Standard name | |
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Human Ortholog | |
Description | Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 0 | 0 | 1 | 4 | 6 | 5 | 5 | 3 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 5 |
Bud | 0 | 0 | 0 | 1 | 6 | 0 | 1 | 1 | 5 | 6 | 1 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 6 | 2 | 1 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 9 | 24 | 34 | 11 | 7 | 25 | 21 | 20 | 13 | 40 | 30 | 32 | 12 | 6 | 9 | 9 | 22 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 272 | 594 | 563 | 424 | 376 | 438 | 509 | 408 | 424 | 447 | 269 | 355 | 232 | 405 | 466 | 358 | 246 | 294 | 257 | 583 | 557 | 216 | 312 | 363 |
Endoplasmic Reticulum | 2 | 3 | 7 | 7 | 6 | 21 | 56 | 15 | 23 | 40 | 21 | 24 | 1 | 1 | 3 | 19 | 10 | 7 | 1 | 2 | 12 | 2 | 6 | 2 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 2 | 2 | 0 | 1 | 1 |
Golgi | 0 | 0 | 3 | 0 | 1 | 1 | 3 | 5 | 0 | 3 | 1 | 2 | 0 | 1 | 0 | 2 | 3 | 2 | 0 | 1 | 2 | 3 | 4 | 4 |
Mitochondria | 0 | 4 | 0 | 0 | 6 | 3 | 6 | 3 | 19 | 13 | 16 | 35 | 4 | 3 | 4 | 8 | 2 | 1 | 0 | 3 | 2 | 6 | 9 | 9 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 2 | 4 | 8 | 4 | 7 | 3 | 6 | 0 | 2 | 4 | 0 | 2 | 0 | 0 | 3 | 2 | 2 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 |
Nucleolus | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 |
Vac/Vac Membrane | 1 | 8 | 12 | 11 | 29 | 30 | 55 | 135 | 46 | 101 | 61 | 71 | 3 | 0 | 5 | 11 | 10 | 28 | 2 | 4 | 3 | 1 | 2 | 3 |
Unique Cell Count | 274 | 608 | 587 | 440 | 418 | 497 | 629 | 580 | 515 | 625 | 371 | 506 | 237 | 411 | 478 | 375 | 266 | 313 | 266 | 609 | 600 | 247 | 351 | 408 |
Labelled Cell Count | 284 | 633 | 623 | 455 | 435 | 521 | 659 | 601 | 540 | 662 | 409 | 551 | 253 | 418 | 493 | 409 | 297 | 338 | 266 | 609 | 600 | 247 | 351 | 408 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 24.6 | 20.3 | 20.3 | 18.8 | 16.2 | 18.5 | 18.0 | 18.1 | 16.3 | 17.6 | 16.3 | 15.9 | 22.4 | 22.0 | 20.1 | 19.5 | 24.4 | 23.3 | 20.6 | 23.1 | 24.9 |
Std Deviation (1e-4) | 4.3 | 5.6 | 6.5 | 4.6 | 7.1 | 4.6 | 4.7 | 4.8 | 5.1 | 5.7 | 5.4 | 4.5 | 8.7 | 9.5 | 4.2 | 10.8 | 14.2 | 11.7 | 7.3 | 9.7 | 8.4 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.33 | -0.14 | -0.17 | -0.17 | -0.32 | -0.21 | -0.32 | -0.36 | 0.14 | 0.11 | -0.01 | -0.06 | 0.27 | 0.2 | 0.02 | 0.19 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.6 | -3.3 | -0.6 | -2.1 | -1.9 | -2.7 | 0.4 | 1.4 | 0.4 | -0.4 | -3.4 | -3.2 | -2.5 | 1.4 | -2.7 |
Cytoplasm | 0.4 | -3.8 | -4.8 | -8.1 | -11.7 | -7.4 | -11.4 | -10.4 | -11.6 | 1.4 | 2.4 | 1.4 | -0.3 | -2.1 | -1.3 |
Endoplasmic Reticulum | 0.5 | 0.3 | 3.1 | 6.1 | 1.8 | 3.3 | 4.7 | 4.0 | 3.5 | 0 | 0 | 0 | 3.6 | 2.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.7 | 3.5 | 5.1 | 6.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.5 | 3.9 | 3.4 | 5.1 | 10.9 | 5.1 | 8.4 | 8.2 | 7.5 | 0 | 0 | -1.3 | 0.9 | 1.5 | 4.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.6864 | 22.3801 | 20.0568 | 18.0059 | 18.6742 | 20.7452 | 16.3353 | 24.494 | 20.98 | 22.3408 | 24.5846 | 24.0096 | 15.4802 | 18.2545 | 17.5082 | 16.5251 | 16.9292 | 16.9564 |
Actin | 0.0213 | 0.0004 | 0.0093 | 0.0302 | 0.0357 | 0.004 | 0.0649 | 0.0028 | 0.0091 | 0.0001 | 0 | 0.0031 | 0.023 | 0.0043 | 0.0182 | 0.0009 | 0.0014 | 0.0013 |
Bud | 0.0011 | 0.0002 | 0.0007 | 0.0012 | 0.0012 | 0.0003 | 0.0031 | 0.0003 | 0.001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0009 | 0.0003 | 0.0006 | 0.0014 | 0.0005 | 0.0017 | 0.0062 | 0.0007 | 0.0004 | 0.0005 | 0.0002 | 0.0005 | 0.0013 | 0.001 | 0.0006 | 0.0086 | 0.0004 | 0.0006 |
Bud Periphery | 0.0013 | 0.0001 | 0.0007 | 0.0014 | 0.0008 | 0.0002 | 0.0052 | 0.0002 | 0.0023 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 |
Bud Site | 0.0052 | 0.0004 | 0.0012 | 0.0031 | 0.0009 | 0.0001 | 0.006 | 0.0466 | 0.0065 | 0 | 0 | 0 | 0.0054 | 0.0117 | 0.0051 | 0.0069 | 0.0001 | 0 |
Cell Periphery | 0.0008 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0009 | 0.0004 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0006 | 0 | 0 |
Cytoplasm | 0.8496 | 0.9871 | 0.9101 | 0.7805 | 0.8661 | 0.959 | 0.6477 | 0.9327 | 0.8975 | 0.9953 | 0.9958 | 0.9775 | 0.9097 | 0.9772 | 0.9272 | 0.9263 | 0.9818 | 0.9788 |
Cytoplasmic Foci | 0.0216 | 0.0005 | 0.012 | 0.0149 | 0.0139 | 0.0054 | 0.0468 | 0.0011 | 0.0108 | 0.0012 | 0.0004 | 0.001 | 0.0128 | 0.0012 | 0.0082 | 0.0063 | 0.007 | 0.0017 |
Eisosomes | 0.0008 | 0 | 0.0002 | 0.0003 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0218 | 0.0008 | 0.0045 | 0.0088 | 0.0017 | 0.0031 | 0.0165 | 0.012 | 0.0182 | 0.0006 | 0.0006 | 0.0056 | 0.0117 | 0.0008 | 0.0017 | 0.001 | 0.0016 | 0.0034 |
Endosome | 0.021 | 0.0003 | 0.0224 | 0.0362 | 0.0318 | 0.0033 | 0.0882 | 0.0008 | 0.0195 | 0.0005 | 0.0002 | 0.0062 | 0.007 | 0.0005 | 0.0165 | 0.0047 | 0.0039 | 0.0015 |
Golgi | 0.0094 | 0.0001 | 0.012 | 0.0269 | 0.0065 | 0.0033 | 0.0161 | 0.0003 | 0.0045 | 0 | 0 | 0.0016 | 0.0035 | 0.0002 | 0.0057 | 0.001 | 0.0004 | 0.0002 |
Lipid Particles | 0.0054 | 0 | 0.0017 | 0.0148 | 0.0178 | 0.0012 | 0.0075 | 0 | 0.0015 | 0 | 0 | 0.0002 | 0.0016 | 0.0001 | 0.0053 | 0.0043 | 0 | 0.0001 |
Mitochondria | 0.0085 | 0.0066 | 0.012 | 0.056 | 0.0016 | 0.013 | 0.0225 | 0.0001 | 0.0183 | 0 | 0 | 0.0005 | 0.0011 | 0 | 0.0023 | 0.0024 | 0.0002 | 0.0002 |
None | 0.0096 | 0.0002 | 0.0005 | 0.0011 | 0.0003 | 0.0003 | 0.013 | 0.0002 | 0.0015 | 0.0003 | 0.0002 | 0.0003 | 0.0018 | 0.0007 | 0.0009 | 0.0095 | 0.0009 | 0.0004 |
Nuclear Periphery | 0.0065 | 0.0003 | 0.0028 | 0.002 | 0.001 | 0.0007 | 0.0132 | 0.0007 | 0.0029 | 0.0002 | 0.0005 | 0.0021 | 0.0055 | 0.0003 | 0.0009 | 0.0005 | 0.0004 | 0.0096 |
Nucleolus | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0013 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0103 | 0 | 0 |
Nucleus | 0.0037 | 0.0021 | 0.0016 | 0.0021 | 0.0017 | 0.0016 | 0.0123 | 0.0009 | 0.0015 | 0.0008 | 0.0019 | 0.001 | 0.0042 | 0.0007 | 0.0011 | 0.0071 | 0.0012 | 0.0012 |
Peroxisomes | 0.0066 | 0 | 0.0023 | 0.0097 | 0.0127 | 0.0017 | 0.0033 | 0.0001 | 0.0014 | 0 | 0 | 0 | 0.0081 | 0 | 0.0038 | 0.002 | 0.0002 | 0 |
Punctate Nuclear | 0.0008 | 0.0001 | 0.0003 | 0.0005 | 0.0003 | 0.0001 | 0.0081 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0009 | 0.0001 | 0.0008 | 0.0038 | 0.0001 | 0.0002 |
Vacuole | 0.0032 | 0.0002 | 0.0034 | 0.0058 | 0.0046 | 0.0006 | 0.0128 | 0.0001 | 0.0014 | 0.0002 | 0.0001 | 0.0001 | 0.0012 | 0.0002 | 0.0007 | 0.0028 | 0.0003 | 0.0003 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0014 | 0.002 | 0.0007 | 0.0002 | 0.0041 | 0 | 0.0008 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0003 | 0.0003 | 0.0001 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.5569 | 48.5844 | 41.9206 | 58.082 | 46.7038 | 65.6876 | 78.2551 | 70.5511 | 71.6417 | 58.0577 |
Translational Efficiency | 2.4857 | 3.1421 | 2.0399 | 2.1377 | 1.9752 | 2.0926 | 2.2439 | 1.9354 | 1.8028 | 1.7067 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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