Standard name
Human Ortholog
Description 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.97 0.97 0.92 0.89 0.79 0.59 0.37 0.39 0.3 0.37 0.17 0.61 0.96 0.99 0.98 0.99 0.55 0.49 0.71 0.51 0.65 0.72
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.06 0.07 0.08 0 0.07 0 0 0 0 0 0.06 0 0.05 0.05 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.07 0.09 0.18 0.18 0.12 0.14 0.81 0.2 0 0 0 0 0.24 0.31 0.14 0.33 0.17 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.07 0.06 0.09 0.06 0.05 0.09 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.09 0.19 0.28 0.3 0.43 0.39 0 0.07 0 0 0 0 0 0.07 0 0.08 0.06 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 2 3 1 3 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 0 0 0 0 1 0 1 2 1 0 0 0 1 0 1 0 1 2 0 1 6 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3 1 1 2 2 0 0 0 0 0 1 0 2 0 0 0 0 1 0
Cytoplasm 109 17 88 125 128 139 142 86 66 56 27 37 25 69 135 229 162 202 51 46 108 65 160 180
Endoplasmic Reticulum 0 0 1 0 6 8 11 10 15 5 6 3 0 2 3 8 10 9 4 4 5 1 4 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 1 0 0 2 0 6 4 4 0 3 0 2 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2 1 2 2 0 4 0 0 1 0 0 0 0 0 0 0 0
Nucleus 1 0 1 2 1 1 13 13 32 26 11 14 118 23 0 0 0 0 22 29 21 41 42 27
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0
Nucleolus 2 0 0 1 0 1 1 5 12 9 8 6 8 10 1 0 0 0 0 0 0 0 2 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Vac/Vac Membrane 0 0 1 2 5 6 16 28 49 42 39 39 2 8 2 0 0 0 2 7 5 10 15 15
Unique Cell Count 111 17 91 129 139 156 179 147 178 142 91 99 146 113 140 231 165 204 95 95 152 128 247 250
Labelled Cell Count 112 17 92 130 140 160 185 152 184 151 95 105 157 114 142 239 173 213 95 95 152 128 247 250


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 152.3 198.7 136.6 130.0 104.8 78.9 58.9 54.3 47.3 44.3 38.3 41.7 152.8 146.1 146.9 211.8 227.9 219.0 137.8 145.8 150.7
Std Deviation (1e-4) 39.8 57.6 30.3 32.0 26.8 19.0 14.8 14.8 10.6 11.8 12.5 13.2 48.8 46.5 45.2 58.8 71.7 68.3 43.4 40.5 42.6
Intensity Change (Log2) -0.07 -0.38 -0.79 -1.21 -1.33 -1.53 -1.62 -1.83 -1.71 0.16 0.1 0.1 0.63 0.74 0.68 0.01 0.09 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 -1.4 -2.1 -3.8 -6.5 -9.4 -8.8 -9.4 -8.6 -11.9 -6.0 -0.1 1.6 0.7 1.4
Endoplasmic Reticulum 0 0 0 0 0 2.4 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 2.5 4.0 4.0 3.0 3.3 11.9 4.2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 4.1 5.3 5.5 6.8 6.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 60.873 209.0777 205.8403 166.4663 115.1939 103.7642 135.3797 174.5884 167.2258 149.1026 121.9713 150.5156 127.3028 173.0921 157.8855 140.165 109.1312 148.7444
Actin 0.0211 0.0026 0.009 0.0044 0.0244 0.048 0.0003 0.0002 0.0002 0.0002 0.001 0.0001 0.0101 0.0002 0.0001 0.0532 0.0049 0.0002
Bud 0.0124 0.0009 0.0001 0.004 0.0008 0.0055 0.0007 0.0005 0.0002 0.0002 0.0028 0.0001 0.0017 0.0008 0.0002 0.001 0.005 0.0003
Bud Neck 0.0012 0.0003 0.0008 0.0004 0.0004 0.0018 0 0.0001 0.0002 0.0001 0.0004 0.0004 0.0002 0.0001 0.0001 0.0011 0.0004 0.0005
Bud Periphery 0.006 0.0003 0 0.006 0.0005 0.005 0.0001 0.0001 0 0 0.0009 0 0.0007 0.0002 0.0001 0.0006 0.0017 0.0001
Bud Site 0.0082 0.0006 0.0001 0.0037 0.0003 0.0093 0 0.0001 0 0 0.001 0 0.0008 0.0002 0.0001 0.0044 0.0009 0
Cell Periphery 0.0068 0.0001 0 0.0004 0.0006 0.0009 0 0.0001 0 0 0.0019 0 0.0008 0.0006 0.0001 0.0001 0.001 0.0001
Cytoplasm 0.2752 0.794 0.6386 0.5533 0.4622 0.4255 0.222 0.6145 0.5401 0.6438 0.3945 0.2951 0.3451 0.6196 0.4687 0.447 0.3872 0.6784
Cytoplasmic Foci 0.1261 0.001 0.002 0.0021 0.0453 0.0402 0 0.0001 0.0003 0.0009 0.0567 0 0.0314 0.0034 0.0108 0.0195 0.0109 0.0003
Eisosomes 0.0012 0 0 0.0001 0.001 0.0002 0 0 0 0 0.0003 0 0.0002 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0045 0.0001 0.0001 0.0035 0.0041 0.0141 0.0001 0.0001 0.0001 0.0006 0.0013 0.0002 0.0009 0.0001 0.0001 0.0003 0.0011 0.0006
Endosome 0.0507 0.0001 0.0008 0.0076 0.0161 0.1228 0 0 0.0001 0.0022 0.0015 0 0.0035 0.0012 0.0001 0.0031 0.0571 0.0003
Golgi 0.0233 0.0003 0.0026 0.0024 0.0184 0.0356 0 0 0 0.0001 0.0003 0 0.0015 0.0012 0 0.0094 0.0453 0
Lipid Particles 0.0319 0.0002 0.0003 0.0003 0.0106 0.0104 0 0 0 0 0.0067 0 0.0079 0.0002 0.0011 0.0005 0.0048 0
Mitochondria 0.0268 0.0011 0.0005 0.0548 0.0553 0.0205 0.0045 0.0006 0.0005 0.0006 0.0099 0.0002 0.0092 0.0006 0.0024 0.0014 0.0967 0.0003
None 0.0326 0.0012 0.0003 0.0019 0.0405 0.0176 0.0002 0.0002 0.0004 0.0002 0.0391 0 0.0118 0.0012 0.0036 0.0002 0.0093 0.0003
Nuclear Periphery 0.0166 0.0104 0.0212 0.0161 0.0358 0.0445 0.0219 0.0155 0.0186 0.0137 0.0445 0.0403 0.0491 0.0214 0.0326 0.032 0.0347 0.0427
Nucleolus 0.1003 0.0034 0.0006 0.0004 0.0192 0.0117 0.0005 0.0007 0.0024 0.0001 0.0385 0.0001 0.0233 0.0016 0.0069 0.0002 0.0021 0.0015
Nucleus 0.1836 0.1806 0.3187 0.3331 0.2522 0.1619 0.7483 0.3659 0.4341 0.3353 0.3713 0.6625 0.4851 0.3445 0.4684 0.417 0.3245 0.2687
Peroxisomes 0.0161 0.0004 0.0012 0.0006 0.0023 0.0039 0 0 0 0 0.0008 0 0.001 0.0003 0.0002 0.008 0.0022 0
Punctate Nuclear 0.0254 0.0005 0.0028 0.0005 0.0017 0.0074 0.0002 0.0003 0.0014 0.0002 0.0056 0.0003 0.007 0.001 0.0016 0.0007 0.0006 0.0006
Vacuole 0.0223 0.0014 0.0002 0.003 0.0031 0.0082 0.0003 0.0007 0.0007 0.0009 0.0135 0.0004 0.0047 0.0013 0.0013 0.0002 0.007 0.0031
Vacuole Periphery 0.0077 0.0007 0.0002 0.0015 0.0052 0.0049 0.0007 0.0003 0.0006 0.0009 0.0074 0.0004 0.0041 0.0003 0.0014 0.0002 0.0025 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 436.2066 410.3254 372.8719 333.0201 335.4805 457.7634 413.5355 358.6379 344.4213 356.2968
Translational Efficiency 1.7295 1.8796 1.8095 1.8158 2.1107 2.0059 1.815 1.9773 1.8784 1.9757

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Aro4

Aro4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aro4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available