Standard name
Human Ortholog
Description Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0.29 0.05 0.09 0.07 0.05 0.11 0.12 0.2 0.13 0.13 0.21 0.24
Bud 0.17 0.11 0.1 0.17 0.25 0.16 0.19 0.19 0.17 0.15 0.13 0.13 0.1 0 0.07 0.11 0.08 0.12 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.08 0 0.05 0 0 0.06 0 0.06 0.06 0.06
Bud Site 0.07 0 0 0.06 0.07 0.11 0.09 0.06 0.05 0.05 0.06 0.08 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.19 0.19 0.18 0.12 0.11 0.11 0.08 0.1 0.11 0.05 0.05 0.23 0.35 0.39 0.24 0.31 0.27 0 0 0 0.05 0 0.05
Endoplasmic Reticulum 0 0.07 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.08 0 0 0 0 0 0
Endosome 0 0.11 0.07 0.09 0.1 0 0 0 0 0 0 0 0 0.05 0 0.13 0.14 0.1 0.18 0.18 0.13 0.15 0.09 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.48 0.34 0.1 0.17 0.24 0.52 0.56 0.5 0.72 0.73 0.86 0.78 0 0 0 0.12 0.06 0 0.14 0.13 0.12 0.12 0.13 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.16 0.23 0.23 0.43 0.31 0.32 0.23 0.23 0.11 0.11 0.1 0.13 0 0 0.06 0.12 0.13 0.16 0.39 0.28 0.43 0.3 0.29 0.28
SpindlePole 0.25 0.12 0.53 0.37 0.39 0.27 0.29 0.35 0.16 0.18 0.08 0.14 0.48 0.54 0.45 0.47 0.43 0.35 0.09 0.07 0.08 0.11 0.08 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 15 24 13 4 4 2 6 3 4 1 5 2 48 14 27 17 9 12 38 57 45 23 51 65
Bud 45 26 30 35 66 62 96 78 60 66 52 51 17 9 19 27 15 13 1 2 6 6 6 6
Bud Neck 10 1 8 6 7 1 6 3 2 3 0 1 17 27 24 7 9 3 10 17 10 10 15 17
Bud Site 18 7 13 13 19 41 43 24 18 24 23 31 7 0 3 8 4 3
Cell Periphery 2 2 0 0 0 0 1 0 0 1 3 2 2 1 4 1 0 0 0 0 0 0 2 0
Cytoplasm 33 44 58 37 31 40 53 32 36 50 22 21 39 96 114 59 57 28 3 4 5 9 10 14
Endoplasmic Reticulum 4 16 3 1 1 1 0 3 5 0 9 3 8 1 7 10 9 8 0 1 3 0 0 1
Endosome 10 26 22 19 25 12 3 17 7 3 4 2 1 13 13 32 25 10 58 52 46 25 21 33
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 3 2 4 0 1 0
Mitochondria 128 77 31 35 61 195 279 201 259 320 348 311 4 0 1 30 11 4 43 38 43 20 31 31
Nucleus 2 0 0 0 1 1 1 1 0 3 2 4 1 0 1 2 3 1 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 1 0 1 0 0 3 1 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 3 0 0 1 4 4 0 5 6 5 0 0 0 1 1 0 0 1 0 0 0 0
Peroxisomes 43 52 72 89 81 120 114 92 40 47 42 52 6 8 16 29 23 17 126 78 156 52 71 77
SpindlePole 67 28 164 78 101 100 148 141 58 78 31 55 80 148 129 114 78 37 28 19 29 18 20 16
Vac/Vac Membrane 1 4 1 0 2 2 0 5 4 3 6 9 0 1 1 4 3 2 1 7 1 1 4 3
Unique Cell Count 264 227 309 209 259 377 502 402 360 440 406 399 168 275 289 244 183 105 322 286 360 173 244 276
Labelled Cell Count 378 307 418 317 399 579 754 605 493 604 556 550 230 318 359 341 248 140 322 286 360 173 244 276


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.5 4.5 4.3 4.2 3.5 3.4 3.5 3.3 3.3 3.0 3.2 5.6 5.9 5.5 5.7 5.7 5.7 5.3 5.9 6.1
Std Deviation (1e-4) 0.5 0.7 1.8 2.4 1.7 1.8 1.3 1.3 0.5 1.0 0.6 0.8 0.8 1.3 1.3 1.5 1.9 2.0 1.2 1.5 1.6
Intensity Change (Log2) -0.07 -0.1 -0.36 -0.41 -0.34 -0.46 -0.46 -0.6 -0.5 0.32 0.38 0.28 0.34 0.35 0.34 0.24 0.39 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3162 0.5623 0.1437 -0.4383 -0.3132 0.0709 0.2023 0.0258 0.0306 -0.0471 -0.0559 0.2689 -0.8097 -0.3669 -0.6695 -0.6241 -0.8079 -0.6209
Actin 0.0129 0.0053 0.0128 0.032 0.0186 0.008 0.0859 0.003 0.0344 0.0084 0.0073 0.0105 0.0213 0.006 0.0287 0.0146 0.0004 0.0032
Bud 0.0001 0.0034 0.0001 0.0001 0.0003 0.0001 0.0008 0.0001 0.0005 0.0001 0.0001 0.0003 0.0057 0.0035 0.0026 0.0027 0.0001 0.0023
Bud Neck 0.0006 0.0008 0.0043 0.0026 0.0007 0.0066 0.0011 0.0024 0.0055 0.0009 0.0008 0.01 0.0015 0.0031 0.0023 0.001 0.0048 0.0013
Bud Periphery 0.0002 0.0011 0.0002 0.0001 0.0006 0.0002 0.0014 0.0001 0.0008 0.0001 0.0001 0.0006 0.0048 0.0011 0.0021 0.0047 0.0003 0.0017
Bud Site 0.0021 0.0147 0.0012 0.0011 0.0007 0.0002 0.0057 0.0171 0.0071 0.0002 0.0009 0.001 0.0049 0.0148 0.0088 0.0041 0.0002 0.0068
Cell Periphery 0.0002 0.0004 0.0002 0.0001 0.0001 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0.0002 0.0003 0.0002 0.0002 0.0013 0 0.0002
Cytoplasm 0.0058 0.0072 0.0078 0.0025 0.0024 0.0084 0.0157 0.0346 0.018 0.0306 0.0097 0.0173 0.0099 0.0249 0.0154 0.0206 0.0029 0.0049
Cytoplasmic Foci 0.2216 0.2175 0.2481 0.2784 0.2329 0.2408 0.2567 0.29 0.3091 0.243 0.3425 0.271 0.2739 0.2956 0.3007 0.2898 0.303 0.3137
Eisosomes 0.0006 0.0002 0.0007 0.0009 0.0005 0.0006 0.0007 0.0001 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0003 0.0008 0 0.0001
Endoplasmic Reticulum 0.0014 0.0012 0.0013 0.0003 0.0004 0.0005 0.0048 0.003 0.0032 0.002 0.0013 0.0025 0.003 0.0008 0.0012 0.0023 0.0002 0.0005
Endosome 0.0596 0.0998 0.0526 0.1055 0.0328 0.0339 0.0712 0.1223 0.09 0.0741 0.1035 0.0666 0.0733 0.1042 0.0573 0.034 0.1234 0.0382
Golgi 0.0168 0.024 0.0166 0.0176 0.0159 0.0125 0.0233 0.0249 0.0222 0.0386 0.0223 0.0276 0.0214 0.0197 0.0192 0.0147 0.0192 0.0138
Lipid Particles 0.3278 0.2512 0.2028 0.1836 0.2609 0.2651 0.2237 0.2132 0.1411 0.2124 0.1334 0.2204 0.3049 0.2605 0.2594 0.1698 0.2644 0.2956
Mitochondria 0.004 0.0145 0.0112 0.0094 0.0187 0.0102 0.022 0.0028 0.0115 0.0244 0.0033 0.0283 0.0037 0.0061 0.0085 0.0109 0.0053 0.0124
None 0.0898 0.0346 0.1318 0.0762 0.1114 0.05 0.0655 0.1048 0.0702 0.0801 0.0046 0.0582 0.0519 0.0249 0.0531 0.1327 0.045 0.0495
Nuclear Periphery 0.0032 0.0014 0.0021 0.0003 0.0012 0.0003 0.008 0.0025 0.0019 0.0004 0.0043 0.0012 0.0025 0.001 0.0026 0.0022 0.0006 0.0012
Nucleolus 0.0019 0.0012 0.0011 0.001 0.0003 0.0003 0.0009 0.0004 0.0007 0.0001 0.0003 0.0006 0.0014 0.0013 0.0012 0.0013 0.0004 0.0011
Nucleus 0.0014 0.0007 0.0037 0.0003 0.0002 0.0002 0.0012 0.0008 0.0007 0.0002 0.0005 0.0005 0.001 0.0009 0.0012 0.0013 0.0002 0.0011
Peroxisomes 0.2336 0.303 0.288 0.2789 0.2883 0.3497 0.1764 0.1558 0.2668 0.2771 0.3466 0.2438 0.2047 0.2077 0.2151 0.2809 0.1835 0.2271
Punctate Nuclear 0.0141 0.0097 0.0116 0.0032 0.0125 0.0105 0.0312 0.0179 0.0134 0.0025 0.0147 0.0364 0.0075 0.0193 0.0188 0.0079 0.0448 0.0225
Vacuole 0.002 0.0069 0.0012 0.0055 0.0005 0.0015 0.002 0.0032 0.002 0.0025 0.0027 0.0018 0.0016 0.0039 0.001 0.0018 0.0009 0.0018
Vacuole Periphery 0.0004 0.0013 0.0006 0.0008 0.0005 0.0003 0.0014 0.0006 0.0005 0.0022 0.001 0.0009 0.0005 0.0007 0.0004 0.0006 0.0003 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.7725 3.9709 4.7145 9.4183 3.4556 6.2154 6.6247 5.9617 8.0628 10.6745
Translational Efficiency 0.332 0.7159 0.7079 0.4021 0.7749 0.4994 0.3369 0.5703 0.4196 0.3557

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
Localization
Cell Percentages nucleolus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Pex32

Pex32


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pex32-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available