Standard name | |
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Human Ortholog | |
Description | High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 13 | 4 | 4 | 20 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 5 | 1 | 3 | 11 | 11 | – | 18 | 17 | 10 | 7 | 8 | 11 | 33 | 46 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3 | 2 | 1 | 10 | 2 | 30 | – | 46 | 42 | 44 | 36 | 52 | 0 | 8 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 4 |
Nucleus | 307 | 154 | 94 | 251 | 392 | 308 | – | 543 | 389 | 464 | 284 | 430 | 302 | 336 | 317 | 25 | 14 | 15 | 311 | 156 | 96 | 48 | 186 | 177 |
Nuclear Periphery | 0 | 1 | 0 | 1 | 0 | 2 | – | 3 | 3 | 4 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 23 | 13 | 12 | 74 | 101 | 148 | – | 373 | 272 | 367 | 243 | 338 | 28 | 85 | 126 | 2 | 5 | 5 | 9 | 6 | 5 | 6 | 71 | 100 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Vac/Vac Membrane | 4 | 1 | 1 | 4 | 3 | 2 | – | 6 | 1 | 3 | 1 | 0 | 2 | 3 | 7 | 0 | 0 | 0 | 4 | 5 | 2 | 2 | 43 | 109 |
Unique Cell Count | 324 | 163 | 103 | 295 | 466 | 408 | 822 | 569 | 691 | 424 | 632 | 326 | 413 | 455 | 30 | 17 | 16 | 328 | 173 | 105 | 62 | 316 | 408 | |
Labelled Cell Count | 346 | 176 | 109 | 344 | 510 | 501 | 996 | 737 | 899 | 581 | 852 | 346 | 466 | 513 | 30 | 21 | 20 | 328 | 173 | 105 | 62 | 316 | 408 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 27.7 | 33.4 | 17.6 | 17.9 | 21.4 | 19.3 | – | 17.9 | 17.6 | 17.0 | 15.9 | 15.6 | 30.7 | 33.0 | 33.1 | 47.7 | 46.6 | 45.6 | 27.2 | 28.5 | 32.0 |
Std Deviation (1e-4) | 8.2 | 12.2 | 4.6 | 5.3 | 6.7 | 6.3 | – | 6.2 | 6.8 | 6.0 | 6.4 | 5.7 | 9.7 | 11.9 | 12.8 | 22.0 | 19.1 | 12.6 | 14.9 | 8.8 | 9.4 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.29 | 0.13 | – | 0.02 | -0.0 | -0.05 | -0.14 | -0.18 | 0.8 | 0.91 | 0.91 | 1.44 | 1.4 | 1.37 | 0.63 | 0.69 | 0.86 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.4 | – | 2.0 | 2.4 | 2.2 | 2.7 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.6 | -1.9 | -3.5 | – | -5.2 | -4.8 | -5.0 | -4.9 | -4.8 | 0.5 | -2.4 | -4.5 | -1.2 | -1.1 | 0.3 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.9 | 2.3 | 4.8 | – | 6.5 | 6.8 | 7.9 | 8.3 | 7.9 | -0.9 | 2.1 | 3.4 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 28.8128 | 26.5252 | 25.9252 | 22.9352 | 15.4734 | 26.3085 | 31.3874 | 28.175 | 25.0335 | 24.8594 | 22.5024 | 26.8272 | 34.2607 | 31.4801 | 29.2808 | 27.0711 | 28.2219 | 30.3251 |
Actin | 0.0152 | 0.0074 | 0.0036 | 0.0398 | 0.0689 | 0 | 0.0077 | 0.0009 | 0.0137 | 0.0253 | 0 | 0 | 0.0049 | 0 | 0.0017 | 0.0037 | 0 | 0 |
Bud | 0.0005 | 0 | 0.0002 | 0.0013 | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0001 | 0.0007 | 0.0018 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0011 | 0.0001 | 0.0004 | 0.0023 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0007 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.001 | 0.0009 | 0.0004 | 0.0074 | 0.0009 | 0 | 0.0003 | 0.0006 | 0.0014 | 0.0004 | 0 | 0 | 0.0086 | 0 | 0.0005 | 0.0002 | 0 | 0 |
Cell Periphery | 0.0004 | 0 | 0.0005 | 0.0009 | 0.0021 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0008 | 0.0001 | 0.0014 | 0.003 | 0.0063 | 0 | 0.0021 | 0.0005 | 0.0049 | 0.0002 | 0 | 0 | 0.0004 | 0 | 0 | 0.0006 | 0 | 0 |
Cytoplasmic Foci | 0.0064 | 0.0002 | 0.0067 | 0.0008 | 0.0145 | 0.0001 | 0.0074 | 0.0004 | 0.0054 | 0.0021 | 0 | 0 | 0.0122 | 0 | 0.0004 | 0.0115 | 0 | 0 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0013 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0008 | 0.0059 | 0.0078 | 0 | 0.0011 | 0.0005 | 0.003 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0012 | 0 | 0 |
Endosome | 0.0073 | 0.0002 | 0.0061 | 0.0148 | 0.0445 | 0 | 0.0035 | 0.0017 | 0.0057 | 0.0032 | 0 | 0 | 0.0021 | 0 | 0.0001 | 0.0062 | 0 | 0 |
Golgi | 0.0039 | 0.0003 | 0.0019 | 0.0014 | 0.0095 | 0 | 0.001 | 0.0003 | 0.0022 | 0.0047 | 0 | 0 | 0.0012 | 0 | 0.0002 | 0.0024 | 0 | 0 |
Lipid Particles | 0.0128 | 0.0003 | 0.0082 | 0.0008 | 0.0215 | 0 | 0.002 | 0.0002 | 0.0011 | 0.0057 | 0 | 0 | 0.0037 | 0 | 0.0014 | 0.0191 | 0 | 0 |
Mitochondria | 0.0072 | 0.0006 | 0.0101 | 0.0051 | 0.0125 | 0.0012 | 0.0129 | 0.0033 | 0.0055 | 0.0046 | 0.0006 | 0.0004 | 0.0041 | 0.0007 | 0.0077 | 0.0018 | 0.0016 | 0.0004 |
None | 0.0022 | 0.0001 | 0.0013 | 0.0016 | 0.0426 | 0 | 0.0012 | 0.0002 | 0.0013 | 0.0007 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0.0006 | 0 | 0 |
Nuclear Periphery | 0.0061 | 0.0004 | 0.0034 | 0.0294 | 0.0196 | 0.0002 | 0.0196 | 0.0042 | 0.0127 | 0.0068 | 0.0002 | 0.0001 | 0.0013 | 0.0003 | 0.0002 | 0.0011 | 0.0001 | 0.0001 |
Nucleolus | 0.0794 | 0.0394 | 0.0344 | 0.1723 | 0.1942 | 0.1591 | 0.0467 | 0.0274 | 0.0265 | 0.0571 | 0.2498 | 0.1285 | 0.057 | 0.0274 | 0.0207 | 0.0503 | 0.1872 | 0.0947 |
Nucleus | 0.8435 | 0.9486 | 0.9126 | 0.7054 | 0.4813 | 0.8376 | 0.8868 | 0.955 | 0.9081 | 0.8818 | 0.7487 | 0.8702 | 0.8844 | 0.9714 | 0.9644 | 0.8967 | 0.8102 | 0.9041 |
Peroxisomes | 0.0053 | 0.0003 | 0.0033 | 0.0002 | 0.0487 | 0 | 0.0024 | 0.0003 | 0.0004 | 0.0016 | 0 | 0 | 0.0045 | 0 | 0.0008 | 0.0024 | 0 | 0 |
Punctate Nuclear | 0.0016 | 0.0004 | 0.0017 | 0.0014 | 0.0136 | 0 | 0.0012 | 0.0022 | 0.004 | 0.0034 | 0.0001 | 0 | 0.0086 | 0 | 0.0001 | 0.001 | 0.0002 | 0 |
Vacuole | 0.0014 | 0.0001 | 0.0011 | 0.0026 | 0.0034 | 0.0006 | 0.0011 | 0.0008 | 0.0013 | 0.0002 | 0.0001 | 0.0002 | 0.003 | 0 | 0.0005 | 0.0005 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0018 | 0.0002 | 0.0018 | 0.0026 | 0.0042 | 0.0005 | 0.0019 | 0.0009 | 0.0016 | 0.001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0005 | 0.0003 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 121.3745 | 121.6531 | 134.1878 | 101.279 | 114.449 | 158.0671 | 147.9364 | 148.0878 | 145.8171 | 111.6543 |
Translational Efficiency | 2.4065 | 2.5044 | 1.89 | 3.5231 | 2.259 | 2.3889 | 2.0354 | 2.3702 | 2.3163 | 2.206 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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