Standard name
Human Ortholog
Description Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop6p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.07 0.06 0.1 0.12 0.13 0.11 0.1 0.1 0.12 0.14 0.16 0.21 0.34 0.34 0.21 0 0 0 0 0 0.07
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.12 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.2 0.63 0.6 0.24 0.55 0.61 0.67 0.68 0.69 0.68 0.05 0 0 0.07 0.1 0.1 0 0 0 0 0 0
Nucleus 0.85 0.84 0.66 0.74 0.72 0.65 0.59 0.59 0.62 0.58 0.55 0.79 0.76 0.7 0.52 0.49 0.57 0.77 0.71 0.61 0.59 0.53 0.36
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.12 0.05 0 0.05 0 0 0 0 0 0.05 0 0.07 0.07 0.2 0.15 0.21 0.08 0.14 0.17 0.18 0.27 0.35
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 7 3 6 5 4 7 16 13 7 0 1 0 1 1 0 10 1 3 1 4 4
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 2 0 1 0 1 1
Bud Site 0 1 0 4 4 5 14 12 25 12 12 0 0 0 0 0 0
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 30 1 29 25 55 40 52 37 48 31 43 33 57 78 19 23 9 13 0 18 6 10 23
Endoplasmic Reticulum 2 0 3 0 0 0 1 0 0 0 0 0 2 0 4 8 3 0 0 1 0 1 0
Endosome 4 0 0 0 22 1 1 0 2 0 1 4 3 5 2 1 1 9 1 4 2 5 13
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0
Mitochondria 77 5 260 234 136 178 246 226 322 221 235 11 6 4 4 7 4 4 0 5 2 6 9
Nucleus 421 21 273 288 404 210 237 201 294 186 190 189 263 258 29 33 24 426 51 264 90 164 122
Nuclear Periphery 2 0 2 12 3 3 7 4 7 3 1 2 3 4 0 0 0 1 0 6 0 1 3
Nucleolus 1 0 1 2 5 7 3 11 7 7 6 4 5 3 0 1 0 21 1 19 11 11 22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 1 3 0 2 1 3 3 1 2 2 1 1 2 1 13 1 18 6 8 10
Vac/Vac Membrane 26 3 22 14 31 13 8 12 13 14 16 9 24 24 11 10 9 46 10 75 26 84 118
Unique Cell Count 496 25 413 389 565 325 400 339 474 322 345 239 346 366 56 67 42 556 72 431 153 307 343
Labelled Cell Count 567 31 599 583 670 462 575 511 737 490 512 254 368 378 71 87 51 556 72 431 153 307 343


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.3 3.5 3.6 4.5 3.7 3.9 3.5 3.6 3.6 3.7 5.6 5.4 5.5 6.4 5.5 5.8 5.6 5.4 5.6
Std Deviation (1e-4) 0.7 1.8 0.8 1.4 1.5 1.6 1.7 2.0 1.4 2.0 2.0 2.6 1.3 1.9 2.0 2.0 1.6 2.8 1.6 2.2
Intensity Change (Log2) 0.07 0.37 0.11 0.18 0.01 0.07 0.05 0.09 0.7 0.64 0.67 0.88 0.66 0.75 0.7 0.66 0.7


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 1.5 2.4 2.8 1.9 1.6 1.3 2.5 2.8 4.1 5.8 6.2 6.7 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -14.6 -17.6 -18.2 -7.9 -8.0 -6.7
Nucleus 2.4 1.8 -0.4 -2.0 -1.9 -1.3 -2.3 -3.1 3.5 3.0 1.3 -2.1 -2.7 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.2 0.1 -0.8 -2.5 -1.2 -2.0 -0.6 -0.4 -0.9 0.9 0.7 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8608 1.7769 1.261 1.2888 1.2277 1.4841 1.7027 0.9534 1.0124 0.7733 0.9571 0.8535 2.9857 2.6799 2.3966 2.4285 2.7133 2.332
Actin 0.0418 0.0033 0.0047 0.001 0.0001 0.0131 0.0118 0 0.0152 0.0483 0.0205 0.0124 0.0352 0.0001 0.011 0.0012 0.0084 0.006
Bud 0.0012 0.0002 0.0003 0.0003 0 0.0002 0.0006 0.0001 0.001 0.0012 0.0002 0.0003 0.0006 0.0002 0.0006 0.0005 0.0001 0.0001
Bud Neck 0.0069 0.0007 0.0005 0.0005 0.0002 0.0018 0.0065 0.0005 0.0092 0.0004 0.0008 0.0018 0.0118 0.0008 0.0021 0.0008 0.0005 0.0017
Bud Periphery 0.0033 0.0003 0.0007 0.0007 0.0001 0.0006 0.0024 0.0001 0.0014 0.0013 0.0004 0.0015 0.0016 0.0003 0.0009 0.0006 0.0003 0.0001
Bud Site 0.0265 0.0012 0.0011 0.0011 0.0001 0.0005 0.0057 0.0004 0.0138 0.0102 0.0003 0.0006 0.002 0.0029 0.0032 0.0024 0.0002 0.0001
Cell Periphery 0.0102 0.0002 0.0002 0.0001 0 0.0001 0.0042 0.0001 0.0007 0.0001 0.0001 0.0006 0.0003 0.0001 0.0003 0.0001 0 0.0001
Cytoplasm 0.0034 0.0021 0.0057 0.0005 0.0003 0.0116 0.0095 0.0025 0.0067 0.0602 0.0004 0.0009 0.0073 0.001 0.0032 0.0024 0.0024 0.0006
Cytoplasmic Foci 0.0189 0.0012 0.0065 0.0088 0 0.0023 0.029 0.0001 0.0048 0.0037 0.0218 0.0006 0.004 0.0002 0.0007 0.0031 0.0037 0.0003
Eisosomes 0.0007 0.0001 0 0 0 0 0.0004 0 0.0028 0.0001 0.0003 0.0005 0.0007 0 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0031 0.0017 0.0015 0.0002 0 0.0003 0.011 0.0006 0.0023 0.0007 0.0007 0.0064 0.0039 0.0001 0.0023 0.0011 0.0006 0.0004
Endosome 0.0356 0.005 0.01 0.0097 0.0001 0.0048 0.0438 0.0005 0.0075 0.0026 0.0064 0.007 0.0169 0.0003 0.004 0.0203 0.0123 0.001
Golgi 0.0121 0.0026 0.0027 0.0048 0 0.0062 0.0097 0 0.0043 0.002 0.0117 0.0027 0.0048 0.0001 0.0016 0.0019 0.0049 0.0005
Lipid Particles 0.0382 0.0013 0.0058 0.047 0 0.0024 0.0212 0.0001 0.0092 0.0009 0.0074 0.0014 0.0066 0.0001 0.0065 0.0021 0.0261 0.0003
Mitochondria 0.0579 0.0038 0.0027 0.0029 0.0009 0.0089 0.012 0.0003 0.0059 0.0012 0.0329 0.0152 0.0175 0.0003 0.0018 0.002 0.0014 0.0004
None 0.0034 0.0011 0.0048 0.0003 0.0001 0.0005 0.0153 0.0019 0.0194 0.0063 0.0004 0.0003 0.0136 0.0052 0.001 0.0043 0.0029 0.0089
Nuclear Periphery 0.0198 0.0202 0.0096 0.0023 0.0067 0.0124 0.0616 0.0158 0.0063 0.0067 0.0014 0.0069 0.0347 0.0047 0.0157 0.0092 0.0168 0.0109
Nucleolus 0.0535 0.0897 0.0544 0.0148 0.0293 0.0684 0.037 0.0669 0.0885 0.0175 0.0438 0.0739 0.0445 0.0576 0.0822 0.0946 0.0436 0.0796
Nucleus 0.5974 0.8564 0.8726 0.8785 0.9568 0.8593 0.6663 0.8972 0.7803 0.8318 0.8202 0.8449 0.7633 0.9226 0.8557 0.8397 0.8363 0.8828
Peroxisomes 0.0128 0.0002 0.0042 0.0209 0 0.0026 0.0049 0 0.0054 0.0032 0.0276 0.0001 0.004 0.0001 0.0004 0.0011 0.0083 0.0001
Punctate Nuclear 0.0275 0.0062 0.0064 0.0043 0.0048 0.0018 0.0265 0.0109 0.0047 0.0011 0.0019 0.0159 0.0203 0.0028 0.004 0.0078 0.0303 0.0053
Vacuole 0.0168 0.0013 0.0044 0.001 0.0002 0.0013 0.0137 0.0014 0.0088 0.0004 0.0003 0.0044 0.0038 0.0006 0.0018 0.0038 0.0003 0.0004
Vacuole Periphery 0.0087 0.0012 0.0015 0.0004 0.0003 0.0007 0.0069 0.0006 0.0018 0.0003 0.0005 0.0016 0.0024 0.0001 0.001 0.0009 0.0005 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.1298 9.3274 12.1124 14.4127 5.0731 7.8212 10.0204 14.3218 14.1211 15.6711
Translational Efficiency 3.6007 2.5378 1.821 1.3655 3.6241 3.6719 2.8239 1.3933 1.5109 1.6627

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop6p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA
Localization
Cell Percentages nucleus (68%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Pop7

Pop7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pop7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available