Standard name | |
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Human Ortholog | |
Description | Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 4 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 3 | 1 | 2 | 2 | 11 | 7 | 7 | 25 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 14 | 6 | 2 | 7 | 4 | 7 | 11 | 6 | 7 | 3 | 9 | 12 | 27 | 46 | 37 | 30 | 35 | 0 | 5 | 0 | 2 | 5 | 1 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 3 | 9 | 0 | 0 | 1 | 0 | 1 | 0 |
Endosome | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 7 | 7 | 5 | 1 | 3 | 3 | 4 | 3 | 4 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 0 | 0 | 1 |
Mitochondria | 9 | 154 | 1 | 4 | 28 | 19 | 26 | 20 | 103 | 58 | 90 | 96 | 1 | 7 | 2 | 15 | 8 | 5 | 1 | 6 | 2 | 1 | 1 | 2 |
Nucleus | 143 | 346 | 155 | 84 | 83 | 91 | 140 | 192 | 174 | 175 | 232 | 245 | 198 | 280 | 279 | 165 | 125 | 168 | 141 | 334 | 140 | 80 | 166 | 145 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 2 | 1 | 1 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 |
Nucleolus | 37 | 18 | 3 | 6 | 4 | 27 | 14 | 15 | 59 | 55 | 45 | 45 | 10 | 29 | 26 | 9 | 10 | 13 | 14 | 21 | 18 | 20 | 46 | 47 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 4 | 3 | 3 | 0 | 1 | 2 | 5 | 0 | 2 | 3 | 4 | 2 | 14 | 5 | 0 | 5 | 4 | 3 | 3 | 1 | 3 | 4 | 4 |
Vac/Vac Membrane | 5 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 1 | 6 | 14 | 13 | 14 | 2 | 15 | 7 | 5 | 9 | 16 |
Unique Cell Count | 158 | 402 | 165 | 89 | 95 | 111 | 159 | 221 | 229 | 217 | 275 | 293 | 215 | 322 | 340 | 228 | 180 | 233 | 169 | 403 | 180 | 122 | 249 | 237 |
Labelled Cell Count | 197 | 541 | 173 | 99 | 125 | 144 | 194 | 251 | 358 | 306 | 387 | 430 | 225 | 362 | 371 | 254 | 204 | 255 | 169 | 403 | 180 | 122 | 249 | 237 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 4.6 | 6.3 | 6.0 | 4.3 | 4.8 | 5.1 | 5.5 | 4.4 | 4.8 | 4.8 | 4.7 | 6.5 | 6.1 | 6.1 | 6.4 | 7.7 | 7.9 | 5.9 | 7.6 | 7.6 |
Std Deviation (1e-4) | 1.1 | 0.8 | 1.1 | 1.0 | 1.1 | 1.0 | 0.9 | 1.3 | 1.0 | 1.1 | 0.9 | 1.0 | 1.3 | 1.2 | 1.5 | 1.8 | 1.9 | 2.3 | 1.3 | 1.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.54 | -0.39 | -0.32 | -0.21 | -0.54 | -0.4 | -0.41 | -0.42 | 0.03 | -0.04 | -0.05 | 0.02 | 0.28 | 0.32 | -0.09 | 0.26 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 2.8 | 3.1 | 2.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.9336 | 5.2754 | 4.8898 | 5.4918 | 5.1206 | 5.2834 | 5.9656 | 5.5016 | 5.7471 | 5.5628 | 6.0262 | 5.7742 | 3.0117 | 3.115 | 2.7669 | 2.8623 | 2.9311 | 3.058 |
Actin | 0.0186 | 0 | 0.0007 | 0 | 0.0041 | 0.0014 | 0.0023 | 0.0169 | 0.0031 | 0.02 | 0.0093 | 0.002 | 0.0186 | 0.0004 | 0.0072 | 0 | 0.004 | 0.0044 |
Bud | 0.0007 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0015 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0002 |
Bud Neck | 0.0032 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0023 | 0.0012 | 0.0004 | 0.0053 | 0.0035 | 0.0025 | 0.0009 | 0.0026 | 0.0001 | 0.0035 | 0 | 0.0017 | 0.002 |
Bud Periphery | 0.0017 | 0 | 0.0018 | 0 | 0.0004 | 0.0006 | 0.0008 | 0.0001 | 0.0007 | 0.0001 | 0.0048 | 0.0003 | 0.0021 | 0.0001 | 0.0004 | 0 | 0.0029 | 0.0009 |
Bud Site | 0.0058 | 0.0001 | 0.0006 | 0 | 0.0014 | 0.0004 | 0.0012 | 0.0112 | 0.0018 | 0.0036 | 0.0036 | 0.0002 | 0.0032 | 0.0004 | 0.0017 | 0 | 0.002 | 0.0002 |
Cell Periphery | 0.0006 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.001 | 0 | 0.0006 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0043 | 0 | 0.0002 | 0.0001 | 0.0014 | 0.0001 | 0.0034 | 0.0002 | 0.0007 | 0.0093 | 0.0078 | 0.0004 | 0.0073 | 0.0001 | 0.0063 | 0 | 0.0298 | 0.0001 |
Cytoplasmic Foci | 0.0073 | 0 | 0.0017 | 0 | 0.0103 | 0.0005 | 0.001 | 0.0005 | 0.0039 | 0.0052 | 0.0051 | 0.0008 | 0.0125 | 0.0001 | 0.0082 | 0 | 0.0078 | 0.0009 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0012 | 0 | 0.0001 | 0 | 0.0011 | 0.0002 | 0.006 | 0 | 0.0015 | 0.0033 | 0.0025 | 0.0007 | 0.0029 | 0 | 0.003 | 0 | 0.004 | 0.0002 |
Endosome | 0.0133 | 0 | 0.0012 | 0 | 0.0181 | 0.0019 | 0.0078 | 0.0001 | 0.0049 | 0.0012 | 0.0169 | 0.0034 | 0.0097 | 0.0004 | 0.004 | 0 | 0.0197 | 0.0034 |
Golgi | 0.0048 | 0 | 0.0005 | 0 | 0.0038 | 0.0013 | 0.0022 | 0.0004 | 0.0024 | 0.0021 | 0.0029 | 0.001 | 0.0039 | 0.0001 | 0.0022 | 0 | 0.0062 | 0.0027 |
Lipid Particles | 0.0105 | 0 | 0.0024 | 0 | 0.0172 | 0.0009 | 0.0013 | 0.0001 | 0.0052 | 0.0022 | 0.0021 | 0.0019 | 0.0148 | 0 | 0.0067 | 0 | 0.0146 | 0.0063 |
Mitochondria | 0.009 | 0.0001 | 0.0025 | 0.0001 | 0.0033 | 0.0117 | 0.0022 | 0.0001 | 0.0026 | 0.0003 | 0.0121 | 0.0009 | 0.0104 | 0.0114 | 0.0022 | 0.0001 | 0.018 | 0.0047 |
None | 0.0015 | 0 | 0.0002 | 0.0007 | 0.0018 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0006 | 0.0359 | 0.0001 | 0.0034 | 0.0001 | 0.0014 | 0 | 0.0036 | 0.0002 |
Nuclear Periphery | 0.0105 | 0.0002 | 0.0065 | 0.0004 | 0.0472 | 0.0005 | 0.0109 | 0.0003 | 0.0032 | 0.0031 | 0.0117 | 0.0063 | 0.0193 | 0.0006 | 0.0018 | 0.0004 | 0.0172 | 0.0015 |
Nucleolus | 0.0378 | 0.0229 | 0.024 | 0.0164 | 0.0764 | 0.0902 | 0.0232 | 0.0345 | 0.0149 | 0.0059 | 0.0598 | 0.0605 | 0.0281 | 0.023 | 0.0211 | 0.0114 | 0.0898 | 0.0653 |
Nucleus | 0.8346 | 0.9757 | 0.9519 | 0.9801 | 0.7867 | 0.8827 | 0.9255 | 0.9305 | 0.9426 | 0.9144 | 0.7952 | 0.9162 | 0.8199 | 0.9613 | 0.9208 | 0.9875 | 0.7272 | 0.8998 |
Peroxisomes | 0.0128 | 0 | 0.0022 | 0 | 0.0151 | 0.0011 | 0.0002 | 0.0002 | 0.0024 | 0.0231 | 0.0021 | 0.0005 | 0.0206 | 0.0002 | 0.0045 | 0 | 0.0238 | 0.0013 |
Punctate Nuclear | 0.0135 | 0.0008 | 0.0009 | 0.0022 | 0.0083 | 0.0027 | 0.0032 | 0.004 | 0.0027 | 0.0012 | 0.0175 | 0.0032 | 0.0121 | 0.0013 | 0.0034 | 0.0003 | 0.0201 | 0.0038 |
Vacuole | 0.0056 | 0 | 0.0012 | 0 | 0.0009 | 0.0006 | 0.0044 | 0.0001 | 0.0008 | 0.0001 | 0.0038 | 0.0003 | 0.005 | 0.0001 | 0.0005 | 0 | 0.0041 | 0.0009 |
Vacuole Periphery | 0.0024 | 0 | 0.0004 | 0 | 0.0017 | 0.0003 | 0.0019 | 0 | 0.0005 | 0 | 0.0016 | 0.0003 | 0.0016 | 0.0001 | 0.0002 | 0 | 0.0024 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.2592 | 8.6524 | 11.2358 | 13.5327 | 15.6865 | 7.2552 | 12.5759 | 11.0712 | 22.9236 | 13.3148 |
Translational Efficiency | 3.7549 | 2.6299 | 2.3325 | 2.8697 | 2.058 | 4.3065 | 1.9103 | 3.6207 | 1.4651 | 1.7929 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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