Standard name | |
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Human Ortholog | |
Description | Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 4 |
Bud | 0 | 0 | 1 | 1 | 0 | 2 | 4 | 5 | 7 | 4 | 12 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 14 |
Bud Neck | 0 | 0 | 3 | 5 | 7 | 5 | 2 | 9 | 2 | 4 | 0 | 6 | 7 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 2 | 7 | 6 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 14 | 27 | 35 | 93 | 151 | 145 | 155 | 143 | 105 | 158 | 101 | 109 | 208 | 190 | 233 | 13 | 20 | 16 | 17 | 31 | 22 | 85 | 145 | 170 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 4 | 4 | 5 | 2 | 0 | 0 | 0 | 1 | 2 | 5 |
Endosome | 0 | 0 | 1 | 2 | 1 | 3 | 3 | 1 | 1 | 0 | 1 | 2 | 1 | 11 | 3 | 0 | 1 | 7 | 2 | 1 | 1 | 3 | 6 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Mitochondria | 22 | 18 | 7 | 18 | 13 | 79 | 162 | 153 | 184 | 180 | 184 | 121 | 1 | 2 | 0 | 4 | 9 | 14 | 9 | 9 | 7 | 8 | 22 | 26 |
Nucleus | 9 | 14 | 33 | 64 | 53 | 83 | 92 | 108 | 87 | 89 | 58 | 55 | 84 | 86 | 84 | 105 | 106 | 85 | 20 | 26 | 50 | 29 | 49 | 65 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 2 | 2 | 4 | 3 | 8 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 2 | 5 | 8 | 7 | 8 | 9 | 16 | 3 | 7 | 2 | 9 | 8 | 5 | 3 | 0 | 6 | 3 | 5 | 9 | 14 | 13 | 32 | 39 |
Vac/Vac Membrane | 5 | 12 | 1 | 2 | 7 | 10 | 12 | 8 | 6 | 12 | 10 | 14 | 6 | 17 | 19 | 5 | 10 | 12 | 18 | 13 | 15 | 15 | 40 | 34 |
Unique Cell Count | 40 | 61 | 77 | 175 | 225 | 294 | 351 | 358 | 304 | 352 | 278 | 258 | 305 | 304 | 339 | 127 | 136 | 120 | 82 | 101 | 132 | 175 | 333 | 408 |
Labelled Cell Count | 50 | 76 | 86 | 193 | 240 | 340 | 445 | 446 | 403 | 461 | 373 | 321 | 320 | 315 | 351 | 133 | 161 | 146 | 82 | 101 | 132 | 175 | 333 | 408 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 4.1 | 4.5 | 4.7 | 4.8 | 3.9 | 3.8 | 4.2 | 3.7 | 3.7 | 3.4 | 3.7 | 4.9 | 5.3 | 5.5 | 8.0 | 7.7 | 7.5 | 4.8 | 5.2 | 5.5 |
Std Deviation (1e-4) | 0.8 | 0.9 | 0.9 | 1.6 | 1.8 | 1.4 | 1.5 | 1.6 | 1.9 | 1.2 | 1.3 | 1.3 | 0.9 | 1.2 | 1.8 | 1.8 | 2.2 | 1.6 | 1.3 | 1.4 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.09 | -0.21 | -0.26 | -0.11 | -0.3 | -0.28 | -0.43 | -0.31 | 0.12 | 0.23 | 0.28 | 0.82 | 0.77 | 0.72 | 0.09 | 0.19 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 2.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.6528 | -0.4047 | -0.2032 | 0.2909 | -0.4089 | -0.2324 | 0.0279 | -0.2383 | -0.0334 | -0.0098 | 0.0558 | -0.1677 | -0.8304 | -0.6172 | -0.6308 | -0.0522 | -0.7319 | -0.475 |
Actin | 0.0629 | 0.0007 | 0.0195 | 0.0226 | 0.0108 | 0.0072 | 0.0347 | 0.0002 | 0.0217 | 0.0193 | 0.0074 | 0.0006 | 0.0047 | 0.0002 | 0.0018 | 0.0001 | 0.0003 | 0.0002 |
Bud | 0.0019 | 0.002 | 0.0123 | 0.0005 | 0.0013 | 0.0009 | 0.0003 | 0.0002 | 0.0041 | 0.0005 | 0.0005 | 0.0002 | 0.0009 | 0.0004 | 0.0004 | 0 | 0.0004 | 0.0001 |
Bud Neck | 0.0062 | 0.0025 | 0.0019 | 0.0021 | 0.0013 | 0.0043 | 0.0018 | 0.0008 | 0.0087 | 0.025 | 0.0022 | 0.0036 | 0.0009 | 0.0006 | 0.0006 | 0.0004 | 0.0014 | 0.0028 |
Bud Periphery | 0.003 | 0.0016 | 0.0049 | 0.0006 | 0.0067 | 0.0008 | 0.0004 | 0.0001 | 0.0012 | 0.0005 | 0.0007 | 0.0003 | 0.0007 | 0.0001 | 0.0003 | 0 | 0.0005 | 0 |
Bud Site | 0.0148 | 0.0044 | 0.016 | 0.0131 | 0.0014 | 0.0009 | 0.0046 | 0.0061 | 0.0081 | 0.0011 | 0.003 | 0.0004 | 0.0011 | 0.0014 | 0.0006 | 0.0001 | 0.0005 | 0.0001 |
Cell Periphery | 0.0008 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0508 | 0.0708 | 0.0414 | 0.0242 | 0.0702 | 0.0498 | 0.0675 | 0.1062 | 0.0777 | 0.0782 | 0.0935 | 0.0664 | 0.0616 | 0.1002 | 0.0587 | 0.023 | 0.067 | 0.0561 |
Cytoplasmic Foci | 0.0553 | 0.0108 | 0.012 | 0.0142 | 0.0167 | 0.019 | 0.0427 | 0.0062 | 0.0122 | 0.0148 | 0.0617 | 0.0039 | 0.0067 | 0.0045 | 0.0043 | 0.0015 | 0.0015 | 0.0029 |
Eisosomes | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0091 | 0.0014 | 0.0026 | 0.002 | 0.0037 | 0.0014 | 0.0029 | 0.0023 | 0.0017 | 0.0016 | 0.0048 | 0.0015 | 0.0015 | 0.0017 | 0.0014 | 0.0003 | 0.0008 | 0.0009 |
Endosome | 0.0588 | 0.0016 | 0.0169 | 0.0146 | 0.0134 | 0.0224 | 0.0155 | 0.0024 | 0.0066 | 0.0102 | 0.0772 | 0.0079 | 0.0026 | 0.0012 | 0.0034 | 0.0006 | 0.0015 | 0.0021 |
Golgi | 0.0165 | 0.0002 | 0.0083 | 0.0129 | 0.0057 | 0.0081 | 0.0067 | 0.0001 | 0.0028 | 0.0089 | 0.0264 | 0.0008 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 |
Lipid Particles | 0.0232 | 0.0005 | 0.0152 | 0.0102 | 0.0296 | 0.0143 | 0.019 | 0.0004 | 0.0033 | 0.0075 | 0.0398 | 0.0017 | 0.0007 | 0.0001 | 0.0006 | 0 | 0.0004 | 0.0002 |
Mitochondria | 0.014 | 0.0013 | 0.0114 | 0.0227 | 0.0242 | 0.0155 | 0.0024 | 0.0005 | 0.0029 | 0.0019 | 0.0163 | 0.0038 | 0.001 | 0.0004 | 0.0008 | 0.0002 | 0.0004 | 0.0003 |
None | 0.1952 | 0.38 | 0.2069 | 0.1068 | 0.2727 | 0.2145 | 0.2106 | 0.278 | 0.2615 | 0.2038 | 0.1601 | 0.265 | 0.1816 | 0.1599 | 0.176 | 0.0624 | 0.194 | 0.1473 |
Nuclear Periphery | 0.0459 | 0.0124 | 0.0176 | 0.0154 | 0.0254 | 0.0096 | 0.008 | 0.0119 | 0.0077 | 0.0108 | 0.0242 | 0.0073 | 0.0129 | 0.0079 | 0.0138 | 0.0045 | 0.0068 | 0.0056 |
Nucleolus | 0.0097 | 0.0103 | 0.0085 | 0.0073 | 0.0068 | 0.0302 | 0.0061 | 0.0098 | 0.0077 | 0.0076 | 0.0082 | 0.0168 | 0.0112 | 0.0121 | 0.0126 | 0.0107 | 0.035 | 0.0267 |
Nucleus | 0.3424 | 0.4329 | 0.5118 | 0.6624 | 0.3622 | 0.506 | 0.5124 | 0.5271 | 0.5157 | 0.4044 | 0.3517 | 0.5502 | 0.6615 | 0.6447 | 0.6594 | 0.8511 | 0.6098 | 0.6825 |
Peroxisomes | 0.0163 | 0.002 | 0.0133 | 0.0188 | 0.0541 | 0.0062 | 0.0238 | 0.0002 | 0.0044 | 0.0042 | 0.0407 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0626 | 0.0625 | 0.0733 | 0.048 | 0.0891 | 0.0818 | 0.0385 | 0.0451 | 0.05 | 0.1987 | 0.0739 | 0.064 | 0.0477 | 0.063 | 0.0631 | 0.0447 | 0.0784 | 0.0712 |
Vacuole | 0.0065 | 0.0014 | 0.0038 | 0.0007 | 0.0019 | 0.0051 | 0.001 | 0.0019 | 0.0013 | 0.0007 | 0.0053 | 0.0039 | 0.0013 | 0.0013 | 0.001 | 0.0002 | 0.0009 | 0.0008 |
Vacuole Periphery | 0.0034 | 0.0003 | 0.0014 | 0.0005 | 0.002 | 0.0015 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0018 | 0.0012 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.1311 | 13.5263 | 15.4416 | 19.8158 | 9.809 | 17.4493 | 19.4625 | 18.4613 | 20.0145 | 21.5343 |
Translational Efficiency | 0.9382 | 0.7214 | 0.7389 | 0.4789 | 1.1764 | 0.5872 | 0.5551 | 0.7059 | 0.6662 | 0.5296 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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