Standard name | |
---|---|
Human Ortholog | |
Description | Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 0 |
Bud | 1 | 0 | 5 | 2 | 1 | 1 | 2 | 3 | 5 | 4 | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 1 | 3 | 5 | 12 | 3 | 6 | 3 | 7 | 1 | 4 | 5 | 3 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 199 | 232 | 243 | 131 | 233 | 283 | 293 | 344 | 204 | 228 | 128 | 144 | 261 | 364 | 442 | 468 | 267 | 174 | 192 | 227 | 241 | 163 | 274 | 199 |
Endoplasmic Reticulum | 3 | 1 | 1 | 0 | 0 | 4 | 3 | 6 | 7 | 10 | 3 | 8 | 0 | 2 | 1 | 12 | 8 | 5 | 1 | 0 | 1 | 1 | 1 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 1 | 0 | 1 | 1 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 |
Mitochondria | 8 | 3 | 4 | 7 | 12 | 32 | 45 | 55 | 46 | 72 | 37 | 41 | 0 | 1 | 0 | 8 | 2 | 2 | 1 | 1 | 1 | 2 | 2 | 2 |
Nucleus | 4 | 2 | 4 | 0 | 0 | 2 | 4 | 1 | 4 | 4 | 3 | 7 | 1 | 3 | 0 | 5 | 3 | 2 | 0 | 1 | 1 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 2 | 2 | 6 | 8 | 35 | 81 | 22 | 33 | 22 | 17 | 0 | 1 | 1 | 5 | 7 | 2 | 1 | 1 | 4 | 3 | 3 | 5 |
Unique Cell Count | 209 | 238 | 250 | 134 | 245 | 315 | 369 | 476 | 262 | 321 | 174 | 207 | 262 | 369 | 444 | 481 | 274 | 179 | 206 | 245 | 257 | 177 | 297 | 229 |
Labelled Cell Count | 217 | 243 | 260 | 142 | 254 | 333 | 391 | 504 | 293 | 362 | 197 | 230 | 263 | 375 | 449 | 502 | 288 | 189 | 206 | 245 | 257 | 177 | 297 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.6 | 7.3 | 6.0 | 6.2 | 8.3 | 8.3 | 8.1 | 8.9 | 8.9 | 8.5 | 8.8 | 9.1 | 9.9 | 12.5 | 15.3 | 13.5 | 15.4 | 17.3 | 7.6 | 7.7 | 9.9 |
Std Deviation (1e-4) | 1.1 | 1.1 | 1.1 | 1.0 | 1.4 | 1.9 | 1.9 | 2.0 | 2.3 | 2.3 | 2.6 | 2.8 | 2.4 | 3.5 | 3.8 | 3.2 | 3.4 | 3.8 | 2.3 | 1.7 | 2.1 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.46 | 0.45 | 0.43 | 0.56 | 0.55 | 0.49 | 0.55 | 0.58 | 0.71 | 1.05 | 1.34 | 1.16 | 1.35 | 1.51 | 0.33 | 0.36 | 0.71 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | -1.2 | -3.4 | -6.4 | -8.1 | -6.6 | -8.2 | -7.2 | -8.2 | 2.2 | 1.3 | 2.6 | 0.1 | 0.2 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 2.1 | 4.1 | 4.8 | 4.7 | 6.1 | 7.3 | 6.7 | 6.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 4.5 | 6.5 | 4.1 | 4.7 | 5.2 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7479 | 6.9569 | 6.4816 | 6.0715 | 7.1281 | 7.1675 | 4.8298 | 5.2817 | 4.7073 | 5.269 | 5.2106 | 4.6871 | 5.9191 | 7.0232 | 6.3048 | 5.5231 | 6.4876 | 6.566 |
Actin | 0.001 | 0.0394 | 0.0033 | 0.0031 | 0.001 | 0.0004 | 0.0175 | 0.0004 | 0.0038 | 0.0009 | 0.0002 | 0.0341 | 0.0774 | 0.0156 | 0.0284 | 0.0083 | 0.0008 | 0.0132 |
Bud | 0.0001 | 0.0006 | 0.0013 | 0.001 | 0.0003 | 0.0001 | 0.0003 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0013 | 0.0002 | 0.0001 | 0.0001 |
Bud Neck | 0.0002 | 0.0005 | 0.0004 | 0.0019 | 0.0012 | 0.001 | 0.0024 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0009 | 0.0022 | 0.0001 | 0.0007 | 0.0003 | 0.0006 | 0.0008 |
Bud Periphery | 0 | 0.0004 | 0.0005 | 0.0008 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0.0008 | 0 | 0.0007 | 0.0001 | 0 | 0.0001 |
Bud Site | 0.0005 | 0.0059 | 0.0016 | 0.0353 | 0.0003 | 0.0001 | 0.0007 | 0.0017 | 0.0017 | 0.0001 | 0 | 0.0002 | 0.0022 | 0.0005 | 0.0126 | 0.0004 | 0 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0 | 0 | 0.0008 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0.0007 | 0 | 0.0003 | 0.0001 | 0 | 0 |
Cytoplasm | 0.8669 | 0.9268 | 0.9135 | 0.8936 | 0.8853 | 0.9838 | 0.7886 | 0.9049 | 0.9133 | 0.9477 | 0.9922 | 0.8453 | 0.7487 | 0.9699 | 0.9165 | 0.8749 | 0.9808 | 0.9431 |
Cytoplasmic Foci | 0.0487 | 0.0046 | 0.0029 | 0.0128 | 0.0024 | 0.0016 | 0.0041 | 0.0022 | 0.0098 | 0.0038 | 0.0014 | 0.0097 | 0.038 | 0.0014 | 0.014 | 0.0219 | 0.0021 | 0.0061 |
Eisosomes | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0.0019 | 0 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0063 | 0.0014 | 0.0037 | 0.003 | 0.0013 | 0.0014 | 0.0189 | 0.0007 | 0.0064 | 0.0023 | 0.0009 | 0.0013 | 0.0072 | 0.0007 | 0.002 | 0.0035 | 0.0031 | 0.0013 |
Endosome | 0.0028 | 0.0011 | 0.0052 | 0.0065 | 0.0007 | 0.0006 | 0.0183 | 0.0017 | 0.0198 | 0.0027 | 0.0004 | 0.0047 | 0.0292 | 0.0005 | 0.0016 | 0.0098 | 0.0011 | 0.0038 |
Golgi | 0.001 | 0.0028 | 0.0014 | 0.0034 | 0.0001 | 0 | 0.0056 | 0.0003 | 0.0042 | 0.0001 | 0 | 0.0084 | 0.0191 | 0.002 | 0.0018 | 0.0389 | 0.0001 | 0.0062 |
Lipid Particles | 0.0005 | 0.0012 | 0.0136 | 0.0068 | 0 | 0 | 0.0032 | 0 | 0.0055 | 0.0001 | 0 | 0.0047 | 0.0173 | 0 | 0.0012 | 0.0023 | 0 | 0.0041 |
Mitochondria | 0.0003 | 0.0017 | 0.0022 | 0.0043 | 0.0002 | 0.0001 | 0.0014 | 0.0002 | 0.0029 | 0.0001 | 0 | 0.0089 | 0.0112 | 0.0001 | 0.0008 | 0.0026 | 0.0001 | 0.0029 |
None | 0.0422 | 0.004 | 0.0295 | 0.0073 | 0.0957 | 0.0035 | 0.0331 | 0.0843 | 0.0171 | 0.0355 | 0.0014 | 0.0757 | 0.0126 | 0.0062 | 0.0057 | 0.0069 | 0.0032 | 0.0042 |
Nuclear Periphery | 0.0013 | 0.0007 | 0.0054 | 0.0013 | 0.001 | 0.0008 | 0.0392 | 0.0003 | 0.0017 | 0.0012 | 0.0004 | 0.0003 | 0.0021 | 0.0003 | 0.0006 | 0.0009 | 0.0014 | 0.0009 |
Nucleolus | 0.0001 | 0 | 0.0012 | 0.0012 | 0 | 0 | 0.0005 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Nucleus | 0.0042 | 0.0023 | 0.0065 | 0.0046 | 0.0094 | 0.0059 | 0.0417 | 0.001 | 0.0024 | 0.0027 | 0.0021 | 0.001 | 0.0016 | 0.002 | 0.0019 | 0.0045 | 0.0058 | 0.0023 |
Peroxisomes | 0.0216 | 0.0052 | 0.004 | 0.0085 | 0.0001 | 0 | 0.0003 | 0 | 0.0006 | 0.0001 | 0 | 0.0034 | 0.0216 | 0.0001 | 0.0087 | 0.0228 | 0 | 0.0096 |
Punctate Nuclear | 0.0015 | 0.0003 | 0.0013 | 0.001 | 0.0004 | 0.0002 | 0.002 | 0.0003 | 0.0005 | 0.0012 | 0.0001 | 0.0002 | 0.0048 | 0.0002 | 0.0006 | 0.0007 | 0.0003 | 0.0005 |
Vacuole | 0.0006 | 0.0006 | 0.0015 | 0.0026 | 0.0002 | 0.0003 | 0.0136 | 0.0006 | 0.0075 | 0.0011 | 0.0002 | 0.0005 | 0.0012 | 0.0003 | 0.0005 | 0.0006 | 0.0004 | 0.0002 |
Vacuole Periphery | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0056 | 0.0002 | 0.001 | 0.0001 | 0 | 0.0002 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.4148 | 9.9634 | 9.7037 | 20.8088 | 7.2253 | 30.6332 | 27.0738 | 32.5089 | 33.717 | 21.1096 |
Translational Efficiency | 1.1084 | 1.4465 | 1.4658 | 1.1525 | 2.2796 | 1.3001 | 1.1644 | 1.4655 | 1.4846 | 1.3398 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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