Standard name | |
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Human Ortholog | |
Description | NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 1 | 3 | 1 | 4 | 3 | 5 | 2 | 5 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | – | 0 | 2 | 1 | 5 | 2 | 4 | 8 | 6 | 6 | 0 | 2 | 2 | 0 | 0 | 0 | 4 | 3 | 0 | 8 | 9 | 23 |
Bud Neck | 0 | 0 | – | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 1 | 6 | 1 | 6 | 4 | 7 | 8 | 5 | 14 | 1 | 0 | 0 | 15 | 4 | 10 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 232 | 72 | – | 143 | 314 | 246 | 319 | 244 | 236 | 247 | 168 | 215 | 123 | 175 | 175 | 258 | 145 | 250 | 217 | 77 | 32 | 150 | 168 | 174 |
Endoplasmic Reticulum | 3 | 1 | – | 0 | 2 | 3 | 7 | 10 | 1 | 11 | 5 | 4 | 0 | 1 | 0 | 3 | 5 | 15 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Golgi | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 20 | 1 | – | 2 | 3 | 29 | 26 | 23 | 52 | 35 | 40 | 12 | 0 | 0 | 0 | 0 | 0 | 1 | 8 | 0 | 0 | 2 | 2 | 4 |
Nucleus | 1 | 0 | – | 0 | 1 | 3 | 6 | 8 | 10 | 4 | 5 | 3 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | – | 0 | 0 | 2 | 4 | 1 | 4 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 0 | 1 | 0 | 1 | 2 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 3 | 4 |
Vac/Vac Membrane | 0 | 0 | – | 0 | 16 | 25 | 39 | 46 | 32 | 63 | 20 | 30 | 0 | 5 | 5 | 4 | 2 | 2 | 1 | 0 | 1 | 4 | 2 | 2 |
Unique Cell Count | 248 | 75 | 143 | 335 | 292 | 386 | 326 | 307 | 351 | 224 | 269 | 124 | 182 | 181 | 266 | 149 | 259 | 249 | 83 | 38 | 174 | 195 | 224 | |
Labelled Cell Count | 259 | 75 | 146 | 349 | 314 | 416 | 347 | 351 | 386 | 255 | 293 | 124 | 185 | 184 | 282 | 156 | 279 | 249 | 83 | 38 | 174 | 195 | 224 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 7.2 | – | 5.0 | 7.8 | 7.8 | 9.3 | 11.4 | 10.8 | 12.1 | 12.4 | 14.9 | 6.1 | 9.2 | 9.7 | 28.6 | 30.5 | 30.4 | 10.2 | 7.7 | 10.9 |
Std Deviation (1e-4) | 1.6 | 2.9 | – | 1.3 | 3.5 | 4.4 | 5.2 | 6.0 | 5.9 | 6.5 | 6.0 | 7.5 | 2.0 | 5.7 | 5.6 | 6.9 | 8.3 | 9.8 | 12.1 | 6.4 | 10.5 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.4032 | 6.2533 | 6.5849 | 8.0732 | 6.1293 | 6.2026 | 7.6485 | 9.9062 | 8.2804 | 7.6818 | 8.0667 | 7.7651 | 5.308 | 7.0352 | 4.0324 | 3.8824 | 3.103 | 6.8438 |
Actin | 0.0331 | 0.0057 | 0.0022 | 0.004 | 0.0007 | 0.0019 | 0.0269 | 0.0018 | 0.0111 | 0.0086 | 0.0339 | 0.0073 | 0.0293 | 0.002 | 0.0532 | 0.0053 | 0.0045 | 0.0081 |
Bud | 0.002 | 0.0028 | 0.0017 | 0.0007 | 0.0007 | 0.0007 | 0.0008 | 0.0107 | 0.0021 | 0.0025 | 0.0005 | 0.0015 | 0.0004 | 0.0067 | 0.0008 | 0.0004 | 0.0002 | 0.0004 |
Bud Neck | 0.0038 | 0.0005 | 0.0009 | 0.002 | 0.0005 | 0.0011 | 0.0025 | 0.0004 | 0.0005 | 0.001 | 0.0009 | 0.0018 | 0.0016 | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0007 |
Bud Periphery | 0.0027 | 0.0014 | 0.0019 | 0.0004 | 0.0003 | 0.0004 | 0.0011 | 0.0036 | 0.0016 | 0.0011 | 0.0004 | 0.002 | 0.0004 | 0.0022 | 0.0004 | 0.0009 | 0.0002 | 0.0006 |
Bud Site | 0.0052 | 0.015 | 0.0016 | 0.0007 | 0.0002 | 0.0003 | 0.0033 | 0.0075 | 0.0015 | 0.0005 | 0.0011 | 0.0005 | 0.0018 | 0.0036 | 0.0047 | 0.0002 | 0.0004 | 0.0004 |
Cell Periphery | 0.0004 | 0.0003 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.72 | 0.8626 | 0.838 | 0.7566 | 0.9502 | 0.8539 | 0.7601 | 0.9028 | 0.8514 | 0.8818 | 0.7739 | 0.8848 | 0.7408 | 0.905 | 0.7713 | 0.7315 | 0.7841 | 0.9259 |
Cytoplasmic Foci | 0.0374 | 0.0079 | 0.0165 | 0.0476 | 0.0076 | 0.0242 | 0.023 | 0.0144 | 0.0153 | 0.0264 | 0.0442 | 0.0075 | 0.0128 | 0.0087 | 0.0236 | 0.0042 | 0.0124 | 0.0025 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0.0003 | 0 | 0.0008 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0045 | 0.0015 | 0.0025 | 0.0009 | 0.0035 | 0.0022 | 0.0069 | 0.0013 | 0.002 | 0.0039 | 0.0074 | 0.0038 | 0.0214 | 0.0015 | 0.002 | 0.0036 | 0.0022 | 0.0023 |
Endosome | 0.0427 | 0.0016 | 0.0148 | 0.042 | 0.0035 | 0.0095 | 0.0297 | 0.0023 | 0.0091 | 0.0153 | 0.015 | 0.013 | 0.0252 | 0.0055 | 0.0139 | 0.0545 | 0.0607 | 0.0082 |
Golgi | 0.0135 | 0.001 | 0.0035 | 0.0145 | 0.0002 | 0.0046 | 0.0073 | 0.0001 | 0.0039 | 0.0033 | 0.0034 | 0.0031 | 0.0068 | 0.001 | 0.0064 | 0.0287 | 0.0595 | 0.0041 |
Lipid Particles | 0.0083 | 0.0001 | 0.0016 | 0.0037 | 0.0001 | 0.0007 | 0.0169 | 0.0001 | 0.0019 | 0.0082 | 0.0099 | 0.0047 | 0.0107 | 0.0046 | 0.0048 | 0.0062 | 0.0405 | 0.0011 |
Mitochondria | 0.0171 | 0.0004 | 0.0048 | 0.0013 | 0.0002 | 0.0091 | 0.0026 | 0.0003 | 0.001 | 0.0004 | 0.0023 | 0.0022 | 0.0054 | 0.0011 | 0.0039 | 0.0464 | 0.0186 | 0.0189 |
None | 0.0763 | 0.0916 | 0.0815 | 0.0918 | 0.0202 | 0.075 | 0.0624 | 0.0439 | 0.0676 | 0.0159 | 0.0794 | 0.0278 | 0.0796 | 0.0512 | 0.0926 | 0.0943 | 0.0049 | 0.0142 |
Nuclear Periphery | 0.0056 | 0.001 | 0.0023 | 0.0013 | 0.0016 | 0.0013 | 0.0099 | 0.0014 | 0.0022 | 0.005 | 0.004 | 0.012 | 0.0326 | 0.0008 | 0.0031 | 0.0019 | 0.0017 | 0.0025 |
Nucleolus | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0009 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0066 | 0.0038 | 0.0074 | 0.0192 | 0.0081 | 0.0076 | 0.0276 | 0.0065 | 0.0201 | 0.0127 | 0.0075 | 0.0183 | 0.0179 | 0.0024 | 0.0065 | 0.0105 | 0.0019 | 0.0073 |
Peroxisomes | 0.01 | 0.0008 | 0.0114 | 0.0103 | 0.0001 | 0.0049 | 0.0077 | 0.0002 | 0.0052 | 0.0039 | 0.007 | 0.0019 | 0.0041 | 0.0013 | 0.0018 | 0.0002 | 0.002 | 0.0003 |
Punctate Nuclear | 0.0061 | 0.0013 | 0.0036 | 0.0014 | 0.001 | 0.0012 | 0.0068 | 0.0013 | 0.0018 | 0.0079 | 0.0043 | 0.0044 | 0.0045 | 0.0006 | 0.0082 | 0.0003 | 0.0008 | 0.0004 |
Vacuole | 0.0032 | 0.0007 | 0.0023 | 0.0011 | 0.001 | 0.0009 | 0.0026 | 0.001 | 0.0013 | 0.001 | 0.0031 | 0.0015 | 0.0017 | 0.0012 | 0.0009 | 0.0054 | 0.002 | 0.0011 |
Vacuole Periphery | 0.001 | 0.0001 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0008 | 0.0001 | 0.0002 | 0.0003 | 0.0011 | 0.0008 | 0.0015 | 0.0002 | 0.0005 | 0.0047 | 0.0029 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 149.2528 | 90.5369 | 70.011 | 124.7041 | 76.714 | 152.3667 | 115.8727 | 137.5519 | 141.3953 | 130.0777 |
Translational Efficiency | 1.7301 | 1.8952 | 2.2565 | 1.5947 | 2.1222 | 2.0251 | 1.728 | 1.6018 | 1.8288 | 1.5992 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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