Standard name
Human Ortholog
Description Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0.06 0.07 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.98 0.98 0.92 0.95 0.82 0.77 0.79 0.76 0.72 0.71 0.99 0.99 0.99 0.93 0.93 0.96 0.95 0.96 0.88 0.93 0.91 0.92
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.13 0.17 0.12 0.12 0.11 0.13 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 4 5 9 6 11 9 0 0 0 0 0 0 0 2 1 0 3 0
Bud 0 0 0 0 0 0 2 1 1 0 2 1 0 0 1 1 0 2 0 0 1 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 9 7 3 8 11 7 13 15 14 13 18 3 2 6 8 14 12 0 0 1 0 0 0
Cytoplasm 232 254 260 126 197 246 220 234 202 164 125 169 197 284 291 230 260 269 230 247 241 153 217 144
Endoplasmic Reticulum 2 4 4 0 10 7 36 53 31 26 20 31 2 3 5 8 12 7 1 2 13 3 7 4
Endosome 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1
Golgi 0 0 0 0 1 1 1 3 6 4 2 7 0 0 0 3 5 0 0 0 3 0 1 1
Mitochondria 1 0 0 1 1 3 1 4 8 3 13 18 0 1 0 4 2 0 0 0 1 0 0 0
Nucleus 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 0 1 1 1 2 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 4 0 2 10 3 9 4 6 1 0 0 9 15 3 2 1 1 1 1 0
Unique Cell Count 234 259 265 128 214 259 268 305 255 216 174 238 199 287 295 246 281 279 242 258 273 166 238 156
Labelled Cell Count 237 267 273 130 221 270 276 324 276 227 191 261 203 290 304 263 310 293 242 258 273 166 238 156


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.9 36.4 30.9 31.0 36.7 37.4 39.5 38.7 41.0 42.8 40.4 44.8 41.8 59.6 64.8 35.6 41.7 45.1 22.4 24.2 28.3
Std Deviation (1e-4) 4.5 8.4 11.0 11.4 11.3 9.7 12.2 12.2 13.6 16.9 16.6 19.6 13.1 21.9 27.8 13.4 13.0 13.5 6.6 5.7 8.1
Intensity Change (Log2) 0.0 0.25 0.27 0.35 0.32 0.41 0.47 0.39 0.54 0.44 0.95 1.07 0.2 0.43 0.54 -0.47 -0.35 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.7 1.0 0 1.0 1.8 2.1 2.4 2.5 0 0 -0.5 0.4 1.4 1.1
Cytoplasm 0.2 -3.1 -2.0 -6.2 -7.5 -6.8 -7.5 -8.2 -8.6 0.8 0.8 0.5 -2.6 -3.1 -1.2
Endoplasmic Reticulum 0 2.0 1.0 5.2 6.3 4.8 4.7 4.5 5.1 0 0 0 1.3 1.9 0.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.5 4.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.5 0 0 0 0 0 0 0 0 3.4 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 33.8124 44.4769 39.5281 36.5198 37.4122 41.5602 32.2716 43.6869 39.4567 40.2931 21.3442 37.6519 48.0105 51.2604 46.3155 40.9043 38.1426 48.1277
Actin 0.027 0.0001 0.0064 0.0107 0.0054 0.0011 0.0127 0.0001 0.0006 0 0.0206 0.0091 0.0075 0.0038 0.0007 0.0038 0.0118 0.0003
Bud 0.0015 0.0001 0.0003 0.0011 0.0021 0.0003 0.0011 0 0.0015 0.0001 0.0004 0.0012 0.0001 0.0002 0.0004 0.0012 0.0053 0.0001
Bud Neck 0.0023 0.0002 0.0004 0.0007 0.0008 0.0015 0.0009 0.0001 0.0003 0.0002 0.0006 0.0008 0.0003 0.0001 0.0003 0.0005 0.0005 0.0004
Bud Periphery 0.0017 0.0001 0.0004 0.0011 0.0019 0.0002 0.0022 0 0.0004 0 0.0004 0.001 0.0001 0.0002 0.0004 0.0011 0.0033 0
Bud Site 0.0026 0.0001 0.0006 0.0004 0.0075 0.0001 0.0019 0 0.0011 0 0.0011 0.0009 0.0002 0.0003 0.0003 0.0015 0.0125 0
Cell Periphery 0.0007 0.0002 0.0008 0.0007 0.0009 0.0003 0.0011 0 0.0004 0 0.0001 0.0001 0.0001 0 0.0004 0.0002 0.0002 0
Cytoplasm 0.7892 0.9864 0.9043 0.9034 0.805 0.9004 0.7904 0.9979 0.9239 0.9919 0.5911 0.9312 0.9332 0.9647 0.9289 0.8985 0.8619 0.987
Cytoplasmic Foci 0.0299 0.0028 0.0137 0.0078 0.0244 0.0087 0.0146 0.0004 0.0183 0.0015 0.079 0.0069 0.0039 0.0036 0.0078 0.0155 0.0262 0.0016
Eisosomes 0.0006 0 0.0002 0.0003 0.0008 0 0.0003 0 0 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.009 0.0017 0.0057 0.0086 0.0041 0.0082 0.0262 0.0002 0.0023 0.0004 0.0191 0.0016 0.006 0.0004 0.0082 0.0073 0.0041 0.006
Endosome 0.014 0.0008 0.0073 0.0053 0.0215 0.0155 0.0272 0.0002 0.0124 0.0017 0.0999 0.0058 0.0171 0.0009 0.0065 0.0062 0.009 0.0012
Golgi 0.003 0 0.0036 0.0008 0.0073 0.0021 0.0099 0 0.0017 0 0.0129 0.0046 0.0054 0.0018 0.0005 0.002 0.0044 0.0001
Lipid Particles 0.0073 0.0001 0.0073 0.0023 0.0399 0.0026 0.004 0 0.0009 0 0.039 0.0021 0.0025 0.0096 0.0034 0.0013 0.0065 0
Mitochondria 0.0108 0 0.0082 0.0013 0.0403 0.0184 0.0277 0 0.0198 0.0001 0.0035 0.0038 0.0037 0.0111 0.0082 0.049 0.0194 0.0001
None 0.0106 0.0003 0.0029 0.0104 0.0034 0.0005 0.0112 0.0001 0.0043 0.0003 0.0083 0.0003 0.0008 0.0003 0.0082 0.0019 0.0075 0.0002
Nuclear Periphery 0.0165 0.0011 0.0065 0.0053 0.0034 0.0054 0.032 0.0002 0.001 0.0008 0.0746 0.0013 0.0049 0.0004 0.0041 0.0011 0.0024 0.0009
Nucleolus 0.0066 0 0.0011 0.0002 0.0056 0.004 0.0015 0 0.0003 0 0.0016 0.0211 0.0001 0 0.0024 0.0002 0.0008 0
Nucleus 0.0142 0.0014 0.007 0.0053 0.0055 0.0046 0.0128 0.0004 0.0016 0.0014 0.0094 0.0037 0.0047 0.0007 0.0038 0.0012 0.0034 0.0012
Peroxisomes 0.0026 0 0.0015 0.0022 0.003 0.0012 0.0008 0 0.0005 0 0.0083 0.0012 0.0008 0.0014 0.0019 0.0028 0.0094 0
Punctate Nuclear 0.0197 0.0001 0.0017 0.0031 0.0008 0.0007 0.0045 0 0.0004 0 0.0199 0.0015 0.0002 0.0001 0.0007 0.0001 0.0062 0.0001
Vacuole 0.0223 0.004 0.0112 0.0213 0.0101 0.0163 0.0135 0.0003 0.0065 0.0012 0.0054 0.0012 0.007 0.0003 0.0089 0.0029 0.0037 0.0005
Vacuole Periphery 0.0081 0.0005 0.009 0.0077 0.0063 0.008 0.0035 0.0001 0.0016 0.0003 0.0046 0.0005 0.0013 0.0002 0.0036 0.0013 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 66.7382 45.7392 80.1782 140.3026 85.5675 131.1791 86.9642 128.4731 197.0533 106.8383
Translational Efficiency 2.2837 2.2216 1.9841 2.104 2.2195 1.9921 1.9793 1.9436 1.7251 1.7764

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Tps1

Tps1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tps1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available