Standard name
Human Ortholog
Description Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.05 0 0 0 0 0 0 0.06 0.06 0.06 0.1 0.11 0.07 0.08 0.09 0.11 0.1 0.09 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.99 0.98 0.96 0.94 0.87 0.83 0.88 0.81 0.76 0.76 0.98 0.98 0.98 0.96 0.98 0.98 0.93 0.9 0.96 0.92 0.91 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0.07 0 0 0.07 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0.07 0.12 0.11 0.19 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 3 2 3 3 4 0 1 0 0 0 0 5 2 0 0 0 1
Bud 4 2 12 0 1 1 2 7 2 3 5 2 2 0 0 0 0 2 26 12 2 3 7 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 41 13 2 5 17 13 18 25 25 28 32 44 14 22 26 40 26 19 1 1 0 0 0 0
Cytoplasm 818 315 285 299 389 288 468 344 382 380 237 316 200 288 298 349 251 214 769 284 273 193 241 233
Endoplasmic Reticulum 6 0 1 2 13 3 35 14 12 31 12 42 1 1 0 12 3 5 3 1 2 0 2 2
Endosome 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 2 2 0 1 1 2
Golgi 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 0 0 3
Mitochondria 3 3 2 8 4 15 25 30 51 51 61 66 1 0 1 0 1 0 0 1 2 1 0 0
Nucleus 0 2 0 0 0 4 3 8 0 1 2 3 0 1 1 0 0 6 0 1 0 2 0 0
Nuclear Periphery 1 0 0 0 0 1 3 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
Nucleolus 0 0 0 0 0 1 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 2 3 0 0 3 1 7 22 4 15 2 2 1 1 0 11 4 5 2 2 0 2 7 5
Unique Cell Count 827 324 288 304 407 307 537 414 434 469 313 415 205 293 304 364 255 219 825 316 286 210 266 270
Labelled Cell Count 877 342 303 314 427 327 561 454 479 512 354 480 220 316 326 413 286 251 825 316 286 210 266 270


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.2 19.8 8.5 12.9 13.6 11.7 11.7 10.9 10.5 10.7 9.5 9.9 30.7 28.9 26.8 17.2 15.2 14.7 16.6 17.0 18.5
Std Deviation (1e-4) 5.2 6.7 2.5 4.3 4.0 3.5 3.5 3.7 3.3 3.2 2.8 2.9 12.2 10.3 10.6 6.8 5.1 5.9 7.4 5.4 7.3
Intensity Change (Log2) – – – 0.6 0.68 0.46 0.46 0.35 0.3 0.33 0.17 0.22 1.85 1.76 1.66 1.01 0.84 0.79 0.97 1.0 1.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud -3.6 -3.8 -3.2 0 -2.0 -3.5 -3.4 -1.9 -3.4 -2.1 -3.5 -3.6 -3.9 -3.3 -2.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.8 2.8 2.4 3.6 3.5 3.6 5.1 5.2 3.8 4.1 4.5 5.3 5.0 4.5
Cytoplasm -0.6 -2.6 -3.3 -5.7 -6.8 -5.4 -7.3 -8.4 -8.4 -1.2 -0.7 -0.9 -2.4 -0.5 -1.1
Endoplasmic Reticulum 0 2.6 0 4.1 2.7 2.4 4.2 2.9 5.3 0 0 0 2.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 3.0 4.1 5.6 5.3 7.5 6.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.0 0 3.1 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.19 9.4221 8.414 7.6308 8.6868 8.5012 8.3454 11.6846 9.4741 7.3611 10.5422 10.0994 6.3098 7.7692 6.2705 6.5653 6.3213 7.6706
Actin 0.011 0.003 0.0019 0.0038 0.0008 0.0033 0.0138 0.0021 0.0056 0.0057 0.0022 0.0017 0.0226 0.0008 0.0191 0.0071 0.0372 0.0038
Bud 0.0009 0.0144 0.0037 0.0003 0.0086 0.002 0.001 0.0046 0.0034 0.0121 0.0005 0.0006 0.0003 0.0016 0.0014 0.0002 0.001 0.0001
Bud Neck 0.0013 0.0006 0.0004 0.0005 0.0005 0.0013 0.004 0.0004 0.0028 0.001 0.0013 0.0015 0.0009 0.0002 0.0004 0.0003 0.0012 0.0011
Bud Periphery 0.0011 0.0045 0.0025 0.0001 0.0179 0.0013 0.0008 0.0015 0.0029 0.0179 0.0005 0.0005 0.0004 0.0007 0.0008 0 0.001 0.0001
Bud Site 0.0033 0.0165 0.0026 0.0008 0.0027 0.0002 0.0051 0.0145 0.0085 0.0094 0.0013 0.0001 0.0015 0.01 0.0078 0.0006 0.0046 0.0001
Cell Periphery 0.0003 0.0005 0.0002 0.0001 0.0014 0.0001 0.0003 0.0003 0.0005 0.0008 0.0001 0.0001 0.0002 0.0003 0.0003 0 0.0001 0
Cytoplasm 0.9077 0.9258 0.9631 0.9372 0.9381 0.9604 0.8444 0.9554 0.9133 0.8844 0.8383 0.9701 0.8815 0.9703 0.929 0.8735 0.7747 0.9597
Cytoplasmic Foci 0.0089 0.0033 0.0035 0.0144 0.0038 0.0049 0.0259 0.0023 0.0154 0.0093 0.0423 0.0029 0.014 0.0014 0.0065 0.0709 0.0492 0.0073
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0.0001 0.0001 0 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0095 0.0022 0.0035 0.0066 0.0054 0.0032 0.0068 0.0009 0.0013 0.0037 0.0027 0.0032 0.0061 0.001 0.0032 0.0028 0.0053 0.0032
Endosome 0.0167 0.0013 0.0013 0.014 0.0015 0.0042 0.0318 0.0005 0.0065 0.0156 0.0408 0.0036 0.0157 0.0003 0.0064 0.0021 0.0226 0.0113
Golgi 0.0041 0.0003 0.0002 0.0022 0.0001 0.0006 0.0067 0.0002 0.0029 0.0022 0.0125 0.0024 0.0058 0.0001 0.0047 0.0015 0.0149 0.0037
Lipid Particles 0.0019 0 0.0001 0.0032 0.0001 0.0007 0.0071 0 0.0058 0.011 0.0213 0.0021 0.0089 0 0.0005 0.0002 0.0239 0.0014
Mitochondria 0.0018 0.0003 0.0004 0.0004 0.0002 0.0004 0.0042 0.0001 0.0032 0.0013 0.0013 0.0014 0.0055 0 0.0013 0.0003 0.0078 0.0005
None 0.014 0.0198 0.011 0.0072 0.0079 0.0107 0.0082 0.0132 0.004 0.0124 0.001 0.0061 0.0106 0.0112 0.0134 0.0026 0.004 0.0029
Nuclear Periphery 0.0075 0.001 0.001 0.0023 0.0017 0.0019 0.0061 0.0004 0.0008 0.002 0.0036 0.0005 0.0054 0.0003 0.0014 0.0012 0.0119 0.0008
Nucleolus 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0033 0 0.0002 0.0004 0.0005 0 0.0003 0 0 0 0.0005 0
Nucleus 0.0038 0.0035 0.0024 0.0023 0.0037 0.002 0.0044 0.0023 0.0022 0.0038 0.0061 0.0015 0.0023 0.001 0.0012 0.0015 0.0021 0.0013
Peroxisomes 0.0009 0.0001 0.0001 0.0005 0.0001 0.0003 0.0063 0.0001 0.0169 0.0012 0.0123 0.0004 0.006 0 0.001 0.0297 0.0183 0.0009
Punctate Nuclear 0.0011 0.0005 0.0006 0.0026 0.0006 0.0016 0.011 0.0006 0.002 0.0022 0.0021 0.0002 0.0091 0.0001 0.0006 0.0049 0.0172 0.0005
Vacuole 0.0031 0.0019 0.0012 0.001 0.0046 0.0009 0.0071 0.0005 0.0014 0.003 0.0083 0.0007 0.0016 0.0005 0.0007 0.0004 0.0013 0.0009
Vacuole Periphery 0.0006 0.0002 0.0002 0.0002 0.0004 0.0002 0.0016 0 0.0002 0.0006 0.0011 0.0002 0.0009 0 0.0002 0.0001 0.0009 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.4879 90.7708 121.2405 167.4485 138.9654 33.828 79.5412 81.9039 110.6553 117.5649
Translational Efficiency 2.9069 2.5078 2.4351 2.1027 2.7984 3.7496 2.3956 2.337 2.3763 2.4563

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phospholipid hydroperoxide glutathione peroxidase; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Gpx2

Gpx2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpx2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available