Standard name
Human Ortholog
Description Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0 0 0.05 0 0 0 0.06 0.08 0.06 0.05 0 0 0 0.08 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.96 0.96 0.9 0.88 0.81 0.7 0.82 0.72 0.73 0.7 0.98 0.99 0.97 0.95 0.92 0.94 0.97 0.96 0.93 0.88 0.89 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.09 0 0 0.06 0.06 0.05 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.06 0.09 0.23 0.09 0.16 0.16 0.14 0 0 0 0 0 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 1 4 6 5 5 3 19 0 0 0 0 0 0 0 0 2 1 1 5
Bud 0 0 0 1 6 0 1 1 5 6 1 6 1 0 0 0 0 0 1 3 6 2 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 9 24 34 11 7 25 21 20 13 40 30 32 12 6 9 9 22 6 0 0 0 0 0 0
Cytoplasm 272 594 563 424 376 438 509 408 424 447 269 355 232 405 466 358 246 294 257 583 557 216 312 363
Endoplasmic Reticulum 2 3 7 7 6 21 56 15 23 40 21 24 1 1 3 19 10 7 1 2 12 2 6 2
Endosome 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 2 2 0 0 2 2 0 1 1
Golgi 0 0 3 0 1 1 3 5 0 3 1 2 0 1 0 2 3 2 0 1 2 3 4 4
Mitochondria 0 4 0 0 6 3 6 3 19 13 16 35 4 3 4 8 2 1 0 3 2 6 9 9
Nucleus 0 0 0 0 2 2 4 8 4 7 3 6 0 2 4 0 2 0 0 3 2 2 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2
Nucleolus 0 0 1 1 1 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 4
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1
Vac/Vac Membrane 1 8 12 11 29 30 55 135 46 101 61 71 3 0 5 11 10 28 2 4 3 1 2 3
Unique Cell Count 274 608 587 440 418 497 629 580 515 625 371 506 237 411 478 375 266 313 266 609 600 247 351 408
Labelled Cell Count 284 633 623 455 435 521 659 601 540 662 409 551 253 418 493 409 297 338 266 609 600 247 351 408


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.6 20.3 20.3 18.8 16.2 18.5 18.0 18.1 16.3 17.6 16.3 15.9 22.4 22.0 20.1 19.5 24.4 23.3 20.6 23.1 24.9
Std Deviation (1e-4) 4.3 5.6 6.5 4.6 7.1 4.6 4.7 4.8 5.1 5.7 5.4 4.5 8.7 9.5 4.2 10.8 14.2 11.7 7.3 9.7 8.4
Intensity Change (Log2) -0.11 -0.33 -0.14 -0.17 -0.17 -0.32 -0.21 -0.32 -0.36 0.14 0.11 -0.01 -0.06 0.27 0.2 0.02 0.19 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 4.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.6 -3.3 -0.6 -2.1 -1.9 -2.7 0.4 1.4 0.4 -0.4 -3.4 -3.2 -2.5 1.4 -2.7
Cytoplasm 0.4 -3.8 -4.8 -8.1 -11.7 -7.4 -11.4 -10.4 -11.6 1.4 2.4 1.4 -0.3 -2.1 -1.3
Endoplasmic Reticulum 0.5 0.3 3.1 6.1 1.8 3.3 4.7 4.0 3.5 0 0 0 3.6 2.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.7 3.5 5.1 6.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.5 3.9 3.4 5.1 10.9 5.1 8.4 8.2 7.5 0 0 -1.3 0.9 1.5 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.6864 22.3801 20.0568 18.0059 18.6742 20.7452 16.3353 24.494 20.98 22.3408 24.5846 24.0096 15.4802 18.2545 17.5082 16.5251 16.9292 16.9564
Actin 0.0213 0.0004 0.0093 0.0302 0.0357 0.004 0.0649 0.0028 0.0091 0.0001 0 0.0031 0.023 0.0043 0.0182 0.0009 0.0014 0.0013
Bud 0.0011 0.0002 0.0007 0.0012 0.0012 0.0003 0.0031 0.0003 0.001 0 0 0 0.0003 0.0001 0.0003 0.0002 0.0001 0.0001
Bud Neck 0.0009 0.0003 0.0006 0.0014 0.0005 0.0017 0.0062 0.0007 0.0004 0.0005 0.0002 0.0005 0.0013 0.001 0.0006 0.0086 0.0004 0.0006
Bud Periphery 0.0013 0.0001 0.0007 0.0014 0.0008 0.0002 0.0052 0.0002 0.0023 0 0 0 0.0002 0.0001 0.0002 0.0003 0 0.0001
Bud Site 0.0052 0.0004 0.0012 0.0031 0.0009 0.0001 0.006 0.0466 0.0065 0 0 0 0.0054 0.0117 0.0051 0.0069 0.0001 0
Cell Periphery 0.0008 0.0001 0.0003 0.0005 0.0002 0.0001 0.0009 0.0004 0.0003 0 0 0 0.0001 0.0006 0.0001 0.0006 0 0
Cytoplasm 0.8496 0.9871 0.9101 0.7805 0.8661 0.959 0.6477 0.9327 0.8975 0.9953 0.9958 0.9775 0.9097 0.9772 0.9272 0.9263 0.9818 0.9788
Cytoplasmic Foci 0.0216 0.0005 0.012 0.0149 0.0139 0.0054 0.0468 0.0011 0.0108 0.0012 0.0004 0.001 0.0128 0.0012 0.0082 0.0063 0.007 0.0017
Eisosomes 0.0008 0 0.0002 0.0003 0 0 0.0004 0 0.0001 0 0 0 0.0001 0.0001 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.0218 0.0008 0.0045 0.0088 0.0017 0.0031 0.0165 0.012 0.0182 0.0006 0.0006 0.0056 0.0117 0.0008 0.0017 0.001 0.0016 0.0034
Endosome 0.021 0.0003 0.0224 0.0362 0.0318 0.0033 0.0882 0.0008 0.0195 0.0005 0.0002 0.0062 0.007 0.0005 0.0165 0.0047 0.0039 0.0015
Golgi 0.0094 0.0001 0.012 0.0269 0.0065 0.0033 0.0161 0.0003 0.0045 0 0 0.0016 0.0035 0.0002 0.0057 0.001 0.0004 0.0002
Lipid Particles 0.0054 0 0.0017 0.0148 0.0178 0.0012 0.0075 0 0.0015 0 0 0.0002 0.0016 0.0001 0.0053 0.0043 0 0.0001
Mitochondria 0.0085 0.0066 0.012 0.056 0.0016 0.013 0.0225 0.0001 0.0183 0 0 0.0005 0.0011 0 0.0023 0.0024 0.0002 0.0002
None 0.0096 0.0002 0.0005 0.0011 0.0003 0.0003 0.013 0.0002 0.0015 0.0003 0.0002 0.0003 0.0018 0.0007 0.0009 0.0095 0.0009 0.0004
Nuclear Periphery 0.0065 0.0003 0.0028 0.002 0.001 0.0007 0.0132 0.0007 0.0029 0.0002 0.0005 0.0021 0.0055 0.0003 0.0009 0.0005 0.0004 0.0096
Nucleolus 0.0002 0 0.0001 0.0004 0.0001 0 0.0013 0 0.0001 0 0 0 0.0001 0 0 0.0103 0 0
Nucleus 0.0037 0.0021 0.0016 0.0021 0.0017 0.0016 0.0123 0.0009 0.0015 0.0008 0.0019 0.001 0.0042 0.0007 0.0011 0.0071 0.0012 0.0012
Peroxisomes 0.0066 0 0.0023 0.0097 0.0127 0.0017 0.0033 0.0001 0.0014 0 0 0 0.0081 0 0.0038 0.002 0.0002 0
Punctate Nuclear 0.0008 0.0001 0.0003 0.0005 0.0003 0.0001 0.0081 0.0001 0.0006 0 0 0.0001 0.0009 0.0001 0.0008 0.0038 0.0001 0.0002
Vacuole 0.0032 0.0002 0.0034 0.0058 0.0046 0.0006 0.0128 0.0001 0.0014 0.0002 0.0001 0.0001 0.0012 0.0002 0.0007 0.0028 0.0003 0.0003
Vacuole Periphery 0.0011 0.0001 0.0014 0.002 0.0007 0.0002 0.0041 0 0.0008 0.0001 0 0.0001 0.0004 0 0.0003 0.0003 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.5569 48.5844 41.9206 58.082 46.7038 65.6876 78.2551 70.5511 71.6417 58.0577
Translational Efficiency 2.4857 3.1421 2.0399 2.1377 1.9752 2.0926 2.2439 1.9354 1.8028 1.7067

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for destruction; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Fes1

Fes1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fes1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available