Standard name | |
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Human Ortholog | |
Description | Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 3 | 13 | 14 | 27 | 22 | 32 | 39 | – | 33 | 30 | 36 | 1 | 5 | 10 | 16 | 10 | 7 | 0 | 2 | 1 | 2 | 2 | 3 |
Bud | 1 | 0 | 1 | 0 | 9 | 11 | 3 | 11 | – | 5 | 16 | 12 | 0 | 1 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 8 | 9 | 5 | 9 | 17 | 13 | 26 | 15 | – | 27 | 30 | 39 | 9 | 16 | 42 | 51 | 33 | 39 | 0 | 0 | 1 | 0 | 0 | 1 |
Cytoplasm | 20 | 26 | 14 | 22 | 33 | 38 | 49 | 67 | – | 87 | 64 | 65 | 14 | 9 | 13 | 61 | 35 | 45 | 0 | 4 | 2 | 2 | 4 | 6 |
Endoplasmic Reticulum | 203 | 141 | 85 | 93 | 149 | 112 | 215 | 95 | – | 171 | 76 | 135 | 73 | 140 | 174 | 3 | 1 | 2 | 222 | 152 | 99 | 152 | 174 | 156 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 |
Golgi | 2 | 1 | 3 | 15 | 4 | 0 | 0 | 3 | – | 1 | 0 | 1 | 1 | 3 | 1 | 21 | 16 | 9 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 1 | 2 | 7 | 0 | 1 | 14 | 10 | – | 15 | 56 | 49 | 0 | 0 | 0 | 5 | 6 | 2 | 0 | 0 | 5 | 2 | 2 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | – | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 0 | 3 | 4 | 0 | 2 | 14 | 3 | – | 14 | 1 | 10 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 3 | 3 | 3 | 18 | 15 | 6 | 47 | – | 22 | 18 | 22 | 1 | 0 | 0 | 2 | 5 | 5 | 1 | 2 | 0 | 0 | 2 | 5 |
Unique Cell Count | 223 | 163 | 104 | 124 | 211 | 175 | 291 | 248 | 307 | 224 | 290 | 90 | 156 | 197 | 139 | 92 | 103 | 228 | 168 | 113 | 167 | 191 | 180 | |
Labelled Cell Count | 240 | 184 | 129 | 167 | 258 | 216 | 361 | 291 | 378 | 292 | 370 | 99 | 175 | 243 | 162 | 109 | 111 | 228 | 168 | 113 | 167 | 191 | 180 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 24.8 | 25.0 | 15.2 | 15.6 | 15.4 | 13.2 | 11.7 | 12.3 | – | 12.5 | 11.4 | 11.5 | 23.2 | 22.9 | 23.1 | 24.2 | 24.0 | 24.4 | 23.2 | 25.7 | 25.7 |
Std Deviation (1e-4) | 4.1 | 4.7 | 2.8 | 3.2 | 2.5 | 2.4 | 2.2 | 2.2 | – | 2.9 | 2.7 | 2.2 | 4.3 | 4.3 | 4.2 | 5.2 | 5.1 | 5.4 | 5.2 | 5.7 | 5.6 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.02 | -0.2 | -0.38 | -0.3 | – | -0.28 | -0.42 | -0.4 | 0.61 | 0.59 | 0.61 | 0.67 | 0.66 | 0.68 | 0.61 | 0.76 | 0.76 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.3 | 0.1 | 0 | -0.4 | 0.8 | – | -0.5 | 0.2 | 0 | -3.1 | -2.9 | -2.3 | -0.2 | -0.4 | -1.4 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.8 | 1.1 | 0.9 | 1.3 | 0.5 | – | 1.3 | 2.3 | 2.4 | 1.4 | 1.6 | 3.8 | 5.8 | 5.5 | 5.8 |
Cytoplasm | 0.9 | 0.5 | 1.7 | 0.8 | 2.8 | – | 3.0 | 3.0 | 2.0 | 0.4 | -2.1 | -2.0 | 5.1 | 4.0 | 4.8 |
Endoplasmic Reticulum | -1.2 | -2.1 | -3.1 | -1.6 | -7.4 | – | -4.7 | -8.1 | -6.2 | -0.1 | 1.9 | 1.6 | -12.8 | -11.4 | -11.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 3.4 | 1.8 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 5.1 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.9 | 1.9 | 0 | 3.9 | – | 1.6 | 1.8 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 21.3478 | 25.3865 | 23.9412 | 23.9337 | 20.1972 | 24.1409 | 29.1091 | 29.3205 | 28.3464 | 24.2719 | 25.8915 | 28.8271 | 27.9792 | 27.9827 | 28.0328 | 26.2323 | 25.3836 | 26.837 |
Actin | 0.0189 | 0.0003 | 0.0027 | 0.0057 | 0.0039 | 0.0054 | 0.0082 | 0.0004 | 0.0008 | 0.001 | 0.0005 | 0 | 0.001 | 0 | 0 | 0 | 0 | 0.0003 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0054 | 0.0003 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0004 |
Bud Periphery | 0.0006 | 0.0002 | 0.0011 | 0.0007 | 0.0036 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0012 | 0.0002 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0 |
Bud Site | 0.0013 | 0.005 | 0.0004 | 0.0003 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0104 | 0.0104 | 0.0073 | 0.0084 | 0.0232 | 0.0022 | 0.002 | 0.0076 | 0.0042 | 0.004 | 0.0029 | 0.0009 | 0.0066 | 0.0189 | 0.0035 | 0.0045 | 0.002 | 0.0012 |
Cytoplasm | 0.0075 | 0.008 | 0.0022 | 0.001 | 0.0022 | 0.0056 | 0.0018 | 0.0244 | 0.0024 | 0.0006 | 0.0026 | 0.0005 | 0.0033 | 0.0017 | 0.0006 | 0.0008 | 0.0006 | 0.001 |
Cytoplasmic Foci | 0.0102 | 0.0003 | 0.0003 | 0.0024 | 0.0076 | 0.001 | 0.0024 | 0.0005 | 0.0002 | 0 | 0.0014 | 0 | 0.0008 | 0 | 0 | 0 | 0 | 0 |
Eisosomes | 0.0009 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0016 | 0.0003 | 0 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.821 | 0.935 | 0.9613 | 0.9308 | 0.7759 | 0.9546 | 0.9216 | 0.9385 | 0.975 | 0.9656 | 0.9585 | 0.9849 | 0.9367 | 0.9632 | 0.988 | 0.9804 | 0.9882 | 0.9798 |
Endosome | 0.0153 | 0.0005 | 0.0019 | 0.0082 | 0.027 | 0.0038 | 0.0052 | 0.0013 | 0.0005 | 0.0003 | 0.0068 | 0.0002 | 0.0046 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 |
Golgi | 0.0096 | 0.0002 | 0.0012 | 0.0052 | 0.0113 | 0.0019 | 0.003 | 0.0016 | 0.0005 | 0.0002 | 0.0026 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 |
Lipid Particles | 0.0165 | 0.0081 | 0.0014 | 0.0088 | 0.0185 | 0.0032 | 0.0067 | 0.0069 | 0.0028 | 0.0005 | 0.0066 | 0.0002 | 0.0037 | 0.0041 | 0.0007 | 0.0006 | 0.0003 | 0.0009 |
Mitochondria | 0.0157 | 0.0003 | 0.0045 | 0.0044 | 0.0103 | 0.0012 | 0.0047 | 0.0094 | 0.0045 | 0.0172 | 0.0033 | 0 | 0.0015 | 0.0001 | 0 | 0 | 0 | 0 |
None | 0.0013 | 0.0012 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0013 | 0.0001 | 0.0001 | 0.0001 | 0.0017 | 0 | 0.0013 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0467 | 0.0188 | 0.0088 | 0.0143 | 0.0333 | 0.0129 | 0.0311 | 0.0045 | 0.0052 | 0.0044 | 0.0079 | 0.0108 | 0.031 | 0.0075 | 0.005 | 0.0108 | 0.0068 | 0.0133 |
Nucleolus | 0.0003 | 0.0035 | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0027 | 0.0031 | 0.0005 | 0.0004 | 0.0018 | 0.0005 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0011 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0004 |
Peroxisomes | 0.003 | 0 | 0.0001 | 0.0002 | 0.0491 | 0.0009 | 0.002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0027 | 0.0005 | 0 | 0 | 0.0002 | 0.0002 | 0.0013 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0119 | 0.0037 | 0.0046 | 0.0052 | 0.0191 | 0.005 | 0.0052 | 0.0033 | 0.0027 | 0.0021 | 0.0028 | 0.0017 | 0.0044 | 0.0031 | 0.0019 | 0.0018 | 0.0014 | 0.002 |
Vacuole Periphery | 0.0028 | 0.0006 | 0.0005 | 0.0028 | 0.0064 | 0.001 | 0.0018 | 0.0004 | 0.0002 | 0.0005 | 0.0014 | 0.0003 | 0.0017 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 87.7228 | 70.8604 | 82.0271 | 109.671 | 89.7558 | 84.5053 | 96.0068 | 110.8982 | 98.948 | 116.7069 |
Translational Efficiency | 1.628 | 1.7371 | 1.4538 | 1.1827 | 1.3227 | 1.574 | 1.235 | 1.1028 | 1.2005 | 1.0325 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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