Standard name
Human Ortholog
Description Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.4 0.34 0.45 0.39 0.27 0.15 0.21 0.19 0.2 0.16 0.12 0.18 0.3 0.36 0.3 0.29 0.29 0.3 0.33 0.3 0.25 0.12 0.13 0.16
Bud 0.21 0.35 0.25 0.29 0.28 0.23 0.23 0.23 0.19 0.17 0.16 0.2 0.18 0.19 0.13 0.15 0.16 0.12 0.36 0.35 0.27 0.48 0.47 0.51
Bud Neck 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0.16 0.22 0.23 0.24 0.17 0.1
Bud Site 0.24 0.17 0.26 0.15 0.15 0.16 0.1 0.08 0.09 0.07 0.07 0.07 0.18 0.05 0 0.08 0 0 – – – – – –
Cell Periphery 0 0.06 0.07 0.13 0 0 0 0 0.06 0.05 0.06 0.09 0.12 0.22 0.2 0.15 0.11 0.12 0 0 0 0 0 0
Cytoplasm 0.19 0.19 0.23 0.26 0.33 0.29 0.36 0.36 0.28 0.28 0.33 0.29 0.27 0.3 0.37 0.24 0.28 0.35 0 0 0.05 0 0.07 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.06 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.16 0 0.06 0 0.17 0.18 0.14 0.41 0.42 0.46 0.32 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.19 0.11 0 0.05 0 0.13 0 0 0 0.06 0.05 0.05 0.22 0.27 0.29 0.15 0.09 0.08 0 0 0 0 0 0
SpindlePole 0.06 0.13 0.22 0.23 0.26 0.17 0.15 0.18 0.09 0.09 0.08 0.11 0.12 0.08 0 0.23 0.21 0.12 0 0 0.06 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 83 60 118 107 103 46 100 83 63 50 37 60 77 114 112 59 44 34 78 63 82 25 45 57
Bud 45 62 65 80 105 68 109 99 61 54 48 67 45 62 49 30 24 14 86 73 90 105 161 180
Bud Neck 3 3 8 12 12 12 24 14 10 5 7 9 0 1 1 2 9 0 38 45 76 52 58 35
Bud Site 50 30 69 42 56 48 50 35 29 22 22 24 46 17 15 16 4 3 – – – – – –
Cell Periphery 9 11 18 36 3 13 20 18 20 16 18 30 30 72 76 30 17 14 4 2 5 0 2 1
Cytoplasm 39 33 62 70 123 89 176 158 87 86 98 97 69 96 140 47 43 40 3 7 15 8 24 32
Endoplasmic Reticulum 4 5 3 2 5 6 10 10 1 6 4 8 4 7 11 8 9 4 1 1 1 1 8 3
Endosome 6 7 3 2 5 8 5 5 5 4 2 9 14 15 8 12 6 3 4 3 9 4 6 6
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 1 2 0 0 2 3 4
Mitochondria 50 28 2 16 8 50 86 60 128 129 137 104 12 7 12 6 5 5 3 4 14 3 8 10
Nucleus 10 4 8 4 14 3 15 21 5 5 8 8 5 2 1 2 1 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 1 0 0 2
Nucleolus 1 1 2 1 5 9 7 12 6 3 6 5 4 1 0 4 0 1 0 0 0 0 0 0
Peroxisomes 40 20 11 13 7 38 20 12 11 17 16 15 57 86 109 30 14 9 4 1 6 3 5 3
SpindlePole 12 24 57 63 99 51 71 78 27 29 25 36 31 26 15 45 32 14 7 3 20 4 8 5
Vac/Vac Membrane 1 5 9 1 10 6 11 13 7 2 7 19 0 2 0 10 7 3 0 1 5 5 5 4
Unique Cell Count 210 178 265 272 378 302 484 436 315 309 299 330 255 321 377 200 153 115 239 210 331 219 346 353
Labelled Cell Count 354 294 435 449 555 447 704 618 460 428 436 491 394 509 550 304 220 146 239 210 331 219 346 353


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 5.9 6.4 5.9 6.3 5.2 5.3 5.6 4.7 4.5 4.5 5.2 6.3 6.4 6.5 9.2 9.5 9.2 5.8 6.1 6.4
Std Deviation (1e-4) 1.6 1.1 1.1 1.1 1.0 1.1 1.1 1.2 1.1 0.9 1.0 1.3 1.2 1.1 1.1 1.8 2.2 1.8 1.0 1.7 1.8
Intensity Change (Log2) – – – -0.14 -0.03 -0.31 -0.27 -0.2 -0.45 -0.51 -0.53 -0.31 -0.03 -0.02 0.02 0.51 0.56 0.52 -0.14 -0.09 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.2 -4.5 -7.7 -6.9 -7.2 0 0 0 -7.0 -3.4 -2.2 -3.9 -3.3 -3.2 -2.7
Bud 1.3 0.9 -0.6 -0.6 -0.6 0 0 0 -1.2 -1.9 -1.5 -3.8 -2.5 -2.1 -2.7
Bud Neck 0.9 0.1 0.6 1.3 0.1 0 0 0 -0.2 0 0 0 0 1.4 0
Bud Site -3.0 -3.5 -3.0 -5.6 -6.5 0 0 0 -6.3 -2.2 -7.1 -8.2 -5.0 -6.1 -5.4
Cell Periphery 2.5 -4.2 -1.3 -1.6 -1.5 0 0 0 1.0 2.0 5.2 4.7 2.9 1.5 1.7
Cytoplasm 0.6 2.5 1.6 3.6 3.6 1.2 1.2 2.5 1.6 1.0 1.8 3.7 0 1.1 2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 1.5 0 0 0
Endosome 0 0 1.3 0 0 0 0 0 1.4 2.8 2.5 0 2.9 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 0 6.5 6.9 5.9 0 0 0 9.7 2.8 0 2.1 0 0 0
Nucleus -1.2 0.5 -1.7 0.1 1.2 -1.2 -1.1 -0.2 -0.4 -0.8 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.9 0 1.8 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.4 -1.7 3.6 0 -1.0 0 0 0 0.2 6.2 7.3 7.9 4.1 2.1 1.5
SpindlePole 0.5 1.4 -1.4 -2.4 -1.2 0 0 0 -3.5 -2.8 -4.6 -6.9 0.3 -0.1 -2.1
Vacuole 0 -0.6 -1.0 -0.9 -0.3 -0.9 -2.4 -0.8 1.4 0 0 0 0.9 0.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6591 1.3635 1.1386 1.1893 0.7933 1.3672 1.6714 1.9858 1.6179 1.7675 1.1833 2.0799 1.7109 1.7152 1.4753 1.9072 1.5171 1.9567
Actin 0.1394 0.1089 0.1249 0.0652 0.5245 0.1932 0.2022 0.0633 0.1162 0.3059 0.3507 0.2034 0.1813 0.105 0.0849 0.1572 0.3873 0.1206
Bud 0.0221 0.06 0.226 0.0942 0.1164 0.0136 0.0205 0.0535 0.2979 0.1286 0.0512 0.011 0.0656 0.0763 0.3187 0.1123 0.0605 0.0155
Bud Neck 0.1344 0.0201 0.017 0.0946 0.0037 0.6027 0.0931 0.0154 0.006 0.0011 0.02 0.6148 0.0551 0.0118 0.0037 0.0028 0.0606 0.6601
Bud Periphery 0.0538 0.0349 0.316 0.4935 0.1494 0.0338 0.0439 0.0486 0.3499 0.3562 0.1761 0.0236 0.0912 0.0523 0.3618 0.5752 0.2362 0.0523
Bud Site 0.2536 0.5333 0.0715 0.0115 0.0145 0.0172 0.247 0.6347 0.0973 0.0131 0.0568 0.0272 0.2027 0.5982 0.069 0.076 0.0567 0.0235
Cell Periphery 0.0193 0.0057 0.0077 0.0029 0.0019 0.0042 0.0309 0.0131 0.0041 0.0026 0.0074 0.004 0.0079 0.0074 0.003 0.0064 0.0162 0.0072
Cytoplasm 0.0915 0.1127 0.076 0.0464 0.0297 0.0157 0.0965 0.0994 0.0531 0.0725 0.0578 0.0266 0.0946 0.0785 0.0794 0.0079 0.1559 0.0349
Cytoplasmic Foci 0.0907 0.0755 0.0827 0.0279 0.019 0.0338 0.1137 0.0313 0.0062 0.0107 0.0843 0.0286 0.0822 0.0242 0.0251 0.0094 0.0096 0.0433
Eisosomes 0.0012 0.0004 0.0007 0.0004 0.0011 0.0004 0.0013 0.0014 0.0005 0.0003 0.0006 0.0007 0.0012 0.0005 0.0002 0.0029 0.002 0.0004
Endoplasmic Reticulum 0.0103 0.0022 0.0042 0.0014 0.0009 0.0011 0.0052 0.0022 0.0013 0.002 0.0069 0.001 0.024 0.0007 0.0011 0.0003 0.0006 0.0004
Endosome 0.0394 0.0043 0.005 0.0054 0.013 0.0265 0.0284 0.0017 0.0175 0.0303 0.0875 0.0104 0.0428 0.0019 0.014 0.0002 0.001 0.0006
Golgi 0.0201 0.0061 0.003 0.0028 0.0116 0.0195 0.0192 0.0043 0.0058 0.0113 0.03 0.0082 0.0322 0.006 0.0083 0.0004 0.0027 0.002
Lipid Particles 0.0195 0.0021 0.0044 0.0011 0.0616 0.0045 0.0126 0.001 0.0023 0.0011 0.0113 0.0038 0.0106 0.0012 0.0047 0.0002 0.0007 0.0006
Mitochondria 0.017 0.0007 0.0015 0.0835 0.0028 0.0047 0.0094 0.002 0.0195 0.0356 0.0063 0.0058 0.0216 0.0134 0.0079 0.0012 0.001 0.0005
None 0.0154 0.0181 0.0165 0.0292 0.0096 0.0011 0.0198 0.0165 0.012 0.007 0.0042 0.0105 0.02 0.0116 0.0067 0.0433 0.003 0.0316
Nuclear Periphery 0.0142 0.0004 0.0008 0.0009 0.0021 0.001 0.0171 0.0003 0.0012 0.001 0.0047 0.0003 0.0243 0.0002 0.0015 0.0001 0.0002 0
Nucleolus 0.0049 0.0003 0.0037 0.0003 0.0003 0.0004 0.001 0.0005 0.0012 0.0001 0.0006 0.0005 0.0006 0.0004 0.0002 0.0006 0.0002 0.0002
Nucleus 0.0104 0.0007 0.0011 0.0013 0.001 0.0007 0.0069 0.0006 0.0013 0.0008 0.0017 0.0006 0.0082 0.0006 0.0013 0.0005 0.0003 0.0002
Peroxisomes 0.0197 0.0099 0.0174 0.0301 0.0263 0.0217 0.014 0.007 0.0022 0.0013 0.0294 0.0154 0.0121 0.0058 0.0056 0.0009 0.0042 0.0051
Punctate Nuclear 0.0138 0.0015 0.0175 0.0006 0.0061 0.0014 0.009 0.001 0.0008 0.0003 0.0023 0.001 0.0063 0.0022 0.0014 0.0009 0.0007 0.0006
Vacuole 0.0078 0.002 0.0022 0.0055 0.0039 0.0024 0.0039 0.0021 0.0028 0.0134 0.008 0.0021 0.0066 0.0015 0.0011 0.0011 0.0005 0.0003
Vacuole Periphery 0.0016 0.0002 0.0003 0.0014 0.0006 0.0005 0.0043 0.0001 0.0008 0.0048 0.002 0.0005 0.0091 0.0002 0.0004 0.0002 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.4389 10.4655 11.0017 15.4262 12.3329 9.7506 16.7543 19.4915 16.8538 19.1652
Translational Efficiency 1.3191 1.2648 1.4385 0.8751 1.1968 1.7114 1.1011 0.8799 1.0657 0.8688

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
Localization
Cell Percentages actin (32%), bud neck (22%), mixed (24%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Exo84

Exo84


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Exo84-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available