Standard name | |
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Human Ortholog | |
Description | Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 0 | 3 | 2 |
Bud | 0 | 0 | 0 | 1 | 4 | 2 | 9 | 20 | 12 | 19 | 16 | 19 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 5 | 3 |
Bud Neck | 0 | 0 | 3 | 2 | 6 | 1 | 10 | 13 | 1 | 14 | 3 | 9 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 2 | 1 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 4 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 379 | 77 | 92 | 290 | 345 | 337 | 350 | 510 | 316 | 387 | 239 | 376 | 301 | 428 | 488 | 151 | 70 | 93 | 333 | 83 | 52 | 104 | 276 | 333 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 7 | 7 | 6 | 0 | 0 | 1 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 6 | 0 | 0 | 0 | 3 | 5 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 |
Mitochondria | 12 | 16 | 2 | 36 | 22 | 108 | 151 | 194 | 197 | 225 | 170 | 243 | 1 | 2 | 1 | 10 | 2 | 4 | 10 | 2 | 11 | 7 | 16 | 22 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 5 | 3 | 4 | 2 | 1 | 0 | 1 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 1 | 2 | 18 | 29 | 6 | 15 | 6 | 14 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 0 | 3 | 10 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 1 | 0 | 5 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 1 | 1 | 3 | 6 | 6 |
Unique Cell Count | 388 | 86 | 93 | 299 | 358 | 377 | 434 | 644 | 440 | 526 | 347 | 540 | 306 | 433 | 489 | 157 | 72 | 99 | 375 | 93 | 78 | 124 | 330 | 401 |
Labelled Cell Count | 396 | 97 | 98 | 331 | 380 | 456 | 548 | 776 | 542 | 671 | 441 | 674 | 306 | 435 | 489 | 183 | 83 | 110 | 375 | 93 | 78 | 124 | 330 | 401 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 3.6 | 2.6 | 2.8 | 3.3 | 2.7 | 2.6 | 2.8 | 2.4 | 2.6 | 2.5 | 2.3 | 4.1 | 4.3 | 4.0 | 5.2 | 5.2 | 4.9 | 3.4 | 4.1 | 4.2 |
Std Deviation (1e-4) | 0.6 | 0.7 | 0.6 | 0.9 | 1.3 | 1.2 | 1.2 | 1.1 | 1.2 | 1.4 | 1.5 | 0.6 | 1.5 | 1.4 | 1.1 | 1.6 | 1.0 | 1.3 | 0.7 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.32 | 0.04 | -0.01 | 0.06 | -0.14 | -0.0 | -0.1 | -0.21 | 0.62 | 0.69 | 0.61 | 0.97 | 0.97 | 0.88 | 0.36 | 0.64 | 0.65 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.5395 | -1.0833 | -1.148 | -0.9663 | -1.0445 | -1.19 | -1.3507 | -1.4489 | -1.2261 | -1.3156 | -0.881 | -1.5126 | -0.5129 | 0.3857 | 0.1021 | 0.1109 | -0.4501 | 0.0888 |
Actin | 0.0197 | 0.0042 | 0.0048 | 0.0805 | 0.0395 | 0.0002 | 0.0583 | 0.0008 | 0.0169 | 0.0259 | 0.031 | 0.0062 | 0.0126 | 0.0001 | 0.0014 | 0 | 0.0001 | 0.0028 |
Bud | 0.0006 | 0.0006 | 0.0012 | 0.0089 | 0.0003 | 0 | 0.0018 | 0.0004 | 0.0012 | 0.0004 | 0.0033 | 0.0001 | 0 | 0.0001 | 0.0007 | 0 | 0 | 0.0005 |
Bud Neck | 0.0008 | 0.0001 | 0.0005 | 0.0041 | 0.0018 | 0 | 0.0022 | 0.0001 | 0.0019 | 0.0003 | 0.0014 | 0.0006 | 0 | 0.0001 | 0.0007 | 0 | 0 | 0.0002 |
Bud Periphery | 0.001 | 0.0006 | 0.0023 | 0.0338 | 0.0003 | 0 | 0.0036 | 0.0005 | 0.0024 | 0.0008 | 0.008 | 0.0002 | 0.0001 | 0.0001 | 0.0014 | 0 | 0 | 0.0012 |
Bud Site | 0.0018 | 0.0048 | 0.0082 | 0.012 | 0.0012 | 0 | 0.0077 | 0.0016 | 0.0083 | 0.0011 | 0.0068 | 0.0033 | 0.0001 | 0.0036 | 0.0033 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0168 | 0.0149 | 0.0221 | 0.008 | 0.0066 | 0.0243 | 0.0245 | 0.0421 | 0.0328 | 0.0347 | 0.0372 | 0.0584 | 0.0198 | 0.0248 | 0.0243 | 0.0266 | 0.0136 | 0.0324 |
Cytoplasmic Foci | 0.0281 | 0.0086 | 0.0104 | 0.029 | 0.0228 | 0.0023 | 0.0237 | 0.0113 | 0.0148 | 0.0036 | 0.037 | 0.005 | 0.0014 | 0.0012 | 0.0024 | 0.0007 | 0.0005 | 0.0029 |
Eisosomes | 0.0004 | 0.0003 | 0.0005 | 0.0006 | 0.0002 | 0.0001 | 0.0009 | 0.0003 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0018 | 0.0006 | 0.0008 | 0.0004 | 0.0003 | 0.0003 | 0.0019 | 0.002 | 0.0015 | 0.002 | 0.0021 | 0.0005 | 0.0002 | 0.0002 | 0.0007 | 0.0001 | 0 | 0.0003 |
Endosome | 0.0104 | 0.002 | 0.0023 | 0.0054 | 0.0069 | 0.0001 | 0.0115 | 0.0022 | 0.0142 | 0.0197 | 0.0315 | 0.0042 | 0.0004 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0004 |
Golgi | 0.0031 | 0.0016 | 0.0009 | 0.0037 | 0.0124 | 0 | 0.0074 | 0.0002 | 0.0092 | 0.0107 | 0.0191 | 0.0017 | 0.001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0115 | 0.0018 | 0.0039 | 0.0072 | 0.0435 | 0.0001 | 0.0136 | 0.001 | 0.008 | 0.0025 | 0.0098 | 0.0025 | 0.001 | 0.0001 | 0.0003 | 0 | 0 | 0.0004 |
Mitochondria | 0.0028 | 0.0012 | 0.0013 | 0.0421 | 0.0065 | 0 | 0.0224 | 0.0003 | 0.0123 | 0.06 | 0.0074 | 0.002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0004 |
None | 0.879 | 0.9487 | 0.9295 | 0.7238 | 0.8481 | 0.9719 | 0.7696 | 0.9332 | 0.8532 | 0.8142 | 0.7551 | 0.9072 | 0.9604 | 0.9689 | 0.9596 | 0.9719 | 0.9855 | 0.957 |
Nuclear Periphery | 0.0034 | 0.0067 | 0.0011 | 0.0015 | 0.0002 | 0.0001 | 0.0029 | 0.0011 | 0.0048 | 0.0031 | 0.0038 | 0.0031 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0002 |
Nucleolus | 0.0004 | 0.0001 | 0.0005 | 0.0009 | 0.0004 | 0 | 0.0016 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 |
Nucleus | 0.0006 | 0.0004 | 0.0009 | 0.0011 | 0.0001 | 0.0001 | 0.0022 | 0.0005 | 0.0016 | 0.0003 | 0.0008 | 0.0005 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0 | 0.0003 |
Peroxisomes | 0.0106 | 0.0007 | 0.0055 | 0.0143 | 0.0067 | 0 | 0.0216 | 0.0006 | 0.0093 | 0.0166 | 0.0254 | 0.0017 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0.0002 |
Punctate Nuclear | 0.0055 | 0.0015 | 0.002 | 0.0208 | 0.0009 | 0.0002 | 0.0197 | 0.0008 | 0.0019 | 0.0003 | 0.0016 | 0.0017 | 0.001 | 0.0001 | 0.0012 | 0.0001 | 0 | 0.0003 |
Vacuole | 0.0009 | 0.0001 | 0.0009 | 0.001 | 0.0005 | 0 | 0.0016 | 0.0007 | 0.002 | 0.0012 | 0.0094 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 |
Vacuole Periphery | 0.0007 | 0.0003 | 0.0003 | 0.0007 | 0.0006 | 0 | 0.001 | 0.0001 | 0.0014 | 0.0023 | 0.0088 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 83.9279 | 52.9598 | 86.7841 | 93.9196 | 67.21 | 81.4143 | 89.246 | 94.1172 | 78.9985 | 83.8598 |
Translational Efficiency | 2.905 | 3.9433 | 2.4671 | 2.0334 | 2.9866 | 3.5073 | 2.439 | 3.3155 | 3.4873 | 2.5071 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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