Standard name
Human Ortholog
Description Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.05 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0.05 0.06 0.07 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.05 0.07 0.05 0.06 0.05 0.05 0 0.05 0.14 0.2 0.23 0.11 0.12 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.28 0.46 0.07 0.15 0.19 0.2 0.12 0.08 0.09 0.12 0.07 0.15 0.29 0.37 0.47 0.47 0.38 0.42 0.09 0.1 0.05 0.09 0.13 0.12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.05 0 0 0 0.05 0.13 0.08 0.19 0.15 0.32 0.13 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.05 0 0 0.06 0.06 0 0 0 0.06 0 0 0.05 0.07 0.08 0.06 0 0 0 0 0.05 0 0.06 0.06 0.06
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.71 0.65 0.54 0.41 0.41 0.43 0.46 0.42 0.47 0.38 0.48 0.45 0.32 0.23 0.22 0.47 0.47 0.43 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.53 0.4 0.86 0.77 0.8 0.73 0.67 0.69 0.56 0.67 0.49 0.64 0.6 0.53 0.47 0.55 0.54 0.56 0.77 0.76 0.86 0.69 0.61 0.56
Vac/Vac Membrane 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.12 0.13 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 4 2 1 2 3 2 2 2 2 0 2 1 0 5 3 0 0 0 1 0 4
Bud 3 2 3 2 9 4 18 12 18 20 10 7 0 1 1 1 1 2 9 1 1 8 17 25
Bud Neck 0 0 4 2 3 2 6 6 8 5 3 3 3 1 1 0 0 0 3 1 0 2 5 15
Bud Site 3 0 0 6 5 13 19 15 21 18 6 6 0 1 1 1 2 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 5 8 8 25 23 19 14 16 17 8 10 45 125 120 27 35 26 2 1 3 4 8 14
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 3 7 1 0 0 0 1 1 4
Endosome 93 56 12 32 85 68 42 20 29 42 13 28 90 229 247 112 114 101 31 16 10 26 55 67
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 3 2 1 0 0 1 2 4
Mitochondria 34 6 1 4 5 17 45 20 61 54 61 25 0 0 0 13 13 10 1 0 0 2 4 7
Nucleus 18 3 2 13 26 12 15 8 18 12 8 10 21 47 32 7 6 6 9 7 2 18 23 33
Nuclear Periphery 1 0 0 1 0 1 0 2 0 0 0 0 1 0 0 1 0 0 1 1 0 1 0 3
Nucleolus 232 79 88 87 188 149 164 106 149 137 91 84 100 142 118 113 143 105 10 3 2 9 14 26
Peroxisomes 6 1 0 1 10 8 5 1 4 6 2 4 0 0 13 2 4 1 1 0 0 0 0 2
SpindlePole 176 49 139 162 366 254 240 175 177 241 92 121 187 328 248 131 163 136 274 119 172 209 251 327
Vac/Vac Membrane 13 13 5 5 9 2 7 2 4 5 5 5 13 25 32 28 38 20 4 2 2 6 12 18
Unique Cell Count 329 122 162 211 459 347 358 253 317 359 188 188 312 620 531 238 303 243 355 157 202 304 413 581
Labelled Cell Count 585 214 263 327 734 554 582 384 507 559 301 305 461 903 814 440 534 416 355 157 202 304 413 581


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.8 6.7 6.5 6.5 5.3 5.1 5.6 4.8 5.2 4.7 5.4 6.9 7.0 7.1 9.1 9.8 9.3 7.0 7.2 7.6
Std Deviation (1e-4) 1.1 1.0 1.1 1.4 1.6 1.1 1.1 1.1 0.9 1.1 0.8 1.2 1.0 0.9 1.0 1.8 2.3 1.8 1.2 1.3 1.4
Intensity Change (Log2) – – – -0.05 -0.04 -0.34 -0.39 -0.27 -0.48 -0.37 -0.5 -0.32 0.03 0.07 0.08 0.43 0.54 0.47 0.05 0.11 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1.7 1.5 0 1.9 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.0 3.2 0 2.9 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 0.2 0.7 0.2 0.3 0.1 -0.1 -0.3 0.2 3.1 4.6 5.1 2.2 2.3 2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 2.3 3.3 3.5 1.5 0.2 0 1.5 0 2.2 5.4 7.2 9.0 8.4 7.0 7.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.4 4.4 3.3 0 5.0 0 4.5 0 0 0 2.6 0 0
Nucleus 2.4 2.3 0 1.8 0 2.3 0 0 2.1 2.6 3.0 2.5 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.5 -2.9 -2.4 -1.8 -2.5 0 -3.4 0 -1.8 -4.7 -7.8 -7.8 -1.3 -1.5 -2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.2 -1.7 -3.2 -4.5 -3.9 0 -4.4 0 -4.6 -5.8 -7.6 -8.8 -6.4 -6.9 -6.3
Vacuole 0 0 0 0 0 0 0 0 0 0.6 0.6 1.5 3.1 3.4 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9877 1.2203 1.1642 1.0105 0.5937 0.9619 3.1946 2.6688 3.4923 2.7482 2.0176 2.835 2.531 2.1064 1.8859 2.2206 2.42 2.322
Actin 0.0126 0.0004 0.0001 0.0001 0.0001 0 0 0 0 0 0 0 0.0124 0.0001 0.0067 0.0003 0.0019 0.0027
Bud 0.0001 0.0011 0.0003 0.0001 0.0002 0 0 0 0.0012 0 0 0 0.0001 0.0009 0.0001 0 0 0.0001
Bud Neck 0.0014 0.0028 0.0021 0.0039 0.0171 0.0021 0.0002 0.0011 0.0006 0.0014 0.0004 0.0004 0.0015 0.0019 0.0071 0.0005 0.0009 0.0013
Bud Periphery 0.0001 0.0004 0.0002 0 0.0002 0 0 0 0.0001 0 0 0 0.0001 0.0002 0.0001 0 0.0001 0.0001
Bud Site 0.0012 0.0132 0.0018 0.0008 0.0028 0.0002 0.0001 0.0008 0.0011 0.0001 0 0 0.0005 0.0039 0.0033 0.0001 0.0002 0.0003
Cell Periphery 0 0.0001 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.0023 0.0105 0.0008 0.0005 0.0005 0.0015 0.0021 0.0013 0.0011 0.0001 0 0.0002 0.0009 0.0064 0.0006 0.0018 0.0135 0.0092
Cytoplasmic Foci 0.0299 0.0111 0.0058 0.069 0.1353 0.0084 0.0017 0.0033 0.0018 0.0449 0.0091 0.0036 0.0189 0.009 0.0104 0.032 0.111 0.0156
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0001 0.0009 0.0001 0 0 0 0.0001 0 0 0 0 0 0.0006 0.0001 0.0001 0.0001 0.0016 0.0008
Endosome 0.0065 0.0077 0.001 0.013 0.01 0.0006 0.0004 0.0007 0.0001 0.0006 0.0001 0.0001 0.003 0.0014 0.0013 0.0008 0.0117 0.0027
Golgi 0.0008 0.0008 0.0004 0.0034 0.0036 0.0003 0.0001 0.0001 0.0001 0.0004 0.0002 0.0001 0.0012 0.0004 0.0009 0.0004 0.0034 0.0004
Lipid Particles 0.008 0.0041 0.0036 0.0232 0.0156 0.0024 0.0002 0.0001 0.0002 0.001 0.0024 0.0004 0.0019 0.0013 0.0029 0.0007 0.0168 0.0009
Mitochondria 0.0001 0.0002 0.0003 0.0001 0.0001 0 0 0 0.0001 0 0 0 0.0003 0.0004 0.0097 0.0001 0.0001 0.0003
None 0.0005 0.0022 0.0004 0.0002 0.0002 0.0009 0.0001 0.0003 0.0011 0.0001 0 0 0.0005 0.0006 0.0006 0.0009 0.0168 0.0013
Nuclear Periphery 0.0063 0.0052 0.0011 0.0017 0.0003 0.0009 0.0005 0.0022 0.0002 0.0003 0.0001 0.0002 0.0097 0.0022 0.0007 0.0025 0.0027 0.0025
Nucleolus 0.0385 0.0569 0.061 0.0252 0.071 0.037 0.0074 0.044 0.034 0.0014 0.0211 0.0092 0.018 0.037 0.024 0.0029 0.0021 0.0125
Nucleus 0.0701 0.1804 0.0783 0.1647 0.0097 0.0225 0.026 0.0804 0.031 0.001 0.0017 0.0094 0.0171 0.1003 0.0391 0.0253 0.0013 0.0363
Peroxisomes 0.0009 0.0008 0.0013 0.0064 0.0097 0.0005 0 0.0001 0.0001 0.0026 0.0005 0.0003 0.0008 0.0008 0.0012 0.0027 0.0098 0.0012
Punctate Nuclear 0.8185 0.6977 0.8406 0.6857 0.7223 0.9225 0.9611 0.8653 0.9271 0.9461 0.9643 0.976 0.9119 0.8324 0.8907 0.9287 0.8056 0.9113
Vacuole 0.0016 0.003 0.0004 0.0017 0.0013 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0006 0.0002 0.0001 0.0004 0.0005
Vacuole Periphery 0.0002 0.0003 0.0001 0.0001 0.0001 0 0 0 0 0 0 0 0.0002 0.0001 0.0002 0 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.7902 12.14 6.0056 11.7941 8.0701 3.3932 13.5534 13.4625 9.0094 11.0707
Translational Efficiency 1.5407 0.8259 1.6164 0.9952 1.2686 3.8424 0.9856 1.0657 1.5312 1.2318

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Essential kinetochore protein associated with microtubules and SPBs; component of the kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p); involved in spindle checkpoint maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-U and fission yeast Mis17; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages spindle pole (72%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ame1

Ame1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ame1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available