Standard name | |
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Human Ortholog | |
Description | Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 5 | 9 | 6 | 11 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 9 | 7 | 3 | 8 | 11 | 7 | 13 | 15 | 14 | 13 | 18 | 3 | 2 | 6 | 8 | 14 | 12 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 232 | 254 | 260 | 126 | 197 | 246 | 220 | 234 | 202 | 164 | 125 | 169 | 197 | 284 | 291 | 230 | 260 | 269 | 230 | 247 | 241 | 153 | 217 | 144 |
Endoplasmic Reticulum | 2 | 4 | 4 | 0 | 10 | 7 | 36 | 53 | 31 | 26 | 20 | 31 | 2 | 3 | 5 | 8 | 12 | 7 | 1 | 2 | 13 | 3 | 7 | 4 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 6 | 4 | 2 | 7 | 0 | 0 | 0 | 3 | 5 | 0 | 0 | 0 | 3 | 0 | 1 | 1 |
Mitochondria | 1 | 0 | 0 | 1 | 1 | 3 | 1 | 4 | 8 | 3 | 13 | 18 | 0 | 1 | 0 | 4 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 1 | 0 | 4 | 0 | 2 | 10 | 3 | 9 | 4 | 6 | 1 | 0 | 0 | 9 | 15 | 3 | 2 | 1 | 1 | 1 | 1 | 0 |
Unique Cell Count | 234 | 259 | 265 | 128 | 214 | 259 | 268 | 305 | 255 | 216 | 174 | 238 | 199 | 287 | 295 | 246 | 281 | 279 | 242 | 258 | 273 | 166 | 238 | 156 |
Labelled Cell Count | 237 | 267 | 273 | 130 | 221 | 270 | 276 | 324 | 276 | 227 | 191 | 261 | 203 | 290 | 304 | 263 | 310 | 293 | 242 | 258 | 273 | 166 | 238 | 156 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.9 | 36.4 | 30.9 | 31.0 | 36.7 | 37.4 | 39.5 | 38.7 | 41.0 | 42.8 | 40.4 | 44.8 | 41.8 | 59.6 | 64.8 | 35.6 | 41.7 | 45.1 | 22.4 | 24.2 | 28.3 |
Std Deviation (1e-4) | 4.5 | 8.4 | 11.0 | 11.4 | 11.3 | 9.7 | 12.2 | 12.2 | 13.6 | 16.9 | 16.6 | 19.6 | 13.1 | 21.9 | 27.8 | 13.4 | 13.0 | 13.5 | 6.6 | 5.7 | 8.1 |
Intensity Change (Log2) | – | – | – | 0.0 | 0.25 | 0.27 | 0.35 | 0.32 | 0.41 | 0.47 | 0.39 | 0.54 | 0.44 | 0.95 | 1.07 | 0.2 | 0.43 | 0.54 | -0.47 | -0.35 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0.7 | 1.0 | 0 | 1.0 | 1.8 | 2.1 | 2.4 | 2.5 | 0 | 0 | -0.5 | 0.4 | 1.4 | 1.1 |
Cytoplasm | 0.2 | -3.1 | -2.0 | -6.2 | -7.5 | -6.8 | -7.5 | -8.2 | -8.6 | 0.8 | 0.8 | 0.5 | -2.6 | -3.1 | -1.2 |
Endoplasmic Reticulum | 0 | 2.0 | 1.0 | 5.2 | 6.3 | 4.8 | 4.7 | 4.5 | 5.1 | 0 | 0 | 0 | 1.3 | 1.9 | 0.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 33.8124 | 44.4769 | 39.5281 | 36.5198 | 37.4122 | 41.5602 | 32.2716 | 43.6869 | 39.4567 | 40.2931 | 21.3442 | 37.6519 | 48.0105 | 51.2604 | 46.3155 | 40.9043 | 38.1426 | 48.1277 |
Actin | 0.027 | 0.0001 | 0.0064 | 0.0107 | 0.0054 | 0.0011 | 0.0127 | 0.0001 | 0.0006 | 0 | 0.0206 | 0.0091 | 0.0075 | 0.0038 | 0.0007 | 0.0038 | 0.0118 | 0.0003 |
Bud | 0.0015 | 0.0001 | 0.0003 | 0.0011 | 0.0021 | 0.0003 | 0.0011 | 0 | 0.0015 | 0.0001 | 0.0004 | 0.0012 | 0.0001 | 0.0002 | 0.0004 | 0.0012 | 0.0053 | 0.0001 |
Bud Neck | 0.0023 | 0.0002 | 0.0004 | 0.0007 | 0.0008 | 0.0015 | 0.0009 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0008 | 0.0003 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0004 |
Bud Periphery | 0.0017 | 0.0001 | 0.0004 | 0.0011 | 0.0019 | 0.0002 | 0.0022 | 0 | 0.0004 | 0 | 0.0004 | 0.001 | 0.0001 | 0.0002 | 0.0004 | 0.0011 | 0.0033 | 0 |
Bud Site | 0.0026 | 0.0001 | 0.0006 | 0.0004 | 0.0075 | 0.0001 | 0.0019 | 0 | 0.0011 | 0 | 0.0011 | 0.0009 | 0.0002 | 0.0003 | 0.0003 | 0.0015 | 0.0125 | 0 |
Cell Periphery | 0.0007 | 0.0002 | 0.0008 | 0.0007 | 0.0009 | 0.0003 | 0.0011 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0002 | 0 |
Cytoplasm | 0.7892 | 0.9864 | 0.9043 | 0.9034 | 0.805 | 0.9004 | 0.7904 | 0.9979 | 0.9239 | 0.9919 | 0.5911 | 0.9312 | 0.9332 | 0.9647 | 0.9289 | 0.8985 | 0.8619 | 0.987 |
Cytoplasmic Foci | 0.0299 | 0.0028 | 0.0137 | 0.0078 | 0.0244 | 0.0087 | 0.0146 | 0.0004 | 0.0183 | 0.0015 | 0.079 | 0.0069 | 0.0039 | 0.0036 | 0.0078 | 0.0155 | 0.0262 | 0.0016 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0003 | 0.0008 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.009 | 0.0017 | 0.0057 | 0.0086 | 0.0041 | 0.0082 | 0.0262 | 0.0002 | 0.0023 | 0.0004 | 0.0191 | 0.0016 | 0.006 | 0.0004 | 0.0082 | 0.0073 | 0.0041 | 0.006 |
Endosome | 0.014 | 0.0008 | 0.0073 | 0.0053 | 0.0215 | 0.0155 | 0.0272 | 0.0002 | 0.0124 | 0.0017 | 0.0999 | 0.0058 | 0.0171 | 0.0009 | 0.0065 | 0.0062 | 0.009 | 0.0012 |
Golgi | 0.003 | 0 | 0.0036 | 0.0008 | 0.0073 | 0.0021 | 0.0099 | 0 | 0.0017 | 0 | 0.0129 | 0.0046 | 0.0054 | 0.0018 | 0.0005 | 0.002 | 0.0044 | 0.0001 |
Lipid Particles | 0.0073 | 0.0001 | 0.0073 | 0.0023 | 0.0399 | 0.0026 | 0.004 | 0 | 0.0009 | 0 | 0.039 | 0.0021 | 0.0025 | 0.0096 | 0.0034 | 0.0013 | 0.0065 | 0 |
Mitochondria | 0.0108 | 0 | 0.0082 | 0.0013 | 0.0403 | 0.0184 | 0.0277 | 0 | 0.0198 | 0.0001 | 0.0035 | 0.0038 | 0.0037 | 0.0111 | 0.0082 | 0.049 | 0.0194 | 0.0001 |
None | 0.0106 | 0.0003 | 0.0029 | 0.0104 | 0.0034 | 0.0005 | 0.0112 | 0.0001 | 0.0043 | 0.0003 | 0.0083 | 0.0003 | 0.0008 | 0.0003 | 0.0082 | 0.0019 | 0.0075 | 0.0002 |
Nuclear Periphery | 0.0165 | 0.0011 | 0.0065 | 0.0053 | 0.0034 | 0.0054 | 0.032 | 0.0002 | 0.001 | 0.0008 | 0.0746 | 0.0013 | 0.0049 | 0.0004 | 0.0041 | 0.0011 | 0.0024 | 0.0009 |
Nucleolus | 0.0066 | 0 | 0.0011 | 0.0002 | 0.0056 | 0.004 | 0.0015 | 0 | 0.0003 | 0 | 0.0016 | 0.0211 | 0.0001 | 0 | 0.0024 | 0.0002 | 0.0008 | 0 |
Nucleus | 0.0142 | 0.0014 | 0.007 | 0.0053 | 0.0055 | 0.0046 | 0.0128 | 0.0004 | 0.0016 | 0.0014 | 0.0094 | 0.0037 | 0.0047 | 0.0007 | 0.0038 | 0.0012 | 0.0034 | 0.0012 |
Peroxisomes | 0.0026 | 0 | 0.0015 | 0.0022 | 0.003 | 0.0012 | 0.0008 | 0 | 0.0005 | 0 | 0.0083 | 0.0012 | 0.0008 | 0.0014 | 0.0019 | 0.0028 | 0.0094 | 0 |
Punctate Nuclear | 0.0197 | 0.0001 | 0.0017 | 0.0031 | 0.0008 | 0.0007 | 0.0045 | 0 | 0.0004 | 0 | 0.0199 | 0.0015 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0062 | 0.0001 |
Vacuole | 0.0223 | 0.004 | 0.0112 | 0.0213 | 0.0101 | 0.0163 | 0.0135 | 0.0003 | 0.0065 | 0.0012 | 0.0054 | 0.0012 | 0.007 | 0.0003 | 0.0089 | 0.0029 | 0.0037 | 0.0005 |
Vacuole Periphery | 0.0081 | 0.0005 | 0.009 | 0.0077 | 0.0063 | 0.008 | 0.0035 | 0.0001 | 0.0016 | 0.0003 | 0.0046 | 0.0005 | 0.0013 | 0.0002 | 0.0036 | 0.0013 | 0.0013 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 66.7382 | 45.7392 | 80.1782 | 140.3026 | 85.5675 | 131.1791 | 86.9642 | 128.4731 | 197.0533 | 106.8383 |
Translational Efficiency | 2.2837 | 2.2216 | 1.9841 | 2.104 | 2.2195 | 1.9921 | 1.9793 | 1.9436 | 1.7251 | 1.7764 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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