Standard name | |
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Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 2 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | – | – | 0 | 4 | 3 | 0 | 0 | 3 | 5 |
Bud | 4 | 2 | 6 | 15 | 7 | 2 | – | 6 | 11 | 13 | 4 | 16 | 2 | 1 | 1 | – | – | 1 | 2 | 0 | 0 | 0 | 0 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 2 | 1 | 1 | 4 | – | 5 | 2 | 7 | 3 | 5 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 10 | 6 | 3 | 4 | 2 | – | 1 | 0 | 2 | 1 | 2 | 1 | 5 | 1 | – | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 4 | 4 | 11 | 5 | – | 7 | 8 | 2 | 7 | 4 | 0 | 3 | 3 | – | – | 3 | 0 | 1 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 3 | 2 | 3 | 2 | 0 | 3 | – | 1 | 2 | 1 | 2 | 0 | 1 | 1 | 4 | – | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 5 | 0 | 0 | 1 | 22 | 4 | – | 5 | 1 | 3 | 1 | 0 | 3 | 7 | 13 | – | – | 0 | 10 | 6 | 11 | 2 | 8 | 7 |
Golgi | 6 | 20 | 2 | 0 | 2 | 1 | – | 0 | 0 | 0 | 0 | 0 | 5 | 12 | 16 | – | – | 3 | 15 | 8 | 5 | 3 | 17 | 15 |
Mitochondria | 263 | 195 | 193 | 328 | 335 | 257 | – | 302 | 314 | 319 | 248 | 463 | 119 | 232 | 204 | – | – | 3 | 226 | 196 | 159 | 45 | 114 | 115 |
Nucleus | 1 | 0 | 0 | 0 | 2 | 1 | – | 7 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 13 | 9 | 11 | 10 | 19 | 27 | – | 28 | 42 | 33 | 35 | 42 | 11 | 21 | 17 | – | – | 1 | 3 | 1 | 3 | 0 | 4 | 2 |
Nucleolus | 10 | 9 | 5 | 16 | 21 | 23 | – | 40 | 31 | 24 | 16 | 38 | 13 | 12 | 13 | – | – | 0 | 2 | 0 | 0 | 0 | 0 | 3 |
Peroxisomes | 5 | 1 | 0 | 9 | 20 | 2 | – | 3 | 9 | 1 | 5 | 3 | 4 | 6 | 6 | – | – | 2 | 4 | 4 | 9 | 4 | 11 | 11 |
SpindlePole | 0 | 1 | 1 | 1 | 3 | 2 | – | 9 | 10 | 2 | 4 | 12 | 3 | 1 | 6 | – | – | 1 | 0 | 0 | 2 | 0 | 3 | 1 |
Vac/Vac Membrane | 60 | 43 | 14 | 14 | 32 | 17 | – | 10 | 15 | 10 | 13 | 7 | 22 | 33 | 29 | – | – | 3 | 20 | 11 | 6 | 2 | 9 | 13 |
Unique Cell Count | 289 | 228 | 202 | 348 | 380 | 278 | 339 | 345 | 334 | 266 | 479 | 137 | 260 | 244 | 18 | 298 | 238 | 203 | 62 | 177 | 186 | |||
Labelled Cell Count | 376 | 297 | 249 | 404 | 479 | 350 | 424 | 446 | 418 | 341 | 593 | 184 | 337 | 313 | 19 | 298 | 238 | 203 | 62 | 177 | 186 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.8 | 9.8 | 5.2 | 6.2 | 6.8 | 5.8 | – | 5.4 | 5.3 | 5.2 | 5.5 | 5.0 | 7.5 | 8.0 | 7.8 | – | – | 10.3 | 7.6 | 8.6 | 8.7 |
Std Deviation (1e-4) | 1.7 | 1.8 | 1.2 | 2.3 | 1.6 | 2.4 | – | 1.4 | 2.2 | 1.3 | 2.4 | 1.2 | 1.3 | 1.4 | 1.4 | – | – | 3.2 | 1.9 | 1.9 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.27 | 0.39 | 0.17 | – | 0.07 | 0.05 | 0.0 | 0.1 | -0.05 | 0.53 | 0.64 | 0.59 | – | – | 1.0 | 0.56 | 0.73 | 0.75 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud | 0.8 | 0 | 0 | – | 0 | 0.1 | 0.6 | 0 | 0.2 | 0 | 0 | 0 | – | – | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cytoplasm | 0 | 0.7 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Endosome | 0 | 3.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | – | – | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 3.0 | – | – | 0 |
Mitochondria | -0.7 | -2.9 | -1.4 | – | -2.6 | -2.0 | 0 | -1.1 | 0.7 | -2.9 | -2.5 | -4.0 | – | – | -10.3 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nuclear Periphery | -1.5 | -0.2 | 1.7 | – | 1.2 | 2.6 | 1.8 | 2.8 | 1.5 | 0.9 | 1.1 | 0.7 | – | – | 0 |
Nucleolus | 1.3 | 1.7 | 2.7 | – | 3.8 | 3.0 | 2.3 | 1.8 | 2.7 | 2.8 | 1.2 | 1.5 | – | – | 0 |
Peroxisomes | 0 | 3.3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Vacuole | -1.5 | 0.6 | -0.4 | – | -2.2 | -1.3 | -2.1 | -0.9 | -3.8 | 2.7 | 2.0 | 1.8 | – | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.4709 | 4.4518 | 5.0866 | 4.1449 | 4.6374 | 5.141 | 3.9173 | 4.1852 | 3.8573 | 3.286 | 4.2222 | 4.3423 | 5.9823 | 6.4484 | 5.6969 | 6.1619 | 6.914 | 5.4527 |
Actin | 0.0073 | 0.013 | 0.0009 | 0.003 | 0.0123 | 0.0017 | 0.0119 | 0.0018 | 0.0089 | 0.0327 | 0.0063 | 0.0054 | 0.0488 | 0.0052 | 0.0153 | 0.0177 | 0.0322 | 0.0011 |
Bud | 0.0001 | 0.001 | 0.0001 | 0.0015 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0013 | 0.0003 | 0.0001 | 0.0005 | 0.0004 | 0.0007 | 0.0006 | 0.0003 | 0.0001 |
Bud Neck | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0003 | 0.0014 | 0.002 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0001 |
Bud Periphery | 0.0002 | 0.0019 | 0.0002 | 0.0042 | 0.0004 | 0.0002 | 0.0006 | 0.0003 | 0.0006 | 0.0014 | 0.0005 | 0.0002 | 0.001 | 0.001 | 0.001 | 0.0015 | 0.0006 | 0.0002 |
Bud Site | 0.0002 | 0.0022 | 0.0001 | 0.0053 | 0.0001 | 0.0001 | 0.0011 | 0.0005 | 0.0008 | 0.0047 | 0.0009 | 0.0001 | 0.0055 | 0.0013 | 0.0017 | 0.0009 | 0.0006 | 0.0001 |
Cell Periphery | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0014 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0005 | 0.0003 | 0.0005 | 0.0001 |
Cytoplasm | 0 | 0.0001 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0069 | 0.0002 | 0.0003 | 0.0015 | 0.001 | 0.0002 | 0.0008 | 0.0004 | 0.0004 | 0.0001 | 0.0008 | 0.0038 |
Cytoplasmic Foci | 0.004 | 0.004 | 0.002 | 0.0101 | 0.0055 | 0.0024 | 0.0094 | 0.0045 | 0.0079 | 0.0221 | 0.016 | 0.0037 | 0.0086 | 0.0056 | 0.0044 | 0.0023 | 0.0075 | 0.0033 |
Eisosomes | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0012 | 0.0033 | 0.0001 |
Endoplasmic Reticulum | 0.0001 | 0.0001 | 0.0009 | 0.0002 | 0.0003 | 0.0003 | 0.0024 | 0.0001 | 0.0004 | 0.0005 | 0.0019 | 0.0003 | 0.001 | 0.0002 | 0.0009 | 0.0002 | 0.0028 | 0.0011 |
Endosome | 0.0145 | 0.0286 | 0.0079 | 0.0101 | 0.0147 | 0.0141 | 0.0303 | 0.0149 | 0.0254 | 0.0263 | 0.0447 | 0.0144 | 0.013 | 0.0217 | 0.0142 | 0.0056 | 0.0241 | 0.0091 |
Golgi | 0.0251 | 0.0249 | 0.0235 | 0.0184 | 0.0121 | 0.0201 | 0.0371 | 0.0341 | 0.0241 | 0.0238 | 0.0406 | 0.024 | 0.0202 | 0.0769 | 0.0179 | 0.0046 | 0.0209 | 0.0067 |
Lipid Particles | 0.0281 | 0.0302 | 0.0079 | 0.0079 | 0.0143 | 0.013 | 0.0274 | 0.0024 | 0.0155 | 0.0311 | 0.0187 | 0.0097 | 0.0434 | 0.0134 | 0.0212 | 0.0088 | 0.0181 | 0.0107 |
Mitochondria | 0.8942 | 0.8484 | 0.9435 | 0.9176 | 0.9146 | 0.9315 | 0.828 | 0.9283 | 0.891 | 0.8324 | 0.8394 | 0.9199 | 0.8258 | 0.8413 | 0.8812 | 0.9171 | 0.8534 | 0.9432 |
None | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0005 | 0.0001 | 0.0011 | 0 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0012 | 0.0001 | 0.0007 | 0.0005 | 0.0025 | 0.0003 |
Nuclear Periphery | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0129 | 0 | 0.0004 | 0.0003 | 0.0036 | 0.0013 | 0.0011 | 0.0006 | 0.0005 | 0.0002 | 0.0081 | 0.0015 |
Nucleolus | 0.0001 | 0.0002 | 0 | 0.0025 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0025 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0013 | 0.0006 | 0.0004 | 0.0002 |
Nucleus | 0.0001 | 0.0002 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0019 | 0 | 0.0001 | 0.0005 | 0.0011 | 0.0001 | 0.0003 | 0.0002 | 0.0007 | 0.0003 | 0.0009 | 0.0008 |
Peroxisomes | 0.018 | 0.0159 | 0.007 | 0.0051 | 0.0058 | 0.0052 | 0.0147 | 0.0099 | 0.0182 | 0.0103 | 0.0186 | 0.0128 | 0.0186 | 0.0142 | 0.019 | 0.0261 | 0.0133 | 0.0126 |
Punctate Nuclear | 0 | 0 | 0 | 0.004 | 0.0019 | 0 | 0.0009 | 0 | 0.0005 | 0.0006 | 0.0018 | 0.0003 | 0.0018 | 0 | 0.0003 | 0.0001 | 0.0037 | 0.0005 |
Vacuole | 0.0021 | 0.0089 | 0.0013 | 0.0036 | 0.0034 | 0.0016 | 0.003 | 0.0007 | 0.0016 | 0.004 | 0.0013 | 0.0012 | 0.0018 | 0.0041 | 0.0075 | 0.0068 | 0.0015 | 0.001 |
Vacuole Periphery | 0.0054 | 0.0195 | 0.0037 | 0.0029 | 0.0123 | 0.0089 | 0.0091 | 0.0016 | 0.002 | 0.0018 | 0.0021 | 0.0041 | 0.0035 | 0.0125 | 0.0099 | 0.0043 | 0.0041 | 0.0034 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.3728 | 9.3054 | 32.2235 | 35.5376 | 49.4866 | 20.8074 | 36.0667 | 48.6852 | 60.7709 | 50.1767 |
Translational Efficiency | 1.7897 | 7.9389 | 1.8213 | 2.0802 | 1.594 | 5.6974 | 2.6042 | 2.6353 | 1.9303 | 2.2532 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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