Standard name
Human Ortholog
Description Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0.06 0 0.14 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.13 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.09 0.06 0.07 0.07 0.09 0.06 0.06 0 0 0.24 0.24 0.41 0.32 0.27 0.18 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0.05 0.08 0.11 0 0 0 0 0 0
Endosome 0.06 0 0.12 0 0.1 0 0 0 0 0 0 0 0.1 0.07 0.07 0.13 0.11 0.08 0.06 0.06 0.07 0.08 0.11 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.08 0 0.08 0.13 0.06 0.08 0 0.06 0.07 0
Mitochondria 0.83 0.89 0.77 0.88 0.81 0.91 0.9 0.89 0.93 0.95 0.96 0.97 0.39 0.4 0.12 0.18 0.13 0.14 0.73 0.68 0.74 0.65 0.55 0.56
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.05 0 0.11 0 0.05 0 0 0 0 0 0 0 0.05 0.06 0 0.13 0.05 0 0.05 0 0.05 0.07 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.07 0.16 0.15 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.14 0.1 0 0.15 0.05 0.05 0.05 0.07 0.06 0.05 0.05 0.18 0.22 0.17 0.07 0.07 0.17 0 0.09 0 0.06 0.11 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 6 0 0 0 0 0 0 0 0 0 0 3 2 4 6 6 5 11 5 5 1 2 3
Bud 11 3 3 7 8 14 15 15 17 15 15 20 1 1 0 14 4 10 3 0 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 2 0 1 1 3 7
Bud Site 1 0 0 1 2 8 4 6 5 7 7 2 0 0 0 1 0 0
Cell Periphery 17 7 12 3 0 2 0 0 0 1 1 5 2 2 3 13 19 4 1 0 1 0 0 0
Cytoplasm 11 8 15 16 17 24 31 39 20 26 9 7 20 20 24 77 41 13 0 0 0 0 0 0
Endoplasmic Reticulum 7 4 2 0 0 2 1 1 5 1 7 4 5 3 3 12 12 8 1 1 0 0 0 1
Endosome 30 9 34 8 26 3 2 4 2 1 1 1 8 6 4 31 16 6 27 14 22 8 15 14
Golgi 9 5 2 0 2 0 0 0 0 0 0 0 7 7 5 3 12 9 31 18 13 7 9 7
Mitochondria 389 194 222 158 215 302 413 387 303 425 333 384 33 33 7 43 20 10 367 159 232 74 77 106
Nucleus 11 0 3 0 2 10 10 17 8 6 10 5 1 1 1 4 8 2 1 1 0 0 1 0
Nuclear Periphery 4 2 2 0 1 1 3 3 4 4 4 4 0 0 0 0 0 0 2 1 2 0 1 1
Nucleolus 2 0 3 0 1 4 8 6 3 3 3 7 0 0 0 0 0 0 3 1 1 0 1 3
Peroxisomes 23 1 33 6 14 7 6 12 1 2 1 3 4 5 0 31 7 0 25 6 15 7 5 5
SpindlePole 15 0 14 7 8 9 17 12 13 6 4 9 2 2 4 39 22 5 1 1 2 1 3 2
Vac/Vac Membrane 90 30 30 4 39 17 25 20 24 27 18 20 15 18 10 16 10 12 21 21 6 6 15 28
Unique Cell Count 468 219 287 179 264 332 457 436 327 446 348 395 84 83 59 243 151 71 504 236 312 114 141 190
Labelled Cell Count 625 269 375 210 335 403 535 522 405 524 413 471 101 100 65 290 179 84 504 236 312 114 141 190


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 5.9 5.2 4.8 4.6 3.7 3.4 3.7 3.3 3.3 3.3 3.5 6.7 6.7 6.7 5.1 5.4 6.0 6.3 6.7 6.9
Std Deviation (1e-4) 1.1 0.9 1.4 1.7 1.6 1.9 1.6 1.2 1.0 1.2 0.8 1.0 1.5 1.5 0.9 1.0 0.9 1.2 1.3 1.5 1.5
Intensity Change (Log2) -0.12 -0.18 -0.5 -0.59 -0.47 -0.66 -0.65 -0.65 -0.58 0.37 0.37 0.36 -0.02 0.06 0.22 0.27 0.37 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.6 3.3 0
Cytoplasm 1.6 0.6 1.0 0.9 1.9 0.5 0.3 -1.7 -2.5 5.1 5.2 7.8 8.0 6.5 3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0
Endosome 0 0 0 0 0 0 0 0 0 -0.6 -1.2 -1.1 0.3 -0.4 -0.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -6.6 -6.5 -9.7 -13.7 -12.8 -10.0
Nucleus 0 0 1.7 1.2 2.3 1.3 0 1.6 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 -1.8 -1.4 -2.7 0.4 -2.4 -3.0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 4.3 3.5 0
Vacuole -3.3 1.5 -2.5 -2.5 -3.0 -1.4 -2.2 -2.5 -2.7 1.8 2.7 1.4 -1.6 -1.3 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.761 1.9613 1.631 1.3042 1.7073 1.733 4.2183 4.3852 4.1111 3.0193 4.6144 4.5173 4.1252 4.1099 4.2548 4.0453 4.142 4.2506
Actin 0.0097 0.0152 0.0174 0.0022 0.0078 0.0038 0.0228 0.037 0.0337 0.015 0.0486 0.0325 0.006 0.0063 0.0112 0.0092 0.0034 0.0068
Bud 0.0012 0.0054 0.0077 0.002 0.007 0.0007 0.0017 0.011 0.003 0.0046 0.0074 0.0015 0.002 0.0104 0.0053 0.0027 0.0029 0.0013
Bud Neck 0.0021 0.0082 0.0026 0.0311 0.0047 0.0026 0.0082 0.0033 0.006 0.0107 0.0013 0.0181 0.0041 0.001 0.0033 0.0038 0.0005 0.0116
Bud Periphery 0.0019 0.0088 0.0178 0.006 0.0081 0.0018 0.0043 0.019 0.0134 0.0163 0.0538 0.0044 0.0052 0.0138 0.0135 0.0109 0.0114 0.0038
Bud Site 0.0077 0.0817 0.0267 0.0114 0.0633 0.0011 0.0357 0.0824 0.0161 0.0093 0.0058 0.002 0.0089 0.0257 0.0279 0.0056 0.0016 0.0018
Cell Periphery 0.0015 0.0025 0.0044 0.001 0.0009 0.0003 0.008 0.0055 0.0067 0.0064 0.0042 0.0048 0.0011 0.0022 0.0027 0.0008 0.0029 0.0012
Cytoplasm 0.0214 0.0472 0.0406 0.0044 0.0172 0.0146 0.0305 0.0534 0.0294 0.0379 0.047 0.034 0.016 0.0501 0.0251 0.0092 0.0148 0.0159
Cytoplasmic Foci 0.0537 0.0643 0.0596 0.03 0.1234 0.0341 0.0835 0.0851 0.0607 0.0575 0.0412 0.0722 0.0569 0.0842 0.0421 0.0584 0.0265 0.0435
Eisosomes 0.0004 0.0005 0.0006 0.0001 0.0001 0.0002 0.0007 0.0013 0.0021 0.0008 0.005 0.0007 0.0005 0.0003 0.0009 0.0004 0.0003 0.0004
Endoplasmic Reticulum 0.0036 0.0084 0.0029 0.0015 0.0018 0.0012 0.0018 0.0019 0.0021 0.0011 0.0011 0.0036 0.002 0.0021 0.0019 0.0004 0.0008 0.0008
Endosome 0.2185 0.137 0.1172 0.0906 0.1667 0.0981 0.1021 0.1276 0.1088 0.1024 0.0807 0.1023 0.1275 0.1334 0.0689 0.0683 0.0787 0.0837
Golgi 0.0801 0.0903 0.0773 0.0742 0.0956 0.098 0.0462 0.089 0.0754 0.0394 0.0397 0.1004 0.0752 0.069 0.0419 0.044 0.055 0.079
Lipid Particles 0.0374 0.0091 0.0073 0.006 0.0231 0.0082 0.0155 0.0122 0.0086 0.0056 0.0131 0.0207 0.012 0.0091 0.0053 0.0064 0.0121 0.0082
Mitochondria 0.4837 0.4389 0.5337 0.6769 0.3642 0.6918 0.5106 0.3966 0.5562 0.6262 0.4929 0.5345 0.6106 0.5179 0.6434 0.7242 0.7568 0.6828
None 0.0044 0.0129 0.0162 0.0011 0.002 0.0121 0.0179 0.0155 0.0328 0.0025 0.1008 0.0096 0.0237 0.0142 0.0447 0.0063 0.0097 0.0199
Nuclear Periphery 0.0056 0.002 0.0008 0.0006 0.0012 0.0002 0.0042 0.0004 0.0005 0.0002 0.0003 0.0017 0.0008 0.0009 0.0004 0.0001 0.0006 0.0002
Nucleolus 0.0019 0.0026 0.0009 0.0005 0.0014 0.0003 0.0019 0.0007 0.0006 0.0005 0.0004 0.0007 0.001 0.0015 0.001 0.0003 0.0003 0.0004
Nucleus 0.0017 0.0026 0.0015 0.0008 0.0017 0.0004 0.0026 0.0009 0.0009 0.0008 0.0004 0.0007 0.0008 0.0025 0.0009 0.0004 0.0004 0.0006
Peroxisomes 0.0356 0.0361 0.0276 0.0425 0.084 0.0214 0.0462 0.0443 0.0279 0.0445 0.0493 0.041 0.0305 0.0319 0.0473 0.0397 0.0104 0.0296
Punctate Nuclear 0.0017 0.0027 0.0007 0.0001 0.0017 0.0002 0.011 0.0004 0.0004 0.0003 0.0009 0.0007 0.0006 0.0006 0.0004 0.0002 0.0001 0.0006
Vacuole 0.0181 0.0175 0.0278 0.009 0.0202 0.0044 0.0295 0.0094 0.0105 0.0113 0.0035 0.0092 0.0099 0.0176 0.0083 0.0051 0.0069 0.0048
Vacuole Periphery 0.0082 0.0061 0.0087 0.0078 0.0039 0.0043 0.0152 0.0033 0.0043 0.0067 0.0026 0.0046 0.0048 0.0053 0.0035 0.0036 0.0041 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3122 12.6754 16.5312 17.9308 12.256 8.5887 16.2143 20.1833 18.8818 17.2013
Translational Efficiency 0.9314 0.8622 0.7145 0.6994 0.6693 1.2383 0.7742 0.6072 0.6475 0.8038

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
Localization
Cell Percentages mitochondrion (79%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Fzo1

Fzo1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fzo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available