Standard name | |
---|---|
Human Ortholog | |
Description | Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 0 | 0 | 2 |
Bud | 1 | 0 | 0 | 0 | 1 | 5 | 8 | 12 | – | 7 | 10 | 6 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 4 | 3 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | – | 0 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 3 | 3 | 2 | 2 | 4 | 3 | 3 | – | 2 | 2 | 4 | 0 | 0 | 0 | 10 | 7 | 17 | 0 | 2 | 2 | 0 | 0 | 0 |
Cytoplasm | 434 | 417 | 219 | 229 | 313 | 343 | 392 | 387 | – | 257 | 183 | 239 | 157 | 213 | 231 | 318 | 262 | 232 | 418 | 382 | 195 | 100 | 165 | 191 |
Endoplasmic Reticulum | 2 | 4 | 0 | 0 | 1 | 1 | 2 | 2 | – | 1 | 0 | 1 | 3 | 1 | 2 | 11 | 9 | 8 | 1 | 1 | 1 | 2 | 2 | 1 |
Endosome | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 7 | – | 1 | 1 | 2 | 1 | 1 | 2 | 0 | 1 | 1 | 5 | 2 | 4 | 1 | 1 | 5 |
Golgi | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 7 | 0 | 0 | 4 | 1 | 0 |
Mitochondria | 4 | 3 | 13 | 8 | 34 | 138 | 225 | 246 | – | 178 | 169 | 235 | 0 | 1 | 0 | 6 | 2 | 2 | 10 | 11 | 22 | 5 | 7 | 7 |
Nucleus | 1 | 1 | 0 | 0 | 1 | 2 | 3 | 5 | – | 5 | 0 | 3 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 0 | 2 | 3 | 4 | – | 5 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 1 | 3 |
Vac/Vac Membrane | 1 | 3 | 0 | 1 | 6 | 1 | 6 | 10 | – | 16 | 2 | 1 | 0 | 1 | 0 | 3 | 4 | 5 | 7 | 5 | 2 | 3 | 2 | 4 |
Unique Cell Count | 442 | 424 | 232 | 232 | 329 | 403 | 518 | 534 | 388 | 282 | 379 | 162 | 219 | 236 | 325 | 268 | 237 | 466 | 424 | 242 | 126 | 194 | 229 | |
Labelled Cell Count | 447 | 433 | 241 | 241 | 359 | 500 | 646 | 680 | 475 | 375 | 495 | 163 | 221 | 238 | 350 | 287 | 267 | 466 | 424 | 242 | 126 | 194 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 4.6 | 3.9 | 4.1 | 3.9 | 3.6 | 3.3 | 3.4 | – | 3.2 | 3.4 | 3.1 | 5.1 | 5.1 | 5.1 | 6.5 | 6.4 | 6.6 | 6.2 | 5.9 | 6.3 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.3 | 1.9 | 1.6 | 1.5 | 1.4 | 1.3 | – | 1.1 | 1.6 | 1.2 | 0.9 | 1.1 | 1.3 | 1.1 | 1.4 | 1.5 | 2.5 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.04 | -0.01 | -0.13 | -0.25 | -0.23 | – | -0.32 | -0.22 | -0.34 | 0.36 | 0.36 | 0.37 | 0.73 | 0.7 | 0.75 | 0.65 | 0.57 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.4795 | 0.9176 | 0.7209 | 0.5525 | 0.8694 | 0.8283 | 2.5239 | 2.6464 | 2.5102 | 1.9999 | 2.0036 | 2.7498 | -0.4988 | 0.1243 | -0.1009 | -0.0042 | -0.0169 | -0.0928 |
Actin | 0.0013 | 0.0026 | 0.0011 | 0.0027 | 0.0032 | 0.0024 | 0.0474 | 0.0001 | 0.0121 | 0.0004 | 0.0041 | 0.0064 | 0.0008 | 0.0024 | 0.0004 | 0.0001 | 0.0001 | 0.0006 |
Bud | 0.0001 | 0.0017 | 0.0003 | 0.0001 | 0.0034 | 0.0002 | 0.0007 | 0.0001 | 0.0014 | 0.005 | 0.0048 | 0.0012 | 0.0001 | 0.0012 | 0.0004 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0016 | 0.0009 | 0.0041 | 0.0002 | 0.0027 | 0.0001 | 0.0007 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Bud Periphery | 0.0001 | 0.001 | 0.0004 | 0.0002 | 0.0051 | 0.0005 | 0.0014 | 0.0001 | 0.0021 | 0.0154 | 0.0252 | 0.0031 | 0.0001 | 0.0019 | 0.0003 | 0.0001 | 0 | 0.0003 |
Bud Site | 0.0009 | 0.0129 | 0.0008 | 0.0001 | 0.0052 | 0.0001 | 0.0049 | 0.0023 | 0.003 | 0.0001 | 0.0034 | 0.0002 | 0.0006 | 0.0107 | 0.0003 | 0.0001 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.1766 | 0.2957 | 0.2283 | 0.2424 | 0.2951 | 0.2731 | 0.1455 | 0.3226 | 0.1946 | 0.1659 | 0.3332 | 0.2393 | 0.1977 | 0.312 | 0.2529 | 0.4412 | 0.2489 | 0.3727 |
Cytoplasmic Foci | 0.006 | 0.0075 | 0.0056 | 0.0054 | 0.0355 | 0.0064 | 0.0063 | 0.0072 | 0.0158 | 0.0014 | 0.0157 | 0.0094 | 0.0106 | 0.0049 | 0.0063 | 0.009 | 0.003 | 0.0057 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0021 | 0.0021 | 0.002 | 0.0011 | 0.0025 | 0.001 | 0.0017 | 0.0011 | 0.0009 | 0.0007 | 0.0012 | 0.0014 | 0.0011 | 0.0017 | 0.0015 | 0.001 | 0.0003 | 0.0005 |
Endosome | 0.002 | 0.004 | 0.0018 | 0.0022 | 0.0119 | 0.002 | 0.0079 | 0.0021 | 0.0029 | 0.0004 | 0.0052 | 0.0013 | 0.0038 | 0.0013 | 0.0032 | 0.0025 | 0.0004 | 0.001 |
Golgi | 0.0005 | 0.0004 | 0.0002 | 0.0002 | 0.0084 | 0.0003 | 0.0022 | 0.0002 | 0.0016 | 0 | 0.0037 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0 | 0 |
Lipid Particles | 0.0011 | 0.0003 | 0.0002 | 0.0001 | 0.0028 | 0.0004 | 0.0021 | 0.0001 | 0.0015 | 0 | 0.0015 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Mitochondria | 0.0005 | 0.0003 | 0.0003 | 0.0004 | 0.0103 | 0.0003 | 0.0074 | 0.0003 | 0.0007 | 0.0011 | 0.0033 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0001 |
None | 0.7951 | 0.6649 | 0.7539 | 0.7404 | 0.6012 | 0.7088 | 0.7451 | 0.6593 | 0.7494 | 0.7988 | 0.5785 | 0.732 | 0.7761 | 0.6567 | 0.7257 | 0.5419 | 0.7449 | 0.6162 |
Nuclear Periphery | 0.0048 | 0.0008 | 0.0008 | 0.0008 | 0.001 | 0.0005 | 0.002 | 0.0008 | 0.0005 | 0.0021 | 0.0009 | 0.0008 | 0.0012 | 0.0016 | 0.0007 | 0.0004 | 0.0002 | 0.0002 |
Nucleolus | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0012 | 0.0001 | 0.0003 | 0.0005 | 0.0008 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0 | 0 |
Nucleus | 0.0036 | 0.0022 | 0.0014 | 0.0015 | 0.0018 | 0.0011 | 0.0078 | 0.0012 | 0.0008 | 0.0046 | 0.0015 | 0.0009 | 0.0013 | 0.0018 | 0.003 | 0.0015 | 0.0008 | 0.0008 |
Peroxisomes | 0.0029 | 0.0005 | 0.0002 | 0.0004 | 0.0054 | 0.0002 | 0.0046 | 0.0001 | 0.006 | 0.0001 | 0.0106 | 0.0017 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0006 | 0.0011 | 0.0016 | 0.0008 | 0.0029 | 0.0011 | 0.002 | 0.0008 | 0.0027 | 0.0004 | 0.0018 | 0.0005 | 0.0034 | 0.0011 | 0.0032 | 0.0006 | 0.0006 | 0.0008 |
Vacuole | 0.0009 | 0.0008 | 0.0005 | 0.0007 | 0.0019 | 0.0004 | 0.0046 | 0.001 | 0.0006 | 0.0016 | 0.0025 | 0.0003 | 0.0005 | 0.0007 | 0.0007 | 0.0004 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0008 | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.3096 | 16.4396 | 9.7591 | 20.4871 | 20.267 | 18.3799 | 23.6864 | 26.3641 | 22.2532 | 22.3895 |
Translational Efficiency | 1.6025 | 1.7649 | 2.2724 | 1.15 | 1.6591 | 1.4813 | 1.4136 | 0.9729 | 1.0567 | 1.3433 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|