Standard name
Human Ortholog
Description Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.9 0.99 0.97 0.96 0.89 0.81 0.79 0.72 0.74 0.69 0.7 0.98 0.99 1.0 0.96 0.97 0.94 0.89 0.9 0.68 0.84 0.84 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.19 0 0.12 0.06 0.29 0.35 0.3 0.45 0.43 0.49 0.45 0 0 0 0.06 0 0 0 0 0.15 0.06 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 1 0 3 2
Bud 0 0 0 1 4 2 9 20 12 19 16 19 0 0 0 1 0 0 3 0 0 0 5 3
Bud Neck 0 0 3 2 6 1 10 13 1 14 3 9 0 0 0 3 0 0 1 0 0 0 0 2
Bud Site 0 0 0 0 0 2 4 2 1 1 1 3 0 0 0 0 0 0
Cell Periphery 2 1 1 1 0 0 1 0 1 1 1 2 0 1 0 4 1 1 1 0 0 0 0 1
Cytoplasm 379 77 92 290 345 337 350 510 316 387 239 376 301 428 488 151 70 93 333 83 52 104 276 333
Endoplasmic Reticulum 1 0 0 0 0 1 0 1 1 0 1 0 1 1 0 7 7 6 0 0 1 0 1 1
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 2 0 0 1 1 1 6 0 0 0 3 5
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 1 2 0 0 0 2 1 0 0 0 2 0
Mitochondria 12 16 2 36 22 108 151 194 197 225 170 243 1 2 1 10 2 4 10 2 11 7 16 22
Nucleus 0 0 0 0 2 1 1 5 3 4 2 1 0 1 0 3 1 2 0 0 0 0 1 0
Nuclear Periphery 1 1 0 0 0 0 1 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 2 1 1 2 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 2 18 29 6 15 6 14 0 0 0 0 0 0 3 0 3 0 3 10
Vac/Vac Membrane 0 1 0 0 0 1 1 0 3 1 0 5 0 0 0 2 0 1 3 1 1 3 6 6
Unique Cell Count 388 86 93 299 358 377 434 644 440 526 347 540 306 433 489 157 72 99 375 93 78 124 330 401
Labelled Cell Count 396 97 98 331 380 456 548 776 542 671 441 674 306 435 489 183 83 110 375 93 78 124 330 401


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.4 3.6 2.6 2.8 3.3 2.7 2.6 2.8 2.4 2.6 2.5 2.3 4.1 4.3 4.0 5.2 5.2 4.9 3.4 4.1 4.2
Std Deviation (1e-4) 0.6 0.7 0.6 0.9 1.3 1.2 1.2 1.1 1.2 1.4 1.5 0.6 1.5 1.4 1.1 1.6 1.0 1.3 0.7 1.4 1.3
Intensity Change (Log2) 0.08 0.32 0.04 -0.01 0.06 -0.14 -0.0 -0.1 -0.21 0.62 0.69 0.61 0.97 0.97 0.88 0.36 0.64 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.5395 -1.0833 -1.148 -0.9663 -1.0445 -1.19 -1.3507 -1.4489 -1.2261 -1.3156 -0.881 -1.5126 -0.5129 0.3857 0.1021 0.1109 -0.4501 0.0888
Actin 0.0197 0.0042 0.0048 0.0805 0.0395 0.0002 0.0583 0.0008 0.0169 0.0259 0.031 0.0062 0.0126 0.0001 0.0014 0 0.0001 0.0028
Bud 0.0006 0.0006 0.0012 0.0089 0.0003 0 0.0018 0.0004 0.0012 0.0004 0.0033 0.0001 0 0.0001 0.0007 0 0 0.0005
Bud Neck 0.0008 0.0001 0.0005 0.0041 0.0018 0 0.0022 0.0001 0.0019 0.0003 0.0014 0.0006 0 0.0001 0.0007 0 0 0.0002
Bud Periphery 0.001 0.0006 0.0023 0.0338 0.0003 0 0.0036 0.0005 0.0024 0.0008 0.008 0.0002 0.0001 0.0001 0.0014 0 0 0.0012
Bud Site 0.0018 0.0048 0.0082 0.012 0.0012 0 0.0077 0.0016 0.0083 0.0011 0.0068 0.0033 0.0001 0.0036 0.0033 0 0 0.0001
Cell Periphery 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0004 0.0001 0.0006 0.0001 0.0002 0 0 0.0001 0.0001 0 0 0
Cytoplasm 0.0168 0.0149 0.0221 0.008 0.0066 0.0243 0.0245 0.0421 0.0328 0.0347 0.0372 0.0584 0.0198 0.0248 0.0243 0.0266 0.0136 0.0324
Cytoplasmic Foci 0.0281 0.0086 0.0104 0.029 0.0228 0.0023 0.0237 0.0113 0.0148 0.0036 0.037 0.005 0.0014 0.0012 0.0024 0.0007 0.0005 0.0029
Eisosomes 0.0004 0.0003 0.0005 0.0006 0.0002 0.0001 0.0009 0.0003 0.0008 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.0018 0.0006 0.0008 0.0004 0.0003 0.0003 0.0019 0.002 0.0015 0.002 0.0021 0.0005 0.0002 0.0002 0.0007 0.0001 0 0.0003
Endosome 0.0104 0.002 0.0023 0.0054 0.0069 0.0001 0.0115 0.0022 0.0142 0.0197 0.0315 0.0042 0.0004 0.0001 0.0006 0.0001 0 0.0004
Golgi 0.0031 0.0016 0.0009 0.0037 0.0124 0 0.0074 0.0002 0.0092 0.0107 0.0191 0.0017 0.001 0 0.0001 0 0 0.0001
Lipid Particles 0.0115 0.0018 0.0039 0.0072 0.0435 0.0001 0.0136 0.001 0.008 0.0025 0.0098 0.0025 0.001 0.0001 0.0003 0 0 0.0004
Mitochondria 0.0028 0.0012 0.0013 0.0421 0.0065 0 0.0224 0.0003 0.0123 0.06 0.0074 0.002 0.0003 0.0001 0.0004 0.0001 0 0.0004
None 0.879 0.9487 0.9295 0.7238 0.8481 0.9719 0.7696 0.9332 0.8532 0.8142 0.7551 0.9072 0.9604 0.9689 0.9596 0.9719 0.9855 0.957
Nuclear Periphery 0.0034 0.0067 0.0011 0.0015 0.0002 0.0001 0.0029 0.0011 0.0048 0.0031 0.0038 0.0031 0.0003 0.0001 0.0006 0.0001 0 0.0002
Nucleolus 0.0004 0.0001 0.0005 0.0009 0.0004 0 0.0016 0.0001 0.0007 0.0001 0.0002 0.0004 0.0002 0.0001 0.0004 0 0 0.0001
Nucleus 0.0006 0.0004 0.0009 0.0011 0.0001 0.0001 0.0022 0.0005 0.0016 0.0003 0.0008 0.0005 0.0002 0.0001 0.0008 0.0001 0 0.0003
Peroxisomes 0.0106 0.0007 0.0055 0.0143 0.0067 0 0.0216 0.0006 0.0093 0.0166 0.0254 0.0017 0.0005 0 0.0002 0 0 0.0002
Punctate Nuclear 0.0055 0.0015 0.002 0.0208 0.0009 0.0002 0.0197 0.0008 0.0019 0.0003 0.0016 0.0017 0.001 0.0001 0.0012 0.0001 0 0.0003
Vacuole 0.0009 0.0001 0.0009 0.001 0.0005 0 0.0016 0.0007 0.002 0.0012 0.0094 0.0003 0.0001 0.0001 0.0004 0.0001 0 0.0001
Vacuole Periphery 0.0007 0.0003 0.0003 0.0007 0.0006 0 0.001 0.0001 0.0014 0.0023 0.0088 0.0003 0 0 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 83.9279 52.9598 86.7841 93.9196 67.21 81.4143 89.246 94.1172 78.9985 83.8598
Translational Efficiency 2.905 3.9433 2.4671 2.0334 2.9866 3.5073 2.439 3.3155 3.4873 2.5071

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Ump1

Ump1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ump1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available