Periphery 0.0009 0.0035 0.0013 0.0021 0.0005 0.004 0.0062 0.0032 0.0024 0.0145 0.0068 0.0007 0.0008 0.0013 0.0067 0.0025 0.0146 0.0019
Bud Site 0.0038 0.0332 0.0029 0.0002 0.0004 0.0003 0.0115 0.0097 0.0076 0.0044 0.0007 0.0004 0.0034 0.0086 0.0058 0.0027 0.0027 0.0001
Cell Periphery 0.0103 0.0246 0.0151 0.0104 0.0016 0.005 0.0183 0.045 0.024 0.0196 0.0132 0.0031 0.0123 0.0142 0.0087 0.0141 0.0081 0.0024
Cytoplasm 0.1791 0.233 0.2325 0.1153 0.2208 0.2217 0.1884 0.2893 0.2509 0.2518 0.3838 0.2482 0.1507 0.3084 0.2592 0.2347 0.2508 0.263
Cytoplasmic Foci 0.0036 0.0016 0.0048 0.0184 0.0182 0.0016 0.0091 0.0024 0.0039 0.013 0.0018 0.0046 0.0072 0.0012 0.0019 0.0014 0.0017 0.001
Eisosomes 0.0042 0.0002 0.0002 0.0002 0.0034 0 0.0006 0.0001 0.0005 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.6192 0.5872 0.6288 0.7234 0.5534 0.6763 0.574 0.5447 0.5837 0.5701 0.4212 0.6277 0.6556 0.593 0.6185 0.6694 0.5713 0.6593
Endosome 0.009 0.001 0.0034 0.0017 0.0145 0.0011 0.0062 0.0019 0.0046 0.0012 0.0046 0.0046 0.0063 0.0009 0.0012 0.0011 0.0011 0.001
Golgi 0.0015 0.0004 0.0009 0.0004 0.0015 0.0005 0.0045 0.0007 0.0015 0.001 0.0027 0.0025 0.0016 0.0004 0.0004 0.0004 0.0004 0.0002
Lipid Particles 0.0074 0.0208 0.0105 0.0128 0.0098 0.0038 0.013 0.011 0.0172 0.013 0.0127 0.009 0.0077 0.0169 0.0045 0.0109 0.0094 0.0015
Mitochondria 0.0038 0.0002 0.0009 0.0032 0.0031 0.0002 0.007 0.0011 0.0173 0.005 0.0121 0.0076 0.0009 0.0001 0.0004 0 0.0002 0.0001
None 0.0095 0.0015 0.0079 0.0038 0.0281 0.0017 0.008 0.0011 0.0096 0.0041 0.0034 0.0047 0.0118 0.0007 0.0172 0.0017 0.0029 0.0017
Nuclear Periphery 0.1004 0.0565 0.0576 0.0614 0.0894 0.0545 0.0791 0.0584 0.0457 0.0339 0.0708 0.0525 0.1068 0.0301 0.0413 0.0404 0.0705 0.0365
Nucleolus 0.0021 0.0007 0.0006 0.0069 0.0053 0.0001 0.0035 0.0002 0.0006 0.0047 0.0061 0.001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0001
Nucleus 0.01 0.0075 0.0067 0.0044 0.0168 0.0082 0.0132 0.0045 0.0049 0.0144 0.0221 0.0096 0.0104 0.0031 0.0096 0.0042 0.01 0.0078
Peroxisomes 0.0004 0.0001 0.0012 0.0005 0.001 0 0.0124 0.0002 0.0015 0.0013 0.0002 0.0022 0.0014 0 0.0001 0 0.0002 0
Punctate Nuclear 0.0058 0.0019 0.0064 0.0064 0.0123 0.0009 0.0042 0.0008 0.0017 0.0018 0.0057 0.0025 0.0027 0.0005 0.0021 0.0026 0.0015 0.0007
Vacuole 0.0113 0.0114 0.0111 0.0211 0.0056 0.0066 0.0146 0.0152 0.0129 0.0121 0.0149 0.0066 0.0087 0.0068 0.008 0.0053 0.008 0.0042
Vacuole Periphery 0.0049 0.0008 0.0013 0.0044 0.0026 0.0006 0.0031 0.0006 0.002 0.002 0.0038 0.0007 0.0014 0.0002 0.0009 0.0004 0.0011 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 276.2869 88.8206 137.5989 182.0537 143.5132 594.08 97.2903 69.1204 99.3596 75.0657
Translational Efficiency 1.236 0.8862 1.1727 1.4161 1.5892 1.3913 1.0682 2.1813 1.991 1.8962

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
Localization
Cell Percentages ER (90%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Tip1

Tip1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tip1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available