ORF | |
---|---|
Human Ortholog | |
Description | Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 10 | 9 | 1 | 2 | 1 | 4 | 6 | 3 | 0 | 6 | 2 | 0 | 0 | 1 | 0 | – | 0 | 3 | 6 | 4 | 0 | 3 | 4 |
Bud | 0 | 8 | 2 | 0 | 1 | 2 | 6 | 4 | 9 | 10 | 19 | 3 | 2 | 0 | 0 | 0 | – | 0 | 0 | 4 | 1 | 0 | 2 | 4 |
Bud Neck | 0 | 2 | 9 | 2 | 16 | 14 | 21 | 13 | 12 | 3 | 5 | 5 | 0 | 0 | 0 | 0 | – | 0 | 2 | 4 | 2 | 3 | 5 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 17 | 2 | 3 | 3 | 0 | 5 | 2 | 5 | 3 | 4 | 3 | 1 | 2 | 0 | – | 1 | 0 | 1 | 2 | 0 | 1 | 2 |
Cytoplasm | 216 | 260 | 254 | 194 | 196 | 172 | 159 | 132 | 110 | 102 | 66 | 69 | 177 | 179 | 212 | 54 | – | 16 | 194 | 220 | 182 | 51 | 104 | 114 |
Endoplasmic Reticulum | 7 | 8 | 4 | 1 | 0 | 11 | 2 | 8 | 7 | 9 | 17 | 19 | 1 | 3 | 1 | 3 | – | 0 | 2 | 3 | 4 | 2 | 5 | 5 |
Endosome | 8 | 2 | 0 | 0 | 3 | 5 | 2 | 1 | 0 | 1 | 0 | 1 | 2 | 2 | 5 | 1 | – | 0 | 17 | 10 | 4 | 2 | 3 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 1 | 0 | 0 | 0 | 2 |
Mitochondria | 1 | 12 | 0 | 3 | 2 | 31 | 79 | 26 | 185 | 203 | 238 | 81 | 2 | 0 | 6 | 2 | – | 2 | 31 | 66 | 103 | 21 | 60 | 54 |
Nucleus | 0 | 2 | 0 | 2 | 1 | 2 | 3 | 8 | 4 | 3 | 6 | 7 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 5 | 22 | 20 | 5 | 10 | 24 | 15 | 14 | 1 | 0 | 0 | 0 | – | 0 | 1 | 3 | 3 | 0 | 0 | 2 |
Vac/Vac Membrane | 6 | 4 | 2 | 0 | 7 | 4 | 7 | 8 | 8 | 3 | 3 | 6 | 0 | 3 | 1 | 0 | – | 1 | 6 | 27 | 10 | 6 | 18 | 22 |
Unique Cell Count | 235 | 289 | 274 | 198 | 218 | 250 | 265 | 195 | 299 | 308 | 312 | 187 | 181 | 186 | 223 | 56 | 17 | 266 | 362 | 330 | 91 | 209 | 229 | |
Labelled Cell Count | 243 | 308 | 297 | 206 | 236 | 267 | 304 | 216 | 352 | 365 | 384 | 213 | 189 | 188 | 228 | 61 | 20 | 266 | 362 | 330 | 91 | 209 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 4.6 | 5.6 | 5.2 | 5.4 | 4.2 | 4.3 | 4.5 | 3.6 | 3.6 | 3.4 | 4.0 | 4.9 | 5.0 | 4.7 | 6.0 | – | 6.4 | 4.5 | 4.7 | 4.9 |
Std Deviation (1e-4) | 0.6 | 0.6 | 0.6 | 1.1 | 1.3 | 0.8 | 0.8 | 0.8 | 0.5 | 0.7 | 0.5 | 0.8 | 0.9 | 1.5 | 1.1 | 1.5 | – | 1.4 | 0.7 | 0.9 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.04 | -0.43 | -0.36 | -0.31 | -0.64 | -0.62 | -0.7 | -0.47 | -0.2 | -0.15 | -0.24 | 0.11 | – | 0.19 | -0.32 | -0.26 | -0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.2948 | 0.4701 | 0.4262 | -0.1393 | 0.1787 | 0.1641 | 0.9893 | 1.2598 | 1.2035 | 0.5987 | 1.2196 | 1.1471 | 0.4427 | 0.2474 | 0.4053 | 0.6111 | -0.0092 | -0.1799 |
Actin | 0.0718 | 0.016 | 0.0304 | 0.0074 | 0.0101 | 0.0048 | 0.0401 | 0.0132 | 0.0203 | 0.0022 | 0.0511 | 0.008 | 0.0285 | 0.0074 | 0.0127 | 0.0107 | 0.003 | 0.0093 |
Bud | 0.0017 | 0.0039 | 0.0017 | 0.0034 | 0.0061 | 0.0009 | 0.0028 | 0.007 | 0.0069 | 0.0039 | 0.0032 | 0.003 | 0.0022 | 0.0027 | 0.0023 | 0.002 | 0.0014 | 0.0008 |
Bud Neck | 0.0089 | 0.0026 | 0.007 | 0.0011 | 0.004 | 0.0267 | 0.0037 | 0.0016 | 0.008 | 0.0011 | 0.0044 | 0.0102 | 0.0121 | 0.0016 | 0.0013 | 0.0058 | 0.006 | 0.0126 |
Bud Periphery | 0.0035 | 0.0072 | 0.0031 | 0.0089 | 0.0188 | 0.0017 | 0.0069 | 0.0113 | 0.0128 | 0.0104 | 0.0043 | 0.0042 | 0.0041 | 0.0059 | 0.0028 | 0.0032 | 0.005 | 0.0017 |
Bud Site | 0.0196 | 0.0363 | 0.0081 | 0.003 | 0.0032 | 0.0009 | 0.0113 | 0.0494 | 0.0256 | 0.0033 | 0.0175 | 0.0025 | 0.0099 | 0.0368 | 0.0264 | 0.0053 | 0.0082 | 0.0009 |
Cell Periphery | 0.0033 | 0.0027 | 0.003 | 0.0015 | 0.0005 | 0.0007 | 0.0015 | 0.0016 | 0.0011 | 0.0012 | 0.0005 | 0.0007 | 0.0016 | 0.0017 | 0.0012 | 0.0014 | 0.003 | 0.0004 |
Cytoplasm | 0.1442 | 0.2837 | 0.3025 | 0.3436 | 0.1046 | 0.3987 | 0.1624 | 0.2369 | 0.2187 | 0.1841 | 0.1338 | 0.218 | 0.1973 | 0.2873 | 0.2227 | 0.301 | 0.3143 | 0.248 |
Cytoplasmic Foci | 0.1287 | 0.1331 | 0.1182 | 0.1261 | 0.2205 | 0.115 | 0.1688 | 0.1817 | 0.1674 | 0.1506 | 0.1796 | 0.1989 | 0.1084 | 0.1123 | 0.1093 | 0.1455 | 0.2169 | 0.1623 |
Eisosomes | 0.0008 | 0.0008 | 0.0006 | 0.0003 | 0.0002 | 0.0004 | 0.0008 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0005 | 0.0004 | 0.0006 | 0.0002 | 0.0003 |
Endoplasmic Reticulum | 0.0107 | 0.0104 | 0.0094 | 0.0109 | 0.0168 | 0.007 | 0.0083 | 0.006 | 0.0042 | 0.0047 | 0.0135 | 0.0036 | 0.0169 | 0.0111 | 0.0114 | 0.0065 | 0.0033 | 0.0076 |
Endosome | 0.1854 | 0.1433 | 0.1478 | 0.2212 | 0.1897 | 0.1179 | 0.2042 | 0.1625 | 0.1591 | 0.2344 | 0.1831 | 0.1709 | 0.176 | 0.1398 | 0.1839 | 0.212 | 0.2426 | 0.1738 |
Golgi | 0.0299 | 0.0296 | 0.0525 | 0.0328 | 0.0613 | 0.0293 | 0.0621 | 0.0505 | 0.0432 | 0.0587 | 0.0724 | 0.0625 | 0.0286 | 0.0315 | 0.0365 | 0.0658 | 0.0133 | 0.0462 |
Lipid Particles | 0.0243 | 0.0115 | 0.0236 | 0.0102 | 0.0056 | 0.0152 | 0.019 | 0.0072 | 0.0091 | 0.0346 | 0.0115 | 0.0089 | 0.0233 | 0.0092 | 0.0096 | 0.011 | 0.0011 | 0.0216 |
Mitochondria | 0.0378 | 0.023 | 0.0489 | 0.094 | 0.081 | 0.04 | 0.0999 | 0.084 | 0.1099 | 0.1513 | 0.134 | 0.1127 | 0.0533 | 0.0374 | 0.0806 | 0.1136 | 0.0037 | 0.0551 |
None | 0.2561 | 0.2606 | 0.1989 | 0.1029 | 0.2288 | 0.2164 | 0.1491 | 0.1369 | 0.1384 | 0.0983 | 0.114 | 0.1335 | 0.2238 | 0.2742 | 0.2167 | 0.0737 | 0.1493 | 0.224 |
Nuclear Periphery | 0.0099 | 0.0031 | 0.0029 | 0.0036 | 0.0048 | 0.0008 | 0.0046 | 0.0024 | 0.005 | 0.0013 | 0.0116 | 0.0009 | 0.02 | 0.0016 | 0.0059 | 0.0017 | 0.0005 | 0.0012 |
Nucleolus | 0.0029 | 0.001 | 0.0007 | 0.0008 | 0.0004 | 0.0003 | 0.0007 | 0.0015 | 0.0013 | 0.0005 | 0.0009 | 0.0005 | 0.016 | 0.0018 | 0.0035 | 0.0008 | 0.0001 | 0.0003 |
Nucleus | 0.0191 | 0.0023 | 0.0054 | 0.003 | 0.0015 | 0.0012 | 0.0067 | 0.0028 | 0.009 | 0.0021 | 0.0032 | 0.0026 | 0.0111 | 0.0026 | 0.0274 | 0.0017 | 0.0007 | 0.0009 |
Peroxisomes | 0.0133 | 0.0112 | 0.0201 | 0.0093 | 0.0186 | 0.0125 | 0.0278 | 0.0227 | 0.0377 | 0.0399 | 0.043 | 0.0455 | 0.0209 | 0.0156 | 0.0196 | 0.011 | 0.0195 | 0.0234 |
Punctate Nuclear | 0.0037 | 0.002 | 0.0028 | 0.0022 | 0.0012 | 0.0013 | 0.0027 | 0.0018 | 0.0058 | 0.0013 | 0.0037 | 0.0036 | 0.0155 | 0.0036 | 0.0089 | 0.0007 | 0.0005 | 0.0017 |
Vacuole | 0.0192 | 0.0129 | 0.01 | 0.0105 | 0.0155 | 0.0066 | 0.0127 | 0.0143 | 0.0123 | 0.0118 | 0.009 | 0.0065 | 0.023 | 0.0123 | 0.0131 | 0.0212 | 0.0052 | 0.0054 |
Vacuole Periphery | 0.0054 | 0.0027 | 0.0024 | 0.0031 | 0.0071 | 0.002 | 0.0039 | 0.0043 | 0.0037 | 0.0042 | 0.0052 | 0.0026 | 0.0071 | 0.0031 | 0.0038 | 0.0048 | 0.002 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.1083 | 25.6027 | 12.3322 | 23.2028 | 22.8012 | 18.0786 | 19.8693 | 17.1653 | 12.8002 | 24.6297 |
Translational Efficiency | 0.7273 | 0.5452 | 0.9248 | 0.2951 | 0.5616 | 0.7134 | 0.4993 | 0.4433 | 0.7351 | 0.6902 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|