Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.99 0.97 0.95 0.87 0.83 0.75 0.74 0.72 0.82 0.79 0.98 0.98 0.97 0.98 0.96 0.96 0.97 0.93 0.93 0.94 0.93 0.95
Endoplasmic Reticulum 0 0 0 0 0.05 0.12 0.14 0.18 0.18 0.19 0.14 0.17 0 0 0 0.08 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 8 15 11 17 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 1 4 3 2 0 0 1 1 3 1 1 0 0 1 0 0 1 2 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 4 6 8 18 15 10 21 7 8 5 25 17 4 1 4 0 0 0 0 1 1
Cytoplasm 217 97 143 186 333 212 336 295 184 253 153 187 468 852 795 118 46 96 203 94 137 328 605 652
Endoplasmic Reticulum 3 3 1 5 19 29 58 72 45 66 27 40 8 15 12 10 4 4 2 1 3 7 13 16
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 1 0 6 6 12 14 11 0 5 1 1 2 1 1 0 0 0 1 0 0 0
Mitochondria 0 0 0 0 0 0 0 1 0 1 1 1 0 0 1 0 0 0 0 1 0 0 1 1
Nucleus 0 0 0 0 0 1 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 1 5 3 5 1 5 2 3 4 1 1 0 0 2 0 4 9 3
Unique Cell Count 219 100 144 192 349 244 406 391 248 349 187 238 478 869 816 121 48 100 211 102 148 350 653 690
Labelled Cell Count 220 101 148 197 358 259 432 419 270 374 194 251 485 899 832 135 53 104 211 102 148 350 653 690


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 78.8 114.1 88.6 88.9 68.2 51.0 44.3 38.2 39.3 36.2 35.5 37.0 96.1 98.7 90.9 322.8 338.8 324.4 98.6 97.2 105.9
Std Deviation (1e-4) 23.8 30.6 23.9 33.8 14.7 11.4 11.5 9.3 12.9 10.0 8.5 9.7 20.3 21.5 20.7 124.2 141.2 117.0 22.7 24.0 25.3
Intensity Change (Log2) – – – 0.0 -0.38 -0.8 -1.0 -1.21 -1.17 -1.29 -1.32 -1.26 0.12 0.16 0.04 1.87 1.94 1.87 0.16 0.13 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.3 0 0 1.8 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -2.2 -4.2 -5.1 -6.4 -6.4 -6.8 -5.1 -5.7 -1.1 -1.1 -1.4 -1.2 -1.7 -1.8
Endoplasmic Reticulum 0 2.4 4.0 4.5 5.3 5.2 5.4 4.5 4.9 0 0 0 3.1 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 2.5 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 104.3309 136.7416 119.4752 115.4744 87.4253 119.2494 106.1941 153.5483 132.4056 106.2758 126.2308 143.0174 126.0564 159.144 140.0432 121.6026 122.0663 118.3657
Actin 0.0008 0.0003 0.0005 0.0003 0.0014 0.0003 0.0086 0 0.0014 0.0006 0.0009 0.0001 0.0014 0.0001 0.0047 0.0004 0.0005 0.0012
Bud 0.0018 0.0014 0.0013 0.0044 0.0024 0.0003 0.0049 0.0003 0.0009 0.0012 0.0009 0.0001 0.0034 0.0001 0.0009 0.0013 0.0013 0.0021
Bud Neck 0.0019 0.0004 0.0013 0.0004 0.0008 0.0013 0.0008 0.0002 0.0002 0.0004 0.0005 0.0015 0.0004 0.0001 0.0013 0.0004 0.0006 0.0064
Bud Periphery 0.0017 0.0006 0.0011 0.0089 0.0021 0.0002 0.0044 0 0.0004 0.0015 0.0003 0 0.0066 0 0.0011 0.0009 0.0012 0.0011
Bud Site 0.0036 0.0009 0.0024 0.0002 0.0004 0.0001 0.0118 0.0001 0.0003 0.0003 0.0003 0 0.0052 0.0001 0.0006 0.0002 0.0003 0.0007
Cell Periphery 0.0157 0.0101 0.0077 0.0021 0.0025 0.0014 0.0082 0.001 0.0021 0.0106 0.0007 0.0066 0.0091 0.002 0.0019 0.0096 0.0292 0.0034
Cytoplasm 0.7763 0.9463 0.8565 0.8978 0.7119 0.7893 0.7427 0.971 0.8963 0.7255 0.8451 0.9312 0.788 0.9784 0.8787 0.7784 0.768 0.7376
Cytoplasmic Foci 0.011 0.0014 0.015 0.0012 0.029 0.002 0.0187 0.0006 0.0111 0.0199 0.0044 0.0052 0.0046 0.0005 0.0124 0.0114 0.0041 0.0705
Eisosomes 0.0001 0 0.0002 0 0.0002 0 0.0007 0 0.0001 0.0006 0 0 0.0002 0 0.0002 0.0006 0.0003 0.0003
Endoplasmic Reticulum 0.0335 0.008 0.0248 0.0022 0.0031 0.1628 0.0125 0.0121 0.0034 0.0038 0.0064 0.0032 0.0264 0.0015 0.0049 0.0155 0.0049 0.0113
Endosome 0.0102 0.0011 0.0026 0.0025 0.0051 0.0046 0.0175 0.0005 0.0028 0.0285 0.0484 0.0041 0.0059 0.0006 0.0132 0.003 0.0316 0.0144
Golgi 0.0016 0.0002 0.0005 0.0001 0.0004 0.0011 0.0065 0.0001 0.0012 0.004 0.0061 0.0003 0.001 0.0001 0.0076 0.0008 0.0181 0.0009
Lipid Particles 0.0019 0.0009 0.0025 0.0001 0.0028 0.0002 0.0051 0.0001 0.0017 0.0188 0.0008 0.0085 0.0014 0.0001 0.006 0.0022 0.0074 0.0043
Mitochondria 0.0038 0.0009 0.0076 0.0047 0.0067 0.0003 0.0278 0 0.0106 0.0277 0.0062 0.0001 0.0044 0 0.007 0.0167 0.0114 0.0206
None 0.0044 0.0006 0.0027 0.0005 0.0208 0.0004 0.0131 0.0001 0.0046 0.0076 0.0029 0.0006 0.0021 0.0001 0.0051 0.0109 0.0016 0.0323
Nuclear Periphery 0.0138 0.0012 0.004 0.0025 0.0421 0.0069 0.0157 0.0012 0.0026 0.0051 0.0142 0.0048 0.0272 0.0009 0.0096 0.009 0.0022 0.0068
Nucleolus 0.0086 0.0014 0.0056 0.0002 0.0276 0.0002 0.0103 0 0.0095 0.018 0.002 0.0015 0.0014 0.0001 0.0024 0.0066 0.0024 0.021
Nucleus 0.0092 0.0016 0.004 0.002 0.0514 0.0021 0.0081 0.0009 0.0041 0.0046 0.0098 0.0043 0.0209 0.0007 0.0024 0.0055 0.0045 0.0088
Peroxisomes 0.0004 0.0001 0.0007 0.0001 0.0005 0 0.0154 0 0.0055 0.002 0.0002 0 0.0079 0 0.0014 0.0003 0.0014 0.0014
Punctate Nuclear 0.0014 0 0.0002 0 0.0019 0.0001 0.0008 0 0.0002 0.0004 0.0008 0.0004 0.0006 0 0.0008 0.0003 0.0001 0.0033
Vacuole 0.0802 0.0205 0.0503 0.0597 0.0698 0.0227 0.052 0.011 0.0302 0.0917 0.0369 0.0243 0.0618 0.0127 0.0276 0.1016 0.0884 0.0379
Vacuole Periphery 0.018 0.0021 0.0084 0.0102 0.0169 0.0037 0.0143 0.0007 0.0108 0.0272 0.0121 0.0032 0.0202 0.0016 0.0102 0.0244 0.0206 0.0138

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2547.4006 2997.7113 3460.5028 2428.4603 3147.1033 2755.1085 2232.954 2039.4128 2425.5046 2026.0505
Translational Efficiency 1.4694 1.3649 1.3135 2.1157 1.5855 1.2152 1.4787 1.9085 1.43 1.7152

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rps9b

Rps9b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps9b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available