Standard name
Human Ortholog
Description Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.63 0.56 0.55 0.6 0.51 0.53 0.36 0.28 0.38 0.3 0.33 0.31 0.53 0.4 0.35 0.37 0.41 0.38 0.15 0.24 0.16 0.12 0.1 0.1
Bud 0.11 0.19 0.1 0.15 0.11 0.08 0.08 0.06 0.08 0.06 0.07 0.08 0 0.07 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.54 0.44 0.53 0.46 0.32 0.34 0.29 0.19 0.34 0.25 0.27 0.25 0.73 0.72 0.83 0.58 0.57 0.7 0.75 0.53 0.75 0.79 0.79 0.79
Cytoplasm 0 0.09 0.05 0.06 0 0 0 0 0 0 0 0 0.07 0.1 0.08 0.14 0.1 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.06 0 0 0.08 0.14 0.09 0.15 0.1 0.1 0.09 0.08 0 0.05 0 0.05 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0
Mitochondria 0.51 0.51 0.64 0.74 0.71 0.78 0.79 0.76 0.85 0.88 0.89 0.87 0.09 0.06 0 0.1 0.07 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0 0.27 0.27 0.22 0.18 0.17 0.1 0.09 0.08 0.05 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 146 90 170 150 148 230 196 152 150 186 158 206 61 88 89 123 280 130 38 44 51 27 31 40
Bud 25 30 31 38 32 36 42 32 33 40 35 54 5 15 11 11 29 12 2 4 4 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 16 5 5 6 7
Bud Site 2 5 4 3 2 3 2 0 1 1 1 4 1 0 0 0 3 1 – – – – – –
Cell Periphery 126 71 162 116 92 150 154 102 136 159 128 166 85 158 209 191 394 241 190 98 237 188 247 327
Cytoplasm 6 15 15 16 11 12 17 9 9 5 12 8 8 21 20 47 72 33 0 2 1 0 1 2
Endoplasmic Reticulum 6 9 8 10 23 60 48 82 38 64 44 52 5 10 9 16 45 17 2 3 6 1 5 11
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 0 1 2 1 0 0 1
Golgi 0 0 0 1 3 1 5 19 1 4 2 5 2 4 1 0 31 30 0 0 0 0 2 2
Mitochondria 118 81 197 186 207 339 426 413 334 549 421 582 10 12 4 32 50 20 5 4 6 10 11 12
Nucleus 0 0 1 1 0 0 1 1 2 0 1 0 1 1 1 1 4 2 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 3 9 8 5 2 4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 37 2 84 68 65 77 93 52 35 51 22 32 0 1 0 13 29 16 0 0 0 0 0 0
SpindlePole 6 0 2 2 0 0 1 1 0 0 0 2 3 2 1 4 10 3 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 2 1 18 0 1 0 6 0 0 0 1 2 2 0 5 0 1 1 2
Unique Cell Count 233 160 307 251 292 435 537 545 395 624 472 668 116 218 252 329 688 342 254 185 317 239 315 415
Labelled Cell Count 472 303 675 591 584 911 989 891 747 1066 827 1123 181 312 345 439 951 507 254 185 317 239 315 415


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 7.4 7.5 8.5 8.8 8.5 8.3 9.0 9.1 8.9 9.4 9.1 10.0 9.6 9.7 6.7 8.5 10.6 9.5 9.6 10.0
Std Deviation (1e-4) 1.4 1.5 1.5 1.5 1.6 1.4 1.6 1.8 1.6 1.6 1.7 1.7 1.4 1.3 1.5 1.3 1.9 2.1 2.0 1.6 1.9
Intensity Change (Log2) – – – 0.17 0.23 0.18 0.14 0.26 0.27 0.23 0.32 0.28 0.4 0.34 0.36 -0.18 0.17 0.49 0.34 0.35 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.0 -1.1 -0.7 -5.3 -7.9 -4.6 -7.5 -6.0 -7.3 -0.5 -3.4 -4.7 -4.5 -4.3 -4.4
Bud 1.8 0.3 -0.9 -1.1 -2.3 -0.8 -2.0 -1.3 -1.0 -1.9 -1.3 -2.6 -3.4 -3.6 -3.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.5 -5.3 -5.0 -7.0 -10.3 -4.9 -8.2 -7.2 -8.6 3.8 4.6 7.5 1.3 1.3 4.6
Cytoplasm 0.8 -0.7 -1.5 -1.3 -2.7 -1.9 -4.0 -1.7 -3.5 0.8 2.1 1.5 4.0 2.9 2.3
Endoplasmic Reticulum 0.9 2.9 5.2 3.6 5.7 3.7 4.1 3.7 3.1 0 1.2 0.7 1.5 2.6 1.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 3.3 0 0 0 0 0 0 0 0 3.8 5.3
Mitochondria 2.5 1.8 4.1 4.8 3.6 6.2 8.6 8.4 8.3 -10.2 -13.5 -15.3 -14.3 -19.2 -15.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.1 -1.4 -3.1 -3.4 -6.8 -6.5 -7.8 -9.0 -10.1 -6.3 -8.2 -9.0 -8.2 -10.6 -8.0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.2 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.5482 12.2593 9.9041 9.8343 10.0671 10.137 6.9195 8.8163 8.7863 8.8378 7.7125 8.7521 6.4533 8.9565 8.6315 7.8293 8.5518 8.0358
Actin 0.0724 0.0185 0.045 0.0216 0.0149 0.0254 0.0562 0.1299 0.0189 0.0106 0.0461 0.0102 0.019 0.0014 0.0174 0.0929 0.0306 0.0289
Bud 0.001 0.0016 0.0014 0.0042 0.002 0.0011 0.0018 0.0005 0.0014 0.0006 0.001 0.0006 0.0009 0.0006 0.001 0.0009 0.0015 0.0014
Bud Neck 0.0094 0.0066 0.04 0.0329 0.0063 0.0491 0.0116 0.0059 0.0693 0.1129 0.0797 0.0456 0.0107 0.0114 0.1114 0.173 0.0461 0.0785
Bud Periphery 0.0057 0.0102 0.0101 0.0283 0.014 0.0148 0.0059 0.0013 0.0056 0.0084 0.0044 0.0084 0.0053 0.0016 0.0042 0.0063 0.0092 0.0156
Bud Site 0.0356 0.2404 0.0672 0.0151 0.0076 0.0034 0.052 0.2597 0.1614 0.009 0.0051 0.0044 0.058 0.5228 0.252 0.0174 0.0062 0.0094
Cell Periphery 0.5853 0.5452 0.6607 0.5997 0.6582 0.7188 0.5956 0.5379 0.6309 0.755 0.3497 0.7748 0.8136 0.428 0.4692 0.5523 0.5895 0.691
Cytoplasm 0.006 0.011 0.0068 0.012 0.005 0.0071 0.0061 0.0147 0.0093 0.0067 0.002 0.0048 0.0076 0.0157 0.0066 0.0033 0.0032 0.009
Cytoplasmic Foci 0.0061 0.0003 0.0053 0.0008 0.0102 0.0008 0.0392 0.0003 0.0004 0.0004 0.0142 0.0003 0.0001 0.0001 0.0007 0.0019 0.0573 0.0009
Eisosomes 0.2075 0.145 0.0798 0.1913 0.1078 0.1546 0.1587 0.043 0.0908 0.0767 0.1721 0.1313 0.0751 0.015 0.0982 0.1191 0.1272 0.1125
Endoplasmic Reticulum 0.0123 0.006 0.0119 0.0551 0.0429 0.0045 0.0013 0.0017 0.0013 0.0087 0.0031 0.0055 0.0012 0.0002 0.0015 0.0019 0.018 0.0099
Endosome 0.0061 0.0001 0.0066 0.0011 0.0115 0.0012 0.0167 0 0.0001 0.0001 0.0437 0.0002 0 0 0.0022 0.002 0.0084 0.0189
Golgi 0.0052 0.0004 0.0074 0.0036 0.0058 0.0018 0.0202 0.0004 0.0004 0.0009 0.0466 0.0008 0.0001 0 0.0023 0.0072 0.0166 0.0089
Lipid Particles 0.005 0.0004 0.0088 0.0023 0.0072 0.0008 0.0032 0.0002 0.0003 0.0006 0.0174 0.0004 0.0002 0.0001 0.0041 0.0048 0.0126 0.0009
Mitochondria 0.0175 0.0051 0.0136 0.0138 0.0436 0.0061 0.0056 0.0012 0.0033 0.0023 0.15 0.0066 0.0009 0.0002 0.0188 0.008 0.0446 0.0066
None 0.0065 0.0008 0.0015 0.0014 0.0048 0.0019 0.0016 0.0004 0.0006 0.0009 0.0008 0.0008 0.0006 0.0004 0.0005 0.0007 0.0011 0.0008
Nuclear Periphery 0.0043 0.0014 0.0058 0.0025 0.023 0.0014 0.0007 0.0005 0.0005 0.0009 0.0015 0.0006 0.0006 0.0002 0.0004 0.0005 0.0025 0.0016
Nucleolus 0.0005 0.0004 0.0024 0.0009 0.0029 0.0004 0.0003 0.0001 0.0005 0.0004 0.0004 0.0003 0.0003 0.0002 0.0007 0.0007 0.0005 0.0003
Nucleus 0.0008 0.0005 0.005 0.001 0.0095 0.0005 0.0003 0.0003 0.0005 0.0004 0.0005 0.0003 0.0003 0.0003 0.0007 0.0005 0.0006 0.0004
Peroxisomes 0.0049 0.0002 0.0034 0.0004 0.0054 0.0003 0.0197 0.0004 0.0005 0.0002 0.0571 0.0003 0.0002 0.0001 0.0008 0.0028 0.018 0.0004
Punctate Nuclear 0.0017 0 0.0078 0.0001 0.005 0.0003 0.0004 0 0 0 0.0006 0 0 0 0 0.0001 0.0003 0.0001
Vacuole 0.0053 0.0054 0.0083 0.0107 0.0092 0.0051 0.0026 0.0017 0.0038 0.0038 0.0023 0.0032 0.0052 0.0015 0.0063 0.0032 0.0047 0.0033
Vacuole Periphery 0.0009 0.0003 0.0014 0.0012 0.003 0.0006 0.0003 0 0.0001 0.0003 0.0018 0.0004 0.0001 0 0.0011 0.0004 0.0014 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.6681 99.2973 80.3813 82.6616 55.8951 76.8578 104.8752 97.1425 72.2274 73.9424
Translational Efficiency 0.6693 0.6076 0.7698 0.5026 0.6649 0.6678 0.5592 0.5145 0.5905 0.4451

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cortical ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; localizes to the mother cell in small-budded cells and to the bud in medium- and large-budded cells; mRNA is transported to the bud tip by an actomyosin-driven process
Localization
Cell Percentages cell periphery (91%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-2

Ist2

Ist2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ist2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available