Standard name | |
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Human Ortholog | |
Description | Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 4 | 6 | 6 | 5 | 11 | 5 | 5 | 1 | 2 | 3 |
Bud | 11 | 3 | 3 | 7 | 8 | 14 | 15 | 15 | 17 | 15 | 15 | 20 | 1 | 1 | 0 | 14 | 4 | 10 | 3 | 0 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 1 | 1 | 3 | 7 |
Bud Site | 1 | 0 | 0 | 1 | 2 | 8 | 4 | 6 | 5 | 7 | 7 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 17 | 7 | 12 | 3 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 5 | 2 | 2 | 3 | 13 | 19 | 4 | 1 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 11 | 8 | 15 | 16 | 17 | 24 | 31 | 39 | 20 | 26 | 9 | 7 | 20 | 20 | 24 | 77 | 41 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 7 | 4 | 2 | 0 | 0 | 2 | 1 | 1 | 5 | 1 | 7 | 4 | 5 | 3 | 3 | 12 | 12 | 8 | 1 | 1 | 0 | 0 | 0 | 1 |
Endosome | 30 | 9 | 34 | 8 | 26 | 3 | 2 | 4 | 2 | 1 | 1 | 1 | 8 | 6 | 4 | 31 | 16 | 6 | 27 | 14 | 22 | 8 | 15 | 14 |
Golgi | 9 | 5 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 7 | 5 | 3 | 12 | 9 | 31 | 18 | 13 | 7 | 9 | 7 |
Mitochondria | 389 | 194 | 222 | 158 | 215 | 302 | 413 | 387 | 303 | 425 | 333 | 384 | 33 | 33 | 7 | 43 | 20 | 10 | 367 | 159 | 232 | 74 | 77 | 106 |
Nucleus | 11 | 0 | 3 | 0 | 2 | 10 | 10 | 17 | 8 | 6 | 10 | 5 | 1 | 1 | 1 | 4 | 8 | 2 | 1 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 4 | 2 | 2 | 0 | 1 | 1 | 3 | 3 | 4 | 4 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 1 | 1 |
Nucleolus | 2 | 0 | 3 | 0 | 1 | 4 | 8 | 6 | 3 | 3 | 3 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 1 | 3 |
Peroxisomes | 23 | 1 | 33 | 6 | 14 | 7 | 6 | 12 | 1 | 2 | 1 | 3 | 4 | 5 | 0 | 31 | 7 | 0 | 25 | 6 | 15 | 7 | 5 | 5 |
SpindlePole | 15 | 0 | 14 | 7 | 8 | 9 | 17 | 12 | 13 | 6 | 4 | 9 | 2 | 2 | 4 | 39 | 22 | 5 | 1 | 1 | 2 | 1 | 3 | 2 |
Vac/Vac Membrane | 90 | 30 | 30 | 4 | 39 | 17 | 25 | 20 | 24 | 27 | 18 | 20 | 15 | 18 | 10 | 16 | 10 | 12 | 21 | 21 | 6 | 6 | 15 | 28 |
Unique Cell Count | 468 | 219 | 287 | 179 | 264 | 332 | 457 | 436 | 327 | 446 | 348 | 395 | 84 | 83 | 59 | 243 | 151 | 71 | 504 | 236 | 312 | 114 | 141 | 190 |
Labelled Cell Count | 625 | 269 | 375 | 210 | 335 | 403 | 535 | 522 | 405 | 524 | 413 | 471 | 101 | 100 | 65 | 290 | 179 | 84 | 504 | 236 | 312 | 114 | 141 | 190 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.9 | 5.2 | 4.8 | 4.6 | 3.7 | 3.4 | 3.7 | 3.3 | 3.3 | 3.3 | 3.5 | 6.7 | 6.7 | 6.7 | 5.1 | 5.4 | 6.0 | 6.3 | 6.7 | 6.9 |
Std Deviation (1e-4) | 1.1 | 0.9 | 1.4 | 1.7 | 1.6 | 1.9 | 1.6 | 1.2 | 1.0 | 1.2 | 0.8 | 1.0 | 1.5 | 1.5 | 0.9 | 1.0 | 0.9 | 1.2 | 1.3 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.18 | -0.5 | -0.59 | -0.47 | -0.66 | -0.65 | -0.65 | -0.58 | 0.37 | 0.37 | 0.36 | -0.02 | 0.06 | 0.22 | 0.27 | 0.37 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 3.3 | 0 |
Cytoplasm | 1.6 | 0.6 | 1.0 | 0.9 | 1.9 | 0.5 | 0.3 | -1.7 | -2.5 | 5.1 | 5.2 | 7.8 | 8.0 | 6.5 | 3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -1.2 | -1.1 | 0.3 | -0.4 | -0.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.6 | -6.5 | -9.7 | -13.7 | -12.8 | -10.0 |
Nucleus | 0 | 0 | 1.7 | 1.2 | 2.3 | 1.3 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | -1.4 | -2.7 | 0.4 | -2.4 | -3.0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 3.5 | 0 |
Vacuole | -3.3 | 1.5 | -2.5 | -2.5 | -3.0 | -1.4 | -2.2 | -2.5 | -2.7 | 1.8 | 2.7 | 1.4 | -1.6 | -1.3 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.761 | 1.9613 | 1.631 | 1.3042 | 1.7073 | 1.733 | 4.2183 | 4.3852 | 4.1111 | 3.0193 | 4.6144 | 4.5173 | 4.1252 | 4.1099 | 4.2548 | 4.0453 | 4.142 | 4.2506 |
Actin | 0.0097 | 0.0152 | 0.0174 | 0.0022 | 0.0078 | 0.0038 | 0.0228 | 0.037 | 0.0337 | 0.015 | 0.0486 | 0.0325 | 0.006 | 0.0063 | 0.0112 | 0.0092 | 0.0034 | 0.0068 |
Bud | 0.0012 | 0.0054 | 0.0077 | 0.002 | 0.007 | 0.0007 | 0.0017 | 0.011 | 0.003 | 0.0046 | 0.0074 | 0.0015 | 0.002 | 0.0104 | 0.0053 | 0.0027 | 0.0029 | 0.0013 |
Bud Neck | 0.0021 | 0.0082 | 0.0026 | 0.0311 | 0.0047 | 0.0026 | 0.0082 | 0.0033 | 0.006 | 0.0107 | 0.0013 | 0.0181 | 0.0041 | 0.001 | 0.0033 | 0.0038 | 0.0005 | 0.0116 |
Bud Periphery | 0.0019 | 0.0088 | 0.0178 | 0.006 | 0.0081 | 0.0018 | 0.0043 | 0.019 | 0.0134 | 0.0163 | 0.0538 | 0.0044 | 0.0052 | 0.0138 | 0.0135 | 0.0109 | 0.0114 | 0.0038 |
Bud Site | 0.0077 | 0.0817 | 0.0267 | 0.0114 | 0.0633 | 0.0011 | 0.0357 | 0.0824 | 0.0161 | 0.0093 | 0.0058 | 0.002 | 0.0089 | 0.0257 | 0.0279 | 0.0056 | 0.0016 | 0.0018 |
Cell Periphery | 0.0015 | 0.0025 | 0.0044 | 0.001 | 0.0009 | 0.0003 | 0.008 | 0.0055 | 0.0067 | 0.0064 | 0.0042 | 0.0048 | 0.0011 | 0.0022 | 0.0027 | 0.0008 | 0.0029 | 0.0012 |
Cytoplasm | 0.0214 | 0.0472 | 0.0406 | 0.0044 | 0.0172 | 0.0146 | 0.0305 | 0.0534 | 0.0294 | 0.0379 | 0.047 | 0.034 | 0.016 | 0.0501 | 0.0251 | 0.0092 | 0.0148 | 0.0159 |
Cytoplasmic Foci | 0.0537 | 0.0643 | 0.0596 | 0.03 | 0.1234 | 0.0341 | 0.0835 | 0.0851 | 0.0607 | 0.0575 | 0.0412 | 0.0722 | 0.0569 | 0.0842 | 0.0421 | 0.0584 | 0.0265 | 0.0435 |
Eisosomes | 0.0004 | 0.0005 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0013 | 0.0021 | 0.0008 | 0.005 | 0.0007 | 0.0005 | 0.0003 | 0.0009 | 0.0004 | 0.0003 | 0.0004 |
Endoplasmic Reticulum | 0.0036 | 0.0084 | 0.0029 | 0.0015 | 0.0018 | 0.0012 | 0.0018 | 0.0019 | 0.0021 | 0.0011 | 0.0011 | 0.0036 | 0.002 | 0.0021 | 0.0019 | 0.0004 | 0.0008 | 0.0008 |
Endosome | 0.2185 | 0.137 | 0.1172 | 0.0906 | 0.1667 | 0.0981 | 0.1021 | 0.1276 | 0.1088 | 0.1024 | 0.0807 | 0.1023 | 0.1275 | 0.1334 | 0.0689 | 0.0683 | 0.0787 | 0.0837 |
Golgi | 0.0801 | 0.0903 | 0.0773 | 0.0742 | 0.0956 | 0.098 | 0.0462 | 0.089 | 0.0754 | 0.0394 | 0.0397 | 0.1004 | 0.0752 | 0.069 | 0.0419 | 0.044 | 0.055 | 0.079 |
Lipid Particles | 0.0374 | 0.0091 | 0.0073 | 0.006 | 0.0231 | 0.0082 | 0.0155 | 0.0122 | 0.0086 | 0.0056 | 0.0131 | 0.0207 | 0.012 | 0.0091 | 0.0053 | 0.0064 | 0.0121 | 0.0082 |
Mitochondria | 0.4837 | 0.4389 | 0.5337 | 0.6769 | 0.3642 | 0.6918 | 0.5106 | 0.3966 | 0.5562 | 0.6262 | 0.4929 | 0.5345 | 0.6106 | 0.5179 | 0.6434 | 0.7242 | 0.7568 | 0.6828 |
None | 0.0044 | 0.0129 | 0.0162 | 0.0011 | 0.002 | 0.0121 | 0.0179 | 0.0155 | 0.0328 | 0.0025 | 0.1008 | 0.0096 | 0.0237 | 0.0142 | 0.0447 | 0.0063 | 0.0097 | 0.0199 |
Nuclear Periphery | 0.0056 | 0.002 | 0.0008 | 0.0006 | 0.0012 | 0.0002 | 0.0042 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0017 | 0.0008 | 0.0009 | 0.0004 | 0.0001 | 0.0006 | 0.0002 |
Nucleolus | 0.0019 | 0.0026 | 0.0009 | 0.0005 | 0.0014 | 0.0003 | 0.0019 | 0.0007 | 0.0006 | 0.0005 | 0.0004 | 0.0007 | 0.001 | 0.0015 | 0.001 | 0.0003 | 0.0003 | 0.0004 |
Nucleus | 0.0017 | 0.0026 | 0.0015 | 0.0008 | 0.0017 | 0.0004 | 0.0026 | 0.0009 | 0.0009 | 0.0008 | 0.0004 | 0.0007 | 0.0008 | 0.0025 | 0.0009 | 0.0004 | 0.0004 | 0.0006 |
Peroxisomes | 0.0356 | 0.0361 | 0.0276 | 0.0425 | 0.084 | 0.0214 | 0.0462 | 0.0443 | 0.0279 | 0.0445 | 0.0493 | 0.041 | 0.0305 | 0.0319 | 0.0473 | 0.0397 | 0.0104 | 0.0296 |
Punctate Nuclear | 0.0017 | 0.0027 | 0.0007 | 0.0001 | 0.0017 | 0.0002 | 0.011 | 0.0004 | 0.0004 | 0.0003 | 0.0009 | 0.0007 | 0.0006 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0006 |
Vacuole | 0.0181 | 0.0175 | 0.0278 | 0.009 | 0.0202 | 0.0044 | 0.0295 | 0.0094 | 0.0105 | 0.0113 | 0.0035 | 0.0092 | 0.0099 | 0.0176 | 0.0083 | 0.0051 | 0.0069 | 0.0048 |
Vacuole Periphery | 0.0082 | 0.0061 | 0.0087 | 0.0078 | 0.0039 | 0.0043 | 0.0152 | 0.0033 | 0.0043 | 0.0067 | 0.0026 | 0.0046 | 0.0048 | 0.0053 | 0.0035 | 0.0036 | 0.0041 | 0.003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.3122 | 12.6754 | 16.5312 | 17.9308 | 12.256 | 8.5887 | 16.2143 | 20.1833 | 18.8818 | 17.2013 |
Translational Efficiency | 0.9314 | 0.8622 | 0.7145 | 0.6994 | 0.6693 | 1.2383 | 0.7742 | 0.6072 | 0.6475 | 0.8038 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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