Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 13 | 10 | 10 | 11 | 11 | 5 | 9 | 7 | 5 | 3 | 2 | 1 | 15 | 5 | 7 | 32 | 22 | 13 | 37 | 21 | 50 | 54 | 87 | 81 |
Bud | 5 | 7 | 4 | 3 | 3 | 3 | 9 | 8 | 27 | 26 | 23 | 18 | 10 | 8 | 6 | 8 | 1 | 1 | 0 | 0 | 2 | 3 | 3 | 3 |
Bud Neck | 0 | 4 | 16 | 14 | 30 | 16 | 14 | 15 | 13 | 8 | 3 | 4 | 0 | 2 | 1 | 2 | 1 | 1 | 5 | 6 | 24 | 19 | 18 | 31 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 3 | 7 | 42 | 14 | 25 | 20 | 18 | 11 | 8 | 5 | 3 | 6 | 13 | 9 | 15 | 0 | 1 | 0 | 10 | 17 | 82 | 38 | 46 | 69 |
Cytoplasm | 35 | 99 | 319 | 287 | 437 | 440 | 571 | 527 | 427 | 350 | 365 | 329 | 182 | 323 | 366 | 36 | 21 | 17 | 7 | 45 | 86 | 32 | 38 | 59 |
Endoplasmic Reticulum | 21 | 14 | 11 | 2 | 5 | 5 | 6 | 8 | 0 | 3 | 0 | 1 | 14 | 5 | 22 | 21 | 6 | 6 | 34 | 30 | 42 | 36 | 56 | 55 |
Endosome | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 34 | 20 | 88 | 33 | 35 | 5 | 10 | 7 | 3 | 10 | 14 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 10 | 9 | 1 | 3 | 1 | 2 | 2 | 4 |
Mitochondria | 12 | 16 | 0 | 3 | 6 | 42 | 61 | 31 | 166 | 148 | 199 | 166 | 9 | 3 | 3 | 23 | 20 | 11 | 14 | 41 | 89 | 50 | 89 | 69 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 7 | 2 | 3 | 7 | 4 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 29 | 7 | 25 | 2 | 1 | 2 | 1 | 0 | 2 | 6 | 4 |
SpindlePole | 2 | 3 | 4 | 7 | 3 | 3 | 6 | 7 | 6 | 9 | 9 | 14 | 4 | 25 | 12 | 74 | 37 | 35 | 0 | 2 | 1 | 0 | 2 | 2 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 6 | 7 | 7 | 0 | 1 | 0 | 10 | 7 | 2 | 5 | 18 | 23 | 13 | 13 | 16 |
Unique Cell Count | 78 | 147 | 356 | 311 | 469 | 489 | 627 | 587 | 566 | 493 | 544 | 487 | 223 | 398 | 427 | 255 | 131 | 111 | 128 | 205 | 423 | 261 | 381 | 419 |
Labelled Cell Count | 93 | 164 | 406 | 341 | 522 | 538 | 696 | 623 | 663 | 564 | 620 | 553 | 251 | 446 | 459 | 326 | 166 | 136 | 128 | 205 | 423 | 261 | 381 | 419 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.6 | 4.6 | 5.1 | 4.5 | 5.0 | 4.0 | 4.2 | 4.6 | 3.6 | 3.6 | 3.4 | 4.0 | 4.4 | 4.8 | 4.7 | 7.2 | 7.6 | 7.2 | 4.5 | 4.3 | 4.7 |
Std Deviation (1e-4) | 0.6 | 1.4 | 0.7 | 0.8 | 0.9 | 0.9 | 0.9 | 0.9 | 0.7 | 0.8 | 0.5 | 0.8 | 0.6 | 1.7 | 1.0 | 1.4 | 1.8 | 1.3 | 2.2 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.18 | -0.03 | -0.34 | -0.3 | -0.17 | -0.52 | -0.49 | -0.58 | -0.35 | -0.22 | -0.1 | -0.11 | 0.49 | 0.58 | 0.5 | -0.18 | -0.26 | -0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.358 | -0.2881 | -0.372 | -0.039 | -0.2532 | -0.3724 | -0.2059 | -0.3089 | -0.3829 | -0.6213 | -0.993 | -0.2764 | -0.5199 | 0.0048 | -0.2545 | 0.0208 | -0.6373 | -0.3835 |
Actin | 0.0189 | 0.0166 | 0.018 | 0.0287 | 0.0086 | 0.0116 | 0.0663 | 0.0266 | 0.0453 | 0.0438 | 0.0028 | 0.0197 | 0.02 | 0.0147 | 0.0355 | 0.0063 | 0.0178 | 0.0154 |
Bud | 0.0007 | 0.0009 | 0.0007 | 0.0015 | 0.0007 | 0.0002 | 0.001 | 0.0078 | 0.0017 | 0.0004 | 0.0002 | 0.0021 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0001 | 0.0002 |
Bud Neck | 0.008 | 0.003 | 0.0089 | 0.0013 | 0.0036 | 0.0087 | 0.0056 | 0.0035 | 0.0095 | 0.0079 | 0.0028 | 0.0196 | 0.0036 | 0.005 | 0.004 | 0.0062 | 0.001 | 0.0058 |
Bud Periphery | 0.0015 | 0.0015 | 0.0015 | 0.015 | 0.0013 | 0.0004 | 0.0019 | 0.004 | 0.0029 | 0.0007 | 0.0005 | 0.0055 | 0.0002 | 0.0007 | 0.0009 | 0.0003 | 0.0001 | 0.0005 |
Bud Site | 0.0082 | 0.0287 | 0.0152 | 0.0011 | 0.0182 | 0.0003 | 0.0173 | 0.0281 | 0.0113 | 0.0007 | 0.0016 | 0.0011 | 0.0012 | 0.0104 | 0.0047 | 0.0014 | 0.0003 | 0.0006 |
Cell Periphery | 0.0021 | 0.0025 | 0.0029 | 0.0006 | 0.0008 | 0.0009 | 0.0021 | 0.0023 | 0.0019 | 0.0028 | 0.0012 | 0.0021 | 0.0009 | 0.0156 | 0.0019 | 0.0005 | 0.0002 | 0.0031 |
Cytoplasm | 0.1059 | 0.1142 | 0.0984 | 0.0367 | 0.081 | 0.2009 | 0.1659 | 0.1446 | 0.1764 | 0.1038 | 0.2865 | 0.2108 | 0.0943 | 0.2449 | 0.1764 | 0.1738 | 0.1098 | 0.2648 |
Cytoplasmic Foci | 0.2188 | 0.2138 | 0.2092 | 0.2131 | 0.2446 | 0.2033 | 0.1864 | 0.2519 | 0.2351 | 0.2972 | 0.1868 | 0.2673 | 0.2351 | 0.1787 | 0.2109 | 0.3591 | 0.2828 | 0.2613 |
Eisosomes | 0.0026 | 0.0012 | 0.0016 | 0.0003 | 0.0006 | 0.0004 | 0.0007 | 0.0004 | 0.0004 | 0.0013 | 0.0002 | 0.0004 | 0.0004 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0096 | 0.0136 | 0.0144 | 0.0041 | 0.012 | 0.0125 | 0.0116 | 0.0101 | 0.0126 | 0.0045 | 0.0039 | 0.0118 | 0.013 | 0.0397 | 0.0227 | 0.0616 | 0.0043 | 0.0148 |
Endosome | 0.1063 | 0.1639 | 0.1384 | 0.169 | 0.1259 | 0.088 | 0.1451 | 0.157 | 0.1654 | 0.1984 | 0.1293 | 0.1121 | 0.1488 | 0.1106 | 0.1397 | 0.1617 | 0.1087 | 0.105 |
Golgi | 0.0178 | 0.02 | 0.0219 | 0.064 | 0.0166 | 0.0157 | 0.0318 | 0.0327 | 0.0334 | 0.0239 | 0.0124 | 0.0305 | 0.0285 | 0.018 | 0.0262 | 0.0135 | 0.0227 | 0.017 |
Lipid Particles | 0.1808 | 0.128 | 0.1283 | 0.1148 | 0.1879 | 0.1166 | 0.152 | 0.1132 | 0.1028 | 0.1121 | 0.1558 | 0.096 | 0.2369 | 0.0931 | 0.1239 | 0.0789 | 0.1508 | 0.0989 |
Mitochondria | 0.0147 | 0.0116 | 0.0073 | 0.046 | 0.0115 | 0.0067 | 0.0057 | 0.0046 | 0.0047 | 0.0024 | 0.0007 | 0.0104 | 0.0031 | 0.0014 | 0.0037 | 0.0011 | 0.0011 | 0.0025 |
None | 0.2295 | 0.2415 | 0.2807 | 0.2376 | 0.1897 | 0.2903 | 0.1172 | 0.1649 | 0.1335 | 0.122 | 0.1563 | 0.1654 | 0.1531 | 0.24 | 0.2093 | 0.0609 | 0.244 | 0.1677 |
Nuclear Periphery | 0.0096 | 0.0021 | 0.0032 | 0.0028 | 0.0096 | 0.0011 | 0.0267 | 0.001 | 0.0063 | 0.0013 | 0.0009 | 0.0013 | 0.0055 | 0.0016 | 0.0018 | 0.0042 | 0.0006 | 0.0019 |
Nucleolus | 0.0011 | 0.001 | 0.0014 | 0.0006 | 0.0107 | 0.0005 | 0.0009 | 0.0011 | 0.0006 | 0.0002 | 0.0005 | 0.0003 | 0.0006 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0004 |
Nucleus | 0.006 | 0.0011 | 0.0022 | 0.0009 | 0.0039 | 0.0011 | 0.0044 | 0.0017 | 0.0032 | 0.0005 | 0.0011 | 0.001 | 0.0031 | 0.0009 | 0.0007 | 0.0005 | 0.0004 | 0.001 |
Peroxisomes | 0.0377 | 0.0208 | 0.0293 | 0.0468 | 0.0369 | 0.0296 | 0.0401 | 0.031 | 0.0398 | 0.0668 | 0.0375 | 0.0301 | 0.026 | 0.0094 | 0.0269 | 0.0593 | 0.0496 | 0.0233 |
Punctate Nuclear | 0.0126 | 0.0038 | 0.0057 | 0.0024 | 0.0278 | 0.0047 | 0.0068 | 0.0041 | 0.0033 | 0.0027 | 0.0161 | 0.0029 | 0.0203 | 0.0032 | 0.0029 | 0.0051 | 0.0036 | 0.0095 |
Vacuole | 0.006 | 0.0093 | 0.0092 | 0.0079 | 0.0062 | 0.0058 | 0.0079 | 0.0087 | 0.0085 | 0.006 | 0.0028 | 0.0085 | 0.0044 | 0.0095 | 0.0057 | 0.0044 | 0.0017 | 0.0053 |
Vacuole Periphery | 0.0014 | 0.0009 | 0.0016 | 0.0046 | 0.002 | 0.0006 | 0.0026 | 0.0007 | 0.0014 | 0.0008 | 0.0003 | 0.001 | 0.0008 | 0.0006 | 0.0007 | 0.0008 | 0.0003 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 3.9691 | 8.0424 | 5.9678 | 8.2015 | 2.7446 | 4.2313 | 7.4142 | 8.3939 | 9.0159 | 8.8908 |
Translational Efficiency | 0.8402 | 0.4743 | 0.6184 | 0.4387 | 1.1291 | 0.8221 | 0.7595 | 0.4469 | 0.5583 | 0.403 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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