Standard name
Human Ortholog
Description Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; essential for the integrity and function of EGO; gene exhibits synthetic genetic interaction with MSS4
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0.09 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.05 0 0.1 0.07 0.14 – 0.19 0.19 0.17 0.28 0.28 0 0.06 0 0.08 0.09 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.07 – 0 0.1 0.1 0.13 0.14 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.06 0.08 0.08 0.14 0.18
Golgi 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0.06 0.1 0.1 0 0 0 0 0 0
Mitochondria 0 0 0.35 0.33 0.31 0.39 – 0.45 0.52 0.52 0.51 0.44 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.05 0.05 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Nuclear Periphery 0 0.05 0.17 0.17 0.06 0.16 – 0.06 0.11 0.06 0.05 0.13 0.15 0.18 0.15 0.13 0.15 0.17 0 0 0.08 0 0.08 0
Nucleolus 0.07 0.06 0.14 0 0 0 – 0 0 0 0 0 0.09 0.05 0.06 0.08 0.05 0.1 0 0 0 0.05 0 0.06
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.86 0.85 0.72 0.7 0.72 0.51 – 0.45 0.38 0.33 0.22 0.23 0.87 0.86 0.88 0.85 0.8 0.89 0.79 0.84 0.72 0.75 0.63 0.61
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 2 1 3 1 0 0 0 1 0 0 0 1 0 0 0 0
Bud 0 0 3 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 5 1 0 0 0 1
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 1 0 1 – 4 3 3 12 11 0 0 2 5 0 0 0 0 0 0 0 0
Cytoplasm 22 24 1 4 8 20 – 37 22 16 38 37 2 12 5 12 12 3 3 1 0 0 1 0
Endoplasmic Reticulum 0 1 0 0 1 11 – 8 12 10 18 18 1 2 3 10 8 4 0 0 0 0 0 0
Endosome 9 8 0 0 1 2 – 1 0 0 0 0 4 9 8 4 3 2 27 30 6 10 21 28
Golgi 7 16 3 0 6 3 – 2 1 1 4 0 1 3 0 10 13 7 3 2 0 0 2 1
Mitochondria 12 12 24 13 35 58 – 88 61 50 69 59 4 2 4 3 6 3 5 5 2 3 4 2
Nucleus 25 21 4 0 2 1 – 2 1 2 0 0 2 2 1 4 2 0 30 9 2 2 5 6
Nuclear Periphery 19 24 12 7 7 24 – 12 13 6 7 17 19 33 29 20 19 12 11 12 6 5 13 5
Nucleolus 31 29 10 1 2 4 – 0 0 2 0 0 11 10 12 13 6 7 8 9 0 6 4 8
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 – 0 0 0 0 0 2 2 0 2 2 0 1 2 0 0 1 4
Vac/Vac Membrane 394 391 50 28 81 76 – 87 45 32 29 30 110 159 170 135 102 63 383 409 58 101 99 94
Unique Cell Count 456 459 69 40 113 148 194 117 96 134 133 127 185 193 159 127 71 484 489 81 135 158 155
Labelled Cell Count 520 527 108 54 143 200 241 161 123 181 173 156 234 234 219 173 101 484 489 81 135 158 155


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.3 15.9 8.0 9.9 10.4 9.3 – 8.8 8.2 8.1 8.0 7.9 12.6 13.3 13.7 17.8 17.0 17.0 14.2 14.4 14.4
Std Deviation (1e-4) 3.3 3.6 2.3 1.8 2.7 2.4 – 2.1 1.9 1.9 1.9 1.6 3.2 3.2 3.2 4.8 4.7 4.6 4.6 4.4 4.2
Intensity Change (Log2) – – – 0.3 0.37 0.21 – 0.13 0.03 0.02 -0.0 -0.02 0.65 0.73 0.76 1.15 1.08 1.08 0.82 0.84 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 2.8 – 3.6 3.5 3.2 4.6 4.5 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 3.2 3.2 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 1.4 0
Mitochondria -0.2 -0.5 0.6 – 1.5 2.3 2.2 2.3 1.3 -6.0 -7.9 -7.5 -7.1 -5.6 -4.6
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -2.4 -0.2 – -2.8 -1.2 -2.3 -2.8 -0.9 -0.4 0.1 -0.5 -1.0 -0.4 -0.1
Nucleolus 0 0 0 – 0 0 -3.0 0 0 -1.3 -2.4 -2.1 -1.5 -2.4 -0.8
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 -0.1 -2.9 – -3.9 -4.5 -5.0 -7.0 -6.9 2.4 2.5 3.0 2.2 1.3 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.7264 12.4438 10.0168 10.0374 9.9696 11.3878 8.1441 11.1474 10.0228 8.9603 8.4745 10.0778 9.7222 10.9078 9.8643 8.9156 9.6331 9.5494
Actin 0.0202 0 0.0211 0.0166 0.0095 0 0.0207 0 0.0048 0.0026 0.0027 0 0.0004 0.0008 0.0002 0.0001 0 0.0014
Bud 0.0008 0.0002 0.0014 0.0001 0.0003 0.0007 0.0005 0.0004 0.0009 0.0003 0.0017 0.0002 0.0013 0.0018 0.0018 0.0021 0.0006 0.0014
Bud Neck 0.0005 0.0003 0.0004 0.0001 0.0008 0.0004 0.0012 0.0002 0.0005 0.0001 0.0011 0.0004 0.0007 0.0004 0.0008 0.0005 0.0005 0.0013
Bud Periphery 0.0012 0.0002 0.0011 0.0001 0.0004 0.0005 0.0009 0.0003 0.0012 0.0003 0.0013 0.0002 0.0006 0.0006 0.0008 0.0006 0.0006 0.0006
Bud Site 0.0009 0.0003 0.0016 0.0003 0.0008 0.0001 0.002 0.0006 0.0019 0.0005 0.0035 0.0002 0.0019 0.0038 0.0017 0.0005 0.0002 0.0006
Cell Periphery 0.0008 0.0006 0.0004 0.0002 0.0004 0.0004 0.0009 0.0004 0.0007 0.0001 0.0007 0.0002 0.0004 0.0003 0.0004 0.0002 0.0002 0.0001
Cytoplasm 0.0153 0.0759 0.1156 0.1172 0.0262 0.0787 0.0382 0.0535 0.0616 0.0035 0.016 0.0446 0.2517 0.3293 0.3763 0.1145 0.1598 0.3046
Cytoplasmic Foci 0.0045 0.0057 0.0117 0.0097 0.0089 0.007 0.0108 0.0022 0.0037 0.0163 0.0066 0.0055 0.0084 0.0105 0.0147 0.0129 0.0034 0.0163
Eisosomes 0.0001 0 0.0002 0.0001 0.0001 0 0.0007 0 0 0 0.0001 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0016 0.0017 0.0029 0.0004 0.0012 0.001 0.0055 0.0016 0.0024 0.0009 0.0034 0.0016 0.003 0.0073 0.0037 0.0017 0.0085 0.0052
Endosome 0.0478 0.0293 0.0509 0.0421 0.0483 0.0292 0.0784 0.0328 0.0547 0.0864 0.0582 0.0573 0.0721 0.0492 0.0954 0.1353 0.0994 0.0929
Golgi 0.0057 0.0011 0.0062 0.0018 0.0064 0.0003 0.0062 0.0006 0.0032 0.0055 0.0024 0.0008 0.0008 0.0019 0.0014 0.0007 0.0004 0.001
Lipid Particles 0.0087 0.0078 0.0066 0.013 0.0196 0.0002 0.0113 0.0004 0.005 0.0206 0.009 0.0002 0.0005 0.0015 0.0017 0.0002 0.0001 0.0014
Mitochondria 0.0155 0.0006 0.0152 0.0026 0.0098 0.0004 0.0147 0.0011 0.0121 0.0021 0.009 0.0006 0.0006 0.0017 0.001 0.0002 0.0003 0.0011
None 0.0008 0.0001 0.0009 0.0002 0.0004 0.0011 0.0026 0.0001 0.0008 0.0005 0.0012 0.0001 0.0008 0.0006 0.0008 0.0007 0.0002 0.0011
Nuclear Periphery 0.021 0.0103 0.0196 0.0077 0.0113 0.015 0.0267 0.0178 0.0131 0.0014 0.009 0.0188 0.0326 0.027 0.0103 0.0069 0.018 0.0257
Nucleolus 0.0048 0.0076 0.0004 0.0002 0.0015 0.0116 0.0053 0.0005 0.0008 0.0002 0.0007 0.0004 0.0007 0.0005 0.0025 0.0002 0.0003 0.0009
Nucleus 0.0159 0.0055 0.0065 0.0015 0.0025 0.0075 0.011 0.0046 0.0055 0.0006 0.0037 0.0035 0.0463 0.0176 0.0157 0.0044 0.0215 0.0342
Peroxisomes 0.0017 0.0002 0.0083 0.048 0.011 0.0001 0.0045 0 0.0004 0.0241 0.0064 0.0002 0.0002 0.0008 0.0002 0.0002 0 0.0021
Punctate Nuclear 0.0056 0.0001 0.0007 0.0006 0.0006 0.0059 0.0056 0 0.0006 0.001 0.0038 0.0001 0.0005 0.0005 0.0005 0.0002 0.0001 0.0012
Vacuole 0.3614 0.3474 0.3259 0.3416 0.3343 0.3085 0.3936 0.4077 0.4371 0.38 0.3624 0.3299 0.4112 0.3697 0.336 0.5653 0.5072 0.3614
Vacuole Periphery 0.4653 0.5053 0.4025 0.3959 0.5057 0.5312 0.3587 0.4752 0.3891 0.453 0.497 0.5352 0.1654 0.1745 0.1341 0.1527 0.179 0.1455

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.6927 24.2056 25.4455 32.841 17.5535 31.5728 38.7508 30.9721 33.7714 38.7484
Translational Efficiency 1.4359 1.9692 1.2802 1.6774 2.7132 1.3759 1.4701 1.8325 1.7531 1.8243

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; essential for the integrity and function of EGO; gene exhibits synthetic genetic interaction with MSS4
Localization
Cell Percentages vacuolar membrane (95%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Slm4

Slm4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Slm4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available