Standard name
Human Ortholog
Description RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0.2 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 0.98 0.89 0.87 0.86 0.76 0.68 0.67 0.61 1.0 1.0 0.99 0.98 0.94 0.95 0.96 0.91 0.94 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.07 0.13 0.12 0.27 0.21 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.09 0.08 0.05 0.18 0.09 0.21 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 1 0 2 0 1 4 1 2 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 1 2 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 6 6 7 7 6 7 3 12 13 13 0 7 4 10 0 0 0 0 0 0 0
Cytoplasm 17 209 162 164 192 229 229 123 136 123 127 186 251 344 50 17 49 199 150 76 194 207
Endoplasmic Reticulum 0 1 0 0 4 7 4 15 6 8 4 0 1 0 0 1 0 1 2 1 1 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 3 0
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 0
Mitochondria 0 0 0 0 0 6 19 21 24 49 44 0 0 0 0 0 0 0 1 0 1 1
Nucleus 0 1 1 0 2 1 1 1 2 1 3 1 0 1 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 1 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 5 3 20 24 20 8 35 16 44 0 0 3 0 1 0 3 1 0 0 0
Unique Cell Count 17 212 166 167 216 264 265 161 200 183 208 186 252 347 51 18 52 209 165 82 208 220
Labelled Cell Count 17 220 174 175 227 277 282 172 221 212 241 187 259 353 61 19 52 209 165 82 208 220


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.3 11.2 11.1 10.3 11.9 10.3 10.3 8.9 10.0 8.5 9.2 10.6 11.1 11.0 14.6 12.4 9.2 10.5 10.9
Std Deviation (1e-4) 1.2 1.7 2.0 2.2 2.4 1.8 1.8 2.0 2.3 2.1 2.2 1.5 1.4 1.5 1.9 1.9 1.8 1.6 1.7
Intensity Change (Log2) -0.1 0.1 -0.11 -0.11 -0.31 -0.15 -0.39 -0.27 -0.06 0.01 -0.02 0.39 0.17 -0.26 -0.08 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.3 -0.2 0 -0.6 0 1.1 1.4 1.2 0 -0.5 0 0 0
Cytoplasm 0.4 -3.2 -3.8 -3.9 -5.7 -7.2 -7.3 -8.4 2.1 1.9 1.4 0.2 -0.8
Endoplasmic Reticulum 0 0 0 0 4.0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.5 4.8 4.6 7.2 6.3 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.4 2.4 2.0 0.9 4.4 2.2 5.2 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5299 5.3963 5.3157 5.2003 3.4751 5.2344 6.1797 7.6477 6.9097 6.2253 5.9414 7.2387 7.6508 8.4193 7.6815 7.7508 7.2862 7.8984
Actin 0.0057 0.0084 0.0071 0.0004 0.0036 0.0138 0.0323 0.0007 0.003 0.0354 0.0473 0.0019 0.0212 0.0006 0.002 0.0002 0.0006 0.0008
Bud 0.0002 0.0002 0.0023 0.0001 0.0007 0.0004 0.0034 0.0054 0.0034 0.0043 0.0004 0.0015 0.0004 0.0011 0.0005 0.0001 0.0001 0.0005
Bud Neck 0.007 0.0003 0.0003 0.0003 0.0009 0.0011 0.0016 0.0001 0.0003 0.0015 0.0011 0.0012 0.0022 0.0002 0.0003 0.0003 0.0003 0.0009
Bud Periphery 0.0001 0.0001 0.001 0 0.0003 0.0004 0.0018 0.0017 0.0013 0.0031 0.0006 0.0005 0.0005 0.0004 0.0006 0 0 0.0003
Bud Site 0.0037 0.0012 0.001 0 0.0114 0.0007 0.0117 0.005 0.0013 0.02 0.0008 0.0001 0.001 0.0008 0.0003 0 0.0002 0.0001
Cell Periphery 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0002 0.0003 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.85 0.9186 0.9054 0.9462 0.847 0.9275 0.7858 0.9708 0.9124 0.8048 0.825 0.9638 0.8951 0.9872 0.965 0.9865 0.9288 0.9811
Cytoplasmic Foci 0.0312 0.0044 0.0176 0.0026 0.0457 0.0069 0.0382 0.0027 0.0143 0.0185 0.029 0.0106 0.0129 0.0022 0.0043 0.002 0.0116 0.0032
Eisosomes 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0 0 0.0001 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0139 0.0033 0.0027 0.0364 0.0039 0.0053 0.0159 0.0019 0.0086 0.0092 0.0016 0.0053 0.0108 0.0013 0.005 0.0028 0.0033 0.0022
Endosome 0.0168 0.007 0.0096 0.003 0.0509 0.009 0.0259 0.0011 0.0123 0.0202 0.0076 0.0047 0.0209 0.0009 0.0075 0.002 0.0234 0.0035
Golgi 0.0097 0.0101 0.0134 0.0001 0.021 0.0039 0.0087 0.0001 0.0109 0.0045 0.02 0.0019 0.003 0.0001 0.0013 0.0001 0.0013 0.0004
Lipid Particles 0.0066 0.0009 0.0026 0 0.0015 0.0051 0.007 0 0.0027 0.0179 0.0095 0 0.0012 0 0.0012 0 0.0067 0.0005
Mitochondria 0.001 0.0017 0.01 0.0001 0.0028 0.0014 0.0086 0.0008 0.0013 0.0193 0.0127 0.0012 0.0031 0.0001 0.0008 0.0001 0.0005 0.0002
None 0.0292 0.0363 0.0124 0.0018 0.0014 0.0013 0.0158 0.006 0.0143 0.0077 0.0036 0.0021 0.0075 0.0018 0.0029 0.0014 0.0034 0.0016
Nuclear Periphery 0.0032 0.0026 0.0009 0.0043 0.0009 0.0065 0.0168 0.0008 0.0007 0.0035 0.0009 0.0009 0.0088 0.0005 0.0011 0.0008 0.005 0.0008
Nucleolus 0.0007 0.0001 0 0 0.0001 0.0006 0.0005 0.0001 0.0001 0.0005 0.0001 0 0.0004 0 0.0001 0 0.0002 0
Nucleus 0.0019 0.002 0.001 0.0034 0.0012 0.0027 0.0048 0.0016 0.0014 0.005 0.0008 0.0014 0.0036 0.0021 0.0019 0.0025 0.0048 0.0024
Peroxisomes 0.0076 0.0002 0.01 0 0.0037 0.0011 0.0129 0.0001 0.0094 0.0177 0.0083 0.0016 0.0023 0 0.0009 0 0.0012 0.0003
Punctate Nuclear 0.0093 0.0006 0.0014 0.0001 0.0008 0.0103 0.0046 0.0005 0.0007 0.0014 0.0295 0.0004 0.0022 0.0002 0.0005 0.0001 0.0067 0.0005
Vacuole 0.0013 0.0013 0.0008 0.0007 0.0016 0.0015 0.0022 0.0005 0.0013 0.0037 0.0004 0.0005 0.0017 0.0003 0.003 0.001 0.0012 0.0004
Vacuole Periphery 0.0005 0.0003 0.0002 0.0002 0.0006 0.0005 0.0013 0.0001 0.0002 0.0012 0.0003 0.0002 0.0009 0.0001 0.0007 0.0002 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.6104 35.1754 26.1016 35.9651 28.6973 40.4194 40.7525 42.508 38.8957 36.6677
Translational Efficiency 1.3108 1.3691 1.607 1.2707 1.4354 1.3969 1.1792 1.3567 1.4003 1.3031

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase
Localization
Cell Percentages cytoplasm (54%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ngr1

Ngr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ngr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available