Standard name | |
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Human Ortholog | |
Description | Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 15 | 24 | 13 | 4 | 4 | 2 | 6 | 3 | 4 | 1 | 5 | 2 | 48 | 14 | 27 | 17 | 9 | 12 | 38 | 57 | 45 | 23 | 51 | 65 |
Bud | 45 | 26 | 30 | 35 | 66 | 62 | 96 | 78 | 60 | 66 | 52 | 51 | 17 | 9 | 19 | 27 | 15 | 13 | 1 | 2 | 6 | 6 | 6 | 6 |
Bud Neck | 10 | 1 | 8 | 6 | 7 | 1 | 6 | 3 | 2 | 3 | 0 | 1 | 17 | 27 | 24 | 7 | 9 | 3 | 10 | 17 | 10 | 10 | 15 | 17 |
Bud Site | 18 | 7 | 13 | 13 | 19 | 41 | 43 | 24 | 18 | 24 | 23 | 31 | 7 | 0 | 3 | 8 | 4 | 3 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Cytoplasm | 33 | 44 | 58 | 37 | 31 | 40 | 53 | 32 | 36 | 50 | 22 | 21 | 39 | 96 | 114 | 59 | 57 | 28 | 3 | 4 | 5 | 9 | 10 | 14 |
Endoplasmic Reticulum | 4 | 16 | 3 | 1 | 1 | 1 | 0 | 3 | 5 | 0 | 9 | 3 | 8 | 1 | 7 | 10 | 9 | 8 | 0 | 1 | 3 | 0 | 0 | 1 |
Endosome | 10 | 26 | 22 | 19 | 25 | 12 | 3 | 17 | 7 | 3 | 4 | 2 | 1 | 13 | 13 | 32 | 25 | 10 | 58 | 52 | 46 | 25 | 21 | 33 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 2 | 4 | 0 | 1 | 0 |
Mitochondria | 128 | 77 | 31 | 35 | 61 | 195 | 279 | 201 | 259 | 320 | 348 | 311 | 4 | 0 | 1 | 30 | 11 | 4 | 43 | 38 | 43 | 20 | 31 | 31 |
Nucleus | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 3 | 2 | 4 | 1 | 0 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 3 | 0 | 0 | 1 | 4 | 4 | 0 | 5 | 6 | 5 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Peroxisomes | 43 | 52 | 72 | 89 | 81 | 120 | 114 | 92 | 40 | 47 | 42 | 52 | 6 | 8 | 16 | 29 | 23 | 17 | 126 | 78 | 156 | 52 | 71 | 77 |
SpindlePole | 67 | 28 | 164 | 78 | 101 | 100 | 148 | 141 | 58 | 78 | 31 | 55 | 80 | 148 | 129 | 114 | 78 | 37 | 28 | 19 | 29 | 18 | 20 | 16 |
Vac/Vac Membrane | 1 | 4 | 1 | 0 | 2 | 2 | 0 | 5 | 4 | 3 | 6 | 9 | 0 | 1 | 1 | 4 | 3 | 2 | 1 | 7 | 1 | 1 | 4 | 3 |
Unique Cell Count | 264 | 227 | 309 | 209 | 259 | 377 | 502 | 402 | 360 | 440 | 406 | 399 | 168 | 275 | 289 | 244 | 183 | 105 | 322 | 286 | 360 | 173 | 244 | 276 |
Labelled Cell Count | 378 | 307 | 418 | 317 | 399 | 579 | 754 | 605 | 493 | 604 | 556 | 550 | 230 | 318 | 359 | 341 | 248 | 140 | 322 | 286 | 360 | 173 | 244 | 276 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.5 | 4.5 | 4.3 | 4.2 | 3.5 | 3.4 | 3.5 | 3.3 | 3.3 | 3.0 | 3.2 | 5.6 | 5.9 | 5.5 | 5.7 | 5.7 | 5.7 | 5.3 | 5.9 | 6.1 |
Std Deviation (1e-4) | 0.5 | 0.7 | 1.8 | 2.4 | 1.7 | 1.8 | 1.3 | 1.3 | 0.5 | 1.0 | 0.6 | 0.8 | 0.8 | 1.3 | 1.3 | 1.5 | 1.9 | 2.0 | 1.2 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.1 | -0.36 | -0.41 | -0.34 | -0.46 | -0.46 | -0.6 | -0.5 | 0.32 | 0.38 | 0.28 | 0.34 | 0.35 | 0.34 | 0.24 | 0.39 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3162 | 0.5623 | 0.1437 | -0.4383 | -0.3132 | 0.0709 | 0.2023 | 0.0258 | 0.0306 | -0.0471 | -0.0559 | 0.2689 | -0.8097 | -0.3669 | -0.6695 | -0.6241 | -0.8079 | -0.6209 |
Actin | 0.0129 | 0.0053 | 0.0128 | 0.032 | 0.0186 | 0.008 | 0.0859 | 0.003 | 0.0344 | 0.0084 | 0.0073 | 0.0105 | 0.0213 | 0.006 | 0.0287 | 0.0146 | 0.0004 | 0.0032 |
Bud | 0.0001 | 0.0034 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0057 | 0.0035 | 0.0026 | 0.0027 | 0.0001 | 0.0023 |
Bud Neck | 0.0006 | 0.0008 | 0.0043 | 0.0026 | 0.0007 | 0.0066 | 0.0011 | 0.0024 | 0.0055 | 0.0009 | 0.0008 | 0.01 | 0.0015 | 0.0031 | 0.0023 | 0.001 | 0.0048 | 0.0013 |
Bud Periphery | 0.0002 | 0.0011 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0014 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0006 | 0.0048 | 0.0011 | 0.0021 | 0.0047 | 0.0003 | 0.0017 |
Bud Site | 0.0021 | 0.0147 | 0.0012 | 0.0011 | 0.0007 | 0.0002 | 0.0057 | 0.0171 | 0.0071 | 0.0002 | 0.0009 | 0.001 | 0.0049 | 0.0148 | 0.0088 | 0.0041 | 0.0002 | 0.0068 |
Cell Periphery | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0013 | 0 | 0.0002 |
Cytoplasm | 0.0058 | 0.0072 | 0.0078 | 0.0025 | 0.0024 | 0.0084 | 0.0157 | 0.0346 | 0.018 | 0.0306 | 0.0097 | 0.0173 | 0.0099 | 0.0249 | 0.0154 | 0.0206 | 0.0029 | 0.0049 |
Cytoplasmic Foci | 0.2216 | 0.2175 | 0.2481 | 0.2784 | 0.2329 | 0.2408 | 0.2567 | 0.29 | 0.3091 | 0.243 | 0.3425 | 0.271 | 0.2739 | 0.2956 | 0.3007 | 0.2898 | 0.303 | 0.3137 |
Eisosomes | 0.0006 | 0.0002 | 0.0007 | 0.0009 | 0.0005 | 0.0006 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0008 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0014 | 0.0012 | 0.0013 | 0.0003 | 0.0004 | 0.0005 | 0.0048 | 0.003 | 0.0032 | 0.002 | 0.0013 | 0.0025 | 0.003 | 0.0008 | 0.0012 | 0.0023 | 0.0002 | 0.0005 |
Endosome | 0.0596 | 0.0998 | 0.0526 | 0.1055 | 0.0328 | 0.0339 | 0.0712 | 0.1223 | 0.09 | 0.0741 | 0.1035 | 0.0666 | 0.0733 | 0.1042 | 0.0573 | 0.034 | 0.1234 | 0.0382 |
Golgi | 0.0168 | 0.024 | 0.0166 | 0.0176 | 0.0159 | 0.0125 | 0.0233 | 0.0249 | 0.0222 | 0.0386 | 0.0223 | 0.0276 | 0.0214 | 0.0197 | 0.0192 | 0.0147 | 0.0192 | 0.0138 |
Lipid Particles | 0.3278 | 0.2512 | 0.2028 | 0.1836 | 0.2609 | 0.2651 | 0.2237 | 0.2132 | 0.1411 | 0.2124 | 0.1334 | 0.2204 | 0.3049 | 0.2605 | 0.2594 | 0.1698 | 0.2644 | 0.2956 |
Mitochondria | 0.004 | 0.0145 | 0.0112 | 0.0094 | 0.0187 | 0.0102 | 0.022 | 0.0028 | 0.0115 | 0.0244 | 0.0033 | 0.0283 | 0.0037 | 0.0061 | 0.0085 | 0.0109 | 0.0053 | 0.0124 |
None | 0.0898 | 0.0346 | 0.1318 | 0.0762 | 0.1114 | 0.05 | 0.0655 | 0.1048 | 0.0702 | 0.0801 | 0.0046 | 0.0582 | 0.0519 | 0.0249 | 0.0531 | 0.1327 | 0.045 | 0.0495 |
Nuclear Periphery | 0.0032 | 0.0014 | 0.0021 | 0.0003 | 0.0012 | 0.0003 | 0.008 | 0.0025 | 0.0019 | 0.0004 | 0.0043 | 0.0012 | 0.0025 | 0.001 | 0.0026 | 0.0022 | 0.0006 | 0.0012 |
Nucleolus | 0.0019 | 0.0012 | 0.0011 | 0.001 | 0.0003 | 0.0003 | 0.0009 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0006 | 0.0014 | 0.0013 | 0.0012 | 0.0013 | 0.0004 | 0.0011 |
Nucleus | 0.0014 | 0.0007 | 0.0037 | 0.0003 | 0.0002 | 0.0002 | 0.0012 | 0.0008 | 0.0007 | 0.0002 | 0.0005 | 0.0005 | 0.001 | 0.0009 | 0.0012 | 0.0013 | 0.0002 | 0.0011 |
Peroxisomes | 0.2336 | 0.303 | 0.288 | 0.2789 | 0.2883 | 0.3497 | 0.1764 | 0.1558 | 0.2668 | 0.2771 | 0.3466 | 0.2438 | 0.2047 | 0.2077 | 0.2151 | 0.2809 | 0.1835 | 0.2271 |
Punctate Nuclear | 0.0141 | 0.0097 | 0.0116 | 0.0032 | 0.0125 | 0.0105 | 0.0312 | 0.0179 | 0.0134 | 0.0025 | 0.0147 | 0.0364 | 0.0075 | 0.0193 | 0.0188 | 0.0079 | 0.0448 | 0.0225 |
Vacuole | 0.002 | 0.0069 | 0.0012 | 0.0055 | 0.0005 | 0.0015 | 0.002 | 0.0032 | 0.002 | 0.0025 | 0.0027 | 0.0018 | 0.0016 | 0.0039 | 0.001 | 0.0018 | 0.0009 | 0.0018 |
Vacuole Periphery | 0.0004 | 0.0013 | 0.0006 | 0.0008 | 0.0005 | 0.0003 | 0.0014 | 0.0006 | 0.0005 | 0.0022 | 0.001 | 0.0009 | 0.0005 | 0.0007 | 0.0004 | 0.0006 | 0.0003 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.7725 | 3.9709 | 4.7145 | 9.4183 | 3.4556 | 6.2154 | 6.6247 | 5.9617 | 8.0628 | 10.6745 |
Translational Efficiency | 0.332 | 0.7159 | 0.7079 | 0.4021 | 0.7749 | 0.4994 | 0.3369 | 0.5703 | 0.4196 | 0.3557 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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