Standard name | |
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Human Ortholog | |
Description | Prephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 6 | 6 | 9 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 2 | 8 | 2 | 5 | 5 | 9 | 3 | 6 | 8 | 8 | 10 | 1 | 5 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 380 | 264 | 381 | 198 | 266 | 248 | 302 | 280 | 332 | 405 | 287 | 292 | 445 | 502 | 477 | 79 | 122 | 114 | 361 | 249 | 359 | 303 | 401 | 416 |
Endoplasmic Reticulum | 1 | 0 | 6 | 1 | 3 | 1 | 14 | 9 | 19 | 18 | 3 | 12 | 2 | 6 | 3 | 3 | 5 | 1 | 1 | 1 | 6 | 1 | 0 | 6 |
Endosome | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 1 | 1 | 0 |
Golgi | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 3 | 1 | 0 | 2 |
Mitochondria | 3 | 2 | 0 | 3 | 6 | 36 | 82 | 58 | 79 | 134 | 138 | 126 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 1 | 1 |
Nucleus | 0 | 1 | 1 | 1 | 1 | 2 | 5 | 4 | 9 | 11 | 4 | 3 | 3 | 2 | 4 | 1 | 3 | 2 | 0 | 4 | 0 | 6 | 5 | 6 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 0 | 1 | 3 | 4 | 7 | 14 | 25 | 35 | 54 | 53 | 63 | 60 | 2 | 20 | 2 | 5 | 6 | 2 | 0 | 1 | 3 | 4 | 7 | 5 |
Unique Cell Count | 385 | 267 | 391 | 205 | 280 | 281 | 391 | 365 | 460 | 566 | 434 | 446 | 448 | 517 | 479 | 83 | 128 | 117 | 375 | 264 | 390 | 326 | 421 | 449 |
Labelled Cell Count | 390 | 270 | 402 | 212 | 288 | 306 | 441 | 396 | 505 | 638 | 515 | 508 | 454 | 535 | 488 | 88 | 140 | 120 | 375 | 264 | 390 | 326 | 421 | 449 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.5 | 13.8 | 11.8 | 10.6 | 10.2 | 8.6 | 7.9 | 8.0 | 7.1 | 7.2 | 6.5 | 6.6 | 13.4 | 12.8 | 12.4 | 14.8 | 12.8 | 12.7 | 13.0 | 13.9 | 15.2 |
Std Deviation (1e-4) | 1.8 | 2.1 | 1.8 | 2.2 | 1.7 | 1.6 | 1.7 | 1.8 | 1.4 | 1.5 | 1.5 | 1.5 | 2.1 | 1.9 | 1.9 | 2.6 | 2.9 | 2.4 | 2.2 | 2.4 | 2.4 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.21 | -0.46 | -0.59 | -0.57 | -0.73 | -0.71 | -0.85 | -0.84 | 0.18 | 0.11 | 0.07 | 0.32 | 0.11 | 0.1 | 0.14 | 0.24 | 0.37 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.2 | -0.2 | 0.2 | -1.4 | -0.8 | -0.8 | -0.2 | 0.2 | 0 | -1.4 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -1.7 | -4.8 | -8.5 | -8.6 | -10.0 | -10.3 | -11.4 | -11.6 | 2.2 | -0.3 | 2.7 | -1.1 | -1.2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1.8 | 0.9 | 2.2 | 1.6 | 0 | 1.2 | 0 | -0.5 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 7.3 | 9.6 | 8.2 | 8.6 | 10.4 | 12.2 | 11.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.4 | 4.2 | 5.5 | 6.4 | 5.6 | 7.3 | 6.9 | 0 | 2.9 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.0163 | 13.0999 | 12.1465 | 11.3701 | 10.9265 | 11.8077 | 11.7894 | 13.7753 | 13.1945 | 11.7594 | 12.1779 | 12.2688 | 9.1378 | 11.6034 | 9.7404 | 9.4552 | 9.4725 | 10.0979 |
Actin | 0.002 | 0.0004 | 0.0023 | 0.0011 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0159 | 0.0002 | 0.0024 | 0.0516 | 0.0397 | 0.0033 |
Bud | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0032 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0009 | 0.0001 |
Bud Neck | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.001 | 0.0003 | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0009 | 0.0008 | 0.0001 | 0.0005 | 0.0014 | 0.0004 | 0.0007 |
Bud Periphery | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0013 | 0 |
Bud Site | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.001 | 0 | 0.0001 | 0 | 0.0002 | 0.0014 | 0.0001 | 0.0003 | 0.0004 | 0.0026 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.9582 | 0.9738 | 0.9594 | 0.9454 | 0.9644 | 0.9625 | 0.9407 | 0.953 | 0.9341 | 0.9438 | 0.92 | 0.9309 | 0.9019 | 0.9905 | 0.9471 | 0.8933 | 0.904 | 0.9545 |
Cytoplasmic Foci | 0.0027 | 0.0012 | 0.0023 | 0.0028 | 0.0013 | 0.0028 | 0.0015 | 0.0021 | 0.001 | 0.003 | 0.0011 | 0.0015 | 0.0121 | 0.0008 | 0.0025 | 0.0088 | 0.0026 | 0.003 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0003 | 0.0006 | 0 |
Endoplasmic Reticulum | 0.0063 | 0.0009 | 0.0015 | 0.0019 | 0.0013 | 0.0036 | 0.0031 | 0.0016 | 0.0016 | 0.0021 | 0.0017 | 0.0021 | 0.0053 | 0.0006 | 0.0029 | 0.0078 | 0.0013 | 0.0016 |
Endosome | 0.0018 | 0.0006 | 0.0016 | 0.0011 | 0.0006 | 0.0022 | 0.0012 | 0.0014 | 0.0008 | 0.0005 | 0.0007 | 0.0011 | 0.0097 | 0.0004 | 0.0064 | 0.0098 | 0.0034 | 0.0027 |
Golgi | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0013 | 0.0001 | 0.0012 | 0.0016 | 0.0027 | 0.0013 |
Lipid Particles | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0019 | 0 | 0.0032 | 0.0019 | 0.0032 | 0.002 |
Mitochondria | 0.0002 | 0.0001 | 0.0029 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.003 | 0.0001 | 0.0014 | 0.0003 | 0.0163 | 0.0002 |
None | 0.0042 | 0.0009 | 0.0017 | 0.0112 | 0.0012 | 0.0015 | 0.0022 | 0.0015 | 0.0012 | 0.0027 | 0.0012 | 0.0013 | 0.0069 | 0.0008 | 0.0078 | 0.0042 | 0.0022 | 0.0019 |
Nuclear Periphery | 0.0038 | 0.0012 | 0.0019 | 0.0024 | 0.0025 | 0.0025 | 0.0043 | 0.0023 | 0.0031 | 0.0021 | 0.0027 | 0.003 | 0.009 | 0.0009 | 0.0053 | 0.0038 | 0.0022 | 0.0026 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0016 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0178 | 0.0195 | 0.023 | 0.0316 | 0.0265 | 0.0213 | 0.0443 | 0.0291 | 0.0564 | 0.0265 | 0.0704 | 0.0564 | 0.022 | 0.0049 | 0.0138 | 0.0107 | 0.0106 | 0.0224 |
Peroxisomes | 0.0001 | 0 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0011 | 0 | 0.0015 | 0.0005 | 0.0036 | 0.0014 |
Punctate Nuclear | 0.0008 | 0.0003 | 0.0004 | 0.0005 | 0.0005 | 0.0004 | 0.001 | 0.0007 | 0.0007 | 0.0169 | 0.0006 | 0.0008 | 0.0035 | 0.0002 | 0.0011 | 0.0018 | 0.0006 | 0.002 |
Vacuole | 0.0006 | 0.0002 | 0.0005 | 0.0004 | 0.0005 | 0.0008 | 0.0007 | 0.0007 | 0.0004 | 0.001 | 0.0007 | 0.0007 | 0.0013 | 0.0002 | 0.0017 | 0.001 | 0.0011 | 0.0003 |
Vacuole Periphery | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0 | 0.0005 | 0.0002 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.0114 | 23.9517 | 19.389 | 29.7612 | 29.4755 | 25.1557 | 20.9152 | 26.9944 | 21.4595 | 30.7773 |
Translational Efficiency | 1.4515 | 1.5749 | 1.738 | 1.0465 | 1.1317 | 1.4489 | 1.8869 | 0.9596 | 1.4013 | 1.0323 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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