Standard name
Human Ortholog
Description Non-proteolytic ATPase of the AAA family; stimulates incorporation of the pyridoxal phosphate cofactor into Hem1p (5-aminolevulinic acid synthase); localized to the mitochondrial matrix; ortholog of vertebrate CLPX, which promotes erythropoiesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0.73 0.7 0.66 0.57 0.59 0.53 0.44 0.4 0.41 0.49 0.93 0.84 0.9 0.11 0.05 0.13 0 0 0.53 0.75 0.75 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.08 0.06 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.22 0.44 0.31 0.07 0.08 0 0 0 0
Mitochondria 0.93 0.81 0.21 0.3 0.33 0.57 0.49 0.49 0.66 0.69 0.74 0.66 0.05 0.13 0.07 0.12 0.14 0.09 0.72 0.66 0.25 0.1 0.11 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Peroxisomes 0.05 0.21 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0.29 0.17 0.19 0.07 0.08 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.14 0.11 0 0 0 0 0 0
Vac/Vac Membrane 0 0.1 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.15 0.13 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 5 0 0 0 0 0 0 0 0 2 0 0 1 1 5 0 3 19 21 7 1 2 4
Bud 17 4 3 3 7 6 6 11 13 12 10 12 0 0 0 14 4 6 0 2 12 1 6 2
Bud Neck 0 0 0 0 1 0 0 3 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud Site 3 1 0 0 1 1 2 3 1 4 1 1 0 0 0 1 0 2 – – – – – –
Cell Periphery 2 13 3 1 0 1 1 2 5 1 2 4 1 1 1 32 35 13 0 0 0 0 0 0
Cytoplasm 4 3 249 149 238 208 253 301 182 209 144 234 238 140 235 48 21 24 0 1 187 80 174 105
Endoplasmic Reticulum 0 1 1 0 0 0 0 2 1 0 0 1 1 0 0 3 12 7 0 1 2 0 0 1
Endosome 6 14 4 2 11 4 6 13 0 3 2 0 0 2 5 41 25 15 35 12 7 0 4 4
Golgi 1 5 1 0 0 0 0 0 0 0 1 0 5 3 0 100 190 59 43 32 5 4 3 4
Mitochondria 573 282 73 64 118 206 208 277 271 360 261 313 14 21 19 56 60 17 455 253 88 10 25 16
Nucleus 2 0 0 1 0 1 5 8 5 3 4 4 2 0 0 8 3 2 0 0 0 0 0 0
Nuclear Periphery 15 12 3 2 7 16 15 22 18 19 8 9 1 1 2 9 4 5 3 2 1 0 0 0
Nucleolus 57 12 6 0 8 13 14 20 17 18 13 11 0 2 1 14 11 10 1 2 0 0 0 0
Peroxisomes 31 73 30 13 12 1 4 5 1 3 1 3 0 2 8 133 71 36 46 30 19 2 4 6
SpindlePole 9 4 5 4 7 2 3 4 3 7 6 3 0 0 4 48 62 20 6 4 9 0 2 1
Vac/Vac Membrane 27 34 1 2 5 6 7 13 18 25 11 10 1 1 1 68 57 17 7 8 4 2 2 4
Unique Cell Count 616 348 343 214 362 363 427 565 412 519 352 474 256 167 262 456 430 188 628 381 358 107 233 158
Labelled Cell Count 749 463 379 241 415 465 524 684 535 665 466 605 263 174 277 580 555 236 628 381 358 107 233 158


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 11.9 5.3 5.5 5.3 5.2 4.6 5.2 4.8 4.9 4.8 4.4 5.5 5.9 5.9 10.1 11.4 11.0 5.0 5.0 6.3
Std Deviation (1e-4) 1.8 2.8 2.7 3.1 3.1 3.1 2.7 2.9 2.8 2.8 2.6 2.4 2.6 2.9 2.9 2.6 2.9 3.0 2.7 2.3 2.6
Intensity Change (Log2) – – – 0.05 0.01 -0.04 -0.21 -0.04 -0.14 -0.11 -0.16 -0.28 0.04 0.16 0.15 0.92 1.1 1.05 -0.08 -0.08 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1.3 0 1.6 0 0 0 0 0 2.1 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 4.2 4.6 3.9
Cytoplasm -0.8 -2.0 -4.3 -3.9 -5.8 -7.9 -9.3 -8.4 -6.7 6.3 2.8 5.2 -18.0 -19.6 -13.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2.7 0
Endosome 0 1.7 0 0 1.2 0 0 0 0 0 0 0 4.7 3.4 4.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 9.1 14.1 10.8
Mitochondria 2.3 3.4 9.6 0 8.3 0 13.8 0 0 -5.4 -2.4 -4.8 -3.4 -2.7 -3.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 2.9 0 2.7 0 2.5 0 0 0 0 0 1.3 0 0
Nucleolus 0 0.4 1.5 0 1.6 0 1.5 0 0 0 0 0 1.2 0.8 2.3
Peroxisomes -1.1 -3.0 -5.5 0 -6.0 0 -6.1 0 0 -4.9 -3.3 -2.9 7.1 3.2 3.5
SpindlePole 0 0.5 0 0 0 0 0 0 0 0 0 0 5.1 6.4 4.8
Vacuole 0 0 0 0 2.4 3.6 3.8 2.9 0 0 0 0 7.3 6.8 5.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.6577 6.9971 6.1662 5.9998 4.5467 5.752 11.3295 10.8286 11.4659 11.3152 10.3182 11.4103 10.8627 11.9202 11.8596 10.7869 11.7651 11.6857
Actin 0.0187 0.0114 0.0093 0.008 0.0004 0.0014 0.0028 0.0015 0.0041 0 0.0033 0.0004 0.0074 0.0063 0.0056 0.0017 0.0002 0.0032
Bud 0.0004 0.0002 0.0003 0.0001 0 0.0001 0.0001 0.0002 0.0003 0 0.0004 0 0.0002 0.0003 0.0002 0.0002 0 0
Bud Neck 0.0004 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0003 0.0001 0 0 0 0
Bud Periphery 0.0005 0.0004 0.0012 0.0002 0.0001 0.0002 0.0002 0.0005 0.0007 0 0.0009 0.0002 0.0009 0.0006 0.0005 0.0006 0.0001 0.0001
Bud Site 0.0018 0.0005 0.0008 0.0003 0 0.0001 0.0006 0.0003 0.0003 0 0.0004 0.0001 0.0002 0.0008 0.0001 0.0001 0 0
Cell Periphery 0.0002 0.0003 0.0004 0.0001 0 0.0001 0.0002 0.0003 0.0002 0 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0
Cytoplasm 0.004 0.0001 0.0003 0.0024 0 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0011 0.0001 0.0002 0 0 0
Cytoplasmic Foci 0.0165 0.0081 0.008 0.0015 0.0002 0.0004 0.0026 0.0028 0.0013 0 0.0018 0.0005 0.0114 0.0022 0.0024 0.0017 0.0002 0.0012
Eisosomes 0.0012 0.0003 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0002 0.0001 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0027 0.0001 0.0003 0.0027 0 0.0001 0.0003 0.0002 0.0001 0 0.0004 0.0005 0.0008 0.0001 0.0001 0 0 0
Endosome 0.0252 0.0079 0.016 0.0078 0.0008 0.003 0.0068 0.0084 0.0071 0.0007 0.0216 0.007 0.0187 0.0069 0.0089 0.0038 0.0021 0.0068
Golgi 0.0164 0.0366 0.0161 0.024 0.0025 0.0069 0.02 0.0342 0.0221 0.0031 0.0348 0.0209 0.03 0.0281 0.019 0.0229 0.006 0.0177
Lipid Particles 0.0465 0.0212 0.0125 0.015 0.005 0.0051 0.0092 0.0074 0.0053 0.0001 0.0213 0.0013 0.0298 0.0068 0.0029 0.0012 0.0005 0.0049
Mitochondria 0.7651 0.8946 0.9032 0.9134 0.9882 0.975 0.9413 0.9334 0.9491 0.9949 0.9031 0.963 0.8615 0.9214 0.9541 0.9642 0.983 0.9583
None 0.0247 0 0.0004 0.0008 0 0 0.0006 0 0.0001 0 0.0001 0.0001 0.0038 0 0.0001 0 0 0
Nuclear Periphery 0.0115 0.0002 0.0006 0.0115 0 0.0001 0.0025 0 0.0001 0 0.0005 0.0005 0.0027 0.0001 0 0 0 0
Nucleolus 0.0029 0.0019 0.0021 0.0002 0 0.0001 0.0005 0.0001 0.0001 0 0.0001 0.0014 0.0013 0.0003 0 0 0 0
Nucleus 0.0096 0.0003 0.0003 0.0034 0 0.0001 0.0003 0 0.0001 0 0.0003 0.0007 0.0009 0.0001 0 0 0 0
Peroxisomes 0.0348 0.009 0.0107 0.0033 0.0015 0.0015 0.0048 0.0072 0.0051 0.0001 0.0027 0.0007 0.0131 0.0126 0.0028 0.002 0.0006 0.0059
Punctate Nuclear 0.0113 0.0001 0.0003 0.0012 0 0 0.002 0 0 0 0.0001 0.0001 0.0087 0 0.0001 0 0 0
Vacuole 0.0019 0.0026 0.0051 0.001 0.0002 0.0008 0.0013 0.0009 0.001 0.0001 0.0041 0.0007 0.0021 0.0037 0.0008 0.0003 0.0009 0.0003
Vacuole Periphery 0.0038 0.0039 0.0114 0.0026 0.0009 0.0048 0.0035 0.0023 0.0027 0.0008 0.0039 0.0018 0.0045 0.009 0.0019 0.0009 0.0061 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.0607 13.2527 18.7315 21.5006 17.8483 15.5224 17.5473 24.3325 18.9958 19.8919
Translational Efficiency 1.5648 1.7298 1.3634 1.3435 1.1861 1.502 1.1732 0.8302 1.1623 1.0829

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Non-proteolytic ATPase of the AAA family; stimulates incorporation of the pyridoxal phosphate cofactor into Hem1p (5-aminolevulinic acid synthase); localized to the mitochondrial matrix; ortholog of vertebrate CLPX, which promotes erythropoiesis
Localization
Cell Percentages mitochondrion (98%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mcx1

Mcx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available