ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 1 | 0 | 0 | 1 | 0 | – | 2 | 2 | 1 | 1 | 0 | 0 | 0 | – | 2 | 0 | 0 | 2 | 1 | 3 | 5 | 7 |
Bud | 1 | 3 | 0 | 1 | 1 | 3 | 3 | – | 6 | 5 | 4 | 3 | 4 | 1 | 2 | – | 1 | 0 | 0 | 2 | 1 | 2 | 4 | 8 |
Bud Neck | 0 | 0 | 6 | 0 | 1 | 1 | 3 | – | 1 | 3 | 0 | 2 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 3 | 4 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 9 | 20 | 15 | 13 | 4 | 9 | 6 | – | 7 | 6 | 8 | 6 | 18 | 9 | 20 | – | 44 | 23 | 0 | 0 | 1 | 3 | 2 | 2 |
Cytoplasm | 257 | 478 | 332 | 250 | 336 | 348 | 376 | – | 290 | 287 | 207 | 188 | 267 | 272 | 336 | – | 461 | 338 | 242 | 455 | 301 | 201 | 195 | 190 |
Endoplasmic Reticulum | 2 | 6 | 2 | 0 | 0 | 0 | 0 | – | 1 | 2 | 0 | 3 | 0 | 3 | 0 | – | 14 | 13 | 3 | 6 | 4 | 6 | 13 | 12 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 4 | 2 | 4 | 2 | 0 | 1 | 2 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 1 |
Mitochondria | 1 | 3 | 0 | 2 | 9 | 32 | 32 | – | 66 | 76 | 80 | 51 | 1 | 0 | 1 | – | 7 | 4 | 0 | 2 | 9 | 16 | 13 | 8 |
Nucleus | 0 | 1 | 1 | 1 | 1 | 3 | 0 | – | 3 | 3 | 2 | 0 | 0 | 0 | 0 | – | 1 | 4 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 2 | 4 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 1 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Vac/Vac Membrane | 0 | 2 | 0 | 0 | 2 | 0 | 0 | – | 2 | 0 | 1 | 0 | 0 | 0 | 0 | – | 12 | 3 | 0 | 3 | 1 | 13 | 5 | 6 |
Unique Cell Count | 260 | 487 | 335 | 251 | 340 | 363 | 392 | 335 | 339 | 257 | 225 | 269 | 276 | 336 | 475 | 350 | 261 | 482 | 330 | 260 | 256 | 252 | ||
Labelled Cell Count | 270 | 515 | 358 | 268 | 356 | 397 | 421 | 381 | 390 | 305 | 254 | 290 | 286 | 362 | 546 | 389 | 261 | 482 | 330 | 260 | 256 | 252 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 5.8 | 5.8 | 5.2 | 5.8 | 4.7 | 4.9 | – | 4.5 | 4.2 | 4.1 | 4.2 | 5.5 | 5.8 | 5.7 | – | 8.3 | 7.4 | 5.7 | 6.5 | 7.1 |
Std Deviation (1e-4) | 1.0 | 0.8 | 1.0 | 0.9 | 1.3 | 1.0 | 1.2 | – | 1.2 | 1.0 | 1.0 | 0.7 | 1.1 | 1.1 | 0.9 | – | 1.5 | 1.4 | 1.4 | 1.9 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.0 | -0.32 | -0.27 | – | -0.36 | -0.48 | -0.52 | -0.46 | -0.09 | -0.02 | -0.04 | – | 0.51 | 0.34 | -0.02 | 0.14 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0.4 | -2.6 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.2 | -0.8 | 0.9 | – | 2.6 | 1.2 |
Cytoplasm | 0.7 | -0.4 | -2.7 | -2.7 | – | -6.3 | -6.8 | -7.8 | -7.0 | 0.2 | -0.6 | 1.7 | – | -2.0 | -2.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2.4 | 2.8 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5601 | 3.3473 | 2.8712 | 2.8589 | 2.8962 | 3.0429 | 1.3541 | 1.8625 | 1.5697 | 1.4104 | 1.4484 | 1.5856 | 1.2138 | 1.5305 | 1.3585 | 1.1632 | 1.3967 | 1.3911 |
Actin | 0.0153 | 0.0006 | 0.0042 | 0.0014 | 0.0003 | 0.0118 | 0.022 | 0.0034 | 0.0088 | 0.0174 | 0.0608 | 0.0061 | 0.0398 | 0.0047 | 0.011 | 0.003 | 0.0134 | 0.008 |
Bud | 0.0016 | 0.0045 | 0.0091 | 0.0006 | 0.0001 | 0.0002 | 0.0021 | 0.0063 | 0.0041 | 0.0036 | 0.0117 | 0.0018 | 0.0201 | 0.0505 | 0.0435 | 0.0442 | 0.0367 | 0.0347 |
Bud Neck | 0.0003 | 0.0007 | 0.0056 | 0.0015 | 0.0001 | 0.0017 | 0.0109 | 0.0005 | 0.0033 | 0.0009 | 0.01 | 0.0018 | 0.0029 | 0.0012 | 0.0014 | 0.0009 | 0.007 | 0.0052 |
Bud Periphery | 0.0015 | 0.005 | 0.0016 | 0.0008 | 0.0005 | 0.0006 | 0.003 | 0.0061 | 0.006 | 0.0192 | 0.0232 | 0.0064 | 0.0209 | 0.0196 | 0.0333 | 0.0209 | 0.0259 | 0.0334 |
Bud Site | 0.0018 | 0.0114 | 0.0132 | 0.0366 | 0.0001 | 0.0002 | 0.023 | 0.0222 | 0.0233 | 0.0009 | 0.0137 | 0.0003 | 0.025 | 0.1129 | 0.054 | 0.0056 | 0.017 | 0.0039 |
Cell Periphery | 0.002 | 0.0025 | 0.007 | 0.0019 | 0.0005 | 0.003 | 0.0013 | 0.0007 | 0.0006 | 0.0004 | 0.0007 | 0.0003 | 0.0018 | 0.0031 | 0.0022 | 0.0008 | 0.002 | 0.0011 |
Cytoplasm | 0.4289 | 0.6305 | 0.5248 | 0.6066 | 0.3855 | 0.6575 | 0.5468 | 0.8266 | 0.7742 | 0.7238 | 0.4021 | 0.8201 | 0.3528 | 0.5375 | 0.5026 | 0.5239 | 0.4832 | 0.5224 |
Cytoplasmic Foci | 0.0127 | 0.0042 | 0.0048 | 0.0113 | 0.0021 | 0.0038 | 0.0361 | 0.0082 | 0.0126 | 0.0183 | 0.0836 | 0.0162 | 0.0418 | 0.0113 | 0.0242 | 0.01 | 0.0237 | 0.0128 |
Eisosomes | 0.0007 | 0.0003 | 0.0007 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0066 | 0.0021 | 0.0025 | 0.0022 | 0.002 | 0.0019 | 0.0134 | 0.0024 | 0.0037 | 0.0031 | 0.0041 | 0.0044 | 0.0059 | 0.0016 | 0.0031 | 0.0026 | 0.0088 | 0.0022 |
Endosome | 0.0156 | 0.0009 | 0.0025 | 0.0011 | 0.0004 | 0.0008 | 0.0463 | 0.0023 | 0.007 | 0.0167 | 0.0896 | 0.0074 | 0.0189 | 0.0015 | 0.0101 | 0.0037 | 0.0252 | 0.0026 |
Golgi | 0.003 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0108 | 0.0004 | 0.0061 | 0.0049 | 0.0484 | 0.0011 | 0.0042 | 0.0005 | 0.0012 | 0.0003 | 0.0102 | 0.0003 |
Lipid Particles | 0.0067 | 0.0002 | 0.0006 | 0.0004 | 0.0002 | 0.0009 | 0.0151 | 0.0002 | 0.0049 | 0.0059 | 0.0135 | 0.0027 | 0.0043 | 0.0004 | 0.0018 | 0.0004 | 0.0082 | 0.0004 |
Mitochondria | 0.0068 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0004 | 0.0061 | 0.0004 | 0.0051 | 0.0018 | 0.0148 | 0.0007 | 0.002 | 0.0017 | 0.0035 | 0.0017 | 0.0109 | 0.001 |
None | 0.4696 | 0.3319 | 0.4153 | 0.3304 | 0.6068 | 0.3132 | 0.2224 | 0.1156 | 0.1274 | 0.171 | 0.1867 | 0.1197 | 0.4342 | 0.2336 | 0.274 | 0.3641 | 0.2495 | 0.3538 |
Nuclear Periphery | 0.0046 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0003 | 0.0131 | 0.0003 | 0.0012 | 0.0019 | 0.0035 | 0.0014 | 0.0058 | 0.0012 | 0.0029 | 0.0032 | 0.0116 | 0.0022 |
Nucleolus | 0.003 | 0.0002 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0002 | 0.0009 | 0.0001 | 0.0008 | 0.0017 | 0.0019 | 0.001 | 0.0067 | 0.0006 |
Nucleus | 0.0018 | 0.0008 | 0.0029 | 0.0006 | 0.0002 | 0.0003 | 0.0043 | 0.001 | 0.0028 | 0.0014 | 0.0021 | 0.0012 | 0.003 | 0.007 | 0.0067 | 0.0059 | 0.0173 | 0.0073 |
Peroxisomes | 0.0115 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.001 | 0.0092 | 0.0006 | 0.0049 | 0.002 | 0.0126 | 0.0052 | 0.0037 | 0.0016 | 0.0068 | 0.0003 | 0.003 | 0.0005 |
Punctate Nuclear | 0.003 | 0.0019 | 0.0007 | 0.0018 | 0.0001 | 0.0003 | 0.0021 | 0.0003 | 0.0009 | 0.002 | 0.0072 | 0.001 | 0.0075 | 0.0048 | 0.0103 | 0.0036 | 0.0181 | 0.0044 |
Vacuole | 0.002 | 0.0013 | 0.0023 | 0.0012 | 0.0003 | 0.0009 | 0.0084 | 0.0021 | 0.0022 | 0.004 | 0.0084 | 0.0015 | 0.0035 | 0.0031 | 0.0045 | 0.0033 | 0.0172 | 0.0026 |
Vacuole Periphery | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0023 | 0.0002 | 0.0004 | 0.0006 | 0.0024 | 0.0006 | 0.0007 | 0.0003 | 0.0007 | 0.0006 | 0.0042 | 0.0004 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.1643 | 6.2036 | 7.0404 | 10.3605 | 5.5573 | 12.2397 | 8.3941 | 12.327 | 11.8292 | 11.0901 |
Translational Efficiency | 0.6332 | 0.7272 | 0.7024 | 0.643 | 0.8904 | 0.5577 | 0.6631 | 0.5325 | 0.571 | 0.5822 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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