Standard name
Human Ortholog
Description Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.99 0.98 0.93 0.9 0.84 0.85 0.87 0.86 0.82 0.98 0.99 1.0 0.94 0.94 0.94 0.98 0.94 0.9 0.94 0.91 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0.05 0.07 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 3 5 4 3 2 5 0 0 0 0 0 2 0 2 0 0 0 3
Bud 0 0 2 0 0 2 2 2 3 2 1 1 0 0 0 0 0 0 1 1 1 0 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 5 1 6 11 14 10 9 8 10 18 1 1 4 7 7 3 0 0 0 0 0 0
Cytoplasm 263 283 172 193 269 264 318 299 189 303 230 226 125 124 149 274 227 210 249 265 157 105 136 137
Endoplasmic Reticulum 0 0 1 1 2 12 20 29 10 12 2 6 1 3 0 8 14 7 0 2 1 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10
Golgi 0 1 2 1 0 0 0 1 0 0 0 0 1 0 0 16 12 7 0 0 1 0 0 4
Mitochondria 0 0 0 0 0 0 4 0 6 8 18 13 0 0 0 0 1 0 0 1 1 0 0 1
Nucleus 0 0 0 0 1 0 3 4 3 3 1 1 0 0 0 0 0 1 0 3 1 0 1 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 1 0 0 0 1 1 1 0 0 1 0 0 0 4 4 4 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 2 6
Vac/Vac Membrane 0 1 1 0 1 3 8 17 12 24 17 31 2 0 0 10 3 0 0 3 3 1 1 6
Unique Cell Count 263 285 177 195 274 284 353 354 223 347 269 277 127 125 149 292 241 223 255 283 175 112 151 185
Labelled Cell Count 265 288 184 196 279 293 373 368 237 364 281 302 130 128 153 319 270 235 255 283 175 112 151 185


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 39.2 53.1 36.7 39.2 34.1 26.2 21.2 17.9 14.9 13.3 12.7 11.5 49.4 44.8 48.9 65.4 79.3 80.7 39.2 32.8 33.3
Std Deviation (1e-4) 9.4 12.6 11.1 11.6 9.2 6.0 5.5 4.6 3.9 3.7 3.8 3.6 14.9 15.1 13.2 19.5 24.3 21.8 8.8 11.7 11.8
Intensity Change (Log2) – – – 0.09 -0.11 -0.49 -0.8 -1.03 -1.31 -1.46 -1.54 -1.67 0.43 0.29 0.41 0.83 1.11 1.14 0.09 -0.17 -0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.6 0.7 0 0.7 0 0.5 1.7 0 0 0 0 0 0
Cytoplasm 1.3 0.7 -1.9 -2.9 -4.4 -4.2 -3.7 -4.0 -4.9 0.7 1.2 2.1 -1.6 -1.4 -1.4
Endoplasmic Reticulum 0 0 0 2.8 3.6 0 0 0 0 0 0 0 0 2.8 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 2.4 2.2 0
Mitochondria 0 0 0 0 0 0 0 3.5 2.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.5 2.7 3.2 3.0 4.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 61.4861 80.6426 68.2811 67.4917 47.168 68.2866 54.1349 71.9667 65.5866 59.8872 49.3224 65.9016 51.4572 74.9587 69.755 69.0528 65.8518 67.7897
Actin 0.0041 0 0.0023 0.0003 0.0089 0.0041 0.0024 0.0001 0.0009 0.0001 0.0015 0.0001 0.0151 0.0001 0.0037 0 0.0001 0.0003
Bud 0.0006 0 0.0005 0.0001 0.0008 0.0015 0.0005 0.0008 0.0002 0.0001 0.0007 0.0002 0.0007 0 0.0005 0.001 0 0.0003
Bud Neck 0.0004 0.0001 0.0003 0.0001 0.0005 0.0008 0.0003 0.0001 0.0002 0.0002 0.0005 0.0008 0.0009 0 0.0003 0.0003 0.0002 0.0007
Bud Periphery 0.0004 0 0.0004 0.0001 0.0014 0.0017 0.0004 0.0002 0.0005 0 0.0005 0.0001 0.0007 0 0.0004 0.0002 0 0.0001
Bud Site 0.0009 0 0.0005 0.0001 0.001 0.0009 0.0007 0.0001 0.0004 0.0002 0.0007 0.0001 0.0011 0 0.0023 0.0001 0 0
Cell Periphery 0.0001 0 0.0001 0.0001 0.0004 0.0003 0.0002 0.0011 0.0004 0.0001 0.001 0.0001 0.0001 0 0.0003 0.0001 0 0.0001
Cytoplasm 0.7559 0.7466 0.6978 0.6785 0.5015 0.71 0.9332 0.9883 0.9655 0.9534 0.7653 0.9529 0.8696 0.9965 0.9526 0.9551 0.993 0.9689
Cytoplasmic Foci 0.0053 0.0001 0.0028 0.0096 0.0191 0.0089 0.0075 0.0002 0.0062 0.0153 0.0202 0.0015 0.011 0.0001 0.0062 0.0214 0.0001 0.0006
Eisosomes 0 0 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0.0004 0 0.0003 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0046 0.0003 0.0015 0.0008 0.0117 0.0025 0.0015 0.0004 0.0016 0.0006 0.0081 0.0007 0.0021 0.0001 0.001 0.0001 0.0001 0.0006
Endosome 0.0082 0 0.0071 0.003 0.0166 0.0142 0.0029 0.0002 0.004 0.001 0.0151 0.0021 0.0159 0.0001 0.0066 0.0018 0.0001 0.0022
Golgi 0.0008 0 0.0018 0.0003 0.0039 0.0034 0.0007 0 0.0008 0.0001 0.001 0.0002 0.0072 0 0.0015 0.0001 0 0
Lipid Particles 0.001 0 0.0004 0.0018 0.0078 0.0021 0.0056 0.0001 0.0016 0.0022 0.0112 0.0007 0.0157 0 0.0026 0.0007 0 0
Mitochondria 0.0023 0 0.0025 0.001 0.0901 0.0133 0.005 0.0001 0.0015 0.0014 0.0159 0.0005 0.0054 0 0.0016 0.0011 0 0.0002
None 0.0021 0.0001 0.0019 0.0067 0.0052 0.0047 0.0061 0.0002 0.0014 0.0043 0.0193 0.0007 0.0134 0.0001 0.0046 0.0011 0 0.0002
Nuclear Periphery 0.0297 0.0146 0.019 0.0179 0.0974 0.0138 0.0036 0.002 0.0018 0.0022 0.0326 0.0053 0.0074 0.0007 0.0013 0.0005 0.0018 0.001
Nucleolus 0.0007 0.0001 0.0004 0.0005 0.0031 0.0075 0.0032 0.0001 0.001 0.0037 0.0099 0.0034 0.0007 0 0.0026 0.0011 0 0
Nucleus 0.1746 0.2376 0.2574 0.2751 0.1997 0.192 0.0146 0.0043 0.0073 0.0089 0.0395 0.0182 0.0194 0.002 0.0059 0.0043 0.0041 0.0047
Peroxisomes 0.0004 0 0.0004 0.0002 0.0016 0.0005 0.0041 0 0.0006 0.0003 0.0013 0.0001 0.007 0 0.0007 0.0003 0 0
Punctate Nuclear 0.0034 0.0002 0.0008 0.0011 0.0171 0.0027 0.0027 0.0001 0.0008 0.0021 0.0266 0.0045 0.0034 0 0.0006 0.0004 0 0.0001
Vacuole 0.0028 0.0001 0.0012 0.0018 0.005 0.0096 0.0036 0.0011 0.0025 0.0024 0.0155 0.0048 0.0017 0.0002 0.0036 0.0069 0.0002 0.0146
Vacuole Periphery 0.0016 0.0001 0.0007 0.0009 0.0071 0.0053 0.001 0.0003 0.0008 0.0013 0.0131 0.003 0.0012 0.0001 0.001 0.0032 0.0001 0.0052

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.9876 18.6814 15.2486 12.5322 13.5475 60.5701 56.43 48.126 43.4217 38.6084
Translational Efficiency 1.7338 1.2853 0.7618 1.7252 1.3568 2.0235 1.765 1.607 1.3944 1.2025

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Adh5

Adh5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Adh5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available