Standard name | |
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Human Ortholog | |
Description | Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 5 | 2 | 1 | 0 | 1 | – | 1 | 9 | 2 | 1 | 1 | 2 |
Bud | 3 | 7 | 7 | 5 | 16 | 18 | 24 | 53 | – | 48 | 32 | 26 | 2 | 0 | 0 | 0 | 1 | – | 4 | 2 | 0 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | – | 1 | 2 | 0 | 0 | 0 | 2 |
Bud Site | 1 | 0 | 1 | 0 | 0 | 2 | 1 | 2 | – | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 85 | 84 | 60 | 31 | 44 | 75 | 103 | 140 | – | 137 | 142 | 156 | 66 | 32 | 64 | 29 | 18 | – | 15 | 18 | 8 | 13 | 7 | 7 |
Endoplasmic Reticulum | 5 | 7 | 0 | 1 | 2 | 6 | 1 | 11 | – | 0 | 2 | 7 | 18 | 33 | 8 | 3 | 0 | – | 0 | 1 | 1 | 0 | 3 | 5 |
Endosome | 433 | 221 | 252 | 107 | 215 | 47 | 50 | 69 | – | 13 | 10 | 13 | 68 | 19 | 50 | 25 | 10 | – | 540 | 343 | 323 | 240 | 224 | 237 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 6 | 3 | 5 | 3 | 3 | 3 |
Mitochondria | 26 | 64 | 42 | 57 | 106 | 336 | 247 | 408 | – | 498 | 344 | 466 | 0 | 0 | 0 | 6 | 3 | – | 20 | 21 | 29 | 21 | 28 | 25 |
Nucleus | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 1 | 2 | 0 | 2 | 1 | 2 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 2 | 1 | 1 | 4 | 2 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 3 | 2 | 3 | 2 | 0 | 4 |
Nucleolus | 6 | 4 | 2 | 5 | 3 | 5 | 2 | 13 | – | 2 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 1 | 1 | 0 | 0 |
Peroxisomes | 14 | 3 | 43 | 15 | 36 | 15 | 20 | 33 | – | 10 | 7 | 5 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 1 | 1 | 0 |
SpindlePole | 87 | 29 | 108 | 55 | 71 | 53 | 50 | 100 | – | 68 | 30 | 36 | 31 | 12 | 14 | 16 | 4 | – | 10 | 12 | 16 | 6 | 5 | 6 |
Vac/Vac Membrane | 191 | 122 | 36 | 9 | 28 | 14 | 18 | 12 | – | 17 | 16 | 21 | 45 | 24 | 42 | 12 | 11 | – | 65 | 66 | 26 | 54 | 54 | 74 |
Unique Cell Count | 622 | 413 | 382 | 219 | 365 | 456 | 404 | 639 | 617 | 459 | 587 | 197 | 114 | 159 | 71 | 41 | 681 | 497 | 427 | 352 | 335 | 377 | ||
Labelled Cell Count | 858 | 542 | 551 | 287 | 522 | 574 | 523 | 844 | 798 | 590 | 736 | 237 | 125 | 182 | 95 | 49 | 681 | 497 | 427 | 352 | 335 | 377 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.4 | 4.5 | 4.2 | 4.2 | 3.4 | 3.3 | 3.6 | – | 3.1 | 3.3 | 3.2 | 6.0 | 6.2 | 6.2 | 5.3 | 6.1 | – | 6.0 | 6.6 | 6.8 |
Std Deviation (1e-4) | 0.7 | 1.0 | 1.2 | 0.9 | 1.4 | 1.1 | 1.1 | 0.9 | – | 0.6 | 0.9 | 0.6 | 0.8 | 0.8 | 0.8 | 1.2 | 2.0 | – | 1.2 | 1.3 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.1 | -0.38 | -0.45 | -0.3 | – | -0.52 | -0.43 | -0.48 | 0.42 | 0.48 | 0.47 | 0.25 | 0.44 | – | 0.43 | 0.56 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | -0.5 | -1.4 | 0.3 | 3.4 | 2.4 | – | 2.5 | 5.1 | 4.0 | 4.9 | 3.0 | 6.2 | 4.9 | 4.4 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 6.0 | 10.9 | 0 | 0 | 0 | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -7.2 | -9.3 | -7.4 | -4.9 | -5.2 | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -4.8 | -3.7 | -4.4 | -0.6 | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -4.9 | -3.7 | -4.4 | -3.0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | -3.3 | -3.9 | -4.9 | -1.0 | -2.6 | – |
Vacuole | -2.4 | -0.9 | -3.9 | -2.8 | -5.5 | – | -4.6 | -3.6 | -3.8 | 4.4 | 3.3 | 5.1 | 1.9 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.017 | 0.0002 | 0.0007 | 0.0022 | 0.0062 | 0.0001 | 0.0097 | 0.0009 | 0.0285 | 0.0001 | 0 | 0.0009 | 0 | 0.0006 | 0.0281 | 0 | 0 | 0.0002 |
Bud | 0.0004 | 0.0004 | 0.0005 | 0.0007 | 0.0002 | 0.0001 | 0.0008 | 0.0011 | 0.0082 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Bud Neck | 0.0041 | 0.0007 | 0.0038 | 0.0003 | 0.0006 | 0.0004 | 0.0063 | 0.0008 | 0.0011 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0005 | 0.0003 | 0.0034 | 0.0002 | 0.0007 |
Bud Periphery | 0.0007 | 0.0003 | 0.0006 | 0.0008 | 0.0003 | 0.0001 | 0.0017 | 0.0021 | 0.0076 | 0.0004 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 |
Bud Site | 0.003 | 0.0049 | 0.0036 | 0.0018 | 0.0009 | 0.0002 | 0.0386 | 0.0029 | 0.0039 | 0.0003 | 0.0002 | 0.0003 | 0 | 0.0021 | 0.0006 | 0.001 | 0.0001 | 0.0003 |
Cell Periphery | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0018 | 0.0004 | 0.0005 | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0001 |
Cytoplasm | 0.0255 | 0.107 | 0.0756 | 0.0907 | 0.0239 | 0.0387 | 0.0272 | 0.0559 | 0.0272 | 0.0457 | 0.0168 | 0.0222 | 0.0005 | 0.1029 | 0.03 | 0.0077 | 0.0035 | 0.0243 |
Cytoplasmic Foci | 0.1377 | 0.1541 | 0.1636 | 0.1358 | 0.1417 | 0.1726 | 0.1721 | 0.1384 | 0.2002 | 0.1568 | 0.3178 | 0.1961 | 0.3261 | 0.1618 | 0.2493 | 0.1118 | 0.2483 | 0.2194 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.001 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0039 | 0.004 | 0.003 | 0.0042 | 0.001 | 0.0013 | 0.0045 | 0.0029 | 0.002 | 0.0055 | 0.0003 | 0.0014 | 0.0001 | 0.0111 | 0.0016 | 0.0016 | 0.0004 | 0.0051 |
Endosome | 0.5617 | 0.5014 | 0.4873 | 0.5787 | 0.6689 | 0.5776 | 0.5408 | 0.4881 | 0.4806 | 0.6222 | 0.6212 | 0.5623 | 0.1048 | 0.4093 | 0.4459 | 0.7371 | 0.596 | 0.5417 |
Golgi | 0.0173 | 0.0117 | 0.021 | 0.0234 | 0.0239 | 0.0237 | 0.02 | 0.0157 | 0.0304 | 0.0218 | 0.0211 | 0.0298 | 0.013 | 0.0242 | 0.0209 | 0.0171 | 0.0324 | 0.0322 |
Lipid Particles | 0.0815 | 0.0242 | 0.0469 | 0.0112 | 0.025 | 0.0746 | 0.0729 | 0.0458 | 0.0736 | 0.0149 | 0.0071 | 0.0735 | 0.4258 | 0.0711 | 0.0589 | 0.0615 | 0.0879 | 0.1085 |
Mitochondria | 0.0058 | 0.0018 | 0.011 | 0.0164 | 0.0328 | 0.0027 | 0.0196 | 0.0206 | 0.0161 | 0.0079 | 0.0007 | 0.007 | 0.0004 | 0.0062 | 0.0067 | 0.0009 | 0.002 | 0.0017 |
None | 0.0369 | 0.0957 | 0.1024 | 0.0271 | 0.0042 | 0.0526 | 0.0124 | 0.1507 | 0.0816 | 0.0677 | 0.0009 | 0.0578 | 0.0003 | 0.1394 | 0.0833 | 0.0081 | 0.0019 | 0.0033 |
Nuclear Periphery | 0.0137 | 0.0038 | 0.003 | 0.0055 | 0.0005 | 0.0012 | 0.0065 | 0.0012 | 0.0022 | 0.0028 | 0.0001 | 0.0011 | 0.0001 | 0.0049 | 0.0015 | 0.0012 | 0.0005 | 0.0021 |
Nucleolus | 0.0013 | 0.0014 | 0.0016 | 0.0004 | 0.0007 | 0.0007 | 0.0023 | 0.0009 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0037 | 0.0004 | 0.0103 | 0.0001 | 0.0007 |
Nucleus | 0.0024 | 0.0028 | 0.0014 | 0.0013 | 0.0005 | 0.001 | 0.0051 | 0.0011 | 0.0018 | 0.0009 | 0.0001 | 0.0005 | 0 | 0.0056 | 0.0004 | 0.0031 | 0.0001 | 0.0006 |
Peroxisomes | 0.0116 | 0.0035 | 0.0185 | 0.02 | 0.0297 | 0.0222 | 0.0141 | 0.009 | 0.0159 | 0.0062 | 0.0062 | 0.0204 | 0.1266 | 0.0078 | 0.0416 | 0.0029 | 0.0173 | 0.0195 |
Punctate Nuclear | 0.022 | 0.0075 | 0.0027 | 0.0013 | 0.0006 | 0.0022 | 0.0169 | 0.0013 | 0.0015 | 0.0005 | 0.0005 | 0.0015 | 0.0016 | 0.0041 | 0.0027 | 0.0072 | 0.0007 | 0.0051 |
Vacuole | 0.0448 | 0.0662 | 0.0445 | 0.0639 | 0.0305 | 0.0224 | 0.0213 | 0.0526 | 0.0121 | 0.0286 | 0.0053 | 0.0201 | 0.0003 | 0.0389 | 0.023 | 0.0192 | 0.006 | 0.0299 |
Vacuole Periphery | 0.0082 | 0.008 | 0.0079 | 0.0143 | 0.0077 | 0.0053 | 0.0043 | 0.0076 | 0.0036 | 0.0167 | 0.0013 | 0.0039 | 0.0002 | 0.0048 | 0.0043 | 0.0054 | 0.0025 | 0.0045 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.6877 | 4.0675 | 6.2042 | 10.5604 | 5.5717 | 5.0529 | 8.9679 | 10.255 | 8.8948 | 10.9127 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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