Standard name
Human Ortholog
Description RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.08 0.08 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0.07 0.11 0.12 0.06 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.06 0.06 0.06 0.27 0.25 0.24 0.18 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.92 0.95 0.94 0.93 0.97 0.91 0.93 0.92 0.9 0.86 0.9 0.89 0.94 0.91 0.87 0.81 0.89 0.83 0.87 0.92 0.89 0.86 0.79 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.07 0 0 0 0.1 0.13 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1
Bud 0 1 0 0 1 1 5 2 1 4 4 3 0 0 1 0 0 0 3 3 2 1 3 9
Bud Neck 0 0 0 0 0 0 1 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 1 0 0 1 6 4 11 10 39 38 30 1 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 8 5 16 16 15 21 27 11 8 13 8 12 11 32 60 42 14 16 0 0 3 0 0 0
Endoplasmic Reticulum 0 1 2 1 0 0 1 1 0 1 0 0 0 1 0 7 3 3 0 0 1 0 1 2
Endosome 0 2 1 0 0 2 0 2 0 1 0 0 1 3 1 1 1 0 2 5 2 0 2 4
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 1 3 2 1 0 3 2
Mitochondria 9 13 3 4 4 29 39 30 103 117 109 96 12 6 6 9 3 8 1 4 4 0 2 8
Nucleus 158 294 305 280 503 418 603 480 341 401 414 474 337 410 484 290 194 122 154 286 292 132 299 310
Nuclear Periphery 1 1 0 0 3 20 5 12 4 15 9 14 7 9 3 2 2 0 0 0 0 0 0 2
Nucleolus 2 0 0 1 0 5 9 11 9 42 20 23 4 4 7 2 1 2 1 0 0 1 8 22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 2 1 3 3 2 2 4 1 3 4 5 1 1 3 3 3 2 1 0 0 0 2 3
Vac/Vac Membrane 2 4 1 0 3 8 2 6 1 5 3 8 1 3 5 24 5 11 4 6 14 16 49 74
Unique Cell Count 171 308 326 300 520 458 648 520 380 465 460 535 357 449 554 357 217 147 177 312 329 154 378 449
Labelled Cell Count 180 324 329 306 536 513 699 571 478 641 610 666 376 470 571 383 226 165 177 312 329 154 378 449


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 6.2 6.9 6.3 6.6 7.5 6.2 6.1 5.1 5.3 5.0 5.9 6.8 6.3 6.4 9.2 9.9 9.2 6.0 5.8 6.4
Std Deviation (1e-4) 1.3 1.1 1.3 1.6 1.4 12.9 4.2 4.5 4.5 4.5 3.5 7.8 4.4 1.3 1.6 2.4 2.2 2.3 1.7 1.2 1.5
Intensity Change (Log2) – – – -0.13 -0.06 0.1 -0.16 -0.18 -0.44 -0.38 -0.48 -0.22 -0.03 -0.14 -0.11 0.41 0.52 0.41 -0.22 -0.26 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 5.4 5.3 4.4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -1.5 -0.2 -0.5 -2.2 -2.0 -1.6 -2.5 -2.1 -1.2 1.3 3.0 3.2 0.8 2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 3.7 3.5 9.7 9.4 9.0 7.6 2.2 0 0 1.6 0 0
Nucleus -0.1 2.2 -1.2 -0.3 -0.7 -1.8 -3.3 -1.8 -2.4 0.5 -1.2 -2.9 -4.8 -1.7 -3.6
Nuclear Periphery 0 0 3.8 0 0 0 3.3 0 2.9 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 5.6 3.8 3.8 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 4.5 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – – – – –
Bud Periphery – – – – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – – – – –
Cytoplasmic Foci – – – – – – – – – – – – – – – – – –
Eisosomes – – – – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – – – – –
Lipid Particles – – – – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – – – – –
None – – – – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – – – – –
Punctate Nuclear – – – – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – – – – –
Vacuole Periphery – – – – – – – – – – – – – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.9233 9.2835 5.7407 10.1269 6.7323 9.0818 7.5288 7.5232 11.2299 10.5362
Translational Efficiency 0.6825 0.4639 0.729 0.4996 0.7038 0.5712 0.6257 0.5889 0.4131 0.559

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization –
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Prp5

Prp5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prp5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available