Standard name
Human Ortholog
Description AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.98 0.89 0.96 0.87 0.77 0.67 0.73 0.7 0.71 0.96 0.98 0.97 0.95 0.96 0.98 0.91 0.93 0.92 0.9 0.88 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.11 0.1 0.32 0.2 0.28 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.11 0 0 0.25 0.13 0.1 0.05 0.06 0 0 0.09 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 2 1
Bud 0 1 1 0 2 1 3 3 2 0 1 0 0 0 0 0 0 0 0 1 5 8
Bud Neck 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 6 0 0 0 0 1 1 4 1 1 2 1 2 7 4 5 0 0 0 0 0 0
Cytoplasm 283 314 32 65 78 188 123 124 105 137 341 495 489 190 122 152 276 301 58 191 495 385
Endoplasmic Reticulum 2 2 0 1 0 0 2 2 0 4 1 2 1 9 6 5 3 2 0 1 10 4
Endosome 1 0 0 0 0 0 0 3 0 1 2 1 0 0 0 0 8 2 0 1 7 4
Golgi 2 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 5 0 0 0 2 0
Mitochondria 0 1 0 0 10 24 59 34 42 49 0 0 0 1 0 0 3 3 0 0 5 2
Nucleus 1 0 0 1 2 2 2 9 12 15 5 6 2 8 4 2 0 2 1 0 2 2
Nuclear Periphery 0 0 0 0 0 1 2 3 1 0 0 1 0 0 0 0 0 1 0 0 2 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 3 4 4
Vac/Vac Membrane 16 2 4 1 3 61 24 17 7 12 9 9 44 8 4 2 1 3 1 8 12 7
Unique Cell Count 296 319 36 68 90 245 183 169 149 194 354 505 506 199 127 155 305 324 64 213 563 427
Labelled Cell Count 312 320 37 68 97 279 216 199 171 219 362 516 538 223 140 166 305 324 64 213 563 427


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 8.5 7.3 7.9 4.7 6.2 5.3 5.4 5.3 5.2 9.3 8.3 8.9 11.2 11.2 11.9 8.4 8.4 8.9
Std Deviation (1e-4) 2.0 1.5 0.9 1.2 1.1 1.4 1.4 1.2 1.0 0.9 1.8 1.7 1.9 2.1 2.2 2.5 2.0 1.9 2.1
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5037 1.0116 0.6162 0.2101 0.2172 1.0602 2.6129 3.0595 2.8758 2.4186 2.2656 2.8658 4.2347 5.6886 4.9617 4.5651 4.9213 4.9309
Actin 0.0712 0.0005 0.0025 0.0532 0.0697 0.0135 0.0144 0.0016 0.0091 0.0074 0.0217 0.0014 0.0091 0.0002 0.0122 0.0011 0.0009 0.0031
Bud 0.0016 0.0004 0.0002 0.0003 0.0033 0.0005 0.0022 0.0193 0.012 0.0243 0.002 0.0029 0.0002 0.0001 0.0003 0.0002 0.0002 0.0004
Bud Neck 0.0019 0.0001 0.002 0.0006 0.001 0.0006 0.0034 0.0003 0.0017 0.0003 0.0003 0.002 0.0004 0.0001 0.0002 0.0002 0.0002 0.0009
Bud Periphery 0.002 0.0002 0.0001 0.0002 0.0042 0.0003 0.0021 0.0078 0.0041 0.0094 0.0025 0.001 0.0002 0 0.0004 0.0001 0.0001 0.0005
Bud Site 0.0094 0.0008 0.0047 0.002 0.0069 0.0002 0.0148 0.0206 0.0183 0.0018 0.0034 0.0011 0.0014 0.0004 0.0004 0.0003 0.0003 0.0005
Cell Periphery 0.0003 0.0001 0.0002 0.0001 0.0004 0 0.0005 0.0004 0.0003 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.5803 0.9141 0.8342 0.7559 0.4951 0.8941 0.5884 0.7796 0.7237 0.7091 0.6513 0.8803 0.6635 0.7787 0.7157 0.8686 0.7554 0.8149
Cytoplasmic Foci 0.0379 0.0071 0.018 0.0283 0.0254 0.0112 0.0403 0.0117 0.028 0.0457 0.0185 0.0164 0.0109 0.0056 0.0182 0.0114 0.0077 0.007
Eisosomes 0.0003 0 0 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0198 0.0053 0.0143 0.008 0.017 0.0056 0.0062 0.0032 0.0041 0.0072 0.0026 0.0032 0.0081 0.0033 0.0045 0.0071 0.004 0.0021
Endosome 0.0473 0.0074 0.0114 0.0573 0.1092 0.0136 0.0453 0.0104 0.0178 0.016 0.0093 0.0082 0.0082 0.0071 0.0093 0.008 0.0154 0.0036
Golgi 0.0255 0.0005 0.0026 0.0084 0.0435 0.0031 0.0101 0.0034 0.0084 0.0028 0.0072 0.0008 0.0008 0.0002 0.0009 0.0004 0.0008 0.0003
Lipid Particles 0.011 0.0002 0.0009 0.0112 0.0144 0.0019 0.0172 0.0003 0.0026 0.0033 0.0294 0.0001 0.0011 0.0003 0.0009 0.0001 0.001 0.0004
Mitochondria 0.0102 0.0002 0.0003 0.0018 0.0392 0.0019 0.0199 0.0018 0.0156 0.0084 0.0468 0.0013 0.0003 0.0001 0.0004 0.0003 0.0005 0.0002
None 0.1202 0.0566 0.0937 0.0502 0.1059 0.039 0.146 0.1324 0.1238 0.1206 0.1659 0.0636 0.2838 0.1987 0.226 0.0948 0.1935 0.1601
Nuclear Periphery 0.014 0.0017 0.0036 0.0039 0.0288 0.0043 0.0123 0.0009 0.0029 0.0048 0.001 0.0016 0.0029 0.0009 0.0015 0.0017 0.0079 0.0006
Nucleolus 0.0005 0 0.0001 0.001 0.0009 0.0001 0.0044 0.0003 0.001 0.0008 0.0011 0.0002 0.0002 0 0.0001 0 0.0005 0.0001
Nucleus 0.0041 0.0017 0.0021 0.0033 0.0066 0.0051 0.0278 0.0023 0.0144 0.0054 0.0012 0.0077 0.0019 0.0019 0.002 0.0029 0.0048 0.0018
Peroxisomes 0.0157 0.0001 0.0044 0.0033 0.0042 0.0014 0.0107 0.0002 0.0053 0.0078 0.0315 0.0024 0.0002 0 0.0007 0.0001 0.0002 0.0007
Punctate Nuclear 0.0186 0.0004 0.0008 0.0024 0.0032 0.0012 0.0178 0.0011 0.0045 0.0201 0.0016 0.0034 0.0048 0.0004 0.0039 0.0006 0.0043 0.0018
Vacuole 0.0053 0.0022 0.0034 0.0071 0.0121 0.002 0.0132 0.002 0.0018 0.0033 0.0016 0.002 0.0014 0.0017 0.0019 0.0015 0.0019 0.0008
Vacuole Periphery 0.0029 0.0004 0.0005 0.0013 0.0084 0.0007 0.003 0.0003 0.0005 0.0014 0.0007 0.0004 0.0002 0.0002 0.0002 0.0003 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.5135 59.2672 58.6385 50.9129 66.5163 52.0632 53.8612 51.5197 59.3151 50.3541
Translational Efficiency 2.2373 1.7445 1.8561 2.1747 1.5563 1.8606 1.6557 2.0979 1.6704 1.759

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Tae1

Tae1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tae1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available