Standard name | |
---|---|
Human Ortholog | |
Description | Imidazole glycerol phosphate synthase; glutamine amidotransferase:cyclase that catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | – | 10 | 4 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | – | 0 | 2 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 3 | 7 | 9 | 6 | – | 16 | 29 | 32 | 0 | 2 | 1 | 7 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 257 | 158 | 275 | 337 | 418 | 435 | 535 | 421 | – | 463 | 462 | 514 | 111 | 218 | 241 | 354 | 343 | 323 | 236 | 146 | 260 | 87 | 176 | 166 |
Endoplasmic Reticulum | 0 | 1 | 0 | 2 | 4 | 18 | 48 | 58 | – | 47 | 32 | 42 | 1 | 3 | 0 | 13 | 11 | 10 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 1 | 0 | 0 | 3 | 1 | 3 | – | 4 | 18 | 25 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | – | 4 | 2 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 0 | 1 | 3 | 13 | 18 | – | 27 | 34 | 27 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 3 | 2 | 5 |
Unique Cell Count | 258 | 159 | 276 | 339 | 422 | 460 | 605 | 505 | 554 | 554 | 633 | 111 | 224 | 241 | 359 | 343 | 325 | 246 | 155 | 266 | 100 | 192 | 189 | |
Labelled Cell Count | 260 | 161 | 276 | 341 | 426 | 468 | 614 | 514 | 573 | 585 | 668 | 112 | 225 | 243 | 377 | 360 | 341 | 246 | 155 | 266 | 100 | 192 | 189 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 84.3 | 90.9 | 56.7 | 50.9 | 39.0 | 30.3 | 27.4 | 24.9 | – | 22.9 | 22.5 | 21.2 | 59.6 | 65.2 | 60.1 | 94.5 | 95.0 | 92.7 | 63.6 | 68.6 | 70.6 |
Std Deviation (1e-4) | 26.6 | 21.8 | 11.5 | 10.3 | 7.9 | 6.5 | 6.1 | 6.3 | – | 5.4 | 5.7 | 5.3 | 16.7 | 17.9 | 16.0 | 23.4 | 20.7 | 21.5 | 23.1 | 20.3 | 27.5 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.54 | -0.9 | -1.05 | -1.19 | – | -1.31 | -1.34 | -1.42 | 0.07 | 0.2 | 0.08 | 0.74 | 0.74 | 0.71 | 0.17 | 0.27 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 2.9 | 3.9 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -0.9 | -3.6 | -5.7 | -7.0 | – | -6.9 | -7.0 | -7.6 | 0.6 | -2.2 | 0.9 | -1.3 | 1.1 | -0.4 |
Endoplasmic Reticulum | 0 | 0 | 3.3 | 4.8 | 5.9 | – | 5.0 | 4.1 | 4.4 | 0 | 0 | 0 | 3.2 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.6 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.2 | – | 3.7 | 4.2 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 64.8588 | 75.0759 | 70.963 | 61.1651 | 65.9355 | 73.1957 | 62.4792 | 72.3154 | 68.2364 | 62.1367 | 60.2968 | 67.5566 | 46.8239 | 77.1638 | 66.9521 | 71.821 | 49.6222 | 90.962 |
Actin | 0.0013 | 0 | 0.0001 | 0.0063 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0002 | 0.0164 | 0.0001 | 0.0007 | 0.0028 | 0.0026 | 0 |
Bud | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0018 | 0 | 0.0002 | 0.0004 | 0.0055 | 0 |
Bud Neck | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0006 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0001 |
Bud Periphery | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0031 | 0 | 0 | 0.0002 | 0.0068 | 0 |
Bud Site | 0.0002 | 0 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 | 0 | 0 | 0.001 | 0 | 0.0001 | 0 | 0.0005 | 0 |
Cell Periphery | 0.0007 | 0.0012 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0.0003 | 0 |
Cytoplasm | 0.9441 | 0.9916 | 0.9858 | 0.9563 | 0.9843 | 0.982 | 0.9749 | 0.9967 | 0.9903 | 0.9808 | 0.9941 | 0.9885 | 0.8154 | 0.9982 | 0.9912 | 0.9556 | 0.6885 | 0.9969 |
Cytoplasmic Foci | 0.0029 | 0.0001 | 0.0002 | 0.0018 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0016 | 0.0043 | 0.0001 | 0.0003 | 0.0046 | 0.0001 | 0.0019 | 0.0003 | 0.0027 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0014 | 0.0005 | 0.0002 | 0.002 | 0.0003 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0346 | 0 | 0.0006 | 0.007 | 0.0263 | 0 |
Endosome | 0.0012 | 0 | 0.0001 | 0.0049 | 0 | 0 | 0.0003 | 0 | 0.0017 | 0.0004 | 0 | 0.0002 | 0.0139 | 0 | 0.0007 | 0.0019 | 0.1459 | 0 |
Golgi | 0.0004 | 0 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0 | 0 | 0.0021 | 0 | 0.0001 | 0.0001 | 0.0226 | 0 |
Lipid Particles | 0.0016 | 0.0001 | 0 | 0.0008 | 0 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Mitochondria | 0.0016 | 0 | 0 | 0.0002 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0124 | 0 | 0.0004 | 0.0005 | 0.0233 | 0 |
None | 0.0021 | 0.0001 | 0.0002 | 0.0017 | 0.0001 | 0.0001 | 0.0016 | 0.0001 | 0.0002 | 0.0014 | 0.0001 | 0.0001 | 0.005 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0.0001 |
Nuclear Periphery | 0.0057 | 0.0009 | 0.0014 | 0.0044 | 0.0016 | 0.0027 | 0.0036 | 0.0003 | 0.0004 | 0.0015 | 0.0009 | 0.0018 | 0.0528 | 0.0001 | 0.0005 | 0.0192 | 0.0095 | 0.0002 |
Nucleolus | 0.0053 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0246 | 0.0042 | 0.0098 | 0.012 | 0.0121 | 0.0131 | 0.0149 | 0.0023 | 0.0032 | 0.0091 | 0.0043 | 0.0076 | 0.0169 | 0.0012 | 0.0019 | 0.0093 | 0.0028 | 0.0025 |
Peroxisomes | 0.0003 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Punctate Nuclear | 0.0006 | 0.0001 | 0.0001 | 0.0043 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0026 | 0 | 0.0002 | 0.0003 | 0.0001 | 0 |
Vacuole | 0.0037 | 0.001 | 0.0014 | 0.0015 | 0.0005 | 0.0005 | 0.0007 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0061 | 0 | 0.0004 | 0.0009 | 0.0268 | 0 |
Vacuole Periphery | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0096 | 0 | 0.0001 | 0.0007 | 0.035 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 49.2176 | 46.3757 | 34.4128 | 40.7519 | 42.2544 | 46.5314 | 45.8384 | 48.6891 | 45.8534 | 43.8466 |
Translational Efficiency | 1.4965 | 1.2451 | 1.5128 | 1.5741 | 1.4438 | 1.4282 | 1.4893 | 1.1965 | 1.225 | 1.2146 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|