Standard name | |
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Human Ortholog | |
Description | Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 71 | – | 141 | 123 | 145 | 111 | 216 | 162 | 130 | 146 | 137 | 167 | 51 | 146 | 124 | 37 | 7 | 20 | 112 | 45 | 244 | 129 | 173 | 224 |
Bud | 21 | – | 45 | 34 | 87 | 51 | 111 | 54 | 50 | 65 | 59 | 55 | 11 | 27 | 8 | 4 | 1 | 2 | 6 | 3 | 11 | 13 | 11 | 13 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 2 |
Bud Site | 3 | – | 1 | 6 | 9 | 5 | 6 | 6 | 1 | 6 | 5 | 7 | 0 | 0 | 0 | 1 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 4 | – | 13 | 16 | 10 | 11 | 25 | 17 | 19 | 9 | 14 | 25 | 8 | 24 | 28 | 4 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 2 |
Cytoplasm | 15 | – | 20 | 20 | 18 | 13 | 18 | 11 | 9 | 6 | 15 | 6 | 4 | 9 | 10 | 22 | 1 | 5 | 1 | 1 | 4 | 0 | 5 | 0 |
Endoplasmic Reticulum | 8 | – | 9 | 5 | 5 | 4 | 17 | 17 | 30 | 20 | 21 | 21 | 3 | 15 | 16 | 5 | 0 | 3 | 1 | 0 | 0 | 2 | 4 | 1 |
Endosome | 10 | – | 11 | 5 | 29 | 20 | 24 | 13 | 9 | 11 | 9 | 13 | 10 | 5 | 7 | 1 | 0 | 1 | 3 | 0 | 2 | 6 | 9 | 4 |
Golgi | 18 | – | 67 | 60 | 49 | 38 | 69 | 112 | 119 | 73 | 58 | 102 | 38 | 174 | 171 | 44 | 8 | 25 | 9 | 4 | 12 | 18 | 16 | 19 |
Mitochondria | 44 | – | 44 | 85 | 95 | 184 | 301 | 157 | 174 | 191 | 163 | 219 | 17 | 10 | 4 | 12 | 4 | 7 | 1 | 1 | 0 | 5 | 3 | 1 |
Nucleus | 1 | – | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | – | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 1 | 5 | 14 | 10 | 14 | 4 | 6 | 9 | 9 | 11 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 6 | – | 61 | 64 | 80 | 75 | 77 | 83 | 35 | 50 | 18 | 53 | 11 | 14 | 12 | 2 | 0 | 0 | 2 | 0 | 12 | 3 | 3 | 4 |
SpindlePole | 1 | – | 10 | 13 | 13 | 9 | 9 | 13 | 5 | 11 | 4 | 5 | 2 | 4 | 2 | 5 | 1 | 2 | 1 | 0 | 1 | 1 | 1 | 0 |
Vac/Vac Membrane | 0 | – | 0 | 1 | 1 | 1 | 2 | 3 | 1 | 3 | 2 | 3 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 1 |
Unique Cell Count | 119 | 244 | 241 | 312 | 327 | 550 | 381 | 347 | 366 | 329 | 428 | 80 | 248 | 242 | 101 | 16 | 46 | 142 | 59 | 293 | 192 | 244 | 280 | |
Labelled Cell Count | 202 | 424 | 438 | 555 | 532 | 891 | 653 | 589 | 601 | 515 | 688 | 157 | 430 | 383 | 141 | 22 | 69 | 142 | 59 | 293 | 192 | 244 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.5 | – | 22.9 | 18.9 | 19.7 | 20.9 | 19.6 | 22.4 | 23.2 | 20.9 | 21.9 | 21.6 | 22.5 | 23.0 | 24.4 | 32.9 | 34.7 | 33.6 | 24.0 | 22.1 | 23.6 |
Std Deviation (1e-4) | 8.8 | – | 11.6 | 10.8 | 10.4 | 12.0 | 11.1 | 12.8 | 14.0 | 12.3 | 13.5 | 14.4 | 12.8 | 11.3 | 11.6 | 18.2 | 17.5 | 12.3 | 12.4 | 10.7 | 10.3 |
Intensity Change (Log2) | – | – | – | -0.28 | -0.22 | -0.13 | -0.23 | -0.03 | 0.02 | -0.13 | -0.06 | -0.09 | -0.03 | 0.01 | 0.09 | 0.52 | 0.6 | 0.55 | 0.07 | -0.05 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.5 | -2.6 | -5.7 | -4.8 | -3.7 | -4.9 | -4.3 | -3.8 | -4.7 | 0.9 | 0.2 | -1.4 | -3.6 | -1.1 | -1.8 |
Bud | -1.3 | 2.6 | -0.9 | 0.6 | -1.4 | -1.3 | -0.2 | -0.2 | -2.0 | -1.0 | -2.4 | -5.4 | -3.5 | 0 | -2.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.6 | -1.2 | -1.2 | -0.5 | -0.5 | 0.1 | -1.9 | -0.6 | 0.3 | 1.5 | 1.8 | 2.5 | 0 | 0 | 0 |
Cytoplasm | 0 | -1.1 | -2.1 | -3.0 | -3.0 | -3.1 | -3.9 | -1.8 | -4.4 | -0.9 | -2.2 | -1.9 | 3.5 | 0 | 0 |
Endoplasmic Reticulum | -1.1 | -1.6 | -2.0 | -0.4 | 0.5 | 2.4 | 1.0 | 1.4 | 0.7 | 0 | 1.2 | 1.5 | 0 | 0 | 0 |
Endosome | -1.5 | 2.2 | 0.8 | -0.1 | -0.7 | -1.3 | -1.0 | -1.1 | -1.0 | 2.5 | -1.6 | -0.9 | 0 | 0 | 0 |
Golgi | -0.6 | -3.4 | -4.8 | -5.1 | 0.5 | 1.8 | -2.2 | -2.8 | -1.0 | 3.3 | 9.5 | 9.5 | 2.9 | 0 | 3.6 |
Mitochondria | 4.3 | 3.4 | 9.2 | 9.6 | 6.1 | 8.0 | 8.5 | 7.8 | 8.5 | 0.6 | -5.0 | -6.1 | -1.4 | 0 | -0.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.4 | 0.2 | -0.6 | -3.8 | -0.9 | -4.8 | -3.6 | -6.7 | -4.2 | -2.1 | -6.0 | -6.2 | -5.0 | 0 | -3.8 |
SpindlePole | 0.7 | 0 | -0.9 | -2.1 | -0.4 | -2.0 | -0.7 | -2.2 | -2.5 | 0 | -1.7 | -2.3 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 10.331 | 15.6772 | 13.1406 | 8.8616 | 12.8136 | 14.8597 | 14.9453 | 19.2954 | 18.6879 | 16.0004 | 17.6392 | 19.3751 | 9.8207 | 10.9428 | 11.3192 | 11.7204 | 9.2811 | 11.473 |
Actin | 0.6639 | 0.8586 | 0.8484 | 0.9724 | 0.682 | 0.9357 | 0.7484 | 0.7902 | 0.8498 | 0.7608 | 0.8256 | 0.9125 | 0.7547 | 0.8861 | 0.893 | 0.7091 | 0.8241 | 0.9156 |
Bud | 0.0011 | 0.0045 | 0.0051 | 0.0055 | 0.0036 | 0.0005 | 0.0021 | 0.003 | 0.0136 | 0.0059 | 0.0038 | 0.0002 | 0.002 | 0.0034 | 0.017 | 0.0035 | 0.0005 | 0.0003 |
Bud Neck | 0.0022 | 0.0009 | 0.002 | 0.0002 | 0.0005 | 0.0075 | 0.0021 | 0.0032 | 0.0044 | 0.01 | 0.0041 | 0.0185 | 0.0036 | 0.001 | 0.0013 | 0.0014 | 0.0011 | 0.0118 |
Bud Periphery | 0.0009 | 0.0024 | 0.0077 | 0.0043 | 0.0222 | 0.0007 | 0.0023 | 0.002 | 0.0088 | 0.0087 | 0.0056 | 0.0003 | 0.0017 | 0.0022 | 0.0207 | 0.0041 | 0.0004 | 0.0004 |
Bud Site | 0.0209 | 0.033 | 0.0163 | 0.0133 | 0.0015 | 0.0011 | 0.0067 | 0.0692 | 0.021 | 0.0078 | 0.0101 | 0.0003 | 0.0197 | 0.0593 | 0.0132 | 0.0052 | 0.0008 | 0.0006 |
Cell Periphery | 0.0003 | 0.0004 | 0.0005 | 0.0001 | 0.0011 | 0.0001 | 0.0005 | 0.0007 | 0.0004 | 0.0017 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.0187 | 0.016 | 0.0064 | 0.0002 | 0.0008 | 0.0016 | 0.006 | 0.0018 | 0.0072 | 0.0037 | 0.0026 | 0.0001 | 0.0304 | 0.0024 | 0.0122 | 0.0023 | 0.0023 | 0.0004 |
Cytoplasmic Foci | 0.0592 | 0.0251 | 0.0187 | 0.0006 | 0.0225 | 0.0046 | 0.0223 | 0.0335 | 0.0089 | 0.0486 | 0.0318 | 0.0011 | 0.0305 | 0.0111 | 0.0093 | 0.0202 | 0.0194 | 0.0183 |
Eisosomes | 0.0054 | 0.0068 | 0.0188 | 0.0004 | 0.1022 | 0.0012 | 0.0323 | 0.0534 | 0.0179 | 0.0013 | 0.0007 | 0.0399 | 0.0017 | 0.0003 | 0.0009 | 0.0291 | 0.0005 | 0.0019 |
Endoplasmic Reticulum | 0.0019 | 0 | 0.003 | 0 | 0.0024 | 0.0017 | 0.0077 | 0.0002 | 0.0009 | 0.0008 | 0.0056 | 0 | 0.0141 | 0.0001 | 0.0001 | 0.0025 | 0.0023 | 0.0005 |
Endosome | 0.0423 | 0.0004 | 0.0118 | 0 | 0.0508 | 0.0106 | 0.0575 | 0.0016 | 0.0148 | 0.0456 | 0.0343 | 0.0008 | 0.043 | 0.0043 | 0.0018 | 0.0781 | 0.0475 | 0.0056 |
Golgi | 0.0392 | 0.0039 | 0.0122 | 0.0009 | 0.0618 | 0.0082 | 0.0335 | 0.0062 | 0.024 | 0.0465 | 0.0194 | 0.0203 | 0.0233 | 0.0185 | 0.0158 | 0.0608 | 0.0669 | 0.0141 |
Lipid Particles | 0.0198 | 0.0004 | 0.0025 | 0 | 0.0309 | 0.0035 | 0.0174 | 0.0007 | 0.0044 | 0.0196 | 0.0245 | 0.0003 | 0.0073 | 0.0005 | 0.0002 | 0.0306 | 0.0199 | 0.0062 |
Mitochondria | 0.0165 | 0.0008 | 0.0181 | 0.0005 | 0.0048 | 0.0019 | 0.0125 | 0.0063 | 0.0136 | 0.0125 | 0.0075 | 0.0005 | 0.0054 | 0.0006 | 0.0008 | 0.0077 | 0.0063 | 0.0069 |
None | 0.0304 | 0.0011 | 0.0063 | 0 | 0.0005 | 0.0002 | 0.0078 | 0.0001 | 0.0003 | 0.0001 | 0.0021 | 0 | 0.0032 | 0 | 0.0044 | 0.0004 | 0.0005 | 0.0005 |
Nuclear Periphery | 0.0037 | 0 | 0.0039 | 0 | 0.0018 | 0.0076 | 0.0148 | 0.0001 | 0.0004 | 0.0007 | 0.0071 | 0 | 0.0238 | 0 | 0.0001 | 0.0036 | 0.0012 | 0.0089 |
Nucleolus | 0.0109 | 0.0002 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0005 | 0.0006 | 0 | 0.0018 | 0 | 0 | 0.0003 | 0.0001 | 0.0003 |
Nucleus | 0.0115 | 0.0001 | 0.0035 | 0 | 0.0002 | 0.0007 | 0.0022 | 0.0001 | 0.0002 | 0.0007 | 0.0015 | 0 | 0.0103 | 0 | 0.0005 | 0.0006 | 0.0001 | 0.0005 |
Peroxisomes | 0.0388 | 0.045 | 0.0066 | 0.0012 | 0.0072 | 0.0099 | 0.0062 | 0.0272 | 0.0064 | 0.0142 | 0.0052 | 0.005 | 0.0134 | 0.0098 | 0.0084 | 0.0273 | 0.0028 | 0.0048 |
Punctate Nuclear | 0.0092 | 0.0003 | 0.0055 | 0 | 0.0018 | 0.0013 | 0.0057 | 0.0002 | 0.0005 | 0.001 | 0.0025 | 0 | 0.0028 | 0.0001 | 0.0001 | 0.0008 | 0.0006 | 0.0006 |
Vacuole | 0.0023 | 0.0001 | 0.001 | 0 | 0.0005 | 0.0004 | 0.0084 | 0.0001 | 0.0016 | 0.0076 | 0.0031 | 0 | 0.0044 | 0.0001 | 0.0001 | 0.0075 | 0.0011 | 0.0003 |
Vacuole Periphery | 0.001 | 0 | 0.0008 | 0 | 0.0007 | 0.0007 | 0.003 | 0 | 0.0006 | 0.0016 | 0.0019 | 0 | 0.0023 | 0.0001 | 0 | 0.0048 | 0.0015 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 100.3095 | 78.5685 | 96.9574 | 109.9455 | 98.4707 | 104.0732 | 104.9133 | 104.0286 | 114.08 | 109.9096 |
Translational Efficiency | 1.2324 | 1.2318 | 1.1019 | 1.0311 | 1.1 | 1.4452 | 1.1841 | 1.1198 | 1.1536 | 1.0291 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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