Standard name | |
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Human Ortholog | |
Description | Methyltransferase; catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; nuclear protein that relocalizes to the cytosol in response to hypoxia |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 |
Bud | 0 | 0 | 0 | 0 | 3 | 3 | 10 | 3 | 10 | 14 | 5 | 5 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 7 | 2 | 2 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 2 | 2 | 2 | 6 | 11 | 9 | 7 | 19 | 29 | 23 | 32 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 11 | 25 | 24 | 39 | 16 | 21 | 29 | 10 | 9 | 10 | 11 | 30 | 66 | 84 | 42 | 47 | 30 | 0 | 2 | 2 | 3 | 3 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 6 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | 0 | 4 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 4 | 4 | 1 | 1 | 1 | 0 | 1 | 7 | 1 | 8 | 3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 1 |
Mitochondria | 22 | 25 | 22 | 25 | 58 | 204 | 307 | 153 | 202 | 361 | 278 | 312 | 0 | 0 | 0 | 4 | 11 | 4 | 0 | 3 | 3 | 2 | 4 | 10 |
Nucleus | 127 | 390 | 764 | 582 | 655 | 880 | 933 | 813 | 784 | 910 | 667 | 725 | 191 | 305 | 316 | 445 | 416 | 260 | 122 | 390 | 756 | 312 | 349 | 315 |
Nuclear Periphery | 4 | 1 | 4 | 10 | 3 | 4 | 12 | 12 | 30 | 16 | 12 | 10 | 0 | 2 | 1 | 4 | 4 | 1 | 0 | 0 | 3 | 0 | 3 | 3 |
Nucleolus | 28 | 42 | 55 | 27 | 72 | 257 | 291 | 288 | 254 | 363 | 278 | 270 | 1 | 3 | 5 | 14 | 10 | 6 | 7 | 9 | 29 | 18 | 45 | 46 |
Peroxisomes | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 5 | 2 | 2 | 0 | 0 | 6 | 5 | 5 | 2 | 6 | 0 | 5 | 6 | 0 | 3 | 0 | 0 | 3 | 5 | 1 | 3 | 7 |
Vac/Vac Membrane | 0 | 2 | 5 | 4 | 5 | 4 | 13 | 7 | 3 | 9 | 2 | 4 | 2 | 9 | 8 | 8 | 3 | 6 | 2 | 14 | 37 | 16 | 63 | 55 |
Unique Cell Count | 140 | 423 | 825 | 636 | 765 | 1077 | 1238 | 1098 | 1017 | 1269 | 938 | 1014 | 223 | 388 | 418 | 507 | 475 | 305 | 140 | 432 | 867 | 364 | 495 | 460 |
Labelled Cell Count | 182 | 473 | 887 | 678 | 847 | 1381 | 1596 | 1320 | 1318 | 1723 | 1278 | 1380 | 228 | 395 | 432 | 526 | 496 | 317 | 140 | 432 | 867 | 364 | 495 | 460 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | 7.0 | 6.7 | 6.3 | 6.2 | 5.3 | 5.4 | 5.6 | 5.2 | 5.1 | 4.8 | 5.0 | 9.0 | 8.7 | 8.2 | 10.1 | 9.1 | 9.8 | 9.6 | 10.2 | 10.3 |
Std Deviation (1e-4) | 1.0 | 1.3 | 1.3 | 1.6 | 1.5 | 1.2 | 1.4 | 1.5 | 1.2 | 1.2 | 1.3 | 1.3 | 2.5 | 2.4 | 2.9 | 2.9 | 2.3 | 2.1 | 2.0 | 2.5 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.11 | -0.33 | -0.31 | -0.25 | -0.36 | -0.4 | -0.48 | -0.41 | 0.44 | 0.39 | 0.3 | 0.59 | 0.45 | 0.56 | 0.53 | 0.61 | 0.63 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 2.0 | 0 | 0 | 3.3 | 3.8 | 0 | 4.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | 2.1 | -2.3 | -2.0 | -0.5 | -3.2 | -4.1 | -3.0 | -3.0 | 6.2 | 8.6 | 10.0 | 4.3 | 5.2 | 4.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.4 | 4.5 | 10.9 | 13.5 | 8.5 | 11.2 | 14.9 | 0 | 15.5 | 0 | -3.2 | -3.4 | -2.4 | -0.4 | -1.3 |
Nucleus | -0.8 | -4.5 | -6.9 | -10.0 | -10.5 | -9.0 | -11.7 | -11.5 | -11.5 | -3.2 | -7.0 | -8.4 | -3.0 | -3.0 | -3.8 |
Nuclear Periphery | 2.1 | 0 | 0 | 1.2 | 1.5 | 3.9 | 1.8 | 0 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.0 | 2.0 | 10.0 | 10.0 | 11.1 | 10.5 | 12.3 | 0 | 11.2 | -3.7 | -4.5 | -4.3 | -3.1 | -3.6 | -3.1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 1.1 | 0.1 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.9265 | 8.3979 | 8.3662 | 7.3751 | 7.4214 | 7.8843 | 6.2918 | 6.94 | 6.4412 | 5.7937 | 5.3933 | 5.9912 | 9.7331 | 10.0715 | 9.7493 | 8.9257 | 8.8701 | 9.9047 |
Actin | 0.0196 | 0 | 0.0014 | 0 | 0.0002 | 0.0022 | 0.073 | 0 | 0.0041 | 0.0684 | 0.0008 | 0.0094 | 0.0006 | 0 | 0.0008 | 0 | 0 | 0.0001 |
Bud | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0015 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0008 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0044 | 0.0001 | 0.0001 | 0 | 0.0134 | 0.0006 | 0.0033 | 0 | 0.0021 | 0.0002 | 0.0004 | 0.001 | 0.0004 | 0 | 0.0001 | 0 | 0.0003 | 0.0002 |
Bud Periphery | 0.0006 | 0 | 0.0001 | 0 | 0.0006 | 0.0002 | 0.0031 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0008 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0039 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0064 | 0 | 0.0117 | 0.0003 | 0.0007 | 0.0029 | 0.0003 | 0 | 0 | 0.0001 | 0.0003 | 0 |
Cell Periphery | 0.0007 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0007 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0117 | 0.0016 | 0.0002 | 0 | 0.0007 | 0.0007 | 0.0041 | 0 | 0.003 | 0.0005 | 0.0006 | 0.0014 | 0.0021 | 0.0004 | 0.0009 | 0.0001 | 0.0006 | 0.0001 |
Cytoplasmic Foci | 0.0039 | 0 | 0.0006 | 0 | 0 | 0.0002 | 0.0192 | 0 | 0.0039 | 0.0119 | 0.0012 | 0.0034 | 0.0005 | 0.0001 | 0.0014 | 0 | 0.0002 | 0.0001 |
Eisosomes | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0031 | 0.0001 | 0.0004 | 0 | 0.0005 | 0.0006 | 0.0052 | 0 | 0.0012 | 0.0003 | 0.0019 | 0.0021 | 0.0005 | 0 | 0.0013 | 0 | 0.0001 | 0 |
Endosome | 0.0086 | 0.0001 | 0.0016 | 0 | 0.0006 | 0.0011 | 0.0389 | 0 | 0.0009 | 0.0037 | 0.0025 | 0.0102 | 0.0014 | 0 | 0.0014 | 0 | 0.0002 | 0 |
Golgi | 0.0015 | 0 | 0.0005 | 0 | 0 | 0.0002 | 0.0139 | 0 | 0.0007 | 0.009 | 0.0007 | 0.0053 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Lipid Particles | 0.0032 | 0 | 0.0018 | 0 | 0.0001 | 0.0001 | 0.0198 | 0 | 0.0023 | 0.0061 | 0.0102 | 0.0047 | 0.0007 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Mitochondria | 0.0019 | 0.0001 | 0.0007 | 0 | 0.0005 | 0.0004 | 0.0152 | 0.0001 | 0.0005 | 0.0009 | 0.0359 | 0.0066 | 0.0077 | 0 | 0.0031 | 0.0055 | 0.0002 | 0.0001 |
None | 0.0135 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0034 | 0 | 0.0017 | 0.0003 | 0.0009 | 0.0009 | 0.0034 | 0 | 0.0015 | 0.0001 | 0.0002 | 0 |
Nuclear Periphery | 0.004 | 0.0045 | 0.0058 | 0.0003 | 0.0081 | 0.0033 | 0.0326 | 0.0006 | 0.0035 | 0.0004 | 0.0278 | 0.0066 | 0.0076 | 0.0014 | 0.0037 | 0.0004 | 0.0043 | 0.0007 |
Nucleolus | 0.0156 | 0.0131 | 0.0143 | 0.0067 | 0.0863 | 0.0487 | 0.0399 | 0.0129 | 0.0101 | 0.0043 | 0.0368 | 0.0407 | 0.0335 | 0.0141 | 0.0209 | 0.027 | 0.0561 | 0.0435 |
Nucleus | 0.8898 | 0.9614 | 0.9671 | 0.9928 | 0.8686 | 0.9388 | 0.6654 | 0.9859 | 0.9458 | 0.8776 | 0.8547 | 0.8954 | 0.9224 | 0.962 | 0.9498 | 0.9456 | 0.9205 | 0.9342 |
Peroxisomes | 0.0062 | 0 | 0.0005 | 0 | 0 | 0 | 0.0102 | 0 | 0.0021 | 0.0152 | 0.0005 | 0.0022 | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Punctate Nuclear | 0.0017 | 0.0177 | 0.0039 | 0.0001 | 0.0158 | 0.002 | 0.0279 | 0.0003 | 0.0053 | 0.0006 | 0.0088 | 0.0034 | 0.0165 | 0.0217 | 0.0133 | 0.02 | 0.0161 | 0.0208 |
Vacuole | 0.0046 | 0.0008 | 0.0002 | 0 | 0.0031 | 0.0003 | 0.0081 | 0 | 0.0004 | 0 | 0.0044 | 0.0009 | 0.0008 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0003 | 0 | 0.0008 | 0.0001 | 0.008 | 0 | 0.0003 | 0 | 0.009 | 0.0008 | 0.0007 | 0 | 0.0006 | 0.0005 | 0.0001 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.0413 | 31.6002 | 29.0317 | 32.4388 | 22.916 | 25.2356 | 32.3312 | 34.4008 | 36.1819 | 30.2038 |
Translational Efficiency | 1.5546 | 1.032 | 1.2353 | 1.1137 | 1.3517 | 1.4169 | 1.1031 | 1.119 | 1.0392 | 1.1181 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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