Standard name | |
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Human Ortholog | |
Description | Translational elongation factor EF-1 alpha; GTP-bound active form, binds to and delivers aminoacylated tRNA to the A-site of ribosomes for elongation of nascent polypeptides; moonlighting function as an actin binding and bundling protein; association with GTPase Rho1p on the vacuolar membrane may facilitate F-actin remodeling; involved in tRNA re-export from the nucleus; Tef2p-RFP levels increase during replicative aging |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 92 | 11 | 27 | 33 | 12 | 9 | 12 | 28 | 12 | 22 | 14 | 18 | 53 | 88 | 116 | 86 | 95 | 72 | 32 | 18 | 1 | 17 | 26 | 62 |
Cytoplasm | 97 | 9 | 145 | 184 | 302 | 359 | 325 | 349 | 268 | 264 | 225 | 267 | 115 | 158 | 166 | 84 | 87 | 66 | 180 | 63 | 148 | 159 | 182 | 197 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 10 | 5 | 9 | 6 | 0 | 0 | 0 | 2 | 2 | 1 | 6 | 1 | 2 | 4 | 4 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Unique Cell Count | 153 | 17 | 158 | 196 | 305 | 365 | 339 | 376 | 286 | 285 | 240 | 285 | 153 | 228 | 257 | 144 | 159 | 122 | 230 | 86 | 158 | 189 | 226 | 277 |
Labelled Cell Count | 189 | 20 | 172 | 220 | 314 | 369 | 340 | 385 | 292 | 292 | 249 | 291 | 169 | 247 | 285 | 172 | 184 | 139 | 230 | 86 | 158 | 189 | 226 | 277 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 650.2 | 642.0 | 476.4 | 481.6 | 395.4 | 297.8 | 284.2 | 284.5 | 261.6 | 251.1 | 238.8 | 238.6 | 552.9 | 556.9 | 582.4 | 678.7 | 670.0 | 679.0 | 622.3 | 599.2 | 615.2 |
Std Deviation (1e-4) | 102.9 | 157.9 | 97.9 | 84.9 | 63.9 | 67.9 | 63.7 | 73.8 | 60.2 | 59.0 | 58.8 | 57.5 | 111.6 | 119.6 | 117.7 | 97.4 | 120.2 | 114.4 | 113.0 | 156.2 | 163.0 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.27 | -0.68 | -0.75 | -0.74 | -0.86 | -0.92 | -1.0 | -1.0 | 0.21 | 0.23 | 0.29 | 0.51 | 0.49 | 0.51 | 0.39 | 0.33 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.1 | -4.8 | -6.1 | -5.2 | -3.3 | -4.6 | -3.0 | -3.6 | -3.6 | 3.5 | 4.5 | 5.8 | 7.6 | 7.8 | 7.3 |
Cytoplasm | 0.8 | 4.0 | 3.7 | 1.9 | 0.4 | 0.8 | 0.3 | 0.8 | 0.8 | -4.0 | -5.3 | -6.2 | -6.8 | -7.4 | -7.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0021 | 0.0007 | 0.0009 | 0.0046 | 0.0054 | 0.0062 | 0.0027 | 0.0011 | 0.0851 | 0.0001 | 0.0001 | 0.0168 | – | – | – | – | – | – |
Bud | 0.0035 | 0.0033 | 0.0039 | 0.0024 | 0.0028 | 0.0062 | 0.0072 | 0.0044 | 0.0247 | 0.0001 | 0.0065 | 0.0046 | – | – | – | – | – | – |
Bud Neck | 0.0018 | 0.0011 | 0.0019 | 0.002 | 0.0021 | 0.0046 | 0.0008 | 0.0005 | 0.0016 | 0.0002 | 0.0002 | 0.0036 | – | – | – | – | – | – |
Bud Periphery | 0.0051 | 0.0018 | 0.0034 | 0.0017 | 0.0034 | 0.007 | 0.0109 | 0.0009 | 0.0711 | 0 | 0.0018 | 0.0051 | – | – | – | – | – | – |
Bud Site | 0.0017 | 0.002 | 0.0027 | 0.0005 | 0.0072 | 0.0012 | 0.008 | 0.001 | 0.0063 | 0 | 0.0011 | 0.0019 | – | – | – | – | – | – |
Cell Periphery | 0.1276 | 0.0358 | 0.1031 | 0.014 | 0.0188 | 0.0569 | 0.0253 | 0.0033 | 0.0203 | 0 | 0.0066 | 0.0136 | – | – | – | – | – | – |
Cytoplasm | 0.3408 | 0.7569 | 0.5086 | 0.2465 | 0.4253 | 0.3694 | 0.0138 | 0.7472 | 0.1442 | 0.984 | 0.0153 | 0.4502 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0036 | 0.0003 | 0.0017 | 0.0014 | 0.0201 | 0.0026 | 0.0498 | 0.0004 | 0.1536 | 0.0001 | 0.0104 | 0.0026 | – | – | – | – | – | – |
Eisosomes | 0.0063 | 0.0003 | 0.0009 | 0.0028 | 0.0001 | 0.0019 | 0.0027 | 0 | 0.0044 | 0 | 0.0005 | 0.0036 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0202 | 0.0023 | 0.0022 | 0.002 | 0.0249 | 0.0108 | 0.0093 | 0.0003 | 0.0062 | 0 | 0.0001 | 0.0076 | – | – | – | – | – | – |
Endosome | 0.001 | 0.0003 | 0.0008 | 0.0008 | 0.0473 | 0.0025 | 0.053 | 0.0004 | 0.0475 | 0 | 0.0004 | 0.0149 | – | – | – | – | – | – |
Golgi | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0242 | 0.0006 | 0.0245 | 0 | 0.0268 | 0 | 0 | 0.0022 | – | – | – | – | – | – |
Lipid Particles | 0.0034 | 0.0005 | 0.0025 | 0.0005 | 0.0028 | 0.0037 | 0.0225 | 0 | 0.0168 | 0 | 0.0006 | 0.0032 | – | – | – | – | – | – |
Mitochondria | 0.0408 | 0.0071 | 0.0224 | 0.2496 | 0.0154 | 0.0775 | 0.2608 | 0.0527 | 0.0972 | 0.0004 | 0.0766 | 0.0936 | – | – | – | – | – | – |
None | 0.0097 | 0.0021 | 0.0065 | 0.038 | 0.0046 | 0.0064 | 0.0326 | 0.0019 | 0.039 | 0.0001 | 0.0384 | 0.0238 | – | – | – | – | – | – |
Nuclear Periphery | 0.0917 | 0.0252 | 0.0275 | 0.0892 | 0.074 | 0.1019 | 0.0151 | 0.0076 | 0.0085 | 0.0013 | 0.0015 | 0.0464 | – | – | – | – | – | – |
Nucleolus | 0.0726 | 0.0079 | 0.0605 | 0.0438 | 0.0095 | 0.0401 | 0.2834 | 0.0035 | 0.1077 | 0 | 0.553 | 0.0454 | – | – | – | – | – | – |
Nucleus | 0.0978 | 0.0879 | 0.0872 | 0.2399 | 0.1471 | 0.1433 | 0.0917 | 0.1362 | 0.0383 | 0.013 | 0.2365 | 0.0789 | – | – | – | – | – | – |
Peroxisomes | 0.0009 | 0.0002 | 0.0007 | 0.0031 | 0.0013 | 0.0011 | 0.0056 | 0.0006 | 0.0118 | 0 | 0.0012 | 0.0018 | – | – | – | – | – | – |
Punctate Nuclear | 0.0006 | 0.0002 | 0.0003 | 0.0008 | 0.0007 | 0.0007 | 0.0062 | 0.0001 | 0.0033 | 0 | 0.0012 | 0.0031 | – | – | – | – | – | – |
Vacuole | 0.1362 | 0.0544 | 0.1319 | 0.0219 | 0.0904 | 0.0931 | 0.035 | 0.0221 | 0.0564 | 0.0003 | 0.026 | 0.069 | – | – | – | – | – | – |
Vacuole Periphery | 0.0323 | 0.0095 | 0.0301 | 0.0342 | 0.0727 | 0.0624 | 0.0389 | 0.0161 | 0.0291 | 0.0003 | 0.0218 | 0.108 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16958.533 | 17918.809 | 18171.3034 | 15684.7149 | 17595.3859 | 17032.8353 | 16829.5697 | 15729.5032 | 16756.5084 | 14640.5285 |
Translational Efficiency | 0.6565 | 0.645 | 0.6438 | 0.6135 | 0.6701 | 0.6771 | 0.7227 | 0.821 | 0.7059 | 0.842 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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