Standard name
Human Ortholog
Description Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06
Bud 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.08 0 0 0 0 0 0
Golgi 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0 0.29 0.42 0.4 0 0.07 0 0.12 0.1 0.1
Mitochondria 0.87 0.95 0.92 0.93 0.9 0.9 0.89 0.91 0.97 0.97 0.95 0.96 0.94 0.91 0.95 0.06 0.12 0.08 0.88 0.74 0.82 0.65 0.66 0.66
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0.06 0 0 0.09 0.09 0.11 0.07 0.08 0.08 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0.07 0.06 0.08 0.06 0 0.06 0 0 0 0 0.08 0 0.09 0 0 0 0 0 0
Peroxisomes 0.09 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.13 0.09 0.05 0 0.06 0.06 0.07 0.12
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0.12 0.1 0 0.06 0.08 0.07 0 0 0 0 0 0.08 0.09 0.06 0.19 0.19 0.13 0 0.05 0 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 5 1 1 2 2 0 0 0 1 0 4 2 2 3 2 1 4 3 10 14 22
Bud 3 4 8 9 8 4 24 24 12 21 23 19 2 5 12 2 8 6 1 2 1 1 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 1 2 0 1 1 5 7 3 4 6 4 0 0 0 0 0 1
Cell Periphery 5 4 8 11 4 4 3 3 5 6 0 7 5 11 10 8 11 9 0 0 0 0 0 1
Cytoplasm 0 1 6 7 14 11 10 12 6 2 10 6 6 10 8 6 18 8 0 0 1 0 0 0
Endoplasmic Reticulum 0 0 1 3 0 4 2 3 4 4 4 4 1 5 5 2 5 7 0 0 0 1 0 0
Endosome 5 2 12 1 10 3 11 13 0 1 5 0 3 8 2 14 8 13 3 7 17 8 10 8
Golgi 23 0 4 3 4 3 0 0 0 0 0 0 8 55 11 55 80 64 7 11 10 25 32 36
Mitochondria 196 154 345 290 308 395 608 761 370 405 430 493 300 386 437 12 24 13 224 123 317 136 202 251
Nucleus 1 0 0 0 1 1 6 7 0 2 2 2 0 1 0 0 3 0 0 0 1 0 0 0
Nuclear Periphery 12 10 13 8 32 39 72 57 32 32 43 57 14 12 12 8 4 6 1 1 2 3 1 2
Nucleolus 15 3 11 11 15 32 42 65 23 18 26 20 7 3 9 15 7 15 1 0 0 0 3 1
Peroxisomes 21 3 23 7 15 1 28 13 0 0 3 5 10 9 10 39 25 15 12 5 21 13 21 44
SpindlePole 3 1 5 3 5 6 9 20 3 3 8 7 2 1 3 11 12 5 0 0 1 0 1 1
Vac/Vac Membrane 26 19 36 12 19 37 49 36 12 15 14 21 26 38 27 35 37 21 0 8 3 9 17 6
Unique Cell Count 226 162 373 311 344 439 682 833 382 416 455 513 320 425 460 188 192 160 255 167 388 211 309 382
Labelled Cell Count 310 203 475 370 437 542 871 1023 470 513 574 646 384 548 548 209 245 185 255 167 388 211 309 382


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 9.3 7.2 9.6 8.9 7.6 6.7 6.2 5.7 5.4 5.3 4.9 11.0 10.3 8.2 11.1 12.9 13.6 12.2 13.1 13.5
Std Deviation (1e-4) 3.0 2.5 2.6 4.1 2.2 1.9 1.7 1.4 1.7 1.2 1.3 1.2 2.3 2.1 1.8 3.0 3.9 4.1 3.1 3.3 3.2
Intensity Change (Log2) 0.41 0.29 0.07 -0.11 -0.22 -0.35 -0.41 -0.44 -0.55 0.6 0.51 0.18 0.62 0.83 0.91 0.75 0.86 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0.6 0.2 -1.5 1.2 0.7 0.9 2.2 2.2 1.3 0 -1.1 0.4 0 1.4 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.1 -1.0 -1.5 0 0 -0.9 -0.7 0 -0.9 -0.6 0.4 0 1.4 2.2 2.1
Cytoplasm 0.6 2.0 0.9 -0.2 -0.2 0 0 0.6 -0.6 0.3 0.7 0.1 0 4.3 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.8 -0.2 -2.7 -1.7 -1.9 -3.5 -3.3 -2.1 -4.1 -2.1 -1.2 -3.1 2.2 0.6 2.5
Golgi 0 0 0 0 0 0 0 0 0 1.4 6.4 1.4 10.3 12.8 12.3
Mitochondria 0.4 -1.4 -1.3 -1.8 -0.7 2.7 3.1 1.2 2.3 0.6 -0.8 1.5 -20.0 -18.9 -19.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.7 3.2 3.1 4.0 2.3 2.8 2.5 3.4 4.2 0.6 -0.5 -0.7 0.5 -0.9 0.2
Nucleolus 0.4 1.0 2.8 2.3 3.2 2.0 1.0 1.9 0.8 -0.6 -2.4 -0.9 2.7 0.4 3.2
Peroxisomes -2.5 -1.1 -5.0 -1.5 -4.3 -4.9 -5.1 -4.5 -4.4 -1.9 -2.9 -2.9 5.2 2.8 1.3
SpindlePole 0 0 0 0 1.2 0 0 0.5 0 0 0 0 3.0 3.2 0
Vacuole -3.0 -2.1 -0.6 -1.4 -3.6 -3.7 -3.4 -4.0 -3.3 -0.7 -0.3 -2.1 3.0 3.2 1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.3422 17.3687 18.2192 17.6221 17.062 17.1392 16.0361 17.5243 18.2574 15.7255 14.7206 16.6728 18.2463 18.3774 18.3855 19.7553 18.7336 17.899
Actin 0.0086 0.0049 0.0141 0.0013 0.0002 0.0004 0.0302 0.0043 0.0149 0.0163 0.0374 0.0005 0.0068 0.0028 0.001 0.0034 0.0004 0.0003
Bud 0.0002 0.0001 0.0001 0.0001 0 0 0.0003 0.0002 0.0001 0.0005 0.0044 0 0.0001 0.0016 0 0 0 0
Bud Neck 0.0002 0.0001 0 0.0001 0 0 0.0009 0.0001 0.0002 0.0015 0.0019 0 0.0011 0.0002 0 0 0 0
Bud Periphery 0.0003 0.0002 0.0002 0.0002 0.0001 0 0.0004 0.0003 0.0002 0.0014 0.0144 0 0.0002 0.0033 0.0001 0.0001 0 0
Bud Site 0.0011 0.0008 0.0001 0.0003 0 0 0.0014 0.0004 0.0004 0.0022 0.0052 0 0.0012 0.0167 0.0001 0 0 0
Cell Periphery 0.0002 0.0003 0.0001 0.0007 0.0002 0 0.0001 0.0002 0.0001 0.0002 0.0004 0 0.0002 0.0004 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.0036 0 0.0003 0.0002 0.0002 0.001 0.0043 0 0 0 0 0
Cytoplasmic Foci 0.0028 0.002 0.0003 0.0025 0.0001 0.0001 0.019 0.0012 0.0058 0.0088 0.0009 0.0002 0.0056 0.0005 0.0044 0 0.0003 0.0001
Eisosomes 0.0008 0.0002 0.0005 0.0004 0.0003 0.0001 0.0004 0.0003 0.0002 0.0003 0.0012 0.0001 0.0002 0.0004 0.0001 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0 0 0 0 0 0 0.0016 0 0.0001 0.0008 0.0005 0.0004 0.0025 0.0001 0 0 0 0
Endosome 0.0016 0.0008 0.0004 0.0012 0.0004 0.0005 0.0129 0.0027 0.001 0.0111 0.0026 0.0036 0.0103 0.0028 0.0006 0 0.0002 0.0008
Golgi 0.0033 0.0032 0.0029 0.0035 0.0004 0.004 0.0092 0.0084 0.0021 0.0122 0.0038 0.004 0.005 0.0023 0.0011 0.0002 0.0012 0.0071
Lipid Particles 0.0215 0.0242 0.0086 0.0503 0.0012 0.0033 0.0128 0.0103 0.0059 0.0253 0.0037 0.0102 0.0101 0.0137 0.0084 0.0009 0.0077 0.0018
Mitochondria 0.9422 0.9467 0.9655 0.9208 0.9905 0.9814 0.8653 0.9552 0.9535 0.8701 0.9151 0.9635 0.9297 0.9345 0.9754 0.9938 0.9687 0.9841
None 0 0 0 0 0 0 0.0039 0 0.0004 0.0003 0.0007 0.0002 0.0041 0.0005 0.0001 0 0 0
Nuclear Periphery 0.0001 0 0 0.0001 0 0.0021 0.0112 0 0.0001 0.0021 0.0009 0.0063 0.0023 0.0002 0 0 0 0
Nucleolus 0.0003 0.0027 0.0001 0.0005 0.0001 0 0.0006 0.0012 0.0004 0.0003 0.0005 0.0001 0.001 0.0008 0.0001 0 0 0
Nucleus 0.0001 0.0003 0 0.0001 0 0 0.0011 0.0001 0.0002 0.0003 0.0005 0.0015 0.0007 0.0003 0 0 0 0
Peroxisomes 0.0092 0.0058 0.0024 0.007 0.0003 0.0006 0.0098 0.0061 0.0065 0.0419 0.0034 0.0007 0.0028 0.0036 0.007 0.0007 0.0209 0.0012
Punctate Nuclear 0.0001 0.0001 0 0.0001 0 0 0.0066 0 0.0003 0.0005 0.0002 0.0001 0.0018 0.0001 0.0001 0 0 0
Vacuole 0.0009 0.0033 0.0004 0.001 0.0015 0.0003 0.0013 0.0016 0.0008 0.0013 0.0009 0.0007 0.0029 0.0018 0.0002 0 0 0.0003
Vacuole Periphery 0.0066 0.0039 0.004 0.0098 0.0047 0.0068 0.0071 0.0075 0.0066 0.0021 0.001 0.0068 0.0073 0.0131 0.0011 0.0003 0.0004 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 118.1412 105.4425 104.4905 96.3549 109.2014 63.6517 74.3395 71.8415 64.2612 99.3335
Translational Efficiency 0.8584 0.9815 0.7998 0.7501 0.7523 1.0636 0.7966 0.6525 0.6826 0.7346

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mis1

Mis1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mis1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available