Standard name
Human Ortholog
Description Essential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 – 0.05 0.05 0.08 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.11 0 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.07 0.07 0.1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.13
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.16 0.2 – 0.28 0.38 0.37 0.22 0 0 0 0.11 0.09 0.06 0 0 0 0 0 0
Nucleus 0.4 0.33 0.28 0.09 0.2 0.2 0.21 – 0.18 0.21 0.25 0.16 0.07 0.09 0.08 0.27 0.28 0.32 0.22 0.22 0.18 0.07 0.08 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleolus 0.53 0.67 0.68 0.9 0.72 0.66 0.62 – 0.65 0.58 0.56 0.62 0.9 0.82 0.8 0.62 0.62 0.6 0.62 0.65 0.62 0.62 0.48 0.41
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.11 0.47 0.2 0.27 0.28 – 0.24 0.25 0.22 0.36 0.22 0.17 0.16 0.05 0.05 0.05 0 0 0.07 0.12 0.14 0.2
Vac/Vac Membrane 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.06 0.07 0 0.06 0 0.05 0.08 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 1 1 4 8 – 9 16 14 10 2 0 0 0 0 0 0 1 2 2 2 3
Bud Neck 0 1 0 6 4 1 5 – 1 4 4 5 4 3 1 0 0 0 0 0 1 2 8 9
Bud Site 0 0 0 1 1 6 25 – 23 22 34 21 1 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 5 6 9 10 20 – 7 13 8 5 14 37 39 12 16 8 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 1 – 0 0 0 0 0 1 0 4 2 2 0 0 0 0 0 0
Endosome 0 0 9 21 21 45 19 – 18 1 5 16 4 13 13 13 5 5 3 1 4 11 20 31
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0
Mitochondria 6 8 0 10 11 76 94 – 135 176 164 84 14 1 9 40 21 11 0 1 3 5 4 7
Nucleus 48 108 86 29 60 94 99 – 85 99 109 63 24 30 29 96 66 59 31 86 64 15 19 10
Nuclear Periphery 5 5 11 0 1 7 4 – 2 6 5 3 0 0 1 2 2 1 8 13 15 1 2 5
Nucleolus 64 219 206 290 218 314 291 – 306 272 247 240 291 268 275 221 145 110 87 249 219 128 115 96
Peroxisomes 0 1 0 3 1 4 4 – 3 2 0 1 5 8 6 3 3 0 0 0 0 2 3 2
SpindlePole 2 2 33 153 61 126 132 – 114 116 96 138 70 54 56 18 11 9 2 5 26 23 33 47
Vac/Vac Membrane 6 11 10 2 4 4 5 – 7 7 5 3 2 0 2 20 13 12 4 22 13 10 18 12
Unique Cell Count 121 328 304 323 301 473 470 474 466 439 385 322 327 343 354 233 182 141 384 356 206 241 234
Labelled Cell Count 132 358 360 522 392 691 707 710 734 691 589 431 415 431 431 284 217 141 384 356 206 241 234


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.1 13.3 10.8 9.4 8.4 6.5 6.3 – 5.5 5.0 4.8 5.3 10.6 9.8 9.2 16.2 18.1 15.7 10.7 9.9 10.6
Std Deviation (1e-4) 3.7 3.3 2.8 3.1 2.0 1.5 1.7 – 1.5 1.3 1.3 1.1 3.0 3.6 3.2 6.7 6.7 6.9 3.4 2.1 2.7
Intensity Change (Log2) – – – -0.2 -0.37 -0.73 -0.77 – -0.96 -1.11 -1.17 -1.01 -0.02 -0.14 -0.23 0.59 0.75 0.55 -0.01 -0.12 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 3.3 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 4.1 – 3.9 3.8 0 4.1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 1.1 0.5 2.0 – 0 1.0 0.2 0 2.0 4.9 4.9 1.4 3.1 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 2.1 2.3 3.5 0.8 – 0.6 0 0 0.8 -1.5 0.7 0.6 0.5 -0.6 -0.1
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 3.4 7.4 8.3 – 10.2 12.2 0 8.7 3.7 0 0 6.0 5.3 0
Nucleus -6.2 -2.4 -2.7 -2.3 – -3.4 -2.2 -1.1 -3.8 -6.8 -6.2 -6.6 -0.3 0 1.0
Nuclear Periphery -3.4 -2.9 -1.9 -2.7 – -3.4 -2.2 0 -2.6 -3.4 -3.5 -3.1 -2.8 -2.1 0
Nucleolus 6.8 1.3 -0.4 -1.7 – -0.9 -2.6 0 -1.5 7.0 4.1 3.6 -1.4 -1.3 -1.6
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 10.0 3.2 5.3 5.7 – 4.6 4.8 0 7.5 3.7 2.1 2.0 -2.8 -2.6 -2.2
Vacuole -2.4 -1.6 -2.5 -2.2 – -1.7 -1.6 -2.0 -2.4 -2.4 0 -2.5 1.4 1.3 1.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1765 6.8799 5.6606 5.4661 5.4851 5.8581 7.6968 11.8794 9.8155 9.0982 8.9857 9.193 7.2016 9.2579 8.1575 7.9347 7.4252 7.7763
Actin 0.0049 0 0.0112 0 0.0016 0.0074 0.0136 0 0.0061 0 0 0.0044 0.0114 0 0.0002 0.0025 0.0103 0.0005
Bud 0.0005 0.0001 0.0002 0 0.0005 0.0001 0.0004 0 0.0002 0.0001 0 0 0.0003 0 0 0.0002 0.0003 0.0001
Bud Neck 0.0016 0.0003 0.0005 0.0005 0.0012 0.0013 0.0005 0.0002 0.0071 0.0004 0.0001 0.0005 0.0006 0.0002 0.0001 0.0007 0.0011 0.0004
Bud Periphery 0.0019 0.0001 0.0004 0.0001 0.001 0.0003 0.0007 0.0001 0.0003 0.0002 0 0.0001 0.0005 0.0001 0 0.0004 0.0006 0.0002
Bud Site 0.0019 0.0003 0.0011 0.0001 0.0029 0.0002 0.002 0.0001 0.0009 0.0002 0.0001 0.0001 0.0008 0.0003 0.0001 0.0026 0.0021 0.0001
Cell Periphery 0.0032 0.0001 0.0006 0.0001 0.0002 0.0004 0.0005 0.0004 0.0002 0.001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0003 0.0003 0.0002
Cytoplasm 0.0035 0.0001 0.0029 0.0001 0.0015 0.0004 0.0056 0 0.003 0 0 0.0034 0.0005 0 0.0005 0.0002 0.0004 0.0007
Cytoplasmic Foci 0.0065 0.0004 0.0061 0.0011 0.0008 0.0017 0.0444 0.0004 0.0063 0.0002 0.0019 0.0029 0.0058 0.0002 0.0011 0.0068 0.0069 0.0034
Eisosomes 0.0066 0 0.0001 0 0.0001 0.0001 0.0002 0 0.0001 0 0 0 0.0001 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0076 0 0.0046 0 0.0019 0.0016 0.002 0 0.0025 0 0 0.0002 0.0004 0 0.0053 0.0003 0.0009 0.0009
Endosome 0.0163 0.0001 0.0073 0.0001 0.0095 0.0042 0.0229 0 0.0099 0.0001 0 0.0066 0.0088 0.0001 0.0005 0.0025 0.01 0.0012
Golgi 0.0026 0 0.0023 0.0001 0.0007 0.0012 0.0097 0 0.0053 0 0 0.0032 0.0029 0 0.0001 0.0015 0.0042 0.0007
Lipid Particles 0.0096 0.0011 0.0054 0.0079 0.0016 0.011 0.0502 0.0008 0.0043 0.0005 0.005 0.0106 0.0083 0.0009 0.0008 0.0129 0.0217 0.0126
Mitochondria 0.0102 0.0001 0.0163 0.0001 0.0031 0.014 0.0094 0.0003 0.0021 0 0.0138 0.0054 0.0028 0.0001 0.0001 0.0056 0.0046 0.0006
None 0.0093 0 0.0013 0 0.0009 0.0003 0.0042 0 0.0007 0 0 0.0003 0.0008 0 0 0.0004 0.0009 0.0004
Nuclear Periphery 0.0281 0.0116 0.0072 0.0162 0.0328 0.012 0.0195 0.0049 0.0071 0.0001 0.0001 0.006 0.0042 0.0053 0.0095 0.0014 0.029 0.01
Nucleolus 0.7173 0.7436 0.7622 0.7878 0.5903 0.7408 0.6845 0.8885 0.8479 0.9186 0.909 0.861 0.7856 0.8109 0.8584 0.7842 0.6846 0.8246
Nucleus 0.134 0.2241 0.1376 0.1728 0.3283 0.1835 0.0731 0.1 0.0824 0.0713 0.0646 0.0841 0.15 0.1759 0.1168 0.1563 0.1613 0.1299
Peroxisomes 0.0044 0 0.0011 0 0.0004 0.0004 0.008 0 0.002 0 0.0004 0.0003 0.0019 0 0 0.0034 0.0094 0.0038
Punctate Nuclear 0.0196 0.0171 0.0164 0.0107 0.0135 0.0147 0.0364 0.0017 0.01 0.0005 0.0025 0.0081 0.0105 0.0042 0.0058 0.0134 0.0475 0.0077
Vacuole 0.0058 0.0006 0.0089 0.0006 0.0047 0.0016 0.0054 0.0012 0.0009 0.0063 0.0005 0.0007 0.0021 0.0009 0.0002 0.0014 0.0017 0.001
Vacuole Periphery 0.0046 0.0003 0.0063 0.0016 0.0025 0.0027 0.0069 0.0012 0.0007 0.0005 0.0016 0.0018 0.0015 0.0006 0.0002 0.0029 0.0023 0.0011

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.1828 43.754 40.9782 33.0651 35.7345 28.9709 28.133 26.0902 23.9087 39.5631
Translational Efficiency 1.2338 1.1261 1.0675 1.0957 1.2336 1.2125 1.2399 1.0336 1.0063 0.9453

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
Localization
Cell Percentages nucleolus (51%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Mak5

Mak5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mak5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available