Standard name | |
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Human Ortholog | |
Description | Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 3 | 7 | 14 | 12 | 15 | 20 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 1 | 0 | 3 | 3 | 3 | 4 | 2 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 4 | 8 | 6 | 7 | 15 | 32 | 60 | 33 | 36 | 19 | 29 | 4 | 8 | 7 | 11 | 5 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 181 | 118 | 296 | 269 | 410 | 336 | 421 | 488 | 282 | 311 | 267 | 297 | 174 | 289 | 307 | 225 | 313 | 295 | 181 | 109 | 276 | 66 | 337 | 289 |
Endoplasmic Reticulum | 1 | 3 | 4 | 4 | 10 | 14 | 69 | 104 | 54 | 70 | 31 | 50 | 2 | 0 | 0 | 9 | 8 | 12 | 1 | 1 | 5 | 0 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 9 | 2 | 2 | 6 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 0 | 8 | 1 | 9 | 11 | 19 | 9 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 7 | 4 | 12 | 4 | 7 | 2 | 0 | 1 | 1 | 3 | 2 | 0 | 1 | 2 | 0 | 2 | 1 |
Unique Cell Count | 183 | 120 | 300 | 272 | 424 | 361 | 519 | 640 | 368 | 420 | 343 | 375 | 177 | 290 | 310 | 228 | 316 | 299 | 187 | 115 | 295 | 71 | 346 | 298 |
Labelled Cell Count | 184 | 125 | 309 | 280 | 428 | 372 | 545 | 690 | 401 | 460 | 370 | 416 | 182 | 298 | 317 | 247 | 331 | 322 | 187 | 115 | 295 | 71 | 346 | 298 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 50.8 | 69.0 | 54.6 | 48.9 | 40.4 | 33.7 | 30.4 | 29.8 | 27.6 | 26.0 | 24.9 | 24.7 | 63.1 | 66.8 | 59.3 | 68.3 | 70.9 | 71.9 | 67.1 | 65.4 | 65.9 |
Std Deviation (1e-4) | 10.6 | 15.0 | 9.6 | 9.7 | 7.4 | 7.2 | 6.5 | 6.4 | 5.6 | 5.3 | 5.0 | 5.0 | 15.3 | 14.0 | 14.4 | 24.0 | 23.9 | 23.0 | 17.4 | 13.0 | 15.5 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.44 | -0.7 | -0.84 | -0.88 | -0.98 | -1.07 | -1.13 | -1.14 | 0.21 | 0.29 | 0.12 | 0.32 | 0.38 | 0.4 | 0.3 | 0.26 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.3 | 4.2 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.4 | -0.9 | 1.0 | 2.2 | 3.7 | 3.4 | 3.3 | 1.8 | 2.9 | 0 | 0.1 | -0.3 | 1.3 | -0.9 | 0.9 |
Cytoplasm | 0.3 | -1.7 | -3.5 | -7.3 | -8.6 | -8.3 | -9.0 | -8.0 | -7.7 | -0.3 | 1.3 | 0.4 | 0 | 0.4 | 0 |
Endoplasmic Reticulum | 0 | 1.0 | 2.0 | 5.8 | 6.7 | 6.1 | 6.7 | 4.3 | 5.7 | 0 | 0 | 0 | 1.9 | 1.1 | 2.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | 0.033 | 0.0002 | 0.0098 | 0.0076 | 0.0193 | 0.0106 | 0.0035 | 0.0001 | 0.0028 | 0.0019 | 0.0037 | 0.0001 |
Bud | – | – | – | – | – | – | 0.0009 | 0.0001 | 0.0003 | 0.0032 | 0.0003 | 0.0006 | 0.0014 | 0.0001 | 0.0005 | 0.0015 | 0.0007 | 0.0001 |
Bud Neck | – | – | – | – | – | – | 0.0011 | 0.0003 | 0.0006 | 0.0013 | 0.0003 | 0.0012 | 0.0008 | 0.0001 | 0.0005 | 0.0035 | 0.0009 | 0.0004 |
Bud Periphery | – | – | – | – | – | – | 0.001 | 0 | 0.0002 | 0.0063 | 0.0007 | 0.0012 | 0.0017 | 0 | 0.0006 | 0.0015 | 0.0006 | 0 |
Bud Site | – | – | – | – | – | – | 0.0126 | 0.0002 | 0.0029 | 0.0047 | 0.0005 | 0.0011 | 0.0019 | 0 | 0.0015 | 0.0061 | 0.0005 | 0 |
Cell Periphery | – | – | – | – | – | – | 0.0006 | 0.0004 | 0.0004 | 0.0013 | 0.0001 | 0.001 | 0.0025 | 0.001 | 0.0006 | 0.0005 | 0.0003 | 0 |
Cytoplasm | – | – | – | – | – | – | 0.8847 | 0.9933 | 0.966 | 0.8605 | 0.7939 | 0.8587 | 0.8405 | 0.991 | 0.9439 | 0.9411 | 0.8936 | 0.9966 |
Cytoplasmic Foci | – | – | – | – | – | – | 0.0096 | 0.0006 | 0.0064 | 0.0037 | 0.0065 | 0.0014 | 0.0238 | 0.0005 | 0.0077 | 0.0051 | 0.005 | 0.0003 |
Eisosomes | – | – | – | – | – | – | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | – | – | – | – | – | – | 0.0084 | 0.0006 | 0.001 | 0.0045 | 0.0028 | 0.0058 | 0.0055 | 0.0007 | 0.0007 | 0.0002 | 0.0031 | 0.0002 |
Endosome | – | – | – | – | – | – | 0.0038 | 0.0001 | 0.0006 | 0.0332 | 0.0468 | 0.018 | 0.0205 | 0.0001 | 0.0121 | 0.0053 | 0.0372 | 0.0002 |
Golgi | – | – | – | – | – | – | 0.0029 | 0 | 0.0005 | 0.0145 | 0.0252 | 0.0204 | 0.0042 | 0 | 0.0029 | 0.0022 | 0.0241 | 0 |
Lipid Particles | – | – | – | – | – | – | 0.0032 | 0.0001 | 0.0021 | 0.0036 | 0.0365 | 0.0022 | 0.0123 | 0.0004 | 0.0019 | 0.0014 | 0.0034 | 0 |
Mitochondria | – | – | – | – | – | – | 0.0021 | 0 | 0.002 | 0.0193 | 0.0118 | 0.0041 | 0.0117 | 0.0002 | 0.0046 | 0.0082 | 0.0068 | 0 |
None | – | – | – | – | – | – | 0.0024 | 0.0013 | 0.0017 | 0.0005 | 0.0015 | 0.0008 | 0.01 | 0.0014 | 0.001 | 0.0081 | 0.0005 | 0.0001 |
Nuclear Periphery | – | – | – | – | – | – | 0.0086 | 0.0005 | 0.0009 | 0.0041 | 0.031 | 0.026 | 0.014 | 0.0002 | 0.0017 | 0.0004 | 0.0025 | 0.0005 |
Nucleolus | – | – | – | – | – | – | 0.0007 | 0.0001 | 0.0006 | 0.0006 | 0.0005 | 0.0106 | 0.0042 | 0.0013 | 0.0053 | 0.0033 | 0.0001 | 0 |
Nucleus | – | – | – | – | – | – | 0.0087 | 0.001 | 0.0014 | 0.0031 | 0.003 | 0.0208 | 0.007 | 0.0011 | 0.0043 | 0.0026 | 0.0015 | 0.0011 |
Peroxisomes | – | – | – | – | – | – | 0.0015 | 0 | 0.0005 | 0.0007 | 0.0049 | 0.0002 | 0.001 | 0 | 0.0015 | 0.0014 | 0.0002 | 0 |
Punctate Nuclear | – | – | – | – | – | – | 0.0081 | 0.0001 | 0.0004 | 0.0004 | 0.0073 | 0.0024 | 0.0085 | 0 | 0.0006 | 0.0003 | 0.0002 | 0 |
Vacuole | – | – | – | – | – | – | 0.0054 | 0.001 | 0.001 | 0.0225 | 0.0033 | 0.0073 | 0.0171 | 0.0016 | 0.004 | 0.0039 | 0.0113 | 0.0004 |
Vacuole Periphery | – | – | – | – | – | – | 0.0007 | 0.0001 | 0.0004 | 0.0044 | 0.0036 | 0.0055 | 0.0072 | 0.0002 | 0.0015 | 0.0012 | 0.0039 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 225.4673 | 221.4467 | 211.1133 | 229.2691 | 191.0749 | 178.7633 | 201.1346 | 187.748 | 179.5606 | 197.8185 |
Translational Efficiency | 1.4447 | 1.475 | 1.4097 | 1.2724 | 1.3225 | 1.6042 | 1.3878 | 1.2516 | 1.2641 | 1.3594 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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