Standard name | |
---|---|
Human Ortholog | |
Description | Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 7 | 0 | 4 | 0 | 2 | 6 | 5 | 4 | 6 | 14 | 12 |
Bud | 3 | 2 | 5 | 3 | 6 | 3 | 6 | 8 | 9 | 11 | 14 | 10 | 5 | 2 | 0 | 12 | 3 | 10 | 1 | 2 | 1 | 1 | 4 | 11 |
Bud Neck | 1 | 3 | 13 | 6 | 20 | 15 | 13 | 16 | 12 | 12 | 8 | 13 | 11 | 14 | 8 | 0 | 0 | 1 | 1 | 5 | 1 | 3 | 5 | 11 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 7 | 6 | 5 | 2 | 1 | 0 | 0 | 0 | 2 | 3 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 3 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 1 | 4 | 0 | 2 | 2 | 7 | 0 | 0 | 1 | 3 | 6 | 6 |
Cytoplasm | 90 | 138 | 99 | 158 | 269 | 234 | 252 | 323 | 307 | 230 | 181 | 188 | 328 | 394 | 503 | 9 | 3 | 5 | 50 | 90 | 42 | 104 | 211 | 221 |
Endoplasmic Reticulum | 4 | 2 | 1 | 1 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 2 | 4 | 3 |
Endosome | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 7 | 20 | 19 | 19 | 7 | 7 | 9 | 10 | 11 | 12 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 2 | 2 | 2 | 0 | 1 | 0 | 1 | 2 |
Mitochondria | 65 | 37 | 4 | 10 | 13 | 48 | 127 | 83 | 156 | 117 | 157 | 111 | 4 | 0 | 1 | 15 | 9 | 8 | 65 | 62 | 54 | 101 | 134 | 113 |
Nucleus | 0 | 0 | 2 | 1 | 2 | 0 | 2 | 2 | 4 | 1 | 1 | 3 | 1 | 2 | 0 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 19 | 22 | 30 | 1 | 1 | 2 | 0 | 5 | 3 |
SpindlePole | 2 | 3 | 3 | 9 | 27 | 16 | 27 | 29 | 23 | 16 | 9 | 21 | 16 | 19 | 12 | 26 | 29 | 23 | 6 | 6 | 6 | 9 | 36 | 36 |
Vac/Vac Membrane | 0 | 3 | 1 | 0 | 6 | 3 | 1 | 0 | 2 | 1 | 0 | 1 | 3 | 2 | 4 | 7 | 14 | 14 | 6 | 10 | 3 | 2 | 12 | 15 |
Unique Cell Count | 141 | 173 | 112 | 184 | 324 | 299 | 372 | 416 | 436 | 328 | 296 | 294 | 356 | 430 | 528 | 93 | 79 | 103 | 157 | 197 | 136 | 260 | 468 | 465 |
Labelled Cell Count | 167 | 191 | 131 | 193 | 352 | 326 | 430 | 470 | 522 | 396 | 377 | 354 | 375 | 449 | 536 | 122 | 109 | 136 | 157 | 197 | 136 | 260 | 468 | 465 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 2.8 | 3.8 | 4.4 | 4.2 | 4.4 | 3.5 | 3.2 | 3.5 | 2.9 | 3.1 | 2.8 | 3.2 | 4.7 | 4.8 | 4.9 | 6.4 | 6.8 | 6.7 | 4.6 | 5.0 | 5.0 |
Std Deviation (1e-4) | 0.4 | 0.6 | 1.2 | 1.6 | 1.4 | 0.8 | 1.2 | 0.9 | 0.7 | 1.0 | 0.8 | 1.1 | 1.0 | 1.0 | 1.2 | 1.3 | 1.1 | 1.6 | 1.2 | 4.8 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.01 | -0.34 | -0.48 | -0.33 | -0.6 | -0.51 | -0.67 | -0.44 | 0.1 | 0.12 | 0.16 | 0.53 | 0.63 | 0.61 | 0.05 | 0.18 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 4.3 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0796 | -0.5165 | -0.7898 | -0.6759 | -0.9989 | -0.5823 | 0.0332 | -0.105 | -0.0916 | -0.6501 | -0.501 | -0.5152 | -1.1978 | -0.8956 | -1.0201 | -1.215 | -1.2648 | -0.9317 |
Actin | 0.0123 | 0.0106 | 0.0176 | 0.0029 | 0.0415 | 0.0107 | 0.0634 | 0.0077 | 0.0107 | 0.0027 | 0.0099 | 0.011 | 0.0123 | 0.0174 | 0.0073 | 0.0102 | 0.0154 | 0.0084 |
Bud | 0.0008 | 0.0069 | 0.0025 | 0.002 | 0.0019 | 0.0016 | 0.003 | 0.0021 | 0.0074 | 0.0021 | 0.0033 | 0.0024 | 0.0043 | 0.0021 | 0.0072 | 0.0033 | 0.0027 | 0.0027 |
Bud Neck | 0.0011 | 0.0022 | 0.0036 | 0.005 | 0.0028 | 0.0084 | 0.0067 | 0.003 | 0.006 | 0.0013 | 0.0008 | 0.0165 | 0.0018 | 0.0029 | 0.0028 | 0.0024 | 0.001 | 0.0086 |
Bud Periphery | 0.0032 | 0.0083 | 0.0038 | 0.0052 | 0.0068 | 0.0022 | 0.0037 | 0.0049 | 0.0135 | 0.0084 | 0.0133 | 0.0047 | 0.0047 | 0.0039 | 0.0062 | 0.0083 | 0.0067 | 0.0027 |
Bud Site | 0.0024 | 0.0384 | 0.0079 | 0.0054 | 0.0122 | 0.0009 | 0.015 | 0.0239 | 0.0118 | 0.0038 | 0.0036 | 0.0032 | 0.0068 | 0.0266 | 0.0123 | 0.0147 | 0.0017 | 0.0007 |
Cell Periphery | 0.0011 | 0.0018 | 0.0011 | 0.0005 | 0.0007 | 0.0009 | 0.0028 | 0.0015 | 0.0021 | 0.0026 | 0.0006 | 0.0007 | 0.0012 | 0.0018 | 0.0035 | 0.0008 | 0.0005 | 0.0005 |
Cytoplasm | 0.0231 | 0.0361 | 0.0441 | 0.0358 | 0.0168 | 0.0307 | 0.0317 | 0.0531 | 0.0407 | 0.0408 | 0.0292 | 0.0521 | 0.0353 | 0.047 | 0.0366 | 0.0302 | 0.0525 | 0.0488 |
Cytoplasmic Foci | 0.1454 | 0.1434 | 0.1344 | 0.1212 | 0.1348 | 0.1315 | 0.1559 | 0.1629 | 0.1129 | 0.0859 | 0.1268 | 0.1401 | 0.1419 | 0.1453 | 0.1362 | 0.1163 | 0.1393 | 0.1455 |
Eisosomes | 0.0013 | 0.0006 | 0.0006 | 0.0003 | 0.0011 | 0.0007 | 0.0017 | 0.001 | 0.001 | 0.001 | 0.0007 | 0.0004 | 0.0012 | 0.002 | 0.0017 | 0.0007 | 0.0004 | 0.0005 |
Endoplasmic Reticulum | 0.0043 | 0.0018 | 0.0022 | 0.0044 | 0.0016 | 0.0016 | 0.004 | 0.0041 | 0.0033 | 0.0019 | 0.001 | 0.0016 | 0.0087 | 0.0045 | 0.0021 | 0.0027 | 0.0032 | 0.0033 |
Endosome | 0.1301 | 0.115 | 0.1057 | 0.0341 | 0.075 | 0.0749 | 0.1336 | 0.0767 | 0.0815 | 0.048 | 0.0808 | 0.0771 | 0.1461 | 0.1356 | 0.1243 | 0.0765 | 0.2014 | 0.11 |
Golgi | 0.0351 | 0.0505 | 0.0315 | 0.0339 | 0.0141 | 0.0354 | 0.0469 | 0.0402 | 0.0319 | 0.0311 | 0.0351 | 0.036 | 0.0619 | 0.0759 | 0.0477 | 0.0414 | 0.0742 | 0.0613 |
Lipid Particles | 0.0489 | 0.0279 | 0.0526 | 0.0207 | 0.0531 | 0.0452 | 0.0779 | 0.0307 | 0.0304 | 0.0036 | 0.0491 | 0.0426 | 0.0669 | 0.0376 | 0.036 | 0.0309 | 0.0095 | 0.0352 |
Mitochondria | 0.216 | 0.289 | 0.2908 | 0.4283 | 0.4191 | 0.3107 | 0.1208 | 0.2086 | 0.2952 | 0.2254 | 0.3205 | 0.2166 | 0.2673 | 0.2555 | 0.3036 | 0.3824 | 0.1998 | 0.3637 |
None | 0.2743 | 0.1499 | 0.1856 | 0.2277 | 0.0818 | 0.195 | 0.2243 | 0.2664 | 0.2229 | 0.4529 | 0.2136 | 0.2653 | 0.1364 | 0.1455 | 0.1699 | 0.1281 | 0.1827 | 0.1063 |
Nuclear Periphery | 0.0015 | 0.0008 | 0.0012 | 0.0024 | 0.0012 | 0.0025 | 0.0084 | 0.0022 | 0.0059 | 0.001 | 0.0007 | 0.0018 | 0.0048 | 0.0018 | 0.0014 | 0.0031 | 0.0014 | 0.0008 |
Nucleolus | 0.0013 | 0.0023 | 0.0015 | 0.001 | 0.0029 | 0.0016 | 0.0047 | 0.0014 | 0.0022 | 0.0025 | 0.0009 | 0.0025 | 0.0042 | 0.0017 | 0.0024 | 0.0008 | 0.0005 | 0.0006 |
Nucleus | 0.001 | 0.0024 | 0.0014 | 0.0015 | 0.0016 | 0.0016 | 0.0028 | 0.0013 | 0.0044 | 0.0035 | 0.001 | 0.0045 | 0.002 | 0.002 | 0.0019 | 0.0008 | 0.001 | 0.0009 |
Peroxisomes | 0.0843 | 0.0961 | 0.0943 | 0.0569 | 0.1172 | 0.1278 | 0.0699 | 0.0957 | 0.0969 | 0.0723 | 0.0963 | 0.0983 | 0.0726 | 0.0766 | 0.081 | 0.1361 | 0.0873 | 0.0878 |
Punctate Nuclear | 0.0017 | 0.0049 | 0.005 | 0.0023 | 0.0033 | 0.0083 | 0.0088 | 0.0028 | 0.0041 | 0.0031 | 0.0021 | 0.0095 | 0.0041 | 0.0023 | 0.0029 | 0.0024 | 0.0008 | 0.0026 |
Vacuole | 0.0084 | 0.008 | 0.0091 | 0.0058 | 0.0069 | 0.0051 | 0.011 | 0.0077 | 0.0111 | 0.0044 | 0.0074 | 0.0092 | 0.0114 | 0.0094 | 0.0097 | 0.0042 | 0.0133 | 0.0054 |
Vacuole Periphery | 0.0024 | 0.0029 | 0.0035 | 0.0027 | 0.0036 | 0.0026 | 0.0028 | 0.0021 | 0.0043 | 0.0016 | 0.0034 | 0.004 | 0.004 | 0.0026 | 0.0035 | 0.0034 | 0.0049 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.5135 | 7.3532 | 17.9281 | 14.516 | 12.9502 | 12.9188 | 10.8867 | 18.6382 | 14.5935 | 14.4938 |
Translational Efficiency | 1.4358 | 2.2295 | 0.8954 | 1.3591 | 1.3836 | 1.5858 | 2.1412 | 0.9493 | 1.3195 | 1.3624 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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