Standard name | |
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Human Ortholog | |
Description | AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
Bud | 0 | 1 | – | 1 | 0 | 2 | 1 | – | 3 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 8 |
Bud Neck | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 0 | – | 0 | 0 | 0 | 1 | – | 1 | 4 | 1 | 1 | 2 | 1 | 2 | 7 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 283 | 314 | – | 32 | 65 | 78 | 188 | – | 123 | 124 | 105 | 137 | 341 | 495 | 489 | 190 | 122 | 152 | 276 | 301 | 58 | 191 | 495 | 385 |
Endoplasmic Reticulum | 2 | 2 | – | 0 | 1 | 0 | 0 | – | 2 | 2 | 0 | 4 | 1 | 2 | 1 | 9 | 6 | 5 | 3 | 2 | 0 | 1 | 10 | 4 |
Endosome | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 3 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 8 | 2 | 0 | 1 | 7 | 4 |
Golgi | 2 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 2 | 0 |
Mitochondria | 0 | 1 | – | 0 | 0 | 10 | 24 | – | 59 | 34 | 42 | 49 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 3 | 0 | 0 | 5 | 2 |
Nucleus | 1 | 0 | – | 0 | 1 | 2 | 2 | – | 2 | 9 | 12 | 15 | 5 | 6 | 2 | 8 | 4 | 2 | 0 | 2 | 1 | 0 | 2 | 2 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 2 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 4 | 4 |
Vac/Vac Membrane | 16 | 2 | – | 4 | 1 | 3 | 61 | – | 24 | 17 | 7 | 12 | 9 | 9 | 44 | 8 | 4 | 2 | 1 | 3 | 1 | 8 | 12 | 7 |
Unique Cell Count | 296 | 319 | 36 | 68 | 90 | 245 | 183 | 169 | 149 | 194 | 354 | 505 | 506 | 199 | 127 | 155 | 305 | 324 | 64 | 213 | 563 | 427 | ||
Labelled Cell Count | 312 | 320 | 37 | 68 | 97 | 279 | 216 | 199 | 171 | 219 | 362 | 516 | 538 | 223 | 140 | 166 | 305 | 324 | 64 | 213 | 563 | 427 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.6 | 8.5 | – | 7.3 | 7.9 | 4.7 | 6.2 | – | 5.3 | 5.4 | 5.3 | 5.2 | 9.3 | 8.3 | 8.9 | 11.2 | 11.2 | 11.9 | 8.4 | 8.4 | 8.9 |
Std Deviation (1e-4) | 2.0 | 1.5 | – | 0.9 | 1.2 | 1.1 | 1.4 | – | 1.4 | 1.2 | 1.0 | 0.9 | 1.8 | 1.7 | 1.9 | 2.1 | 2.2 | 2.5 | 2.0 | 1.9 | 2.1 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5037 | 1.0116 | 0.6162 | 0.2101 | 0.2172 | 1.0602 | 2.6129 | 3.0595 | 2.8758 | 2.4186 | 2.2656 | 2.8658 | 4.2347 | 5.6886 | 4.9617 | 4.5651 | 4.9213 | 4.9309 |
Actin | 0.0712 | 0.0005 | 0.0025 | 0.0532 | 0.0697 | 0.0135 | 0.0144 | 0.0016 | 0.0091 | 0.0074 | 0.0217 | 0.0014 | 0.0091 | 0.0002 | 0.0122 | 0.0011 | 0.0009 | 0.0031 |
Bud | 0.0016 | 0.0004 | 0.0002 | 0.0003 | 0.0033 | 0.0005 | 0.0022 | 0.0193 | 0.012 | 0.0243 | 0.002 | 0.0029 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0004 |
Bud Neck | 0.0019 | 0.0001 | 0.002 | 0.0006 | 0.001 | 0.0006 | 0.0034 | 0.0003 | 0.0017 | 0.0003 | 0.0003 | 0.002 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0009 |
Bud Periphery | 0.002 | 0.0002 | 0.0001 | 0.0002 | 0.0042 | 0.0003 | 0.0021 | 0.0078 | 0.0041 | 0.0094 | 0.0025 | 0.001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0005 |
Bud Site | 0.0094 | 0.0008 | 0.0047 | 0.002 | 0.0069 | 0.0002 | 0.0148 | 0.0206 | 0.0183 | 0.0018 | 0.0034 | 0.0011 | 0.0014 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0005 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.5803 | 0.9141 | 0.8342 | 0.7559 | 0.4951 | 0.8941 | 0.5884 | 0.7796 | 0.7237 | 0.7091 | 0.6513 | 0.8803 | 0.6635 | 0.7787 | 0.7157 | 0.8686 | 0.7554 | 0.8149 |
Cytoplasmic Foci | 0.0379 | 0.0071 | 0.018 | 0.0283 | 0.0254 | 0.0112 | 0.0403 | 0.0117 | 0.028 | 0.0457 | 0.0185 | 0.0164 | 0.0109 | 0.0056 | 0.0182 | 0.0114 | 0.0077 | 0.007 |
Eisosomes | 0.0003 | 0 | 0 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0198 | 0.0053 | 0.0143 | 0.008 | 0.017 | 0.0056 | 0.0062 | 0.0032 | 0.0041 | 0.0072 | 0.0026 | 0.0032 | 0.0081 | 0.0033 | 0.0045 | 0.0071 | 0.004 | 0.0021 |
Endosome | 0.0473 | 0.0074 | 0.0114 | 0.0573 | 0.1092 | 0.0136 | 0.0453 | 0.0104 | 0.0178 | 0.016 | 0.0093 | 0.0082 | 0.0082 | 0.0071 | 0.0093 | 0.008 | 0.0154 | 0.0036 |
Golgi | 0.0255 | 0.0005 | 0.0026 | 0.0084 | 0.0435 | 0.0031 | 0.0101 | 0.0034 | 0.0084 | 0.0028 | 0.0072 | 0.0008 | 0.0008 | 0.0002 | 0.0009 | 0.0004 | 0.0008 | 0.0003 |
Lipid Particles | 0.011 | 0.0002 | 0.0009 | 0.0112 | 0.0144 | 0.0019 | 0.0172 | 0.0003 | 0.0026 | 0.0033 | 0.0294 | 0.0001 | 0.0011 | 0.0003 | 0.0009 | 0.0001 | 0.001 | 0.0004 |
Mitochondria | 0.0102 | 0.0002 | 0.0003 | 0.0018 | 0.0392 | 0.0019 | 0.0199 | 0.0018 | 0.0156 | 0.0084 | 0.0468 | 0.0013 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0005 | 0.0002 |
None | 0.1202 | 0.0566 | 0.0937 | 0.0502 | 0.1059 | 0.039 | 0.146 | 0.1324 | 0.1238 | 0.1206 | 0.1659 | 0.0636 | 0.2838 | 0.1987 | 0.226 | 0.0948 | 0.1935 | 0.1601 |
Nuclear Periphery | 0.014 | 0.0017 | 0.0036 | 0.0039 | 0.0288 | 0.0043 | 0.0123 | 0.0009 | 0.0029 | 0.0048 | 0.001 | 0.0016 | 0.0029 | 0.0009 | 0.0015 | 0.0017 | 0.0079 | 0.0006 |
Nucleolus | 0.0005 | 0 | 0.0001 | 0.001 | 0.0009 | 0.0001 | 0.0044 | 0.0003 | 0.001 | 0.0008 | 0.0011 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 |
Nucleus | 0.0041 | 0.0017 | 0.0021 | 0.0033 | 0.0066 | 0.0051 | 0.0278 | 0.0023 | 0.0144 | 0.0054 | 0.0012 | 0.0077 | 0.0019 | 0.0019 | 0.002 | 0.0029 | 0.0048 | 0.0018 |
Peroxisomes | 0.0157 | 0.0001 | 0.0044 | 0.0033 | 0.0042 | 0.0014 | 0.0107 | 0.0002 | 0.0053 | 0.0078 | 0.0315 | 0.0024 | 0.0002 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0007 |
Punctate Nuclear | 0.0186 | 0.0004 | 0.0008 | 0.0024 | 0.0032 | 0.0012 | 0.0178 | 0.0011 | 0.0045 | 0.0201 | 0.0016 | 0.0034 | 0.0048 | 0.0004 | 0.0039 | 0.0006 | 0.0043 | 0.0018 |
Vacuole | 0.0053 | 0.0022 | 0.0034 | 0.0071 | 0.0121 | 0.002 | 0.0132 | 0.002 | 0.0018 | 0.0033 | 0.0016 | 0.002 | 0.0014 | 0.0017 | 0.0019 | 0.0015 | 0.0019 | 0.0008 |
Vacuole Periphery | 0.0029 | 0.0004 | 0.0005 | 0.0013 | 0.0084 | 0.0007 | 0.003 | 0.0003 | 0.0005 | 0.0014 | 0.0007 | 0.0004 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.5135 | 59.2672 | 58.6385 | 50.9129 | 66.5163 | 52.0632 | 53.8612 | 51.5197 | 59.3151 | 50.3541 |
Translational Efficiency | 2.2373 | 1.7445 | 1.8561 | 2.1747 | 1.5563 | 1.8606 | 1.6557 | 2.0979 | 1.6704 | 1.759 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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