Standard name
Human Ortholog
Description Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.63 0.61 0.89 0.89 0.93 0.94 0.94 0.94 0.91 0.89 0.9 0.9 0.94 0.95 0.97 – – – 0.07 0.1 0.14 0.23 0.21 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0 0 0.05 0 0.09 0.08 0.15 0.1 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.37 0.47 0.17 0.17 0.15 0.11 0.07 0.06 0.07 0.08 0.06 0.06 0 0.05 0 – – – 0.82 0.81 0.75 0.66 0.66 0.67
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 4 0 1 1 1 3
Bud 2 0 0 0 0 1 2 2 6 8 9 5 1 0 0 – – – 4 2 0 6 7 10
Bud Neck 0 0 3 0 0 0 0 0 0 0 0 3 1 2 2 – – – 0 0 3 0 2 3
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 1 1 0 0 0 1 2 1 1 0 1 0 0 – – – 0 0 0 0 0 1
Cytoplasm 410 123 280 237 424 382 433 548 381 447 350 310 299 552 496 – – – 52 22 46 70 86 94
Endoplasmic Reticulum 2 2 4 0 1 0 0 0 1 0 0 1 1 3 2 – – – 6 1 3 1 3 4
Endosome 3 4 2 0 0 1 0 2 0 0 0 0 4 11 5 – – – 29 5 6 6 7 7
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 4 0 2 1 4 3
Mitochondria 96 6 0 2 2 12 25 26 38 42 60 35 0 0 0 – – – 14 4 12 10 15 14
Nucleus 17 4 7 2 2 5 1 5 1 5 3 3 0 2 0 – – – 3 1 0 0 1 0
Nuclear Periphery 1 0 1 0 1 0 1 3 4 3 0 0 0 0 0 – – – 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 1 2 0 0 – – – 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 1 1 2 0 0 0 1 1 0 1 0 2 0 0 1 – – – 2 0 1 1 4 5
Vac/Vac Membrane 242 94 55 45 67 46 33 36 30 38 23 21 14 29 18 – – – 580 176 247 208 278 318
Unique Cell Count 647 201 316 265 456 405 461 585 417 500 389 345 319 583 512 710 218 330 315 421 476
Labelled Cell Count 774 234 357 287 497 447 496 624 464 545 447 381 323 599 524 710 218 330 315 421 476


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 5.0 5.1 4.8 5.2 4.2 3.9 4.5 3.7 3.9 3.5 4.0 5.1 5.3 5.1 – – – 5.2 5.5 5.7
Std Deviation (1e-4) 0.6 1.0 1.2 1.1 1.6 1.4 1.1 1.3 0.9 0.8 0.7 1.3 1.0 1.2 1.0 – – – 1.7 2.0 1.9
Intensity Change (Log2) – – – -0.09 0.01 -0.27 -0.38 -0.18 -0.46 -0.38 -0.56 -0.36 -0.01 0.05 -0.01 – – – 0.03 0.1 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 0.3 2.1 2.8 2.6 2.7 1.2 0.4 0.6 0.5 2.3 3.3 4.8 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 -1.0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -0.1 -1.0 -2.3 -4.4 -5.3 -4.3 -4.3 -4.8 -4.6 -5.3 -6.1 -6.8 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8315 2.0479 1.7359 1.3675 1.697 1.6657 -0.173 1.5214 1.0919 0.3691 0.536 0.9049 -0.1139 1.588 0.9967 0.8941 0.6398 0.7562
Actin 0.0005 0 0.0002 0 0 0.0002 0.0001 0.0001 0.0007 0 0 0 0.0002 0.0001 0.0001 0 0 0
Bud 0.0003 0.0009 0.0019 0.0003 0.0003 0.0007 0.001 0.0014 0.0016 0.0005 0.0016 0.0013 0.0005 0.0017 0.0013 0.0007 0.0007 0.0008
Bud Neck 0.0005 0.0046 0.0013 0.0007 0.0006 0.0032 0.0012 0.0029 0.0036 0.0011 0.0021 0.0033 0.0007 0.0028 0.0019 0.0008 0.0022 0.002
Bud Periphery 0.0005 0.0015 0.0013 0.0004 0.0005 0.0019 0.0031 0.0024 0.0022 0.0007 0.0048 0.0031 0.0007 0.0023 0.0014 0.001 0.0011 0.0014
Bud Site 0.0008 0.0033 0.0035 0.0006 0.001 0.0012 0.0029 0.0062 0.0073 0.002 0.0037 0.0025 0.0011 0.0159 0.0023 0.0022 0.0034 0.0011
Cell Periphery 0.0005 0.001 0.0007 0.0003 0.0003 0.001 0.0011 0.0018 0.0009 0.0004 0.0012 0.0012 0.0005 0.0015 0.0007 0.0004 0.0007 0.0011
Cytoplasm 0.064 0.0673 0.0635 0.0382 0.0238 0.0725 0.0649 0.0687 0.0926 0.0241 0.0293 0.0874 0.0531 0.0969 0.0713 0.0495 0.0244 0.0691
Cytoplasmic Foci 0.0473 0.034 0.0469 0.027 0.0452 0.0356 0.0529 0.0257 0.0526 0.0257 0.0406 0.035 0.0422 0.0259 0.0383 0.0367 0.01 0.028
Eisosomes 0 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0054 0.007 0.005 0.002 0.0018 0.0039 0.0095 0.0096 0.0108 0.0022 0.0045 0.0065 0.0081 0.006 0.0051 0.0021 0.0025 0.005
Endosome 0.3821 0.2287 0.3867 0.3982 0.3853 0.2346 0.2766 0.1698 0.2756 0.3013 0.2338 0.2321 0.3238 0.1734 0.353 0.3614 0.1861 0.2596
Golgi 0.0051 0.0028 0.0062 0.0027 0.0039 0.0024 0.0038 0.002 0.0052 0.0029 0.0036 0.0027 0.0036 0.0023 0.0052 0.0041 0.001 0.0028
Lipid Particles 0.0126 0.0057 0.0084 0.0012 0.0058 0.0064 0.0131 0.0068 0.0136 0.0089 0.0076 0.0083 0.0181 0.0047 0.0039 0.0024 0.0023 0.0043
Mitochondria 0.0099 0.0034 0.0076 0.002 0.0025 0.0059 0.0113 0.003 0.0265 0.0018 0.0031 0.0061 0.0051 0.005 0.0116 0.0036 0.0006 0.0042
None 0.0871 0.088 0.0529 0.033 0.0095 0.0512 0.1075 0.0677 0.1141 0.0268 0.0378 0.0344 0.0465 0.0557 0.0248 0.0138 0.0056 0.0214
Nuclear Periphery 0.0058 0.008 0.0072 0.002 0.0035 0.0068 0.007 0.0111 0.0107 0.0034 0.0055 0.0074 0.0089 0.0118 0.0111 0.0029 0.0032 0.009
Nucleolus 0.0023 0.0048 0.0036 0.001 0.0015 0.0051 0.0048 0.0072 0.0076 0.0041 0.0049 0.0054 0.0025 0.0041 0.0032 0.0018 0.0069 0.0025
Nucleus 0.0042 0.0152 0.0089 0.0027 0.0044 0.0218 0.0108 0.0184 0.0152 0.0109 0.0162 0.0231 0.0087 0.0126 0.0093 0.0039 0.0116 0.0184
Peroxisomes 0.0017 0.0011 0.0016 0.0005 0.0013 0.0011 0.0017 0.0011 0.0023 0.0009 0.0031 0.0012 0.0013 0.0007 0.0008 0.0006 0.0004 0.0007
Punctate Nuclear 0.0031 0.0039 0.0011 0.0003 0.0009 0.0016 0.0035 0.0015 0.0083 0.0017 0.0075 0.0024 0.0027 0.0013 0.0014 0.0003 0.0005 0.0016
Vacuole 0.3184 0.4711 0.3408 0.4318 0.442 0.487 0.3743 0.5459 0.3106 0.5322 0.5359 0.485 0.4235 0.5264 0.4006 0.4561 0.6821 0.5093
Vacuole Periphery 0.0478 0.0475 0.0506 0.0547 0.0658 0.056 0.0486 0.0468 0.0378 0.0483 0.0534 0.0517 0.0478 0.0491 0.0527 0.0556 0.0547 0.0575

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.9998 7.8491 2.5886 9.2908 7.5895 16.576 9.6999 8.6298 6.8347 15.5603
Translational Efficiency 0.7245 0.9768 1.4953 0.7852 1.5022 0.9843 0.6895 0.6015 1.1224 0.732

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (82%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Csh1

Csh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Csh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available