Standard name | |
---|---|
Human Ortholog | |
Description | Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 |
Bud | 2 | 0 | 0 | 1 | 0 | 8 | 2 | – | 3 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 8 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 1 | 2 | 2 | 1 | 8 | – | 1 | 1 | 7 | 8 | 0 | 0 | 3 | 4 | 3 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 187 | 208 | 278 | 269 | 292 | 148 | 272 | – | 220 | 173 | 224 | 265 | 346 | 295 | 393 | 140 | 134 | 198 | 245 | 197 | 267 | 178 | 327 | 247 |
Endoplasmic Reticulum | 0 | 2 | 3 | 1 | 0 | 1 | 1 | – | 0 | 2 | 5 | 10 | 1 | 1 | 2 | 1 | 1 | 1 | 2 | 0 | 1 | 3 | 1 | 1 |
Endosome | 3 | 0 | 1 | 0 | 1 | 0 | 2 | – | 5 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 9 | 1 | 6 | 0 | 1 | 1 |
Golgi | 0 | 0 | 1 | 2 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 2 | 1 | 0 | 3 |
Mitochondria | 11 | 5 | 1 | 7 | 5 | 44 | 14 | – | 92 | 77 | 89 | 108 | 0 | 0 | 0 | 2 | 2 | 2 | 5 | 4 | 5 | 1 | 8 | 4 |
Nucleus | 7 | 0 | 2 | 2 | 0 | 1 | 14 | – | 12 | 12 | 26 | 20 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 9 | 0 | 0 | 0 | 0 | 1 | 0 | – | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 6 | 0 | 3 | 2 |
Vac/Vac Membrane | 10 | 2 | 2 | 11 | 40 | 19 | 82 | – | 77 | 63 | 88 | 109 | 4 | 1 | 3 | 30 | 34 | 20 | 8 | 2 | 2 | 1 | 2 | 2 |
Unique Cell Count | 223 | 215 | 285 | 283 | 317 | 200 | 364 | 352 | 291 | 379 | 459 | 350 | 296 | 396 | 158 | 153 | 212 | 296 | 218 | 299 | 197 | 361 | 279 | |
Labelled Cell Count | 233 | 220 | 289 | 295 | 340 | 224 | 396 | 414 | 333 | 446 | 527 | 351 | 297 | 403 | 179 | 177 | 230 | 296 | 218 | 299 | 197 | 361 | 279 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.4 | 6.2 | 6.2 | 7.4 | 5.4 | 7.1 | – | 6.4 | 6.2 | 7.7 | 6.9 | 7.1 | 7.0 | 7.3 | 9.9 | 10.4 | 10.2 | 6.3 | 6.0 | 5.9 |
Std Deviation (1e-4) | 0.9 | 0.9 | 0.9 | 1.3 | 1.5 | 1.4 | 1.6 | – | 1.4 | 1.7 | 21.2 | 1.4 | 1.4 | 1.3 | 1.3 | 1.8 | 2.6 | 1.8 | 2.9 | 2.5 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.01 | 0.27 | -0.19 | 0.2 | – | 0.05 | 0.01 | 0.33 | 0.17 | 0.21 | 0.18 | 0.25 | 0.68 | 0.76 | 0.73 | 0.03 | -0.05 | -0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.6 | -3.0 | -7.8 | -8.0 | – | -10.6 | -11.1 | -11.4 | -11.9 | 1.3 | 2.2 | 1.8 | -3.9 | -4.2 | -2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 8.1 | 2.9 | – | 9.2 | 9.2 | 8.6 | 8.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.6 | – | 2.3 | 2.7 | 3.9 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.5 | 5.7 | 4.7 | 8.2 | – | 8.1 | 7.9 | 8.4 | 8.6 | 0 | 0 | 0 | 7.1 | 7.8 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1295 | 5.2129 | 3.814 | 4.1552 | 3.3286 | 3.7813 | 4.4285 | 6.1799 | 5.3517 | 4.6232 | 3.9362 | 5.6091 | 8.3118 | 9.0535 | 8.3323 | 8.6085 | 7.8372 | 8.3008 |
Actin | 0.023 | 0.0003 | 0.0095 | 0.0001 | 0.0199 | 0.0026 | 0.0431 | 0.0009 | 0.0105 | 0.0007 | 0.0057 | 0.0013 | 0.0112 | 0.0003 | 0.018 | 0.0018 | 0.0005 | 0.0004 |
Bud | 0.0011 | 0.0001 | 0.0005 | 0.0001 | 0.002 | 0.0001 | 0.0008 | 0.0047 | 0.0009 | 0.0001 | 0.0008 | 0.0023 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0035 | 0.0002 | 0.001 | 0.0005 | 0.0007 | 0.0016 | 0.0009 | 0.0002 | 0.0015 | 0.0003 | 0.0007 | 0.0007 | 0.0041 | 0.0002 | 0.0005 | 0.0003 | 0.0004 | 0.0008 |
Bud Periphery | 0.0014 | 0 | 0.0004 | 0 | 0.0029 | 0.0001 | 0.0009 | 0.0028 | 0.0017 | 0 | 0.0013 | 0.0008 | 0.0004 | 0 | 0.0004 | 0 | 0 | 0 |
Bud Site | 0.0027 | 0.0003 | 0.001 | 0 | 0.004 | 0.0001 | 0.0034 | 0.0108 | 0.0094 | 0.0001 | 0.0007 | 0.0003 | 0.0042 | 0.0005 | 0.0021 | 0.0002 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0006 | 0.0012 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.8256 | 0.9712 | 0.9089 | 0.9711 | 0.8652 | 0.9348 | 0.5407 | 0.9359 | 0.8853 | 0.9866 | 0.779 | 0.9145 | 0.9159 | 0.9871 | 0.9251 | 0.9815 | 0.9676 | 0.9614 |
Cytoplasmic Foci | 0.0249 | 0.0026 | 0.0103 | 0.0025 | 0.015 | 0.0072 | 0.0652 | 0.0038 | 0.0131 | 0.0017 | 0.0218 | 0.0101 | 0.0146 | 0.0017 | 0.0026 | 0.0032 | 0.0046 | 0.0038 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0048 | 0.002 | 0.0025 | 0.0019 | 0.0041 | 0.0033 | 0.0087 | 0.0007 | 0.0026 | 0.0016 | 0.0041 | 0.0015 | 0.0038 | 0.0005 | 0.004 | 0.0014 | 0.0022 | 0.0014 |
Endosome | 0.0316 | 0.0023 | 0.009 | 0.0054 | 0.0214 | 0.0112 | 0.0857 | 0.0003 | 0.0188 | 0.0007 | 0.0181 | 0.0106 | 0.005 | 0.0003 | 0.0029 | 0.001 | 0.0013 | 0.0036 |
Golgi | 0.008 | 0.0001 | 0.0028 | 0.0001 | 0.0073 | 0.0018 | 0.0286 | 0.0001 | 0.0185 | 0 | 0.0032 | 0.0041 | 0.0021 | 0 | 0.0014 | 0.0001 | 0.0001 | 0.0017 |
Lipid Particles | 0.0107 | 0 | 0.0029 | 0 | 0.0047 | 0.0032 | 0.0332 | 0.0001 | 0.0036 | 0 | 0.0032 | 0.0053 | 0.0023 | 0 | 0.0015 | 0 | 0 | 0.0003 |
Mitochondria | 0.0112 | 0.0003 | 0.0019 | 0.0002 | 0.0058 | 0.0011 | 0.0163 | 0.0177 | 0.0083 | 0.0001 | 0.0527 | 0.0021 | 0.0006 | 0.0001 | 0.0011 | 0.0001 | 0.0001 | 0.0002 |
None | 0.0127 | 0.0033 | 0.0155 | 0.0036 | 0.0101 | 0.0079 | 0.0378 | 0.0164 | 0.0027 | 0.0026 | 0.0375 | 0.0203 | 0.0168 | 0.0031 | 0.0133 | 0.004 | 0.0158 | 0.0101 |
Nuclear Periphery | 0.0047 | 0.004 | 0.0032 | 0.0029 | 0.006 | 0.0032 | 0.0374 | 0.0005 | 0.0017 | 0.0009 | 0.023 | 0.0117 | 0.0029 | 0.0004 | 0.003 | 0.0008 | 0.0006 | 0.0009 |
Nucleolus | 0.0006 | 0 | 0.0007 | 0 | 0.0003 | 0.0001 | 0.0039 | 0.0002 | 0.0011 | 0 | 0.0015 | 0.0008 | 0.0001 | 0 | 0.0006 | 0 | 0 | 0 |
Nucleus | 0.0129 | 0.0114 | 0.0115 | 0.0095 | 0.0155 | 0.0144 | 0.0337 | 0.0028 | 0.0039 | 0.0038 | 0.0118 | 0.0059 | 0.008 | 0.0051 | 0.0174 | 0.0045 | 0.0054 | 0.0142 |
Peroxisomes | 0.0109 | 0 | 0.0121 | 0 | 0.0052 | 0.0025 | 0.0109 | 0.0001 | 0.0035 | 0 | 0.0099 | 0.0012 | 0.0037 | 0 | 0.001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0028 | 0.0011 | 0.0043 | 0.0003 | 0.0074 | 0.0027 | 0.0401 | 0.001 | 0.0004 | 0.0004 | 0.016 | 0.002 | 0.003 | 0.0003 | 0.0035 | 0.0005 | 0.0005 | 0.0004 |
Vacuole | 0.0043 | 0.0006 | 0.0015 | 0.0016 | 0.0019 | 0.0016 | 0.0046 | 0.0005 | 0.0092 | 0.0003 | 0.0043 | 0.0017 | 0.0005 | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0.0003 |
Vacuole Periphery | 0.002 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0004 | 0.0036 | 0.0001 | 0.002 | 0.0001 | 0.0045 | 0.0029 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 56.28 | 62.3571 | 67.3042 | 86.8822 | 83.2468 | 66.5479 | 77.3786 | 85.5915 | 89.861 | 79.0595 |
Translational Efficiency | 2.9522 | 2.3873 | 2.0401 | 2.1702 | 1.9052 | 2.7333 | 1.7351 | 2.2361 | 2.1824 | 2.0082 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|