Standard name
Human Ortholog
Description TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70); stimulates ATPase activity of Ssa1p; ts mutants reduce Hsp82p function, overexpression suppresses phenotypes of HSP82 ts allele and cpr7 deletion; human homolog TTC4 complements yeast cns1 mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0 0.07 0.07 0.07 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.96 0.96 0.95 0.87 0.76 0.73 0.71 0.64 0.63 0.6 1.0 0.99 1.0 0.97 0.97 0.97 0.95 0.97 0.89 0.96 0.95 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.2 0.29 0.26 0.26 0.39 0.42 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.08 0.09 0.06 0.1 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 1 1 1 1
Bud 0 0 3 5 1 2 8 5 5 4 7 9 1 0 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1 2 22 5 5 4 16 11 3 17 13 18 7 5 2 13 17 8 0 0 0 0 0 0
Cytoplasm 239 173 336 278 316 301 345 358 229 293 260 231 364 530 575 182 225 119 231 164 318 302 398 415
Endoplasmic Reticulum 1 0 12 2 2 4 7 7 1 13 1 3 0 4 2 6 10 5 2 1 18 0 3 5
Endosome 0 0 0 0 0 1 0 2 1 0 0 0 1 0 0 0 1 0 0 0 1 1 2 5
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 2
Mitochondria 1 1 4 13 14 68 132 126 84 178 175 158 0 0 0 0 2 0 0 0 5 0 0 2
Nucleus 2 0 0 0 3 0 1 2 6 0 4 1 0 2 2 2 2 0 0 0 0 2 1 2
Nuclear Periphery 0 0 0 0 0 1 0 1 3 0 3 4 0 0 0 0 0 0 2 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 1 1 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 1 0 0 4 4 9 12 37 29 29 42 28 1 1 0 4 1 4 2 1 4 2 5 7
Unique Cell Count 244 174 351 289 334 347 451 488 321 457 412 388 365 537 577 188 233 123 243 169 359 316 419 453
Labelled Cell Count 245 176 378 309 345 390 522 551 362 535 505 455 374 542 581 209 258 136 243 169 359 316 419 453


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 13.9 11.4 10.4 9.1 7.9 7.4 7.2 6.4 6.6 6.3 6.3 12.0 11.8 10.7 21.6 22.4 22.0 12.4 12.6 12.5
Std Deviation (1e-4) 1.5 1.9 1.6 1.8 1.7 1.5 1.4 1.4 1.8 1.6 1.3 1.6 1.9 1.6 1.6 6.0 5.3 4.7 2.0 2.1 2.2
Intensity Change (Log2) -0.13 -0.33 -0.54 -0.63 -0.66 -0.83 -0.79 -0.86 -0.87 0.07 0.05 -0.09 0.92 0.97 0.94 0.11 0.14 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.8 -3.2 -3.6 -1.8 -3.0 -3.6 -1.7 -2.0 -1.0 -3.0 -4.5 -5.5 0.3 0.5 0.1
Cytoplasm 0.3 -0.7 -4.2 -7.5 -8.5 -8.6 -10.7 -10.9 -11.6 3.6 2.8 4.3 0.6 0.5 0.5
Endoplasmic Reticulum -2.3 -2.6 -2.0 -1.7 -1.9 -2.9 -0.5 -3.4 -2.5 -3.6 -2.9 -3.7 -0.1 0.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 2.5 8.0 10.5 9.7 9.6 12.8 13.4 13.0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 5.3 5.8 4.8 6.2 5.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.5895 8.3172 7.1529 5.9739 6.6452 7.312 6.7653 9.7179 8.8193 7.5691 7.5862 9.15 6.3341 9.4972 7.9604 6.3604 6.844 7.455
Actin 0.0064 0.0073 0.0005 0.0001 0.0043 0.0004 0.0235 0.0016 0.0101 0.0093 0.0134 0.0016 0.0077 0.0009 0.0077 0.0314 0.0066 0.0016
Bud 0.0002 0.0003 0.0002 0.0002 0.0281 0.0003 0.0012 0.0003 0.0006 0.0009 0.001 0.0004 0.0006 0.0027 0.0002 0.0012 0.0003 0.0013
Bud Neck 0.0002 0.0002 0.0003 0.0003 0.0004 0.0008 0.003 0.0003 0.0011 0.0007 0.0006 0.0008 0.0007 0.0002 0.0004 0.0012 0.0005 0.0021
Bud Periphery 0.0001 0.0001 0 0 0.0146 0.0003 0.0013 0.0003 0.0005 0.0011 0.0007 0.0004 0.0007 0.0007 0.0001 0.0015 0.0003 0.0007
Bud Site 0.0004 0.0006 0.0002 0.0001 0.0057 0.0001 0.006 0.0015 0.0073 0.0045 0.0067 0.0003 0.0019 0.0034 0.0002 0.0052 0.0003 0.0005
Cell Periphery 0.0001 0 0 0 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0 0.0002 0.0002 0 0.0003 0.0001 0.0001
Cytoplasm 0.9233 0.9722 0.9607 0.9139 0.915 0.9316 0.7255 0.9665 0.9344 0.8173 0.9001 0.9542 0.8849 0.982 0.9515 0.7923 0.8899 0.9466
Cytoplasmic Foci 0.0141 0.0066 0.0091 0.0246 0.0129 0.0143 0.039 0.0075 0.0093 0.0147 0.0094 0.0112 0.0219 0.0029 0.0045 0.0218 0.0148 0.0118
Eisosomes 0 0 0 0 0 0 0.0003 0 0 0.0001 0.0001 0 0.0002 0 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.0068 0.0013 0.004 0.0017 0.0028 0.0037 0.0112 0.0015 0.0029 0.0097 0.0011 0.0034 0.0096 0.0011 0.0029 0.0027 0.0025 0.0036
Endosome 0.019 0.0056 0.0175 0.0429 0.0086 0.0218 0.0796 0.0045 0.0091 0.0548 0.0108 0.012 0.0289 0.0017 0.0158 0.0409 0.0161 0.016
Golgi 0.0026 0.0008 0.0014 0.0013 0.0005 0.0017 0.0209 0.0018 0.0021 0.0181 0.0072 0.0018 0.0071 0.0001 0.0078 0.0211 0.0098 0.0022
Lipid Particles 0.0003 0 0 0.0001 0.0001 0.0001 0.0093 0.0004 0.0028 0.0038 0.0007 0.0013 0.0038 0 0.0014 0.0199 0.0051 0.0012
Mitochondria 0.0005 0.0003 0.0004 0.0005 0.0007 0.009 0.0226 0.0078 0.0008 0.039 0.0407 0.0024 0.008 0.0001 0.003 0.0199 0.0187 0.0019
None 0.0148 0.0017 0.0012 0.0075 0.0018 0.0106 0.0072 0.0012 0.0085 0.0025 0.0013 0.0013 0.0064 0.0022 0.0011 0.0013 0.0013 0.0012
Nuclear Periphery 0.0014 0.0005 0.0006 0.0005 0.0007 0.0005 0.0093 0.0005 0.0009 0.008 0.0005 0.0015 0.0023 0.0003 0.0008 0.0053 0.0012 0.0014
Nucleolus 0 0 0 0 0.0001 0 0.0005 0 0.0001 0.0002 0 0.0004 0.0005 0 0 0.0005 0 0.0005
Nucleus 0.0057 0.0009 0.0011 0.001 0.0012 0.0011 0.0115 0.0011 0.0015 0.0036 0.0019 0.0028 0.0043 0.0008 0.0009 0.0026 0.0018 0.0017
Peroxisomes 0.0008 0.0001 0.0001 0.0002 0.0004 0.0001 0.012 0.0017 0.0054 0.0013 0.0016 0.0012 0.0041 0 0.0004 0.0114 0.0217 0.0007
Punctate Nuclear 0.0003 0.0001 0.0001 0.0002 0.0004 0.0014 0.0067 0.0001 0.0007 0.0011 0.0003 0.0008 0.0014 0.0002 0.0002 0.0117 0.0068 0.0018
Vacuole 0.0024 0.0011 0.0015 0.0036 0.0013 0.0015 0.0069 0.0009 0.0014 0.006 0.0013 0.0015 0.0038 0.0006 0.0007 0.0054 0.0018 0.0023
Vacuole Periphery 0.0005 0.0003 0.0007 0.0011 0.0004 0.0007 0.0023 0.0002 0.0003 0.0029 0.0005 0.0006 0.001 0.0001 0.0004 0.0021 0.0007 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.5906 29.8128 32.8675 27.1797 41.3864 19.0465 21.3159 27.611 24.1223 32.0668
Translational Efficiency 2.635 2.7202 2.2075 2.0362 1.7838 3.4716 2.2625 1.6345 1.9344 1.9817

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70); stimulates ATPase activity of Ssa1p; ts mutants reduce Hsp82p function, overexpression suppresses phenotypes of HSP82 ts allele and cpr7 deletion; human homolog TTC4 complements yeast cns1 mutant
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Cns1

Cns1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cns1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available