Standard name
Human Ortholog
Description Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0.09 0.05 0.05 0.05 0.05 0.06 – 0.05 0.07 0.06 0 0 0 0.07 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.08 0.08 0.12 – 0.12 0.15 0.09 0 0 0 0.12 0.14 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0.15 0.07 0.19 0.09 0.09 0.05 0.07 0 0.08 0.06
Golgi 0 0.1 0 0 0 0 0 0 – 0 0 0 0.17 0.28 0.31 0.37 0.21 0.23 0.07 0.08 0 0.05 0.12 0.11
Mitochondria 0.89 0.82 0.96 0.94 0.93 0.89 0.91 0.81 – 0.84 0.85 0.92 0.86 0.61 0.52 0.09 0.07 0.11 0.69 0.74 0.78 0.73 0.58 0.6
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0.06 0.11 0.07 0.12 0.06 0 0 0 0.06 0.07 0.07
SpindlePole 0 0 0 0 0 0.07 0.08 0.12 – 0.13 0.07 0.07 0 0 0 0.08 0.08 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.19 0 0.06 0.09 0.05 0 0 – 0 0 0 0.14 0.25 0.12 0.09 0.14 0.25 0 0 0 0.05 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 1 1 0 0 – 0 0 0 1 2 1 3 1 5 1 1 1 2 5 12
Bud 3 1 12 14 23 24 31 40 – 34 24 36 1 2 4 7 4 2 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 1 3 – 2 1 2 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 1 0 0 2 16 16 9 – 14 7 16 0 0 0 2 2 1 – – – – – –
Cell Periphery 0 4 1 11 2 5 0 1 – 3 0 4 1 6 6 3 6 10 0 0 0 0 0 0
Cytoplasm 2 4 5 11 23 43 52 84 – 76 51 52 0 4 7 13 23 22 0 0 0 0 3 0
Endoplasmic Reticulum 0 1 1 1 4 4 1 1 – 1 0 1 2 1 3 2 5 4 0 0 1 1 2 1
Endosome 9 2 1 5 9 2 0 7 – 5 0 0 0 11 30 8 31 11 12 5 9 5 16 14
Golgi 1 11 0 2 1 0 0 0 – 1 0 0 11 48 64 40 34 29 10 9 1 6 25 28
Mitochondria 111 90 133 292 420 468 567 558 – 542 289 515 55 105 106 10 11 14 99 89 108 103 126 155
Nucleus 0 0 1 0 6 21 14 24 – 11 8 6 1 0 2 5 4 1 0 0 0 0 1 0
Nuclear Periphery 2 4 0 2 13 3 2 2 – 1 2 2 0 1 1 5 6 2 2 0 5 1 0 2
Nucleolus 9 4 7 6 10 11 2 7 – 4 2 6 2 3 4 3 7 3 2 1 0 1 1 1
Peroxisomes 1 1 1 12 6 9 8 11 – 19 0 2 0 11 23 7 19 7 4 1 5 8 16 18
SpindlePole 2 0 2 4 12 35 47 82 – 82 23 37 1 1 9 9 12 10 3 0 0 0 2 0
Vac/Vac Membrane 14 21 3 18 39 24 24 12 – 23 7 3 9 42 24 10 23 31 2 4 1 6 8 15
Unique Cell Count 125 110 139 309 453 528 626 693 645 340 557 64 171 205 107 159 126 143 120 138 141 219 259
Labelled Cell Count 154 145 167 378 571 666 765 841 818 414 682 84 237 284 127 188 152 143 120 138 141 219 259


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 7.7 4.6 5.3 4.7 3.9 3.1 3.4 – 3.0 2.9 2.9 7.9 8.2 7.8 7.7 7.8 8.1 8.3 8.7 8.5
Std Deviation (1e-4) 1.3 1.7 1.1 1.4 1.2 1.5 1.3 1.5 – 1.3 1.3 1.0 1.1 1.5 1.5 1.5 1.5 1.5 1.3 3.4 1.4
Intensity Change (Log2) – – – 0.2 0.03 -0.22 -0.55 -0.45 – -0.59 -0.64 -0.67 0.78 0.83 0.77 0.74 0.76 0.82 0.85 0.93 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud -1.7 0 0 0 0 – 0 0 0 0 -3.1 -2.9 -0.6 -2.3 -2.6
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2.9
Cytoplasm 0 0.7 1.8 1.9 3.0 – 2.9 3.5 2.2 0 0 0 2.6 3.2 3.7
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 0 2.6 4.4 0 5.2 3.1
Golgi 0 0 0 0 0 – 0 0 0 0 6.8 7.3 7.9 5.8 6.0
Mitochondria -0.5 0 0 0 0 – 0 0 0 -2.5 -7.1 -8.7 -13.6 -15.3 -13.8
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 -0.3 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 2.6 3.8 0 3.9 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 2.5 2.5
Vacuole 1.7 2.6 1.3 0 0 – 0 0 0 0 5.6 3.2 2.5 3.8 5.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.4118 9.6874 8.6209 7.9333 7.8033 9.3509 8.8543 9.7925 9.0932 8.8315 7.7684 9.1059 9.6551 9.8454 9.4239 9.8735 8.492 9.0853
Actin 0.0204 0.0082 0.0231 0.0025 0.0017 0.0016 0.0023 0.0024 0.0045 0.0019 0.0007 0.0007 0.0076 0.0029 0.0016 0.001 0.0057 0.0028
Bud 0.0004 0.0068 0.003 0.0008 0.0233 0.0018 0.0001 0.0008 0.0006 0.0001 0.0001 0.0001 0.0001 0.0002 0.0006 0.0001 0.0034 0.0001
Bud Neck 0.0007 0.0021 0.0005 0.0138 0.0003 0.001 0.0003 0.0004 0.0002 0.001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0001
Bud Periphery 0.0008 0.0078 0.0055 0.0014 0.0457 0.0007 0.0002 0.0013 0.0022 0.0002 0.0002 0.0007 0.0002 0.0004 0.0006 0.0002 0.0101 0.0002
Bud Site 0.0024 0.0234 0.0048 0.0037 0.0047 0.0003 0.0042 0.0027 0.0019 0.0002 0.0003 0.0001 0.0007 0.0012 0.0012 0.0003 0.0004 0.0001
Cell Periphery 0.0006 0.0023 0.0012 0.0006 0.001 0.0003 0.0002 0.0005 0.0005 0.0002 0.0001 0.0003 0.0002 0.0004 0.0003 0.0003 0.0001 0.0001
Cytoplasm 0.0026 0.0004 0.0011 0.0065 0.0031 0.0021 0.0009 0.0002 0.001 0.0001 0 0 0.0001 0.0001 0.0008 0 0.0003 0
Cytoplasmic Foci 0.0197 0.0037 0.0125 0.0215 0.0078 0.0024 0.0078 0.0121 0.0052 0.0044 0.0051 0.0013 0.0053 0.0074 0.0025 0.0035 0.0012 0.001
Eisosomes 0.0003 0.0003 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0005 0.0001
Endoplasmic Reticulum 0.0033 0.0002 0.0007 0.001 0.0016 0.0001 0.0007 0.0001 0.0007 0 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0009 0
Endosome 0.0479 0.0249 0.0319 0.0184 0.0322 0.01 0.0101 0.0257 0.0107 0.0101 0.0069 0.0043 0.0106 0.0168 0.0107 0.0054 0.0073 0.004
Golgi 0.0273 0.0231 0.0291 0.0085 0.0195 0.0105 0.0128 0.0566 0.0152 0.0476 0.0099 0.0178 0.0151 0.0488 0.0132 0.0118 0.0126 0.0149
Lipid Particles 0.0453 0.0082 0.0234 0.0089 0.0148 0.0093 0.0362 0.0152 0.0154 0.0072 0.0087 0.0085 0.034 0.0274 0.009 0.0142 0.0127 0.0127
Mitochondria 0.7604 0.795 0.7842 0.8888 0.7997 0.9361 0.8792 0.8176 0.902 0.9152 0.9287 0.9496 0.8845 0.8461 0.926 0.9221 0.9195 0.9542
None 0.0049 0.0001 0.0026 0.0002 0.0006 0.0002 0.0059 0.0001 0.0006 0 0.0001 0 0.0003 0 0 0 0.0038 0
Nuclear Periphery 0.0109 0.0003 0.0018 0.0004 0.0004 0.0001 0.0044 0.0001 0.0032 0 0.0007 0.0001 0.0004 0.0001 0.0002 0.0004 0.0084 0.0001
Nucleolus 0.002 0.0091 0.0039 0.0003 0.0033 0.0012 0.0019 0.003 0.0005 0.0001 0.0002 0.0002 0.0009 0.0071 0.0003 0.0012 0.0003 0.0001
Nucleus 0.0043 0.0024 0.0023 0.0003 0.001 0.0043 0.0014 0.0004 0.0006 0.0001 0.0001 0.0001 0.0002 0.0006 0.0002 0.0002 0.0005 0
Peroxisomes 0.0179 0.0101 0.0305 0.0155 0.0145 0.0036 0.0162 0.0442 0.0168 0.009 0.0297 0.0049 0.018 0.0174 0.0118 0.0119 0.0084 0.0041
Punctate Nuclear 0.0029 0.0001 0.0016 0.0007 0.0001 0.0001 0.003 0.0001 0.0017 0 0.0001 0 0.0006 0.0001 0 0.0009 0.0013 0
Vacuole 0.0125 0.0391 0.0191 0.0025 0.0137 0.0072 0.0031 0.0105 0.0062 0.0009 0.0015 0.0048 0.007 0.0116 0.0083 0.015 0.0006 0.0011
Vacuole Periphery 0.0127 0.0324 0.0168 0.0035 0.011 0.007 0.0089 0.0062 0.0102 0.0014 0.0066 0.0062 0.0138 0.0109 0.0123 0.011 0.0019 0.0045

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.2229 28.8938 29.5731 21.2782 32.5139 28.9623 33.496 19.3779 20.0527 34.842
Translational Efficiency 2.297 1.8011 1.1526 1.3606 1.8048 2.3781 1.388 1.7219 1.3003 1.3106

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Ymc2

Ymc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available