Standard name | |
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Human Ortholog | |
Description | Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 1 |
Bud | 0 | 0 | – | 0 | 0 | 2 | – | 11 | 6 | 11 | 9 | 5 | 0 | 1 | 0 | 0 | 1 | 0 | 5 | 2 | – | 4 | 2 | 6 |
Bud Neck | 1 | 0 | – | 0 | 0 | 0 | – | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | 1 | 1 | 1 |
Bud Site | 0 | 0 | – | 0 | 1 | 8 | – | 9 | 10 | 17 | 12 | 19 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
Cytoplasm | 44 | 16 | – | 26 | 57 | 41 | – | 49 | 38 | 43 | 39 | 33 | 82 | 167 | 222 | 68 | 41 | 39 | 11 | 1 | – | 9 | 8 | 10 |
Endoplasmic Reticulum | 2 | 3 | – | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 11 | 3 | 8 | 0 | 1 | – | 1 | 2 | 3 |
Endosome | 2 | 0 | – | 0 | 9 | 1 | – | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 24 | 20 | 6 | 3 | 7 | 2 | – | 11 | 10 | 9 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 1 | – | 2 | 5 | 11 |
Mitochondria | 108 | 11 | – | 169 | 105 | 292 | – | 382 | 429 | 444 | 353 | 411 | 8 | 9 | 8 | 17 | 10 | 3 | 10 | 4 | – | 8 | 15 | 14 |
Nucleus | 817 | 289 | – | 266 | 291 | 261 | – | 425 | 327 | 390 | 285 | 280 | 457 | 660 | 688 | 67 | 49 | 64 | 811 | 283 | – | 295 | 278 | 278 |
Nuclear Periphery | 1 | 0 | – | 3 | 2 | 2 | – | 1 | 4 | 3 | 4 | 3 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 3 |
Nucleolus | 0 | 0 | – | 3 | 0 | 2 | – | 8 | 6 | 7 | 3 | 3 | 1 | 3 | 4 | 3 | 0 | 0 | 3 | 0 | – | 7 | 19 | 13 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 |
SpindlePole | 4 | 0 | – | 0 | 0 | 2 | – | 1 | 1 | 1 | 2 | 1 | 1 | 1 | 9 | 2 | 0 | 1 | 16 | 0 | – | 8 | 8 | 10 |
Vac/Vac Membrane | 35 | 17 | – | 12 | 42 | 28 | – | 29 | 22 | 23 | 19 | 27 | 23 | 55 | 66 | 55 | 26 | 22 | 96 | 26 | – | 105 | 123 | 177 |
Unique Cell Count | 917 | 318 | 360 | 433 | 448 | 685 | 562 | 657 | 498 | 525 | 558 | 882 | 986 | 212 | 120 | 127 | 972 | 332 | 464 | 486 | 550 | |||
Labelled Cell Count | 1014 | 336 | 479 | 509 | 639 | 917 | 843 | 941 | 726 | 782 | 574 | 904 | 1031 | 244 | 137 | 140 | 972 | 332 | 464 | 486 | 550 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.5 | – | 3.9 | 4.3 | 3.5 | – | 3.8 | 3.2 | 3.5 | 3.4 | 3.3 | 5.2 | 5.5 | 5.3 | 5.7 | 6.5 | 5.9 | 5.4 | 5.6 | 5.6 |
Std Deviation (1e-4) | 0.6 | 1.2 | – | 1.6 | 1.9 | 1.2 | – | 1.4 | 0.8 | 1.1 | 1.2 | 1.1 | 1.2 | 1.3 | 1.2 | 1.7 | 2.7 | 2.1 | 1.5 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2485 | 1.4598 | 1.0773 | 1.2499 | 1.1845 | 1.4007 | 0.5888 | 0.4694 | 0.3044 | 0.5854 | 0.4634 | 0.326 | 2.1642 | 2.0733 | 1.8956 | 1.7648 | 1.23 | 1.7928 |
Actin | 0.0089 | 0 | 0.009 | 0.0143 | 0.0043 | 0.0018 | 0.0203 | 0.0001 | 0.0155 | 0.0067 | 0.0056 | 0.0018 | 0.0333 | 0 | 0.0196 | 0.0055 | 0.0153 | 0.0013 |
Bud | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0006 | 0.0002 | 0.0005 | 0.0008 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0008 | 0.0001 | 0.0011 | 0.0234 | 0.0039 | 0.0009 | 0.0019 | 0.0003 | 0.0012 | 0.001 | 0.001 | 0.0049 | 0.0175 | 0.0002 | 0.0013 | 0.0011 | 0.0012 | 0.0015 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0027 | 0.0001 | 0.0014 | 0.0002 | 0.0015 | 0.0029 | 0.0002 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0004 |
Bud Site | 0.0002 | 0.0001 | 0.0015 | 0.0009 | 0.0017 | 0.0001 | 0.002 | 0.0006 | 0.0028 | 0.0017 | 0.0006 | 0.0002 | 0.0033 | 0.0003 | 0.0007 | 0.0004 | 0.0003 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 | 0 | 0.0003 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0031 | 0.0041 | 0.0006 | 0.0003 | 0.0014 | 0.0006 | 0.0093 | 0.0053 | 0.0018 | 0.0011 | 0.0289 | 0.0034 | 0.013 | 0.0021 | 0.0037 | 0.0042 | 0.0164 | 0.001 |
Cytoplasmic Foci | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0003 | 0.0116 | 0.0007 | 0.0051 | 0.004 | 0.0093 | 0.0005 | 0.0029 | 0 | 0.003 | 0.0005 | 0.0014 | 0.0002 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.001 | 0.0001 | 0.0007 | 0.0002 | 0.0009 | 0.0002 | 0.0031 | 0.0003 | 0.0004 | 0.0009 | 0.0035 | 0.001 | 0.0032 | 0.0001 | 0.0013 | 0.0016 | 0.0027 | 0.0004 |
Endosome | 0.004 | 0.0001 | 0.0015 | 0.0007 | 0.0038 | 0.0018 | 0.0244 | 0.0008 | 0.0074 | 0.0163 | 0.0116 | 0.0033 | 0.0133 | 0.0002 | 0.0048 | 0.0033 | 0.0132 | 0.002 |
Golgi | 0.0008 | 0 | 0.0006 | 0.0008 | 0.0007 | 0.0006 | 0.0055 | 0.0001 | 0.0063 | 0.0113 | 0.0204 | 0.0011 | 0.003 | 0 | 0.0013 | 0.0003 | 0.0044 | 0.0005 |
Lipid Particles | 0.001 | 0 | 0.0003 | 0.0002 | 0.0011 | 0.0011 | 0.0092 | 0.0021 | 0.0131 | 0.0723 | 0.0118 | 0.0016 | 0.0032 | 0 | 0.0046 | 0.0003 | 0.0074 | 0.0006 |
Mitochondria | 0.0011 | 0.0001 | 0.0006 | 0.0003 | 0.0041 | 0.0004 | 0.0305 | 0.0013 | 0.0053 | 0.0412 | 0.0129 | 0.0071 | 0.003 | 0.0002 | 0.0006 | 0.0007 | 0.0031 | 0.001 |
None | 0.0075 | 0.0034 | 0.0004 | 0.0002 | 0.0013 | 0.0002 | 0.0289 | 0.0065 | 0.0017 | 0.0012 | 0.0034 | 0.0026 | 0.0177 | 0.0022 | 0.012 | 0.0106 | 0.0052 | 0.0007 |
Nuclear Periphery | 0.0145 | 0.0036 | 0.0099 | 0.0015 | 0.0186 | 0.007 | 0.0146 | 0.0093 | 0.0098 | 0.0058 | 0.0076 | 0.0083 | 0.0227 | 0.0081 | 0.0145 | 0.0254 | 0.0457 | 0.0071 |
Nucleolus | 0.0111 | 0.0104 | 0.0091 | 0.0107 | 0.0226 | 0.0197 | 0.0103 | 0.0147 | 0.012 | 0.0048 | 0.0118 | 0.0166 | 0.0121 | 0.0125 | 0.0169 | 0.011 | 0.0071 | 0.024 |
Nucleus | 0.9296 | 0.9664 | 0.9513 | 0.9402 | 0.9063 | 0.9475 | 0.8009 | 0.947 | 0.8952 | 0.7902 | 0.8407 | 0.9277 | 0.8158 | 0.9635 | 0.8969 | 0.8721 | 0.8538 | 0.9483 |
Peroxisomes | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0047 | 0.0014 | 0.0065 | 0.014 | 0.0242 | 0.0009 | 0.0021 | 0 | 0.0054 | 0.0001 | 0.0009 | 0.0001 |
Punctate Nuclear | 0.0131 | 0.0112 | 0.0123 | 0.0048 | 0.0207 | 0.0171 | 0.0136 | 0.0081 | 0.0114 | 0.0148 | 0.0045 | 0.0176 | 0.0278 | 0.0101 | 0.0113 | 0.0615 | 0.0174 | 0.0097 |
Vacuole | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.0023 | 0.0002 | 0.0039 | 0.0007 | 0.0015 | 0.0051 | 0.0011 | 0.0006 | 0.0029 | 0.0003 | 0.0013 | 0.0005 | 0.0013 | 0.0005 |
Vacuole Periphery | 0.0007 | 0 | 0.0002 | 0.0001 | 0.0014 | 0.0002 | 0.0028 | 0.0002 | 0.0006 | 0.0031 | 0.0005 | 0.0004 | 0.001 | 0.0001 | 0.0003 | 0.0005 | 0.0021 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.3138 | 18.5608 | 7.8703 | 11.1922 | 7.6915 | 2.9645 | 16.0859 | 24.428 | 13.6957 | 12.1425 |
Translational Efficiency | 0.8457 | 0.9227 | 2.1738 | 1.6209 | 1.7691 | 6.8649 | 1.038 | 0.8909 | 1.6203 | 1.413 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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